This represents a major investment at the University of Liverpool for research and training in X-ray structural biology and molecular biophysics. We provide postgraduates and postdoctoral staff with opportunities for cross-disciplinary research, enhancing their prospects for work in industries applying X-ray techniques.
The facility houses the following instruments:
Rigaku FR-E+ Superbright microfocus rotating anode generator
the brightest available desk laboratory X-ray source, comparable to 2nd generation synchrotron bending magnet beamlines. It has the following specifications:
- Operates in a distinctive dual port mode.
- One X-ray port is a state-of-the-art set up for laboratory-based protein crystallography using a MAR desktop beamline equipped with a cryogenic robotic sample changer for automated data collection and crystal screening functions. A Rayonix MX-225 3 x 3 CCD detector is used for collecting diffraction data.
- The second X-ray port is for small angle X-ray scattering (SAXS) experiments, where Size Exclusion Chromatography (SEC) and Light Scattering are combined to provide a SEC-SAXS facility. It has a maximum camera length of 3 metres, corresponding to a d-spacing of ~1900 Å, and is equipped with a MAR300 image plate detector.
Single crystal microspectrophotometer
The single crystal microspectrophotometer is made to measure UV/visible spectra of protein crystals at 100 K.
The laboratory is named in honour of Charles Glover Barkla. A student and lecturer at the university, Barkla received the 1917 Nobel Prize in Physics for his key contributions to defining the nature of X-rays, which led to the first X-ray diffraction experiments, and for studying interactions of X-rays with matter, which led to the identification of discrete electron energy states (K, L, M...) in atoms.