Our facilities

We have 11 mass spectrometers permitting a range of different protein and peptide-based analyses using a variety of different separation and ionisation processes. Different fragmentation capabilities, including high and low energy collision-induced dissociation (CID, HCD), electron transfer dissociation (ETD) and Ultraviolet Photodissociation (UVPD) offers enhanced capabilities for characterisation of proteins and their modifications.

Instruments

An image of the Thermo Scientific Q-Exactive at the Centre for Proteome Research

Thermo Scientific Q-Exactive
Thermo Scientific Q-Exactive HF
Thermo Scientific Orbitrap Fusion with FAIMSpro
Thermo Scientific Orbitrap Lumos with FAIMSpro
Thermo Scientific Exploris 480 with FAIMSpro
Waters Xevo TQ-S
Waters Synapt G2Si HDMS with ETD
Bruker TimsTOF HT
Bruker TimsTOF SCP

Online capabilities

Each instrument is capable of coupling to at least one of the online separation techniques or specialist ionisation sources listed below.

An image of the Thermo Scientific U3000 UPLC at the Centre for Proteome Research

Thermo Scientific U3000 UPLC
Waters NanoAcquity UPLC
SCIEX 8000+ capillary electrophoresis
Thermo Scientific HESI Probe
Waters REIMS Source
Waters MALDI Source
Waters DESI Source.
EvoSep

Offline capabilities

High pH Fractionation
Protein purification
De-Salting
Phosphopeptide enrichment
Opentron robotic system.

Non-mass spec capabilities

An image of protein sample equipment at the Centre for Proteome Research

Protein Simple. Milo, Single-cell Western Blot
Protein Simple. Wes, Capillary western blotting.