Protein Crystallisation Facility

The Protein Crystallisation Facility at the Institute of Integrative Biology provides state of the art capability and expertise to help researchers study the three-dimensional structures of biomolecules.

X-ray crystallography provides highly accurate, three-dimensional images of biological macromolecules and their complexes at atomic resolution. This information is essential to help support research into the design of new drugs targeted at human diseases, bio-catalysis, synthetic biology, drug toxicology, and other multidisciplinary research areas.

The facility

  • Starlet Hamilton liquid-dispenser for the preparation of reservoir solutions to complement a collection of commercial screens
  • Innovadyne Screenmaker 96+8 crystallization robot operating at nano-volumes (working range 50nl-1µl; 100 nl typical operational mode)
  • Large capacity Minstrel hotel for remote automated visualization of protein drops with 5M pixel uv/vis resolution, archiving of crystal growth results, and ability to distinguish between false salt and biological crystals
  • Vibration free incubators available for storage of plates at 4°C.

Our history

To facilitate the interdisciplinary applications of X-ray crystallography, the University of Liverpool opened the Protein Crystallisation Facility in September 2012 to complement the Barkla X-ray Laboratory of Biophysics opened in July 2011.

Collaborations

The crystallisation facility is open to all researchers working on crystallogenesis trials of their proteins. We are also open for collaborative projects including access to Barkla X-ray Laboratory of Biophysics and Beam Allocation Groups at SOLEIL and DIAMOND.

We are happy to discuss projects at an early stage where assistance with the production of protein samples in conditions compatible with the X-ray crystallography technique are needed.

Contact

For further information or an informal discussion on strategies tailored to individual projects, please contact Dr Svetlana Antonyuk and/or Mrs Carol Beesley.