Spatial Profiling, also known as ‘spatially resolved transcriptomics’, was voted Nature’s method of the year in 2020 due to the versatility of the technology to address many research questions across a wide variety of areas – Oncology, Neuroscience, Infectious Disease, Inflammation and Immunity.

Spatial profiling uses a combination of immunofluorescence microscopy and next-generation sequencing to quantify gene and/or protein expression in distinct areas of your tissue.

What you'll receive from our facility

Within the facility we currently use Nanostring’s GeoMx Digital Spatial Profiler.  This instrument enables users to:

  • Work with FFPE, Fresh Frozen, Tissue Microarrays or slide mounted cell-culture and organoid models; Use a standard IHC workflow to prepare slides for Spatial Profiling; Profile multiple, user defined Regions of Interest (ROIs) across their tissue sections; Use immunofluorescence to drive spatial profiling from distinct tissue regions (Tumour vs TME or white vs grey matter) or specific cell populations (B-cells, T-cells, macrophages, microglia etc); Understand Differential Gene Expression across cell types, tissue areas and across different disease states.

The equipment we offer includes:

  • Nanostring’s GeoMx Digital Spatial Profiler
  • Nanostring’s nCounter Pro Analysis System
  • Leica Bond RXm
  • HybEZ II Hybridisation System.

Who can use our facilities

  • University of Liverpool academic staff
  • Researchers from other universities/ research institutions
  • Industrial research partners.

What Spatial Profiling can be used for

  • Project consultation on spatial proteogenomic projects
  • Optimisation of sample preparation and fluorescent morphology marker staining for Region of interest (ROI selection)
  • Library preparation for nanostring GeoMx assays 
  • Sample preparation and training for nanostring nCounter Pro System.


Back to: Research

Our experts

Dr Frances Greaney Davies

Spatial Profiling Lab Manager and Liverpool University Biobank Scientific Lead

Find us

Liverpool University Biobank
3rd Floor William Henry Duncan Building
6 West Derby Street
L7 8TX