Photo of Dr Edward Emmott

Dr Edward Emmott BSc (Hons), PhD

Tenure Track Fellow Biochemistry

Publications

Selected Publications

  1. High-throughput single-cell proteomics quantifies the emergence of macrophage heterogeneity (Journal article - 2019)
  2. Polyprotein processing and intermolecular interactions within the viral replication complex spatially and temporally control norovirus protease activity (Journal article - 2019)
  3. Automated sample preparation for high-throughput single-cell proteomics (Journal article - 2018)
  4. Ribosome Stoichiometry: From Form to Function (Journal article - 2019)
  5. Norovirus-Mediated Modification of the Translational Landscape via Virus and Host-Induced Cleavage of Translation Initiation Factors (Journal article - 2017)

2019

High-throughput single-cell proteomics quantifies the emergence of macrophage heterogeneity (Journal article)

Specht, H., Emmott, E., Perlman, D., Koller, A., & Slavov, N. (2019). High-throughput single-cell proteomics quantifies the emergence of macrophage heterogeneity. doi:10.1101/665307

DOI: 10.1101/665307

Polyprotein processing and intermolecular interactions within the viral replication complex spatially and temporally control norovirus protease activity (Journal article)

Emmott, E., de Rougemont, A., Hosmillo, M., Lu, J., Fitzmaurice, T., Haas, J., & Goodfellow, I. (2019). Polyprotein processing and intermolecular interactions within the viral replication complex spatially and temporally control norovirus protease activity. JOURNAL OF BIOLOGICAL CHEMISTRY, 294(11), 4259-4271. doi:10.1074/jbc.RA118.006780

DOI: 10.1074/jbc.RA118.006780

Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation (Journal article)

Hosmillo, M., Lu, J., McAllaster, M., Eaglesham, J., Wang, X., Emmott, E., . . . Goodfellow, I. (2019). Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation. doi:10.1101/571455

DOI: 10.1101/571455

On the value of preprints: An early career researcher perspective (Journal article)

Sarabipour, S., Debat, H. J., Emmott, E., Burgess, S. J., Schwessinger, B., & Hensel, Z. (2019). On the value of preprints: An early career researcher perspective. PLOS BIOLOGY, 17(2). doi:10.1371/journal.pbio.3000151

DOI: 10.1371/journal.pbio.3000151

Ribosome Stoichiometry: From Form to Function (Journal article)

Emmott, E., Jovanovic, M., & Slavov, N. (2019). Ribosome Stoichiometry: From Form to Function. TRENDS IN BIOCHEMICAL SCIENCES, 44(2), 95-109. doi:10.1016/j.tibs.2018.10.009

DOI: 10.1016/j.tibs.2018.10.009

Ifit1 regulates norovirus infection and enhances the interferon response in murine macrophage-like cells (Journal article)

Mears, H., Emmott, E., Chaudhry, Y., Hosmillo, M., Goodfellow, I., & Sweeney, T. (2019). Ifit1 regulates norovirus infection and enhances the interferon response in murine macrophage-like cells. doi:10.12688/wellcomeopenres.15223.1

DOI: 10.12688/wellcomeopenres.15223.1

Ifit1 regulates norovirus infection and enhances the interferon response in murine macrophage-like cells. (Journal article)

Mears, H. V., Emmott, E., Chaudhry, Y., Hosmillo, M., Goodfellow, I. G., & Sweeney, T. R. (2019). Ifit1 regulates norovirus infection and enhances the interferon response in murine macrophage-like cells.. Wellcome open research, 4, 82. doi:10.12688/wellcomeopenres.15223.1

DOI: 10.12688/wellcomeopenres.15223.1

2018

On the value of preprints: an early career researcher perspective (Journal article)

Sarabipour, S., Debat, H., Emmott, E., Burgess, S., Schwessinger, B., & Hensel, Z. (2018). On the value of preprints: an early career researcher perspective. doi:10.7287/peerj.preprints.27400v1

DOI: 10.7287/peerj.preprints.27400v1

On the value of preprints: an early career researcher perspective (Journal article)

Sarabipour, S., Debat, H. J., Emmott, E., Burgess, S., Schwessinger, B., & Hensel, Z. (n.d.). On the value of preprints: an early career researcher perspective. doi:10.7287/peerj.preprints.27400

DOI: 10.7287/peerj.preprints.27400

UK circulating strains of human parainfluenza 3: an amplicon based next generation sequencing method and phylogenetic analysis (Journal article)

Smielewska, A., Emmott, E., Ranellou, K., Popay, A., Goodfellow, I., & Jalal, H. (n.d.). UK circulating strains of human parainfluenza 3: an amplicon based next generation sequencing method and phylogenetic analysis. Wellcome Open Research, 3, 118. doi:10.12688/wellcomeopenres.14730.1

DOI: 10.12688/wellcomeopenres.14730.1

Automated sample preparation for high-throughput single-cell proteomics (Journal article)

Specht, H., Harmange, G., Perlman, D., Emmott, E., Niziolek, Z., Budnik, B., & Slavov, N. (2018). Automated sample preparation for high-throughput single-cell proteomics. doi:10.1101/399774

DOI: 10.1101/399774

Maintaining confidence in the reporting of scientific outputs (Journal article)

Sarabipour, S., Wissink, E., Burgess, S., Hensel, Z., Debat, H., Emmott, E., . . . Schwessinger, B. (2018). Maintaining confidence in the reporting of scientific outputs. doi:10.7287/peerj.preprints.27098v1

DOI: 10.7287/peerj.preprints.27098v1

Maintaining confidence in the reporting of scientific outputs (Journal article)

Sarabipour, S., Wissink, E. M., Burgess, S. J., Hensel, Z., Debat, H., Emmott, E., . . . Schwessinger, B. (n.d.). Maintaining confidence in the reporting of scientific outputs. doi:10.7287/peerj.preprints.27098

DOI: 10.7287/peerj.preprints.27098

Ribosome stoichiometry: from form to function. (Journal article)

Emmott, E. P., Jovanovic, M., & Slavov, N. (n.d.). Ribosome stoichiometry: from form to function.. doi:10.7287/peerj.preprints.26991

DOI: 10.7287/peerj.preprints.26991

Ribosome stoichiometry: from form to function. (Journal article)

Emmott, E., Jovanovic, M., & Slavov, N. (2018). Ribosome stoichiometry: from form to function.. doi:10.7287/peerj.preprints.26991v1

DOI: 10.7287/peerj.preprints.26991v1

UK circulating strains of human parainfluenza 3: an amplicon based next generation sequencing method and phylogenetic analysis (Journal article)

Smielewska, A., Emmott, E., Ranellou, K., Popay, A., Goodfellow, I., & Jalal, H. (2018). UK circulating strains of human parainfluenza 3: an amplicon based next generation sequencing method and phylogenetic analysis. doi:10.12688/wellcomeopenres.14730.2

DOI: 10.12688/wellcomeopenres.14730.2

UK circulating strains of human parainfluenza 3: an amplicon based next generation sequencing method and phylogenetic analysis. (Journal article)

Smielewska, A., Emmott, E., Ranellou, K., Popay, A., Goodfellow, I., & Jalal, H. (2018). UK circulating strains of human parainfluenza 3: an amplicon based next generation sequencing method and phylogenetic analysis. . Wellcome open research, 3, 118. doi:10.12688/wellcomeopenres.14730.2

DOI: 10.12688/wellcomeopenres.14730.2

Unrecognised Outbreak: Human parainfluenza virus infections in a pediatric oncology unit.  A new diagnostic PCR and virus monitoring system may allow early detection of future outbreaks. (Journal article)

Smielewska, A., Pearson, C., Popay, A., Roddick, I., Reacher, M., Emmott, E., . . . Jalal, H. (2018). Unrecognised Outbreak: Human parainfluenza virus infections in a pediatric oncology unit.  A new diagnostic PCR and virus monitoring system may allow early detection of future outbreaks.. Wellcome open research, 3, 119. doi:10.12688/wellcomeopenres.14732.1

DOI: 10.12688/wellcomeopenres.14732.1

2017

Phosphoproteomic Analysis Reveals the Importance of Kinase Regulation During Orbivirus Infection (Journal article)

Mohl, B. -P., Emmott, E., & Roy, P. (2017). Phosphoproteomic Analysis Reveals the Importance of Kinase Regulation During Orbivirus Infection. MOLECULAR & CELLULAR PROTEOMICS, 16(11), 1990-2005. doi:10.1074/mcp.M117.067355

DOI: 10.1074/mcp.M117.067355

VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction (Journal article)

Hunter, M. R., Scourfield, E. J., Emmott, E., & Graham, S. C. (2017). VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction. BIOCHEMICAL JOURNAL, 474, 3615-3626. doi:10.1042/BCJ20170588

DOI: 10.1042/BCJ20170588

Spatial and temporal control of norovirus protease activity is determined by polyprotein processing and intermolecular interactions within the viral replication complex (Journal article)

Emmott, E., de Rougemont, A., Haas, J., & Goodfellow, I. (2017). Spatial and temporal control of norovirus protease activity is determined by polyprotein processing and intermolecular interactions within the viral replication complex. doi:10.1101/175463

DOI: 10.1101/175463

VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction (Journal article)

Hunter, M., Scourfield, E., Emmott, E., & Graham, S. (2017). VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction. doi:10.1101/169185

DOI: 10.1101/169185

Norovirus-Mediated Modification of the Translational Landscape via Virus and Host-Induced Cleavage of Translation Initiation Factors (Journal article)

Emmott, E., Sorgeloos, F., Caddy, S. L., Vashist, S., Sosnovtsev, S., Lloyd, R., . . . Goodfellow, I. (2017). Norovirus-Mediated Modification of the Translational Landscape via Virus and Host-Induced Cleavage of Translation Initiation Factors. MOLECULAR & CELLULAR PROTEOMICS, 16(4), S215-S229. doi:10.1074/mcp.M116.062448

DOI: 10.1074/mcp.M116.062448

Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures (Journal article)

Conley, M., Emmott, E., Orton, R., Taylor, D., Carneiro, D. G., Murata, K., . . . Bhella, D. (2017). Vesivirus 2117 capsids more closely resemble sapovirus and lagovirus particles than other known vesivirus structures. JOURNAL OF GENERAL VIROLOGY, 98(1), 68-76. doi:10.1099/jgv.0.000658

DOI: 10.1099/jgv.0.000658

2016

Norovirus-mediated modification of the translational landscape via virus and host-induced cleavage of translation initiation factors (Journal article)

Emmott, E., Sorgeloos, F., Caddy, S., Vashist, S., Sosnovtsev, S., Lloyd, R., . . . Goodfellow, I. (2016). Norovirus-mediated modification of the translational landscape via virus and host-induced cleavage of translation initiation factors. doi:10.1101/060772

DOI: 10.1101/060772

2015

A Cell-based Fluorescence Resonance Energy Transfer (FRET) Sensor Reveals Inter-and Intragenogroup Variations in Norovirus Protease Activity and Polyprotein Cleavage (Journal article)

Emmott, E., Sweeney, T. R., & Goodfellow, I. (2015). A Cell-based Fluorescence Resonance Energy Transfer (FRET) Sensor Reveals Inter-and Intragenogroup Variations in Norovirus Protease Activity and Polyprotein Cleavage. JOURNAL OF BIOLOGICAL CHEMISTRY, 290(46), 27841-27853. doi:10.1074/jbc.M115.688234

DOI: 10.1074/jbc.M115.688234

Evidence for Human Norovirus Infection of Dogs in the United Kingdom (Journal article)

Caddy, S. L., de Rougemont, A., Emmott, E., El-Attar, L., Mitchell, J. A., Hollinshead, M., . . . Goodfellow, I. (2015). Evidence for Human Norovirus Infection of Dogs in the United Kingdom. JOURNAL OF CLINICAL MICROBIOLOGY, 53(6), 1873-1883. doi:10.1128/JCM.02778-14

DOI: 10.1128/JCM.02778-14

Murine Norovirus 1 (MNV1) Replication Induces Translational Control of the Host by Regulating eIF4E Activity during Infection (Journal article)

Royall, E., Doyle, N., Abdul-Wahab, A., Emmott, E., Morley, S. J., Goodfellow, I., . . . Locker, N. (2015). Murine Norovirus 1 (MNV1) Replication Induces Translational Control of the Host by Regulating eIF4E Activity during Infection. JOURNAL OF BIOLOGICAL CHEMISTRY, 290(8), 4748-4758. doi:10.1074/jbc.M114.602649

DOI: 10.1074/jbc.M114.602649

2014

Norovirus Translation Requires an Interaction between the C Terminus of the Genome-linked Viral Protein VPg and Eukaryotic Translation Initiation Factor 4G (Journal article)

Chung, L., Bailey, D., Leen, E. N., Emmott, E. P., Chaudhry, Y., Roberts, L. O., . . . Goodfellow, I. G. (2014). Norovirus Translation Requires an Interaction between the C Terminus of the Genome-linked Viral Protein VPg and Eukaryotic Translation Initiation Factor 4G. JOURNAL OF BIOLOGICAL CHEMISTRY, 289(31), 21738-21750. doi:10.1074/jbc.M114.550657

DOI: 10.1074/jbc.M114.550657

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics (Journal article)

Emmott, E., & Goodfellow, L. (2014). Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (89). doi:10.3791/51656

DOI: 10.3791/51656

Murine norovirus: propagation, quantification, and genetic manipulation. (Journal article)

Hwang, S., Alhatlani, B., Arias, A., Caddy, S. L., Christodoulou, C., Cunha, J. B., . . . Wobus, C. E. (2014). Murine norovirus: propagation, quantification, and genetic manipulation.. Current protocols in microbiology, 33, 15K.2.1-15K.261. doi:10.1002/9780471729259.mc15k02s33

DOI: 10.1002/9780471729259.mc15k02s33

2013

Progress towards the prevention and treatment of norovirus infections (Journal article)

Arias, A., Emmott, E., Vashist, S., & Goodfellow, I. (2013). Progress towards the prevention and treatment of norovirus infections. FUTURE MICROBIOLOGY, 8(11), 1475-1487. doi:10.2217/fmb.13.109

DOI: 10.2217/fmb.13.109

Serological Evidence for Multiple Strains of Canine Norovirus in the UK Dog Population (Journal article)

Caddy, S., Emmott, E., El-Attar, L., Mitchell, J., de Rougemont, A., Brownlie, J., & Goodfellow, I. (2013). Serological Evidence for Multiple Strains of Canine Norovirus in the UK Dog Population. PLOS ONE, 8(12). doi:10.1371/journal.pone.0081596

DOI: 10.1371/journal.pone.0081596

The Cellular Interactome of the Coronavirus Infectious Bronchitis Virus Nucleocapsid Protein and Functional Implications for Virus Biology (Journal article)

Emmott, E., Munday, D., Bickerton, E., Britton, P., Rodgers, M. A., Whitehouse, A., . . . Hiscox, J. A. (2013). The Cellular Interactome of the Coronavirus Infectious Bronchitis Virus Nucleocapsid Protein and Functional Implications for Virus Biology. JOURNAL OF VIROLOGY, 87(17), 9486-9500. doi:10.1128/JVI.00321-13

DOI: 10.1128/JVI.00321-13

2012

Using SILAC and quantitative proteomics to investigate the interactions between viral and host proteomes (Journal article)

Munday, D. C., Surtees, R., Emmott, E., Dove, B. K., Digard, P., Barr, J. N., . . . Hiscox, J. A. (2012). Using SILAC and quantitative proteomics to investigate the interactions between viral and host proteomes. PROTEOMICS, 12(4-5), 666-672. doi:10.1002/pmic.201100488

DOI: 10.1002/pmic.201100488

2010

Quantitative Proteomic Analysis of A549 Cells Infected with Human Respiratory Syncytial Virus (Journal article)

Munday, D. C., Emmott, E., Surtees, R., Lardeau, C. -H., Wu, W., Duprex, W. P., . . . Hiscox, J. A. (2010). Quantitative Proteomic Analysis of A549 Cells Infected with Human Respiratory Syncytial Virus. MOLECULAR & CELLULAR PROTEOMICS, 9(11), 2438-2459. doi:10.1074/mcp.M110.001859

DOI: 10.1074/mcp.M110.001859

Elucidation of the avian nucleolar proteome by quantitative proteomics using SILAC and changes in cells infected with the coronavirus infectious bronchitis virus (Journal article)

Emmott, E., Smith, C., Emmett, S. R., Dove, B. K., & Hiscox, J. A. (2010). Elucidation of the avian nucleolar proteome by quantitative proteomics using SILAC and changes in cells infected with the coronavirus infectious bronchitis virus. PROTEOMICS, 10(19), 3558-3562. doi:10.1002/pmic.201000139

DOI: 10.1002/pmic.201000139

Quantitative Proteomics Using SILAC Coupled to LC-MS/MS Reveals Changes in the Nucleolar Proteome in Influenza A Virus-Infected Cells (Journal article)

Emmott, E., Wise, H., Loucaides, E. M., Matthews, D. A., Digard, P., & Hiscox, J. A. (2010). Quantitative Proteomics Using SILAC Coupled to LC-MS/MS Reveals Changes in the Nucleolar Proteome in Influenza A Virus-Infected Cells. JOURNAL OF PROTEOME RESEARCH, 9(10), 5335-5345. doi:10.1021/pr100593g

DOI: 10.1021/pr100593g

Quantitative Proteomics Using Stable Isotope Labeling with Amino Acids in Cell Culture Reveals Changes in the Cytoplasmic, Nuclear, and Nucleolar Proteomes in Vero Cells Infected with the Coronavirus Infectious Bronchitis Virus (Journal article)

Emmott, E., Rodgers, M. A., Macdonald, A., McCrory, S., Ajuh, P., & Hiscox, J. A. (2010). Quantitative Proteomics Using Stable Isotope Labeling with Amino Acids in Cell Culture Reveals Changes in the Cytoplasmic, Nuclear, and Nucleolar Proteomes in Vero Cells Infected with the Coronavirus Infectious Bronchitis Virus. MOLECULAR & CELLULAR PROTEOMICS, 9(9), 1920-1936. doi:10.1074/mcp.M900345-MCP200

DOI: 10.1074/mcp.M900345-MCP200

2009

Adenovirus type 5 E4 Orf3 protein targets promyelocytic leukaemia (PML) protein nuclear domains for disruption via a sequence in PML isoform II that is predicted as a protein interaction site by bioinformatic analysis (Journal article)

Leppard, K. N., Emmott, E., Cortese, M. S., & Rich, T. (2009). Adenovirus type 5 E4 Orf3 protein targets promyelocytic leukaemia (PML) protein nuclear domains for disruption via a sequence in PML isoform II that is predicted as a protein interaction site by bioinformatic analysis. JOURNAL OF GENERAL VIROLOGY, 90, 95-104. doi:10.1099/vir.0.005512-0

DOI: 10.1099/vir.0.005512-0

Nucleolar targeting: the hub of the matter (Journal article)

Emmott, E., & Hiscox, J. A. (2009). Nucleolar targeting: the hub of the matter. EMBO REPORTS, 10(3), 231-238. doi:10.1038/embor.2009.14

DOI: 10.1038/embor.2009.14

2008

Viral nucleolar localisation signals determine dynamic trafficking within the nucleolus (Journal article)

Emmott, E., Dove, B. K., Howell, G., Chappell, L. A., Reed, M. L., Boyne, J. R., . . . Hiscox, J. A. (2008). Viral nucleolar localisation signals determine dynamic trafficking within the nucleolus. VIROLOGY, 380(2), 191-202. doi:10.1016/j.virol.2008.05.032

DOI: 10.1016/j.virol.2008.05.032