2024
Georgana, I., Hosmillo, M., Jahun, A. S., Emmott, E., Sorgeloos, F., Cho, K. -O., & Goodfellow, I. G. (2024). Porcine Sapovirus Protease Controls the Innate Immune Response and Targets TBK1.. Viruses, 16(2), 247. doi:10.3390/v16020247DOI: 10.3390/v16020247
2023
Huffman, R. G., Leduc, A., Wichmann, C., Di Gioia, M., Borriello, F., Specht, H., . . . Slavov, N. (2023). Prioritized mass spectrometry increases the depth, sensitivity and data completeness of single-cell proteomics. NATURE METHODS. doi:10.1038/s41592-023-01830-1DOI: 10.1038/s41592-023-01830-1
Gatto, L., Aebersold, R., Cox, J., Demichev, V., Derks, J., Emmott, E., . . . Slavov, N. (2023). Initial recommendations for performing, benchmarking and reporting single-cell proteomics experiments. NATURE METHODS, 20(3), 375-386. doi:10.1038/s41592-023-01785-3DOI: 10.1038/s41592-023-01785-3
2022
Maher, R., & Emmott, E. (2022). Electrospray ionization: “We taught elephants to fly”. The Biochemist, 44(5), 5-8. doi:10.1042/bio_2022_127DOI: 10.1042/bio_2022_127
Smertina, E., Carroll, A. J., Boileau, J., Emmott, E., Jenckel, M., Vohra, H., . . . Frese, M. (2022). Lagovirus Non-structural Protein p23: A Putative Viroporin That Interacts With Heat Shock Proteins and Uses a Disulfide Bond for Dimerization.. Frontiers in microbiology, 13, 923256. doi:10.3389/fmicb.2022.923256DOI: 10.3389/fmicb.2022.923256
2021
Petelski, A. A., Emmott, E., Leduc, A., Huffman, R. G., Specht, H., Perlman, D. H., & Slavov, N. (2021). Multiplexed single-cell proteomics using SCoPE2.. Nature protocols, 16(12), 5398-5425. doi:10.1038/s41596-021-00616-zDOI: 10.1038/s41596-021-00616-z
Meyer, B., Chiaravalli, J., Gellenoncourt, S., Brownridge, P., Bryne, D. P., Daly, L. A., . . . Emmott, E. (2021). Characterising proteolysis during SARS-CoV-2 infection identifies viral cleavage sites and cellular targets with therapeutic potential. NATURE COMMUNICATIONS, 12(1). doi:10.1038/s41467-021-25796-wDOI: 10.1038/s41467-021-25796-w
Johnson, J., Harman, V. M., Franco, C., Emmott, E., Rockliffe, N., Sun, Y., . . . Beynon, R. J. (2021). Construction of a la carte QconCAT protein standards for multiplexed quantification of user-specified target proteins. BMC BIOLOGY, 19(1). doi:10.1186/s12915-021-01135-9DOI: 10.1186/s12915-021-01135-9
Sutherland, H., Conley, M. J., Emmott, E., Streetley, J., Goodfellow, I. G., & Bhella, D. (2021). The Cryo-EM Structure of Vesivirus 2117 Highlights Functional Variations in Entry Pathways for Viruses in Different Clades of the Vesivirus Genus. JOURNAL OF VIROLOGY, 95(13). doi:10.1128/JVI.00282-21DOI: 10.1128/JVI.00282-21
Multiplexed single-cell proteomics using SCoPE2 (Journal article)
Petelski, A., Emmott, E., Leduc, A., Huffman, G., Specht, H., Perlman, D., & Slavov, N. (2021). Multiplexed single-cell proteomics using SCoPE2. doi:10.1101/2021.03.12.435034DOI: 10.1101/2021.03.12.435034
The cryo-EM structure of vesivirus 2117 highlights functional variations in entry pathways for viruses in different clades of the Vesivirus genus (Journal article)
Sutherland, H., Conley, M., Emmott, E., Streetley, J., Goodfellow, I., & Bhella, D. (2021). The cryo-EM structure of vesivirus 2117 highlights functional variations in entry pathways for viruses in different clades of the Vesivirus genus. doi:10.1101/2021.02.05.429895DOI: 10.1101/2021.02.05.429895
Specht, H., Emmott, E., Petelski, A. A., Huffman, R. G., Perlman, D. H., Serra, M., . . . Slavov, N. (2021). Single-cell proteomic and transcriptomic analysis of macrophage heterogeneity using SCoPE2. GENOME BIOLOGY, 22(1). doi:10.1186/s13059-021-02267-5DOI: 10.1186/s13059-021-02267-5
2020
Soh, T. K., Davies, C. T. R., Muenzner, J., Hunter, L. M., Barrow, H. G., Connor, V., . . . Crump, C. M. (2020). Temporal Proteomic Analysis of Herpes Simplex Virus 1 Infection Reveals Cell-Surface Remodeling via pUL56-Mediated GOPC Degradation. CELL REPORTS, 33(1). doi:10.1016/j.celrep.2020.108235DOI: 10.1016/j.celrep.2020.108235
Struwe, W., Emmott, E., Bailey, M., Sharon, M., Sinz, A., Corrales, F. J., . . . Barran, P. (2020). The COVID-19 MS Coalition—accelerating diagnostics, prognostics, and treatment. The Lancet, 395(10239), 1761-1762. doi:10.1016/s0140-6736(20)31211-3DOI: 10.1016/s0140-6736(20)31211-3
2019
Hosmillo, M., Lu, J., McAllaster, M. R., Eaglesham, J. B., Wang, X., Emmott, E., . . . Goodfellow, I. G. (2019). Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation. ELIFE, 8. doi:10.7554/eLife.46681DOI: 10.7554/eLife.46681
Specht, H., Emmott, E., Petelski, A., Huffman, G., Perlman, D., Serra, M., . . . Slavov, N. (2019). Single-cell proteomic and transcriptomic analysis of macrophage heterogeneity. doi:10.1101/665307DOI: 10.1101/665307
Emmott, E., de Rougemont, A., Hosmillo, M., Lu, J., Fitzmaurice, T., Haas, J., & Goodfellow, I. (2019). Polyprotein processing and intermolecular interactions within the viral replication complex spatially and temporally control norovirus protease activity. JOURNAL OF BIOLOGICAL CHEMISTRY, 294(11), 4259-4271. doi:10.1074/jbc.RA118.006780DOI: 10.1074/jbc.RA118.006780
Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation (Journal article)
Hosmillo, M., Lu, J., McAllaster, M., Eaglesham, J., Wang, X., Emmott, E., . . . Goodfellow, I. (2019). Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation. doi:10.1101/571455DOI: 10.1101/571455
On the value of preprints: An early career researcher perspective (Journal article)
Sarabipour, S., Debat, H. J., Emmott, E., Burgess, S. J., Schwessinger, B., & Hensel, Z. (2019). On the value of preprints: An early career researcher perspective. PLOS BIOLOGY, 17(2). doi:10.1371/journal.pbio.3000151DOI: 10.1371/journal.pbio.3000151
Ribosome Stoichiometry: From Form to Function (Journal article)
Emmott, E., Jovanovic, M., & Slavov, N. (2019). Ribosome Stoichiometry: From Form to Function. TRENDS IN BIOCHEMICAL SCIENCES, 44(2), 95-109. doi:10.1016/j.tibs.2018.10.009DOI: 10.1016/j.tibs.2018.10.009
Ifit1 regulates norovirus infection and enhances the interferon response in murine macrophage-like cells (Journal article)
Mears, H., Emmott, E., Chaudhry, Y., Hosmillo, M., Goodfellow, I., & Sweeney, T. (2019). Ifit1 regulates norovirus infection and enhances the interferon response in murine macrophage-like cells. doi:10.12688/wellcomeopenres.15223.1DOI: 10.12688/wellcomeopenres.15223.1
Mears, H. V., Emmott, E., Chaudhry, Y., Hosmillo, M., Goodfellow, I. G., & Sweeney, T. R. (2019). Ifit1 regulates norovirus infection and enhances the interferon response in murine macrophage-like cells.. Wellcome open research, 4, 82. doi:10.12688/wellcomeopenres.15223.1DOI: 10.12688/wellcomeopenres.15223.1
2018
Sarabipour, S., Debat, H. J., Emmott, E., Burgess, S., Schwessinger, B., & Hensel, Z. (2018). On the value of preprints: an early career researcher perspective. doi:10.7287/peerj.preprints.27400DOI: 10.7287/peerj.preprints.27400
Sarabipour, S., Debat, H., Emmott, E., Burgess, S., Schwessinger, B., & Hensel, Z. (2018). On the value of preprints: an early career researcher perspective. doi:10.7287/peerj.preprints.27400v1DOI: 10.7287/peerj.preprints.27400v1
Emmott, E. P., Jovanovic, M., & Slavov, N. (2018). Ribosome stoichiometry: from form to function.. Trends in Biochemical Sciences. doi:10.1016/j.tibs.2018.10.009DOI: 10.1016/j.tibs.2018.10.009
UK circulating strains of human parainfluenza 3: an amplicon based next generation sequencing method and phylogenetic analysis (Journal article)
Smielewska, A., Emmott, E., Ranellou, K., Popay, A., Goodfellow, I., & Jalal, H. (n.d.). UK circulating strains of human parainfluenza 3: an amplicon based next generation sequencing method and phylogenetic analysis. Wellcome Open Research, 3, 118. doi:10.12688/wellcomeopenres.14730.1DOI: 10.12688/wellcomeopenres.14730.1
Automated sample preparation for high-throughput single-cell proteomics (Journal article)
Specht, H., Harmange, G., Perlman, D., Emmott, E., Niziolek, Z., Budnik, B., & Slavov, N. (2018). Automated sample preparation for high-throughput single-cell proteomics. doi:10.1101/399774DOI: 10.1101/399774
Maintaining confidence in the reporting of scientific outputs (Journal article)
Sarabipour, S., Wissink, E., Burgess, S., Hensel, Z., Debat, H., Emmott, E., . . . Schwessinger, B. (2018). Maintaining confidence in the reporting of scientific outputs. doi:10.7287/peerj.preprints.27098v1DOI: 10.7287/peerj.preprints.27098v1
Sarabipour, S., Wissink, E. M., Burgess, S. J., Hensel, Z., Debat, H., Emmott, E., . . . Schwessinger, B. (2018). Maintaining confidence in the reporting of scientific outputs. doi:10.7287/peerj.preprints.27098DOI: 10.7287/peerj.preprints.27098
Ribosome stoichiometry: from form to function. (Journal article)
Emmott, E., Jovanovic, M., & Slavov, N. (2018). Ribosome stoichiometry: from form to function.. doi:10.7287/peerj.preprints.26991v1DOI: 10.7287/peerj.preprints.26991v1
UK circulating strains of human parainfluenza 3: an amplicon based next generation sequencing method and phylogenetic analysis (Journal article)
Smielewska, A., Emmott, E., Ranellou, K., Popay, A., Goodfellow, I., & Jalal, H. (2018). UK circulating strains of human parainfluenza 3: an amplicon based next generation sequencing method and phylogenetic analysis. doi:10.12688/wellcomeopenres.14730.2DOI: 10.12688/wellcomeopenres.14730.2
Smielewska, A., Emmott, E., Ranellou, K., Popay, A., Goodfellow, I., & Jalal, H. (2018). UK circulating strains of human parainfluenza 3: an amplicon based next generation sequencing method and phylogenetic analysis.. Wellcome open research, 3, 118. doi:10.12688/wellcomeopenres.14730.2DOI: 10.12688/wellcomeopenres.14730.2
Smielewska, A., Pearson, C., Popay, A., Roddick, I., Reacher, M., Emmott, E., . . . Jalal, H. (2018). Unrecognised Outbreak: Human parainfluenza virus infections in a pediatric oncology unit. A new diagnostic PCR and virus monitoring system may allow early detection of future outbreaks.. Wellcome open research, 3, 119. doi:10.12688/wellcomeopenres.14732.1DOI: 10.12688/wellcomeopenres.14732.1
2017
Phosphoproteomic Analysis Reveals the Importance of Kinase Regulation During Orbivirus Infection (Journal article)
Mohl, B. -P., Emmott, E., & Roy, P. (2017). Phosphoproteomic Analysis Reveals the Importance of Kinase Regulation During Orbivirus Infection. MOLECULAR & CELLULAR PROTEOMICS, 16(11), 1990-2005. doi:10.1074/mcp.M117.067355DOI: 10.1074/mcp.M117.067355
VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction (Journal article)
Hunter, M. R., Scourfield, E. J., Emmott, E., & Graham, S. C. (2017). VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction. BIOCHEMICAL JOURNAL, 474, 3615-3626. doi:10.1042/BCJ20170588DOI: 10.1042/BCJ20170588
Spatial and temporal control of norovirus protease activity is determined by polyprotein processing and intermolecular interactions within the viral replication complex (Journal article)
Emmott, E., de Rougemont, A., Haas, J., & Goodfellow, I. (2017). Spatial and temporal control of norovirus protease activity is determined by polyprotein processing and intermolecular interactions within the viral replication complex. doi:10.1101/175463DOI: 10.1101/175463
VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction (Journal article)
Hunter, M., Scourfield, E., Emmott, E., & Graham, S. (2017). VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction. doi:10.1101/169185DOI: 10.1101/169185
Norovirus-Mediated Modification of the Translational Landscape via Virus and Host-Induced Cleavage of Translation Initiation Factors (Journal article)
Emmott, E., Sorgeloos, F., Caddy, S. L., Vashist, S., Sosnovtsev, S., Lloyd, R., . . . Goodfellow, I. (2017). Norovirus-Mediated Modification of the Translational Landscape via Virus and Host-Induced Cleavage of Translation Initiation Factors. MOLECULAR & CELLULAR PROTEOMICS, 16(4), S215-S229. doi:10.1074/mcp.M116.062448DOI: 10.1074/mcp.M116.062448
Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures (Journal article)
Conley, M., Emmott, E., Orton, R., Taylor, D., Carneiro, D. G., Murata, K., . . . Bhella, D. (2017). Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures. JOURNAL OF GENERAL VIROLOGY, 98(1), 68-76. doi:10.1099/jgv.0.000658DOI: 10.1099/jgv.0.000658
2016
Norovirus-mediated modification of the translational landscape via virus and host-induced cleavage of translation initiation factors (Journal article)
Emmott, E., Sorgeloos, F., Caddy, S., Vashist, S., Sosnovtsev, S., Lloyd, R., . . . Goodfellow, I. (2016). Norovirus-mediated modification of the translational landscape via virus and host-induced cleavage of translation initiation factors. doi:10.1101/060772DOI: 10.1101/060772
2015
A Cell-based Fluorescence Resonance Energy Transfer (FRET) Sensor Reveals Inter-and Intragenogroup Variations in Norovirus Protease Activity and Polyprotein Cleavage (Journal article)
Emmott, E., Sweeney, T. R., & Goodfellow, I. (2015). A Cell-based Fluorescence Resonance Energy Transfer (FRET) Sensor Reveals Inter-and Intragenogroup Variations in Norovirus Protease Activity and Polyprotein Cleavage. JOURNAL OF BIOLOGICAL CHEMISTRY, 290(46), 27841-27853. doi:10.1074/jbc.M115.688234DOI: 10.1074/jbc.M115.688234
Evidence for Human Norovirus Infection of Dogs in the United Kingdom (Journal article)
Caddy, S. L., de Rougemont, A., Emmott, E., El-Attar, L., Mitchell, J. A., Hollinshead, M., . . . Goodfellow, I. (2015). Evidence for Human Norovirus Infection of Dogs in the United Kingdom. JOURNAL OF CLINICAL MICROBIOLOGY, 53(6), 1873-1883. doi:10.1128/JCM.02778-14DOI: 10.1128/JCM.02778-14
Murine Norovirus 1 (MNV1) Replication Induces Translational Control of the Host by Regulating eIF4E Activity during Infection (Journal article)
Royall, E., Doyle, N., Abdul-Wahab, A., Emmott, E., Morley, S. J., Goodfellow, I., . . . Locker, N. (2015). Murine Norovirus 1 (MNV1) Replication Induces Translational Control of the Host by Regulating eIF4E Activity during Infection. JOURNAL OF BIOLOGICAL CHEMISTRY, 290(8), 4748-4758. doi:10.1074/jbc.M114.602649DOI: 10.1074/jbc.M114.602649
2014
Norovirus Translation Requires an Interaction between the C Terminus of the Genome-linked Viral Protein VPg and Eukaryotic Translation Initiation Factor 4G (Journal article)
Chung, L., Bailey, D., Leen, E. N., Emmott, E. P., Chaudhry, Y., Roberts, L. O., . . . Goodfellow, I. G. (2014). Norovirus Translation Requires an Interaction between the C Terminus of the Genome-linked Viral Protein VPg and Eukaryotic Translation Initiation Factor 4G. Journal of Biological Chemistry, 289(31), 21738-21750. doi:10.1074/jbc.M114.550657DOI: 10.1074/jbc.M114.550657
Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics (Journal article)
Emmott, E., & Goodfellow, L. (2014). Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (89). doi:10.3791/51656DOI: 10.3791/51656
Murine norovirus: propagation, quantification, and genetic manipulation. (Journal article)
Hwang, S., Alhatlani, B., Arias, A., Caddy, S. L., Christodoulou, C., Cunha, J. B., . . . Wobus, C. E. (2014). Murine norovirus: propagation, quantification, and genetic manipulation.. Current protocols in microbiology, 33, 15K.2.1-15K.261. doi:10.1002/9780471729259.mc15k02s33DOI: 10.1002/9780471729259.mc15k02s33
2013
Progress towards the prevention and treatment of norovirus infections (Journal article)
Arias, A., Emmott, E., Vashist, S., & Goodfellow, I. (2013). Progress towards the prevention and treatment of norovirus infections. FUTURE MICROBIOLOGY, 8(11), 1475-1487. doi:10.2217/fmb.13.109DOI: 10.2217/fmb.13.109
The Cellular Interactome of the Coronavirus Infectious Bronchitis Virus Nucleocapsid Protein and Functional Implications for Virus Biology (Journal article)
Emmott, E., Munday, D., Bickerton, E., Britton, P., Rodgers, M. A., Whitehouse, A., . . . Hiscox, J. A. (2013). The Cellular Interactome of the Coronavirus Infectious Bronchitis Virus Nucleocapsid Protein and Functional Implications for Virus Biology. JOURNAL OF VIROLOGY, 87(17), 9486-9500. doi:10.1128/JVI.00321-13DOI: 10.1128/JVI.00321-13
Serological Evidence for Multiple Strains of Canine Norovirus in the UK Dog Population (Journal article)
Caddy, S., Emmott, E., El-Attar, L., Mitchell, J., de Rougemont, A., Brownlie, J., & Goodfellow, I. (2013). Serological Evidence for Multiple Strains of Canine Norovirus in the UK Dog Population. PLOS ONE, 8(12). doi:10.1371/journal.pone.0081596DOI: 10.1371/journal.pone.0081596
2012
Using SILAC and quantitative proteomics to investigate the interactions between viral and host proteomes (Journal article)
Munday, D. C., Surtees, R., Emmott, E., Dove, B. K., Digard, P., Barr, J. N., . . . Hiscox, J. A. (2012). Using SILAC and quantitative proteomics to investigate the interactions between viral and host proteomes. PROTEOMICS, 12(4-5), 666-672. doi:10.1002/pmic.201100488DOI: 10.1002/pmic.201100488
2011
Viruses and the Nucleolus (Journal article)
Matthews, D., Emmott, E., & Hiscox, J. (2011). Viruses and the Nucleolus. NUCLEOLUS, 15, 321-345. doi:10.1007/978-1-4614-0514-6_14DOI: 10.1007/978-1-4614-0514-6_14
2010
Quantitative Proteomic Analysis of A549 Cells Infected with Human Respiratory Syncytial Virus (Journal article)
Munday, D. C., Emmott, E., Surtees, R., Lardeau, C. -H., Wu, W., Duprex, W. P., . . . Hiscox, J. A. (2010). Quantitative Proteomic Analysis of A549 Cells Infected with Human Respiratory Syncytial Virus. MOLECULAR & CELLULAR PROTEOMICS, 9(11), 2438-2459. doi:10.1074/mcp.M110.001859DOI: 10.1074/mcp.M110.001859
Elucidation of the avian nucleolar proteome by quantitative proteomics using SILAC and changes in cells infected with the coronavirus infectious bronchitis virus (Journal article)
Emmott, E., Smith, C., Emmett, S. R., Dove, B. K., & Hiscox, J. A. (2010). Elucidation of the avian nucleolar proteome by quantitative proteomics using SILAC and changes in cells infected with the coronavirus infectious bronchitis virus. PROTEOMICS, 10(19), 3558-3562. doi:10.1002/pmic.201000139DOI: 10.1002/pmic.201000139
Quantitative Proteomics Using SILAC Coupled to LC-MS/MS Reveals Changes in the Nucleolar Proteome in Influenza A Virus-Infected Cells (Journal article)
Emmott, E., Wise, H., Loucaides, E. M., Matthews, D. A., Digard, P., & Hiscox, J. A. (2010). Quantitative Proteomics Using SILAC Coupled to LC-MS/MS Reveals Changes in the Nucleolar Proteome in Influenza A Virus-Infected Cells. JOURNAL OF PROTEOME RESEARCH, 9(10), 5335-5345. doi:10.1021/pr100593gDOI: 10.1021/pr100593g
Quantitative Proteomics Using Stable Isotope Labeling with Amino Acids in Cell Culture Reveals Changes in the Cytoplasmic, Nuclear, and Nucleolar Proteomes in Vero Cells Infected with the Coronavirus Infectious Bronchitis Virus (Journal article)
Emmott, E., Rodgers, M. A., Macdonald, A., McCrory, S., Ajuh, P., & Hiscox, J. A. (2010). Quantitative Proteomics Using Stable Isotope Labeling with Amino Acids in Cell Culture Reveals Changes in the Cytoplasmic, Nuclear, and Nucleolar Proteomes in Vero Cells Infected with the Coronavirus Infectious Bronchitis Virus. MOLECULAR & CELLULAR PROTEOMICS, 9(9), 1920-1936. doi:10.1074/mcp.M900345-MCP200DOI: 10.1074/mcp.M900345-MCP200
2009
Nucleolar targeting: the hub of the matter (Journal article)
Emmott, E., & Hiscox, J. A. (2009). Nucleolar targeting: the hub of the matter. EMBO REPORTS, 10(3), 231-238. doi:10.1038/embor.2009.14DOI: 10.1038/embor.2009.14
Adenovirus type 5 E4 Orf3 protein targets promyelocytic leukaemia (PML) protein nuclear domains for disruption via a sequence in PML isoform II that is predicted as a protein interaction site by bioinformatic analysis (Journal article)
Leppard, K. N., Emmott, E., Cortese, M. S., & Rich, T. (2009). Adenovirus type 5 E4 Orf3 protein targets promyelocytic leukaemia (PML) protein nuclear domains for disruption via a sequence in PML isoform II that is predicted as a protein interaction site by bioinformatic analysis. JOURNAL OF GENERAL VIROLOGY, 90, 95-104. doi:10.1099/vir.0.005512-0DOI: 10.1099/vir.0.005512-0
2008
Viral nucleolar localisation signals determine dynamic trafficking within the nucleolus (Journal article)
Emmott, E., Dove, B. K., Howell, G., Chappell, L. A., Reed, M. L., Boyne, J. R., . . . Hiscox, J. A. (2008). Viral nucleolar localisation signals determine dynamic trafficking within the nucleolus. VIROLOGY, 380(2), 191-202. doi:10.1016/j.virol.2008.05.032DOI: 10.1016/j.virol.2008.05.032
