The University of Liverpool's Centre for Cell Imaging (CCI) is a world-class resource for imaging projects, a member of the UK Node of Euro-BioImaging and is listed in the Royal Microscopy Society national database.
The Centre provides researchers with a state of the art suite of custom-designed temperature-controlled rooms for optimal live cell imaging.
The facility’s next-generation equipment and resources are supported by experienced and knowledgeable technical specialists and renowned academics to help you make the most of your data.
All microscopes have temperature and gas control to maintain cells or organisms alive in their physiological environment
Our facility staff are available to share their expertise in imaging non-biological samples such as hydrogels, cage nanocrystals and antibacterial surfaces
We are also able to offer support in: Planning and study design; grant applications (preliminary data analysis, figure preparation, method development, data management planning and costings); analysis including method development, data acquisition, associated software training and support and dissemination including outreach and stakeholder support, manuscript preparation and data storage and curation
Training is available including 1-to-1 hands-on at our facility, online training, group training or face to face workshops and pre-recorded training.
The equipment we offer includes:
Several confocal microscopes
Two spinning disk confocal microscopes
Lightsheet microscope for 3D/4D imaging
Several super-resolution techniques
Atomic force microscopy combined with fluorescence (bioAFM)
Total internal reflection fluorescence (TIRF)
Specialist “F-techniques”, e.g. Fluorescence lifetime imaging microscopy (FLIM) Medium and high throughput imaging systems.
We specialise in a range of microscopy techniques including confocal, epifluorescent, lightsheet, high-content, atomic force and super-resolution light microscopy, as well as having expertise in post-acquisition analysis.
All of our systems can be set to maintain sample CO2, temperature and humidity with some of them capable of maintaining low O2 for hypoxic studies.
The whole of the facility is classed as a containment level 2 (CL2) laboratory space and can cater for biological samples up to hazard group 2 (HG2), pending receipt of a health and safety office approved risk assessment.
Our capabilities include:
High quality imaging of fixed samples in 2D/3D
Automated, long-term live cell imaging with environmental control
Super resolution imaging including Lattice SIM2, PALM/STORM, SRRF, SOFI
Deep tissue imaging and second harmonic generation (multiphoton)
Imaging across scales from single molecule to cells and model organisms
Phase contrast, dark field, oblique and phase gradient imaging of transmitted light
Image analysis and support with state-of-the-art software packages and an on-site image analyst
CCI Biosciences Hub
Biosciences Building
Crown Street
Liverpool L69 7BE
CCI Nuffield Hub
Nuffield Building
Crown Street
University of Liverpool
Liverpool L69 3BX