Final program

Day 1, Tuesday 30 June

17:00 - 18:30

Breakout area

REGISTRATION, WELCOME RECEPTION AND POSTER SET-UP

Enjoy a drink and canapes while connecting with fellow participants

18:30 - 18:45

Lecture Theatre 2

18:45 - 19:45

Lecture Theatre 2

Keynote Lectures

Chair: Judy Coulson

• K-01 Domenico Ribatti, University of Bari, Italy - An historical overview of the use of the CAM assay in the study of tumor angiogenesis
• K-02 Megan Davey, University of Edinburgh, UK - Genetically modified eggs and opportunities in CAM research

Day 2, Wednesday 1 July

 DELEGATE ARRIVAL / POSTER SET-UP

09:25 - 09:30

Lecture Theatre 2

 Welcome, housekeeping, and announcements

09:30 - 10:30

Lecture Theatre 2

Session one: Non-oncological models

Chair: Pedro Gomes, Co-chair: Ayca Uvez

• S1-01 Plenary: Nassim Ghaffari Tabrizi-Wizsy, University of Graz, Austria - From Trophoblast Invasion to Intervillous Perfusion: A Refined Ex Ovo CAM Model of Early Human Placentation
• S1-02 Invited short talk: Pedro Gomes, University of Porto, Portugal - Ex vivo embryonic chick femur culture as a platform to study osteogenesis 
• S1-03 Selected short talk: Zeudi Petrone, University of Naples, Italy - Diabetic Corneal Damage in the Chick Embryo model: A Platform for Ocular Safety and Therapeutic Screening 
• S1-04 Selected short talk: Judith Rees, Deutsches Herzzentrum der Charité, Germany - Using the incubated Chicken Egg as a novel alternative tool in basic cardiovascular electrophysiological research

10:30 - 11:00

Breakout area & Flex2 

11:00 - 12:00

Lecture Theatre 2

Session two: Regenerative medicine and biomaterials

Chair: Veronika Huntošová, Co-chair: Johanna Buschmann

• S2-01 Plenary: Eva Petrovova, University of Veterinary Medicine and Pharmacy in Kosice, Slovakia - Chorioallantoic Membrane – Alternative Model for Biomaterial Testing
• S2-02 Invited short talk: Veronika Huntošová, University in Košice, Slovakia - Multimodal MOF Based Nanoplatform for Localised Photodynamic and Combination Therapy Evaluated in the Quail CAM Model
• S2-03 Selected short talk: Rita Araujo, University of Porto, Portugal - Development of a Controlled Burn Injury Model on the Chicken Chorioallantoic Membrane (CAM)
• S2-04 Selected short talk: Jiao Jin, University College London, UK - Evaluating the Chick Chorioallantoic Membrane (CAM) as a Biomimetic and Multifunctional Platform for the Performance Assessment of Orodispersible Films (ODFs)

12:00 - 12:30

Lecture Theatre 2

Poster flask talks 1

Chair: Niall Kenneth

• F1-01 Mariana Máčajová, Centre of Biosciences SAS, Slovakia -Antimicrobial Sonodynamic Therapy Evaluated using Quail Chorioallantoic Membrane Model 

• F1-02 Şima Mertol, Istanbul University-Cerrahpasa, Türkiye - Promising Therapeutic Potential of p90RSK Inhibitors in Angiogenesis Modulation 

• F1-03 Gabriella Meier Bürgisser, University Hospital Zurich, Switzerland - Synergistic Effects of Insulin-like Growth Factor-1 and Platelet-Derived Growth Factor-BB in Tendon Healing 

• F1-04 Luiz Fernando Pereira, Pontifical Catholic University of Parana, Brazil - Copper–decavanadate complexes promote vascular remodeling and extracellular matrix maturation in the chorioallantoic membrane model 

• F1-05 Nimeet Desai, University of Liverpool, UK - Bioengineering a Mucoadhesive Composite for Ocular Chemotherapy: Preliminary Validation in a Novel CAM Model of Conjunctival Melanoma 

• F1-06 Nattapak Darumas,University of Liverpool, UK - Irritation and angiogenic property evaluation of gallic acid-crosslinked collagen scaffolds using the chick chorioallantoic membrane model 

• F1-07 Anca Maria Cimpean, “Victor Babes” University of Medicine and Pharmacy Timisoara, Romania - Dental biomaterials implanted on chick embryo chorioallantoic membrane: from microscopy to AI based IKOSA and QuPath analysis 

• F1-08 Miguel Angel Merlos Rodrigo, Mendel University in Brno, Czechia - Chorioallantoic Membrane Assay as an Alternative In Vivo Model for Investigating Chemoresistance and Novel Therapeutic Strategies in Triple-Negative Breast Cancer 

• F1-09 Natalia Maciejewska, Gdansk University of Technology, Poland- Anthraquinone - Based Telomerase Inhibition Suppresses Lung Cancer Growth and Invasive Dynamics in 3D and In Vivo Models 

• F1-10 Laura Bowker, University of Liverpool, UK - Interrogating Hippo Pathway Dependencies in Pleural Mesothelioma to Uncover Novel Therapeutic Opportunities: From in vitro to in ovo

12:30 - 13:30

Breakout area & Flex2

LUNCH AND POSTER VIEWING 

13:30 - 14:30

Breakout area & Flex2 

Workshop 1: The 3RS and sustainability in CAM research

Chair: Sarah Barnett

• W1-01 Jenna Lowe, University of Liverpool, UK - LEAF: Laboratory Efficiency Assessment Framework
• TBC
• TBC
• Group discussions

14:20 - 15:00

Lecture Theatre 2

Workshop 2: Enabling uptake of CAM research

Chair: Marta Teixeira Pinto

• W2-01 Charlotte Colle, Capstone DC, UK - New Alternative Methods (NAMs) in Pharma
• Marta Teixeira Pinto, i3S, Porto, Portugal - Evaluating the CAM model as an alternative to murine models in biomedical research: a systematic review of comparative studies
• Group discussions

15:00 - 15:30

Lecture Theatre 2

Poster flask talks 2

Chair: Ann Hopkins

• F2-01 Valeriia Katiuzhynska, University of Liverpool, UK - Developing Personalised Brain Tumour Avatars Using Human Meningioma and Brain Metastases in the Chicken Chorioallantoic Membrane (CAM) Model 

• F2-02 Tobias Faehling, German Cancer Research Center, Germany - Establishing Systemic Doxycycline Delivery in the CAM Model Enables Functional Interrogation of Ewing Sarcoma Fusion Oncogene Dependency 

• F2-03 Niklas Feuchter, University Hospital Erlangen, Germany - Investigating a nanoparticle-based mRNA vaccination platform against multicellular solid tumor spheroids in the chorioallantoic membrane (CAM) model 

• F2-04 Christian Ballmeyer, University Hospital and Medical Faculty of the Heinrich-Heine-University Duesseldorf, Germany - Beyond the Burn: Investigation of Tissue Destruction and CTC Dissemination upon Thermoablation of Pancreatic Cancer on the CAM 

• F2-05 Simon Allison, University of Huddersfield, UK -Development of 14-day, non-sentient in ovo human tumour xenograft models for cancer drug discovery and 3Rs pre-clinical in vivo proof-of-concept 

• F2-06 Lucas Carnell, University Clinic Erlangen, Germany - Magnetic targeting of SPION-functionalized CAR-T cells in vascularized 2D and 3D melanoma models on the chorioallantoic membrane (CAM) 

• F2-07 Mareike Gruber, University Hospital Tübingen, Germany - Hyperspectral imaging of patient-derived endometriotic microtissues integrated in the chorioallantoic membrane model 

• F2-08 Julia Hurnikova, University of Veterinary Medicine and Pharmacy in Kosice, Slovakia - Evaluation of rAAV Vector Visualization and Delivery Strategies Using the Chick Chorioallantoic Membrane (CAM) Model 

• F2-09 Qin Pu, University Hospital Bonn, Germany - Validated AI-Based Quantification of Superficial Chorioallantoic Membrane Vasculature 

• F2-10 Marta Pomraenke, Jena University Hospital, Germany - The ostrich chorioallantoic model for in vivo imaging of cancer xenografts using a human PET/CT scanner 

15:30 - 17:00

Flex2

Poster Mixer 

POSTER DISCUSSION SESSION WITH DRINKS RECEPTION 

Presenters of poster should stand by their boards 

18:00 - 21:00 

VG&M

Conference social event - SOLD OUT

The conference dinner will be held at the Victoria Galleries and Museum (separate ticketed event)

Day 3, Thursday 2 July

09:25 - 09:30

Lecture Theatre 2

09:30 - 10:30

Lecture Theatre 2

Session three: Hallmarks of cancer

Chair: Regine Schneider-Stock, Co-chair: Cathy Richards

• S3-01 Plenary: Ruby Huang, National Taiwan University, Taiwan - Functional assessment of epithelial-mesenchymal transition (EMT) in the chick chorioallantoic membrane (CAM) model
• S3-02 Invited ECR talk: Kerstin Hubner, University Erlangen, Germany - Spatial whole-transcriptomics of CAM ovografts reveals downregulation of MYC targets and cell cycle arrest at the tumor invasion front
• S3-03 Selected short talk: Raphaela Ranjan, University of Munich, Germany - Integrated Cell Line, Organoid, and CAM Models Reveal Tumor-Intrinsic Control of Stromal Remodeling and Functional Heterogeneity in PDAC
• S3-04 Selected short talk: Thomas Bauer, Medical University of Graz, Austria - Microplastics Shape Colorectal Cancer Growth, Morphology and Angiogenesis in a CAM Xenograft Model

10:30 - 11:00

Breakout area & Flex2

11:00 - 12:00

Lecture Theatre 2

Session four: Complex cancer models

Chair: Ruby Huang, Co-chair: Tobias Faehling

• S4-01 Plenary: Andrew Sikora, University of Texas M.D. Anderson Cancer Centre, USA - Leveraging CAM-based tumor models for preclinical evaluation of cellular immunotherapies N.B This session will be online.
• S4-02 Invited short talk: Joe Sacco, University of Liverpool, UK - Developing co-culture CAM models to interrogate cell interactions in the metastatic niche and improve assessment of novel therapeutics in uveal melanoma
• S4-03 Selected short talk: Shari Aerts, Ghent University, Belgium - Species-specific mitochondrial DNA digital PCR improves the quantification of human and canine metastatic burden in CAM xenograft models
• S4-04 Selected short talk: Georg Fleugen, Heinrich-Heine-University and UniversityHospital Duesseldorf, Germany - Detection and Isolation of Spontaneous Human Circulating Gastrointestinal Tumor Cells in the In Vivo CAM Model

12:00 - 13:00

Breakout area & Flex2

13:00 - 14:00

Lecture Theatre 2

Session five: Pre-clinical applications

Chair: Yan Wang, Co-chair: Albert Odunlade

• S5-01 Plenary: Yan Wang, Inovotion, France - Chicken CAM assay: An alternative, 3Rs-compliant, in vivo model for the development of immunotherapy for cancer
• S5-02 Invited short talk: Miltiadis Fiorentzis, University Hospital Schleswig-Holstein, Germany - An Optimized Multimodal Uveal Melanoma CAM Platform for Longitudinal Imaging and Preclinical Therapy Evaluation
• S5-03 Selected short talk: Izabela Cymer, RCSI, Ireland - Detection and Isolation of Spontaneous Human Circulating Gastrointestinal Tumor Cells in the In Vivo CAM Model
• S5-04 Selected short talk: Mirja Gerbach, University Cancer Centre Hamburg, Germany - Establishing the CAM model for radiobiological investigations

14:00 - 15:00

Lecture Theatre 2

Session six: Biomolecular imaging

Chair: Rainer Schindl, Co-chair: Victor Martins

• S6-01 Plenary: Tim Whitney, King’s College London, UK - PET imaging of tumour response to therapy using the CAM
• S6-02 Invited ECR talk: Sandra Krause, Institute of Neuroscience and Medicine, Forschungszentrum Jülich, Germany - Role of positron emission tomography (PET), magnetic resonance imaging (MRI), and ex vivo autoradiography for translational orthotopic brain tumour imaging
• S6-03 Selected short talk: Karl McCullagh, University of Galway, Ireland - Novel method to measure the impact of VEGF on haemodynamics in the microcirculation of the CAM using Hoechst-labelled erythrocytes and intravital microscopy
• S6-04 Selected short talk: Jan Schulze, University of Liverpool, UK - BAP1-stratified arginine deprivation therapy in epithelioid pleural mesothelioma: preclinical PET/CT validation using an in ovo PDX Model

15:00 - 15:30

Breakout area & Flex2

COFFEE BREAK

Posters can be taken down during this break. 

15:30 - 16:30

Teaching Room 4

Workshop 3: Barriers to uptake of the CAM model

Coordinators: Judy Coulson and Sarah Barnett

• Interactive workshop

16:30 - 16:55

Teaching Room 4

SUMMARY, PRIZES AND DEPART

Copper–decavanadate complexes promote vascular remodeling and extracellular matrix maturation in the chorioallantoic membrane model. Presented by Luiz Fernando Pereira, Pontifical Catholic University of Parana, Brazil. 

Bioengineering a Mucoadhesive Composite for Ocular Chemotherapy: Preliminary Validation in a Novel CAM Model of Conjunctival Melanoma. Presented by Nimeet Desai, University of Liverpool, UK. 

Irritation and angiogenic property evaluation of gallic acid-crosslinked collagen scaffolds using the chick chorioallantoic membrane model. Presented by Nattapak Darumas, University of Liverpool, UK. 

Dental biomaterials implanted on chick embryo chorioallantoic membrane: from microscopy to AI based IKOSA and QuPAth analysis. Presented by Anca Maria Cimpean, Victor Babes University of Medicine and Pharmacy Timisoara, Romania. 

Electrospun PLGA fibers with copper tricalcium phosphate nanoparticles to increase angiogenesis in bone tissue engineering – with copper incorporated in nanoparticles like in a “Trojan horse”. Presented by Johanna Buschmann, University Hospital Zurich, Switzerland. 

Angiogenic footprint of human stem cell-based secretome - assessment of vascular network complexity using chicken aortic ring assay. Presented by Petra Wolint, University Hospital Zurich, Switzerland. 

Using an Organotypic Embryonic Chick Femur Defect Model to Evaluate Alginate–Minocycline Hydrogels for Bone Regeneration. Presented by Victor Martin, REQUIMTE, Portugal. 

Ex ovo quail chorioallantoic membrane (CAM) assay as a novel approach for biomaterial assessment. Presented by Zuzana Demcisakova, University of Veterinary Medicine and Pharmacy in Košice, Slovakia. 

Chorioallantoic Membrane Assay as an Alternative In Vivo Model for Investigating Chemoresistance and Novel Therapeutic Strategies in Triple-Negative Breast Cancer. Presented by Miguel Angel Merlos Rodrigo, Mendel University in Brno, Czechia. 

Anthraquinone-Based Telomerase Inhibition Suppresses Lung Cancer Growth and Invasive Dynamics in 3D and In Vivo Models. Presented by Natalia Maciejewska, Gdansk University of Technology, Poland. 

Interrogating Hippo Pathway Dependencies in Pleural Mesothelioma to Uncover Novel Therapeutic Opportunities: From in vitro to in ovo. Presented by Laura Bowker, University of Liverpool, UK. 

Tumour-Driven Angiogenesis in the Chorioallantoic Membrane Model: A Systematic Review and Meta-Analysis of Experimental Consistency. Presented by Xuejiao Xian, University of Bonn and University Hospital Bonn, Germany. 

Spatial Transcriptomic (ST) Profiling of Acetylation-Deficient SNAI1-Driven EMT in High Grade Serous Ovarian Carcinoma (HGSOC) Using a CAM Xenograft Model. Presented by Ifrah Taqdees, National Taiwan University, Taiwan. 

Investigating Adhesion-Adjacent Mechanotransduction in Oral Squamous Cell Carcinoma via YAP/TAZ using a CAM Xenograft Model. Presented by Cathy Richards, Royal College of Surgeons of Ireland, Ireland. 

Preferential Translation of HIF-1α Drives Hypoxic Tumour Growth: Insights from the Chick Embryo CAM Xenograft Model. Presented by Adam Albanese, University of Liverpool, UK. 

Developing Personalised Brain Tumour Avatars Using Human Meningioma and Brain Metastases in the Chicken Chorioallantoic Membrane (CAM) Model. Presented by Valeriia Katiuzhynska, University of Liverpool, UK. 

Establishing Systemic Doxycycline Delivery in the CAM Model Enables Functional Interrogation of Ewing Sarcoma Fusion Oncogene Dependency. Presented by Tobias Faehling, German Cancer Research Center, Germany. 

Investigating a nanoparticle-based mRNA vaccination platform against multicellular solid tumor spheroids in the chorioallantoic membrane (CAM) model. Presented by Niklas Feuchter, University Hospital Erlangen, Germany. 

Establishment of a CAM Co-culture model to study uveal melanoma liver metastasis. Presented by Jayanti Jha, University of Liverpool, UK. 

Establishment and Characterisation of an Orthotopic Chick Embryo Xenograft Model for Paediatric Medulloblastoma. Presented by Lara Kliebe, Research Centre Juelich, Germany. 

Beyond the Burn: Investigation of Tissue Destruction and CTC Dissemination upon Thermoablation of Pancreatic Cancer on the CAM. Presented by Christian Ballmeyer, University Hospital and Medical Faculty of the Heinrich-Heine-University Duesseldorf, Germany. 

Development of 14-day, non-sentient in ovo human tumour xenograft models for cancer drug discovery and 3Rs pre-clinical in vivo proof-of-concept. Presented by Simon Allison, University of Huddersfield, UK. 

Magnetic targeting of SPION-functionalized CAR-T cells in vascularized 2D and 3D melanoma models on the chorioallantoic membrane (CAM). Presented by Lucas Carnell, SEON, University Clinic Erlangen, Germany. 

Evaluation of water hyacinth as a potential therapeutic agent in breast cancer model using female albino rats. Presented by Albert Odunlade, Yaba College of Technology, Nigeria. 

Functional anti-angiogenic evaluation of a mesoionic compound using integrated endothelial assays and tumor-induced angiogenesis in the CAM model. Presented by Selene Elifio Esposito, Pontifícia Universidade Católica do Paraná, Brazil. 

Beyond the mouse: 3R-guided alternative animal models transforming cancer research. Presented by Richard Arndt, DKFZ, Germany. 

Vitamin C Potentiates 5-FU Efficacy and Overcomes Chemoresistance in uL3-Deficient Colorectal Cancer: Evidence from the CAM Model. Presented by Camilla Danisi, University of Naples Federico II, Italy. 

Evaluation of mesoporous silica nanoparticles for targeted drug delivery in breast and lung cancer using the CAM model. Presented by Cornelia Wilson, Canterbury Christ Church University, UK. 

Hyperspectral imaging of patient-derived endometriotic microtissues integrated in the chorioallantoic membrane model. Presented by Mareike Gruber, University Hospital Tübingen, Germany. 

Evaluation of rAAV Vector Visualization and Delivery Strategies Using the Chick Chorioallantoic Membrane (CAM) Model. Presented by Julia Hurnikova, University of Veterinary Medicine and Pharmacy in Kosice, Slovakia. 

Validated AI-Based Quantification of Superficial Chorioallantoic Membrane Vasculature. Presented by Qin Pu, University Hospital Bonn, Germany. 

The ostrich chorioallantoic model for in vivo imaging of cancer xenografts using a human PET/CT scanner. Presented by Marta Pomraenke, Jena University Hospital, Germany. 

The 3D In Vivo CAM Model as a Platform to Study Molecular Transport in Complex Vascular Systems. Presented by Sandra Maurer, University of Regensburg, Germany. 

Establishing an MC-38-Luc CAM Model for Tumor Growth and Metastasis Monitoring. Presented by Marcela Vlcnovska, St. Anne’s University Hospital, Czechia.