Beynon

Role of RNA polymerase III through proteomics

Roza Szatkowska, Manuel Garcia-Albornoz,*, Katarzyna Roszkowska, Stephen W. Holman, Emil Furmanek, Simon J. Hubbard, Robert J. Beynon and Malgorzata Adamczyk (2019) Glycolytic flux in Saccharomyces cerevisiae is dependent on RNA polymerase III and its negative regulator Maf1 [PUBMED] [PDF] [Abstract...]

Heparin binding proteome

Quentin M. Nunes, Dunhao Su, Philip J. Brownridge, Deborah M. Simpson, Changye Sun, Yong Li, Thao P. Bui, Xiaoying Zhang, Wei Huang, Daniel J. Rigden, Robert J. Beynon, Robert Sutton, David G. Fernig (2019) The heparin-binding proteome in normal pancreas and murine experimental acute pancreatitis [PUBMED] [PDF] [Abstract...]

Secretome analysis of cancer cells

J. Dinesh Kumar, Iman Aolymat, Laszlo Tiszlavicz, Zita Reisz, Hanan M. Garalla, Rob Beynon, Deborah Simpson, Graham J. Dockray and Andrea Varro (2019) Chemerin acts via CMKLR1 and GPR1 to stimulate migration and invasion of gastric cancer cells: putative role of decreased TIMP-1 and TIMP-2 [PUBMED] [PDF] [Abstract...]

Caught in a trap - proteomics of NETS

Elinor A. Chapman, Max Lyon, Deborah Simpson, David Mason, RobertJ.Beynon, RobertJ.Moots and HelenL.Wright (2019) Caught in a Trap? Proteomic Analysis of Neutrophil Extracellular Traps in Rheumatoid Arthritis and Systemic Lupus Erythematosus [PUBMED] [PDF] [Abstract...]

Using protemics to define MMP7 processing

Garalla HM, Lertkowit N, Tiszlavicz L, Reisz Z, Holmberg C, Beynon R, Simpson D, Varga A, Kumar JD, Dodd S, Pritchard DM, Moore AR, Rosztóczy AI, Wittman T, Simpson A, Dockray GJ, Varro A. (2018) Matrix metalloproteinase (MMP)-7 in Barrett's esophagus and esophageal adenocarcinoma: expression, metabolism, and functional significance. [PUBMED] [PDF] [Abstract...]

MUPs shape the volatile odours of mice

Roberts SA, Prescott MC, Davidson AJ, McLean L, Beynon RJ, Hurst JL. (2018) Individual odour signatures that mice learn are shaped by involatile major urinary proteins (MUPs). [PUBMED] [PDF] [Abstract...]

SIDLS: stable isotope labelling for secretome dynamics

Hammond DE, Kumar JD, Raymond L, Simpson DM, Beynon RJ, Dockray GJ, Varro A. (2018) Stable Isotope Dynamic Labeling of Secretomes (SIDLS) Identifies Authentic Secretory Proteins Released by Cancer and Stromal Cells. [PDF] [PUBMED] [Abstract...]

Proteomics in craniopharyngioma

John R. Apps · Gabriela Carreno · Jose Mario Gonzalez‐Meljem · Scott Haston · Romain Guiho ·Julie E. Cooper · Saba Manshaei · Nital Jani · Annett Hlsken · Benedetta Pettorini · Robert J. Beynon · Deborah M. Simpson · Helen C. Fraser · Ying Hong · Shirleen Hallang · Thomas J. Stone1 · Alex Virasami · Andrew M. Donson · David Jones · Kristian Aquilina · Helen Spoudeas1· Abhijit R. Joshi · Richard Grundy · Lisa C. D. Storer · Márta Korbonits · David A. Hilton · Kyoko Tossell · Selvam Thavaraj · Mark A. Ungless · Jesus Gil1 · Rolf Buslei · Todd Hankinson · Darren Hargrave · Colin Goding · Cynthia L. Andoniadou · Paul Brogan · Thomas S. Jacques · Hywel J. Williams · Juan Pedro Martinez‐Barbera (2018) Tumour compartment transcriptomics demonstrates the activation of in ammatory and odontogenic programmes in human adamantinomatous craniopharyngioma and identi es the MAPK/ERK pathway as a novel therapeutic target. [PUBMED] [PDF] [Abstract...]

Nextgen QconCATs - MEERCAT!

Takemori N, Takemori A, Tanaka Y, Endo Y, Hurst JL, Gómez-Baena G, Harman VM, Beynon RJ. (2017) MEERCAT: Multiplexed Efficient Cell Free Expression of Recombinant QconCATs For Large Scale Absolute Proteome Quantification. [PDF] [PUBMED] [Abstract...]

A new lipocalin, fully sequenced, from bank voles

Loxley GM, Unsworth J, Turton MJ, Jebb A, Lilley KS, Simpson DM, Rigden DJ, Hurst JL, Beynon RJ. (2017) Glareosin: a novel sexually dimorphic urinary lipocalin in the bank vole, Myodes glareolus. [PDF] [PUBMED]
[Abstract...]

New paper on CSF proteomics in paediatric meningitis

Guadalupe Gómez-Baena, Richard J. Bennett, Carmen Martínez-Rodríguez, Małgorzata Wnk, Gavin Laing, Graeme Hickey, Lynn McLean, Robert J. Beynon & Enitan D. Carrol (2017) Quantitative Proteomics of Cerebrospinal Fluid in Paediatric Pneumococcal Meningitis. [PDF] [PUBMED]

[Abstract...]

Proteomics suppporting supramolecular studies

M. Faulkner, J. Rodriguez-Ramos, G. F. Dykes, S. V. Owen, S. Casella, D. Simpson, R. Beynon and L. Liu (2017) Direct characterization of the native structure and mechanics of cyanobacterial carboxysomes [PUBMED] [PDF] [Abstract...]

Proteomics to identify galectin binding partners

Colomb F, Wang W, Simpson D, Zafar M, Beynon R, Rhodes JM, Yu LG. (2017) Galectin-3 interacts with the cell surface glycoprotein CD146 (MCAM, MUC18) and induces secretion of metastasis-promoting cytokines from vascular endothelial cells. [PDF] [PUBMED] [Abstract...]

Double standards in proteomics

Richard J. Bennett, Deborah M. Simpson, Stephen W. Holman, Sheila Ryan, Philip Brownridge, Claire E. Eyers, John Colyer, Robert J. Beynon (2017) DOSCATs: Double standards for protein quantification [PDF] [PUBMED] [Abstract...]

Proteomics of a marine organism

María del Carmen Muñoz-Marín, Guadalupe Gómez-Baena, Jesús Díez, Robert J. Beynon, David González-Ballester. Mikhail V. Zubkov and José M. García-Fernández (2017) Glucose Uptake in Prochlorococcus: Diversity of Kinetics and Effects on the Metabolism [PDF] [OA] [Abstract...]

Complete de novo sequencing of a mouse lemur protein

Jennifer Unsworth, Grace M. Loxley, Amanda Davidson, Jane L. Hurst, Guadalupe Gómez-Baena, Nicholas I. Mundy, Robert J. Beynon, Elke Zimmermann & Ute Radespiel. (2017) Characterisation of urinary WFDC12 in small nocturnal basal primates, mouse lemurs (Microcebus spp.) [PDF] [PUBMED] [Abstract...]

The joys of running a training course!

A short article, written for the Biochemist, explaining the role of the Society in training provision and our experiences of running the Quantitative Proteomics Course [PDF] [Abstract...]

Improving the quality of turnover measurement

Stephen W. Holman, Dean E. Hammond, Deborah M. Simpson, John Waters, Jane L. Hurst and Robert J. Beynon (2016) Protein turnover measurement using selected reaction monitoring-mass spectrometry (SRM MS). [PUBMED] [PDF] [Abstract...]

Proteome dynamics in a non-model species - the challenges!

Hammond DE, Claydon AJ, Simpson DM, Edward D, Stockley P, Hurst JL, Beynon RJ. (2016) Proteome Dynamics: Tissue Variation in the Kinetics of Proteostasis in Intact Animals. [PUBMED] [PDF] [Abstract...]

Proteomics of dystrophic muscle

Arecco N, Clarke CJ, Jones FK, Simpson DM, Mason D, Beynon RJ, Pisconti A. (2016) Elastase levels and activity are increased in dystrophic muscle and impair myoblast cell survival, proliferation and differentiation. [PDF] [PUBMED] [Abstract...]

Quantitative analysis of the yeast 'chaperome'

Rebecca J. Mackenzie, Craig Lawless [2], Stephen W. Holman, Karin Lanthaler, Robert J. Beynon, Chris M. Grant, Simon J. Hubbard and Claire E. Eyers (2106) Absolute protein quantification of the yeast chaperome under conditions of heat shock [PDF] [PUBMED] [Abstract...]

Proteomics of ocular cancer secretomes reveal malignancy potential

Angi, M., Kalirai, H, Prendegast, S., Simpson, D.M., Hammond, D.E., Madigan, M. C., Beynon, R.J. & Coupland, S.E. (2016) Proteomic analysis of uveal melanoma secretome reveals novel biological insights and potential biomarkers. [PDF] [PUBMED] [Abstract...]

Genetics of individual MUP variation

Sheehan MJ, Lee V, Corbett-Detig R, Bi K, Beynon RJ, Hurst JL, Nachman MW. (2016) Selection on Coding and Regulatory Variation Maintains Individuality in Major Urinary Protein Scent Marks in Wild Mice. [PUBMED] [PDF] [Abstract...]

QconCATs in equine wound healing

Bundgaard L, Bendixen E, Sørensen MA, Harman VM, Beynon RJ, Petersen LJ, Jacobsen S. (2016) A selected reaction monitoring based analysis of acute phase proteins in interstitial fluids from experimental equine wounds healing by secondary intention. [PUBMED] [PDF] [Abstract...]

The biggest absolute quantification study ever! A tale of 100 QconCATs for nearly 2,000 proteins

Lawless C, Holman SW, Brownridge P, Lanthaler K, Harman VM, Watkins R, Hammond DE, Miller RL, Sims PF, Grant CM, Eyers CE, Beynon RJ, Hubbard SJ. (2016) Direct and Absolute Quantification of over 1800 Yeast Proteins via Selected Reaction Monitoring. [Abstract...]

Sperm proteomes, gone large

Bayram HL, Claydon AJ, Brownridge PJ, Hurst JL, Mileham A, Stockley P, Beynon RJ, Hammond DE.(2016) Cross-species proteomics in analysis of mammalian sperm proteins. [PUBMED] [PDF] [Abstract...]

Of mice, mups and mass spectrometry

Robert J. Beynon, Stuart D. Armstrong, Amy J. Claydon, Amanda J. Davidson, Claire E. Eyers, James I. Langridge, Guadalupe Gómez-Baena, Victoria M. Harman, Jane L. Hurst, Victoria Lee, Lynn McLean, Rebecca Pattison, Sarah A. Roberts, Deborah M. Simpson, Jenny Unsworth, Matthias Vonderach, Jonathan Williams and Yvonne E. Woolerton (2015) Mass spectrometry for structural analysis and quantification of the Major Urinary Proteins of the house mouse. [PDF] [NotEvenIndexedInPubmed?] [Abstract...]

Proteomics and protein moonlighting

Beynon RJ, Hammond D, Harman V, Woolerton Y. (2014) The role of proteomics in studies of protein moonlighting. [PUBMED][PDF]
[Abstract...]

QconCAT compared to label-free for plasma proteins

Kramer G, Woolerton , van Straalen JP, Vissers JP, Dekker N, Langridge JI, Beynon RJ, Speijer D, Sturk A, Aerts JM. (2015) Accuracy and Reproducibility in Quantification of Plasma Protein Concentrations by Mass Spectrometry without the Use of Isotopic Standards. [PUBMED][PDF] [Abstract...]

Proteome plasticity in sperm competition

Ramm SA, Edward DA, Claydon AJ, Hammond DE, Brownridge, Hurst JL, Beynon RJ, Stockley P. (2015) Sperm competition risk drives plasticity in seminal fluid composition. [PUBMED] [PDF] [Abstract...]

Challenging the 'MHC' hypothesis

Green JP, Holmes AM, Davidson AJ, Paterson S, Stockley P, Beynon RJ, Hurst JL. (2015) The Genetic Basis of Kin Recognition in a Cooperatively Breeding Mammal. [PUBMED][PDF] [Abstract...]

Darcin stimulates neurogenesis

Hoffman, E., Pickavance, L., Thippeswamy, T., Beynon, R.J. & Hurst, J.L. (2015) The male sex pheromone darcin stimulates hippocampal neurogenesis and cell proliferation in the subventricular zone in female mice. [PUBMED] [PDF] [Abstract...]

QconCAT for equine acute phase proteins

Bundgaard L, Jacobsen S, Dyrlund TF, Sørensen MA, Harman VM, Beynon RJ, Brownridge PJ, Petersen LJ, Bendixen E. (2014) Development of a method for absolute quantification of equine acute phase proteins using concatenated peptide standards and selected reaction monitoring. [PUBMED][PDF]
[Abstract...]

Pheromones and contextual information.

Martín-Sanchez A, McLean L, Beynon RL, Hurst JL, Ayala G, Lanuza E, Martinez-Garcia F. (2015) . From sexual attraction to maternal aggression: When pheromones change their behavioural significance. [PUBMED][PDF] [Abstract...]

MUP complexity and analytical challenges

Beynon RJ, Armstrong SD, Gómez-Baena G, Lee V, Simpson D, Unsworth J, Hurst JL. (2014) The complexity of protein semiochemistry in mammals. [PUBMED][PDF] [Abstract...]

Sex pheromones are not aways attractive

Lanuza, E., Martin-Sanchez, A., Marco-Manclus, P., Cadiz-Moretti, B., Fortes-Marcw, L., Henandez-Martinez, A., Mclean, L., Beynon, R.J., Hurst, J.L. & Martinz-Garcia, F (2014) Sex pheromones are not always attractive: changes induced by learning and illness in mice. [PDF]
[Abstract...]

Darcin three dimensional structure solved!

Phelan, M.M., McLean, L., Armstrong, S.D., Hurst, J.L., Beynon, R.J. & Lian, L-Y. (2014) The structure, stability and pheromone binding of the male mouse protein sex pheromone darcin. [PUBMED][PDF] [Abstract...]

Structural comparison of central and peripheral mouse MUPs

Phelan MM, McLean L, Hurst JL, Beynon RJ, Lian LY. (2014) Comparative study of the molecular variation between 'central' and 'peripheral' MUPs and significance for behavioural signalling. [PUBMED][PDF]
[Abstract...]

Rats have MUPs too - but different!

Gómez-Baena G, Armstrong SD, Phelan MM, Hurst JL, Beynon RJ. (2014). The major urinary protein system in the rat. [PUBMED][PDF] [Abstract...]

Proteomics of myofibroblasts

Balabanova S, Holmberg C, Steele I, Ebrahimi B, Rainbow L, Burdyga T, McCaig C, Tiszlavicz L, Lertkowit N, Giger OT, Oliver S, Prior I, Dimaline R, Simpson D, Beynon R, Hegyi P, Wang TC, Dockray GJ, Varro A. (2014) The neuroendocrine phenotype of gastric myofibroblasts and its loss with cancer progression. [PDF] [PUBMED] [Abstract...]

Quantification of proteins in the human osteoarthritic secretome

Peffers MJ, Beynon RJ, Clegg PD. (2013) Absolute quantification of selected proteins in the human osteoarthritic secretome. [PDF][PUBMED] [Abstract...]

Quantitative proteomics in systems biology

Firczuk, H., Kannambath, S; Pahle, J; Claydon, Amy; Beynon, Robert, Duncan, John; Westerhoff, Hans; Mendes, Pedro & McCarthy, John (2013) An in vivo control map for the eukaryotic mRNA translation machinery. [PDF] [PUBMED] [Abstract...]

Romeo, Romeo, 'whiff'-fore art thou, Romeo?

Sarah A. Roberts, Amanda J. Davidson, Lynn McLean, Robert J. Beynon, Jane L. Hurst (2012) Pheromonal Induction of Spatial Learning in Mice. [PUBMED] [PDF] [Abstract...]

Who's got the biggest workload? Analysis of chaperone abundance and workload

Brownridge P, Lawless C, Payapilly AB, Lanthaler K, Holman SW, Harman VM, Grant CM, Beynon RJ, Hubbard SJ. Quantitative analysis of chaperone network throughput in budding yeast (2012). [PUBMED][PDF] [Abstract...]

A degrading synthesis of proteome turnover research!

Claydon, A.C. & Beynon, R.J. (2012) Proteome dynamics: revisiting turnover with a global perspective. [PUBMED][PDF] [Abstract...]

QconCATs for mastitis research

Bislev SL, Kusebauch U, Codrea MC, Beynon RJ, Harman VM, Røntved CM, Aebersold R, Moritz RL, Bendixen E. (2012) Quantotypic properties of QconCAT peptides targeting bovine host response to Streptococcus uberis. [PUBMED][PDF] [Abstract...]

Dynamic sperm labelling

Claydon AJ, Ramm SA, Pennington A, Hurst JL, Stockley P, Beynon R. (2012) Heterogenous turnover of sperm and seminal vesicle proteins in the mouse revealed by dynamic metabolic labeling. [PUBMED][PDF] [Abstract...]

Extending Progenesis to embrace SILAC and Hi3

Qi, D., Brownridge, P., Xia, D., Mackay, K., Gonzalez-Galarza, F.F., Kenyani, J., Harman, V., Beynon, R.J. & Jones, A.R. (2012) A software toolkit and interface for performing stable isotope labelling and top3 quantification using Progenesis LC-MS OMICS [PUBMED] [PDF] [Abstract...]

Designing QconCATs for a mammalian pathway

Simpson, D.M. & Beynon, R.J. (2012) QconCATs: design and expression of concatenated protein standards for multiplexed protein quantification. [PUBMED][PDF] [Abstract...]

Oxidative stress and MUPs

Garratt, M., McArdle, F., Stockely, P., Vasilaki, A., Beynon, R.J. Jackson, M.J. & Hurst, J.L. (2011) Tissue dependent changes in oxidative damage with male reproductive effort in house mice. [PDF] [Abstract...]

Whole animal protein turnover studies

Claydon, A.J., Thom, M.D., Hurst, J.L. & Beynon, R.J. (2011) Protein turnover: measurement of proteome dynamics by whole animal metabolic labelling with stable isotope labelled amino acids. Proteomics 12:1194-1206 [PUBMED][PDF] [Abstract...]

Asymetric equalisation with combinatorial beads

Jenny Rivers, Chris Hughes, Thérèse McKenna, Yvonne Woolerton, Johannes P. C. Vissers, James I. Langridge and Robert J. Beynon (2011) Asymmetric proteome equalization of the skeletal muscle proteome using a combinatorial hexapeptide library [PUBMED] [PDF]
[Abstract...]

Positional proteomics and MetAP-2 substrates

Thomas B. Sundberg, Nicole Darricarrere, Pasquale Cirone, Xia Li, Lucy McDonald, Xue Mei, Christopher J. Westlake, Diane C. Slusarski, Robert J. Beynon, and Craig M. Crews (2011) Disruption of Wnt Planar Cell Polarity Signaling by Aberrant Accumulation of the MetAP-2 Substrate Rab37. [PUBMED][PDF] [Abstract...]

Enzyme turnover and metabolic regulation

Arden, C, Petrie, J.L., Tudhope, S.J., Al-Oanzi, Z., Claydon, A.J., Beynon, R.J., Towle, H.C. & Agius, L. (2011)Elevated glucose represses liver glucokinase and induces its regulatory protein to safeguard hepatic phosphate homeostasis [PUBMED][PDF] [Abstract...]

Quantification of a metabolic pathway

Carroll KM, Simpson DM, Eyers CE, Knight CG, Brownridge P, Dunn W, Winder CL, Lanthaler K, Pir P, Malys N, Kell DB, Oliver SG, Gaskell SJ, Beynon RJ. (2011) Absolute quantification of a metabolic pathway in yeast: deployment of a complete QconCAT approach. [PUBMED][PDF] [Abstract...]

Scent of sensecence

Garratt, .M, Stockley, P., Armstrong, S.D., Beynon, R.J. & Hurst, J.L. (2011) The scent of senescence: sexual signalling and female preference in house mice. [PDF] [Abstract...]

Yeast complex proteomics

Drummond, S., Hildyard, J., Firczuk, H., Reamtong, O., Li, N.,Kannambath, S., Claydon, A., Beynon, R., Eyers, C.E. McCarthy, J.E.G (2011) Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes. [PUBMED][PDF] [Abstract...]

Schistosome parasite QconCAT

Castro-Borgesa, W., Simpson, D.M., Dowlea, A., Curwen, R.S., Thomas-Oates, J., Beynon, R.J. & Wilson, A. R. (2011) Abundance of tegument surface proteins in the human blood fluke Schistosoma mansoni determined by QconCAT proteomics. [PUBMED][PDF] [Abstract...]

Strategies for dynamic SILAC

Claydon, A & Beynon R (2011) Protein Turnover Methods in Single-Celled Organisms: Dynamic SILAC [PUBMED] [PDF] [Abstract...]

The end of proteomics

Davidson GR, Armstrong SD, Beynon R (2011) Positional proteomics at the N-terminus as a means of proteome simplification. [PUBMED][PDF]
[Abstract...]

Unkind cuts - proteolysis and proteomics

Brownridge, P. & Beynon, R.J. (2011) The importance of the digest: proteolysis and absolute quantification in proteomics. [PUBMED] [PDF] [Abstract...]

Global proteome quantification: dreams or reality?

Brownridge, P., Holman, S.W., Gaskell, S.J., Grant, C., Harman, V.M., Lanthaler, K., Lawless, C., O’cualain, R., Sims, P., Watkins, R & Beynon, R.J. (2011) Global absolute quantification of a proteome: Challenges in the deployment of a QconCAT strategy. Proteomics, 11, 2957-2970 [PUBMED][PDF] [Abstract...]

Limited proteolysis of native proteins

Ellison, D., Hinton, J., Hubbard, S.J. & Beynon, R.J. (1995) Limited proteolysis of native proteins: The interaction between avidin and proteinase K. Protein Science 4, 1337-1345 [PUBMED] [PDF] [Abstract...]

Molecular phenotypes in McArdle disease

Beynon, R.J., Bartram, C., Hopkins, P., Toescu, V., Gibson, H., Phoenix, J., & Edwards, RHT (1995) McArdle's disease: Molecular genetics and metabolic consequences of the phenotype. Muscle and Nerve 18, S18-S22 [PUBMED] [PDF] [Abstract...]

Review of McArdle disease

Bartram, C., Edwards, R.H.T. & Beynon, R.J. (1995) McArdle's disease-muscle glycogen phosphorylase deficiency Biochimica et Biophysica Acta 1272, 1-13 [PUBMED] [PDF] [Abstract...]

MMP zymography

Coughlan, A. D., Carter, S. D., Robertson, D. H. L., Kelly, D. F., Bennett, D., May, C. & Beynon, R. J. (1995). Zymographic analysis of synovial fluid metalloproteinases in canine arthritis. Vet. Comp. Orthopaed. Traum. 8, 62. [PUBMED] [PDF] [Abstract...]

MUP molecular heterogeneity

Robertson, D.H.L., Gaskell, S.J., Cox, K., Evershed, R.P. & Beynon, R.J. Molecular heterogeneity in the Major Urinary Proteins of the house mouse, Mus musculus (1996) Biochem. J. 316, 265-272 [PUBMED] [PDF] [Abstract...]

Soluble urinary meprin

Beynon, RJ, Oliver, S. & Robertson, D.H.L. (1996) Characterisation of the soluble secreted form of mouse meprin Biochem. J. 315, 461-466 [PUBMED] [PDF] [Abstract...]

PLP and glycogen phosphorylase

Beynon RJ, Bartram C, Flannery A, Evershed RP, Leyland D, Hopkins P, Toescu V, Phoenix J, Edwards RH. (1996) Interrelationships between metabolism of glycogen phosphorylase and pyridoxal phosphate--implications in McArdle's disease. Adv Food Nutr Res. 1996;40:135-47. [PUBMED] [PDF] [Abstract...]

Cold induction of desaturases, how carp cope with the cold

Tiku, P.E., Gracey, A.Y., Macartney, A.I., Beynon, R.J. & Cossins, A.R. (1996) Cold-induced expression of delta-9 desaturase in carp by transcriptional and posttranslational mechanisms. Science 271, 815-863 [PUBMED] [PDF] [Abstract...]

PLP to monitor phosphorylase turnover

Beynon, R.J., Leyland, D.M., Evershed, R.P., Edwards, R.H.T. & Coburn, S.P. (1996) Measurement of the turnover of glycogen phosphorylase by gas chromatography/mass spectrometry using stable isotope derivatives of pyridoxine (Vitamin B6) Biochem J 317, 613-619 [PUBMED] [PDF] [Abstract...]

Wild mice show much more MUP polymorphism than inbred lab mice

Robertson, D.H.L., Hurst, J L., Bolgar, M.S., Gaskell, S.J.& Beynon, R.J. (1997) Molecular heterogeneity of urinary proteins in wild house mouse populations. Rap. Comm. Mass Spec.11, 786-790 [PUBMED] [PDF] [Abstract...]

Limited proteolysis of native proteins

Hubbard, S.J., Beynon, R.J. & Thornton, J.M. (1998) Assessment of conformational parameters as predictors of limited proteolytic sites in native protein structures. Prot. Eng. 11, 349-359 [PUBMED] [PDF] [Abstract...]

MUPs can take up ligands from the environment

Robertson, D., Hurst, J.L., Hubbard, S.J., Gaskell, S.J. & Beynon, R.J. (1998) Ligands of urinary lipocalins from the mouse -uptake of environmentally-derived chemicals. J. Chem. Ecol. 24, 1127-1140 [PUBMED] [PDF] [Abstract...]

MUPs bind volatiles and extend the duration of odour signals

Hurst, J.L., Robertson, D.H.L., Tolladay, U. & Beynon, R.J. (1998) Proteins in scent marks of male house mice extend the emission of olfactory signals. Anim. Behav. 55, 1285-1297 [PUBMED] [PDF] [Abstract...]

Vitamin B6 and McArdle disease

Pheonix, J., Hopkins, P.G., Bartram, C., Beynon, R.J., Quinlivan, R.C.M. & Edwards, R.H.T. (1998) Effect of vitamin B6 supplementation in McArdle's disease: a strategic case study. Neuromuscular Disorders. 8, 210-212 [PUBMED] [PDF] [Abstract...]

MMP Zymography

Coughlan, A. R., Robertson, D. H. L., Burke, R., Beynon, R.J., Carter, S. D. (1998) Isolation and identification of canine matrix metalloproteinase-2 (MMP-2). Vet J. 155, 231-237 [PUBMED] [PDF] [Abstract...]

MMP zymography

Coughlan, A. R, Robertson, D. H. L, Bennett, D., May, C., Beynon, R.J., Carter, S.D. (1998) Matrix metalloproteinases 2 and 9 in canine rheumatoid arthritis. Vet. Record 143, 219-223 [PUBMED] [PDF] [Abstract...]

Contact with urinary proteins stimulates countermarking in male mice

Humphries, R. E., Robertson, D. H., Beynon, R. J. & Hurst, J. L. (1999). Unravelling the chemical basis of competitive scent marking in house mice. Anim Behav 58, 1177-1190. [PUBMED] [PDF] [Abstract...]

Limited proteolysis of MUPs

Wu, C., Robertson, D.H.L., Hubbard, S.J. Gaskell, S.J. & Beynon, R.J. (1999) Proteolysis of native enzymes: Trapping of a reaction intermediate. J. Biol. Chem. 274: 1108-1115 [PUBMED] [PDF] [Abstract...]

How to suppress unwanted proteolyis

Beynon, R.J & Oliver, S. (1996) Prevention of unwanted proteolysis, in "Protein Purification Protocols" (Doonan, S., ed) Humana Press. pp 81-102 [Abstract...]

Review of meprins

Gorbea, C.M., Beynon, R.J. & Bond, J.S. (1994) Meprins: activation, subunit interactions and anchoring. in "Mammalian Brush Border membrane proteins II" (eds Lentze, M.J., Naim, H.Y. & Grand, R.J.) pp89-97 Thieme, Stuttgart [Abstract...]

Review of astacins

Bond, J.S. & Beynon, R.J.(1995) The astacin family of metalloendopeptidases. Protein Science 4,1247-1261 [PUBMED] [PDF] [Abstract...]

PLP, glycogen phosphorylase and McArdle disease

Beynon, R.J. Bartram, C., Flannery, A.V. Evershed, R.P., Leyland, D.M., Hopkins, P., Toescu, V., Phoenix, J. & Edwards, R.H.T. (1996) Interrelationships between metabolism and glycogen phosphorylase and pyridoxal phosphate-implications in McArdle's disease. Food & Nutrition 40, 135-147 [PUBMED] [PDF] [Abstract...]

Soluble meprin alpha

Beynon, R.J., Oliver, S., Stateva, L., Evershed, R.P. & Robertson, D.H.L. (1997) Soluble forms of kidney meprin alpha. in 'The Astacins' (R Zwilling & W Stocker, eds) Verlag DR Kovac, Hamburg, 1997 [Abstract...]

Review of thermolysins

Beynon, R.J. & Beaumont, A. (1998) M4 proteases: thermolysins. in "Handbook of Proteolytic Enzymes" (Barrett, A.J. Rawlings, N. & Woessner, F., eds) Academic press 1037-1046 [Abstract...]

Review of M4 proteases

Beynon, R.J. & Beaumont, A. (1998) M4 proteases: bacillolysins. in "Handbook of Proteolytic Enzymes" (Barrett, A.J. Rawlings, N. & Woessner, F., eds) Academic press 1046-1050 [Abstract...]

Protein binding in chemical communication

Beynon, R.J., Robertson, D.H.L., Hubbard, S. J., Gaskell, S.J. & Hurst, J.L. (1999) The role of protein binding in chemical communication: major urinary proteins in the house mouse. in "Chemical Signals in Vertebrates" (R.E. Johnston, D. Müller-Schwarze and P.W.Sorensen eds) Plenum Press pp 137-147 [Abstract...]

Many MUPs expressed by wild mice

Pes, D., Robertson, D.H.L., Hurst, J.L.., Gaskell, S.J. & Beynon, R.J. (1999) How many Major Urinary Proteins are produced by the house mouse Mus domesticus? in "Chemical Signals in Vertebrates" (R.E. Johnston, D. Müller-Schwarze and P.W.Sorensen eds) Plenum Press 149-161 [Abstract...]

Inbreeding compromises competitor recognition

Nevison, C.M., Barnard, C.J., Beynon, R.J. & Hurst, J.L. (2000) The consequences of inbreeding for recognizing competitors. Proceedings of the Royal Society 267, 687-694. [PUBMED] [PDF] [Abstract...]

Canine MMP identification

Coughlan AR, Robertson DH, Burke R, Beynon RJ, Carter SD. (2000) Isolation and identification of canine matrix metalloproteinase-2 (MMP-2). Vet J. 155:231-237 [PUBMED] [PDF] [Abstract...]

Overview of McArdle disease

Quinlivan, R. & Beynon, R.J. (2000) McArdle's disease: an overview. BIMDG Bulletin, 18, 40-46 [PUBMED] [PDF] [Abstract...]

Individual recognition through MUPs

Hurst, J. L., Payne, C. E., Nevison, C. M., Marie, A. D., Humphries, R. E., Robertson, D. H., Cavaggioni, A. & Beynon, R. J. (2001). Individual recognition in mice mediated by major urinary proteins. Nature 414, 631-634. [PUBMED] [PDF] [Abstract...]

Derivatization for proteomics

Brancia, F.L., Butt, A., Beynon, R.J., Hubbard, S.J., Gaskell, S.J. & Oliver, S.G. (2001) A combination of chemical derivatisation and improved bioinformatic tools optimises protein identification for proteomics. Electrophoresis, 22, 552-559 [PUBMED] [PDF] [Abstract...]

Chromatographic separation at the protein level

Butt, A., Davison, M.D., Smith, G.J., Young, J.A., Gaskell, S.J., Oliver, S.G. & Beynon, R.J. (2001) Chromatographic separations as a prelude to two-dimensional electrophoresis in proteomics analysis. Proteomics, 1, 42-53. [PUBMED] [PDF] [Abstract...]

MUP polymorphism & ligand binding

Marie,A.D., Veggerby, C, Robertson, D.H.L., Gaskell, S J., Hubbard, S.J., Martinsen, L., Hurst, J.L. & Beynon, R.J. (2001) The effect of protein polymorphisms on ligand binding by Major Urinary Proteins. Protein Science 10, 411-417 [PUBMED] [PDF] [Abstract...]

Myths about MUPs

Beynon, R.J., Hurst, J.L., Gaskell, S.J., Hubbard, S.J., Humphries, R.E., Malone, N., Marie, A.D., Martinsen, L., Nevison, C.M., Payne, C.E., Robertson, D.H.L. & Veggerby,C. (2001) Mice, MUPs and myths – structure-function relationships of the major urinary proteins. In: Chemical Signals in Vertebrates (Ed. A. Marchlewska-Koj, D. Muller-Schwarze & J. Lepri) pp149-156. New York: Plenum Press. [Abstract...]

Limited proteolysis

Hubbard, S. J. & Beynon, R. J. (2001). Proteolysis of native proteins as a structural probe. In Proteolytic Enzymes. A Practical Approach (Beynon, R. J. & Bond, J. S., eds.), pp. 233-264. OUP, Oxford. [Abstract...]

MUP ligand binding

Robertson, D.H.L., Marie, A.D., Veggerby, C., Hurst, J.L. & Beynon, R.J. (2001) Characteristics of ligand binding and release by major urinary proteins. . In: Chemical Signals in Vertebrates (Ed. A. Marchlewska-Koj, D. Muller-Schwarze & J. Lepri) pp169-176. Plenum Press, New York. [Abstract...]

MUP heterogeneity

Payne, C.E., Malone, N., Humphries, R., Bradbrook, C., Veggerby, C., Beynon, R.J. & Hurst, J.L. (2001) Heterogeneity of major urinary proteins in house mice: population and sex differences. . In: Chemical Signals in Vertebrates (Ed. A. Marchlewska-Koj, D. Muller-Schwarze & J. Lepri) pp233-240. Plenum Press, New York. [Abstract...]

Inbreeding and individual recognition

Nevison, C.M., Barnard, C.J., Beynon, R.J. & Hurst, J.L. (2001) Effects of inbreeding and social status on individual recognition in mice. . In: Chemical Signals in Vertebrates (Ed. A. Marchlewska-Koj, D. Muller-Schwarze & J. Lepri) pp225-232. Plenum Press, New York. [Abstract...]

Social status and mate choice

Malone, N., Payne, C.E., Beynon, R.J. & Hurst, J.L. (2001) Social status, odour communication and mate choice in wild house mice. . In: Chemical Signals in Vertebrates (Ed. A. Marchlewska-Koj, D. Muller-Schwarze & J. Lepri) pp217-224. Plenum Press, New York. [Abstract...]

MUPs and competitive scent marking

Humphries, R.E., Robertson, D.H.L., Nevison, C.M, Beynon, R.J. & Hurst, J.L. (2001) The role of urinary proteins and volatiles in competitive scent marking among male house mice. . In: Chemical Signals in Vertebrates (Ed. A. Marchlewska-Koj, D. Muller-Schwarze & J. Lepri) pp353-360. Plenum Press, New York. [Abstract...]

Scent signals of status

Hurst, J.L., Beynon, R.J., Humphries, R.E., Malone, N., Nevison, C.M., Payne, C.E., Robertson, D.H.L. & Veggerby, C. (2001) Information in scent signals of status: the interface between behaviour and chemistry. . In: Chemical Signals in Vertebrates (Ed. A. Marchlewska-Koj, D. Muller-Schwarze & J. Lepri) pp43-50. Plenum Press, New York. [Abstract...]

Protease inhibitors

Beynon, R. J. & Salvesen, G. (2001). Commercially available proteinase inhibitors. In Proteolytic Enzymes. A Practical Approach (Beynon, R. J. & Bond, J. S., eds.), pp. 317-330. OUP, Oxford. [Abstract...]

MUP polymorphic variants

Veggerby, C., Payne, C.E., Gaskell, S.J., Robertson, D.H.L., Hurst, J.L. & Beynon, R.J. (2001) Polymorphic variants of mouse majory urinary proteins. . In: Chemical Signals in Vertebrates (Ed. A. Marchlewska-Koj, D. Muller-Schwarze & J. Lepri) pp157-168. Plenum Press, New York. [Abstract...]

MUP polymorphism in an island population of mice

Beynon, R. J., Veggerby, C., Payne, C. E., Robertson, D. H., Gaskell, S. J., Humphries, R. E. & Hurst, J. L. (2002). Polymorphism in major urinary proteins: molecular heterogeneity in a wild mouse population. J Chem Ecol 28, 1429-46. [PUBMED] [PDF] [Abstract...]

First paper on proteome turnover

Pratt, J. M., Petty, J., Riba-Garcia, I., Robertson, D. H., Gaskell, S. J., Oliver, S. G. & Beynon, R. J. (2002). Dynamics of protein turnover, a missing dimension in proteomics. Mol Cell Proteomics 1, 579-91. [PUBMED] [PDF] [Abstract...]

Stable isotope labelling for identification

Pratt, J. M., Robertson, D. H., Gaskell, S. J., Riba-Garcia, I., Hubbard, S. J., Sidhu, K., Oliver, S. G., Butler, P., Hayes, A., Petty, J. & Beynon, R. J. (2002). Stable isotope labelling in vivo as an aid to protein identification in peptide mass fingerprinting. Proteomics 2, 157-63. [PUBMED] [PDF] [Abstract...]

McArdle disease

Beynon, R. J., Quinlivan, R. C. & Sewry, C. A. (2002). Selected disorders of carbohydrate metabolism. In Structural and Molecular Basis of Skeletal Muscle Diseases (Karpati, G., ed.), pp. 182-188. ISN Neuropathology Press, Basle. [PUBMED] [PDF]  [Abstract...]

Intact mass proteomics

Hayter J.R., Robertson, D.H.L., Gaskell, S.J. & Beynon, R.J. (2003) Proteome analysis of intact proteins in complex mixtures. Mol. Cell. Proteomics 2, 85-95 [PUBMED] [PDF] [Abstract...]

Involatile ownership signals in mouse scent marks

Nevison, C.M., Armstrong, S.; Beynon, R.J. Humphries, R.E. & Hurst, J.L. (2003) The ownership signature in mouse scents marks is involatile. Proceedings of the Royal Society series B, 270, 1957-1963 [PUBMED] [PDF] [Abstract...]

McArdle disease

Beynon, R.J. & Quinlivan, R M. (2003) McArdle’s disease, Glycogenosis V. disease. In Encyclopaedic Reference of Molecular Mechanisms of Disease (Lang, F., ed) pp… [Abstract...]

Tarui disease review

Quinlivan, R M & Beynon, R.J. (2003) Tarui’s disease, Glycogenosis VII. In Encyclopaedic Reference of Molecular Mechanisms of Disease (Lang, F., ed) pp … [Abstract...]

MUPs play multiple roles in mouse communication

Beynon, R.J. & Hurst, J.L. (2003) Multiple roles of major urinary proteins in the house mouse, Mus musculus. Biochemical Journal Transactions, 31, 142-146 [PUBMED] [PDF] [Abstract...]

A proteomics workbench?

Beynon, R.J. (2004) Specification of a proteomics workbench. Comparative and Functional Genomics 5, 52-55 [PUBMED] [PDF] [Abstract...]

Sequential exoproteolysis is not valid as a structural probe

Beynon, R.J. (2004) Sequential exoproteolysis as a structural probe: a cautionary note. J. Mass. Spec. 39, 188-192. [PUBMED] [PDF] [Abstract...]

Proteomics of copper effects in Ronaldsay sheep

Simpson, D.M., Beynon, R.J., Robertson, D.H.L., Loughran, M.J. & Haywood, S. (2004) Copper associated liver disease: a proteomics study of copper challenge in a sheep model. Proteomics 4, 524-536. [PUBMED] [PDF] [Abstract...]

Review of rodent urinary lipocalins

Beynon, R.J. & Hurst, J.L. (2004) Urinary proteins and the modulation of chemical scents in mice and rats. Peptides 25(9):1553-1563 [PUBMED] [PDF] [Abstract...]

Proteomics of tissue samples

McLean, L., Doherty, M.K., Deeming, C.D. & Beynon, R.J. (2004) A proteome analysis of the subcutaneous gel in avian hatchlings. Mol. Cell. Proteomics 3, 250-256 [PUBMED] [PDF] [Abstract...]

Proteomics of growing muscle

Doherty, M.K., McLean, L., Hayter, J.R., Pratt, J.M,, Robertson, D.H.L., El-Sahfei, A.E., Gaskell, S.J. & Beynon, R.J. (2004) The proteome of chicken skeletal muscle: changes in soluble protein expression during growth in a layer strain. Proteomics 4, 2082-2093 [PUBMED] [PDF] [Abstract...]

Prevention of proteolysis

Beynon RJ, Oliver S. (2004) Avoidance of proteolysis in extracts. Methods Mol Biol. 244:75-84. [PUBMED] [PDF] [Abstract...]

Scent wars

Hurst, J.L. & Beynon, R.J. (2004) Scent Wars: The chemobiology of competitive signaling in mice. BioEssays 26, 1288-1298 [PUBMED] [PDF] [Abstract...]

Cochrane review of McArdle disease

Quinlivan R, Beynon RJ. Pharmacological and nutritional treatment for McArdle's disease (Glycogen Storage Disease type V). Cochrane Database Syst Rev. 2004;(3):CD003458. [PUBMED] [PDF] [Abstract...]

Protein turnover in a vertebrate

Doherty MK, McClean L, Edwards I, McCormack H, McTeir L, Whitehead C, Gaskell SJ, Beynon RJ. (2004) Protein turnover in chicken skeletal muscle: understanding protein dynamics on a proteome-wide scale. Br Poult Sci. 45 Suppl 1:S27-8 [PUBMED] [PDF] [Abstract...]

Metabolic labelling in proteomics

Beynon RJ, Pratt J.M. (2005) Metabolic labelling of proteins for proteomics. Mol Cell Proteomics. 4, 857-872 [PUBMED] [PDF] [Abstract...]

Proteome dynamics review

Beynon RJ.(2005) The dynamics of the proteome: strategies for measuring protein turnover on a proteome-wide scale. Brief Funct Genomic Proteomic. 3, 382-390 [PUBMED] [PDF] [Abstract...]

First paper on dynamic SILAC in intact animals

Doherty, M.K., Whitehead, C., McCormack, H., Gaskell, S.J. & Beynon, R.J. (2005) Proteome dynamics in complex organisms: using stable isotopes to monitor individual protein turnover rates. Proteomics 5, 522-533 [PUBMED] [PDF] [Abstract...]

Identification of an atypical male-specific MUP in mouse urine

Armstrong, S.D., Robertson, D.H.L., Cheetham, S.A., Hurst, J.L. & Beynon, R.J. (2005) Structural and functional differences in isoforms of Major Urinary Proteins: a male specific protein that preferentially binds a male pheromone. Biochem. J. 391, 343-350 [PUBMED] [PDF] [Abstract...]

Copper toxicity in sheep

Haywood, S. Simpson, D.M, Ross, G. & Beynon, R.J. (2005) The unique response of North Ronaldsay sheep to copper challenge compared with Cambridge sheep: oxidative stress, mitochondrial damage and hepatic stellate cell activation. J. Comp. Path.133, 114-127 [PUBMED] [PDF] [Abstract...]

Scent marks signal competitive ability

Malone, N., Armstrong, S.D., Humphries, R.E., Beynon, R.J. & Hurst, J.L. (2005) The signalling of competitive ability by male house mice. In: Chemical Signals in Vertebrates 10 (Ed. R.T. Mason, M.P. LeMaster & D. Muller-Schwarze), pp.77-88. Springer, New York. [Abstract...]

Characterising proteins in scent marks

Robertson, D.H.L., Cheetham, S.A., Armstrong, S.D., Hurst, J.L. & Beynon, R.J. (2005) Characterisation of proteins in scent marks: proteomics meets semiochemistry. In Chemical Signals in Vertebrates 10 (Ed. R.T. Mason, M.P. LeMaster & D. Muller-Schwarze), pp.183-198 Springer, New York. [Abstract...]

MHC in scent communication

Thom, M.D., Beynon, R.J. & Hurst, J.L. (2005) The role of the major histocompatibility complex in scent communication. In: Chemical Signals in Vertebrates 10 (Ed. R.T. Mason, M.P. LeMaster & D. Muller-Schwarze), pp.173-182. Springer, New York. [Abstract...]

MHC is not involved in individual recognition

Hurst J. L., Thom M. D., Nevison C. M., Humphries R. E., Beynon R. J (2005) MHC odours are not required or sufficient for recognition of individual scent owners. Proc Biol Sci. 272, 715-724 [PUBMED] [PDF] [Abstract...]

Peptide map drawing tool

Beynon, R.J. (2005) A simple tool for drawing proteolytic peptide maps. Bioinformatics 21, 674-675 [PUBMED] [PDF] [Abstract...]

Proteasome proteolysis

Hayter JR, Doherty MK, Whitehead C, McCormack H, Gaskell SJ & Beynon RJ. (2005) The subunit structure and dynamics of the 20S proteasome in chicken skeletal muscle. Mol. Cell. Proteomics. 4, 1370-1381 [PUBMED] [PDF] [Abstract...]

First paper describing QconCAT

Beynon, R.J., Doherty, M.K., Pratt, J.M. & Gaskell, S.J. (2005) Multiplexed absolute quantification in proteomics using artificial QCAT proteins of concatenated signature peptides rates. Nature Methods 2, 587-589 [PUBMED] [PDF [Abstract...]

Muscle proteomics: anabolic agent effects

Burniston JG, McLean L, Beynon RJ, Goldspink DF.   (2007) Anabolic effects of a non-myotoxic dose of the beta(2)-adrenergic receptor agonist clenbuterol on rat plantaris muscle. Muscle & Nerve. 35, 217-223 [PUBMED] [PDF] [Abstract...]

Scent exposure influences competitive behaviour in lab mice

Lacey, J.C. Beynon, R.J. & Hurst, J.L. (2007) The importance of exposure to other male scents in determining competitive behaviour among inbred male mice. Applied Animal Behaviour Science 104, 130-142 [PDF] [Abstract...]

Proteomics of oxidative stress in mice

Aphrodite Vasilaki, Deborah Simpson, Francis McArdle, Lynne McLean, Robert J. Beynon, Holly Van Remmen, Arlan G. Richardson, Anne McArdle, John A Faulkner and Malcolm J. Jackson (2007) Formation of 3-nitrotyrosines in carbonic anhydrase III is a sensitive marker of oxidative stress in skeletal muscle PROTEOMICS – Clinical Applications 1: 362–372 [PDF] [Abstract...]

Proteomics and animal disease

Mary K. Doherty, Robert J. Beynon and Phillip D. Whitfield (2007) Proteomics and naturally occurring animal diseases: Opportunities for animal and human medicine PROTEOMICS – Clinical Applications 2: 135–141 [PDF] [Abstract...]

McArdle disease Cochrane systematic review

Quinlivan R, Beynon RJ, Martinuzzi A. (2008) Pharmacological and nutritional treatment for McArdle disease (Glycogen Storage Disease type V). Cochrane Database Syst Rev. Apr 16;(2):CD003458. [Abstract...]

Proteomics of copper toxicity

Simpson, D. M., Mobasheri, A., Haywood, S. & Beynon, R. J. (2007) A proteomics study of the response of North Ronaldsay sheep to copper challenge. BMC Veterinary Research BMC Vet Res. 27, 36 [PUBMED][PDF] [Abstract...]

Metabolomics in canine hepatology

Whitfield, P.D., Noble, P-J, Major, H., Beynon, R.J., Burrow, R., Freeman, A.L.& German, A. (2006) Metabolomics as a diagnostic tool for hepatology: validation in a naturally occurring canine model. Metabolomics DOI: 10.1007/s11306-005-0001-3 [PDF] [Abstract...]

Mass spectrometry identification of proteins

Nominated in Faculty of 1000 by Harald Stenmark. McCullough, J., Row, P.E. Lorenzo, O, Doherty, M., Beynon, R.J., Clague, M.J. and Urbé, S (2006) Activation of the Endosome-Associated Ubiquitin Isopeptidase AMSH by STAM, a Component of the Multivesicular Body-Sorting Machinery Current Biology 16, 160-165.[PUBMED][PDF] [Abstract...]

Proteome dynamics

Doherty MK, Beynon RJ. (2006) Protein turnover on the scale of the proteome. Expert Rev Proteomics. 1, 97-110[PUBMED] [PDF] [Abstract...]

Proteome turnover: why is it not being studied?

Beynon, R.J. & Pratt, J.M. (2006) Strategies for measuring dynamics: the temporal component of proteomics. Methods Biochem Anal. 2006;49:15-25 [PUBMED] [Abstract...]

QconCAT protocols defined

Pratt, J.M., Simpson, D.M., Doherty, M.K., Rivers, J, Gaskell, S.J. Beynon , R.J. (2006) Multiplexed absolute quantification for proteomics using concatenated signature peptides encoded by QconCAT genes. Nature Protocols 1, 1029 - 1043 [PUBMED][PDF] [Abstract...]

Isolation of N-terminal peptides

Nominated in Faculty of 1000 by Ruedi Aebersold. McDonald, L. & Beynon, R.J. (2006) Positional proteomics: preparation of amino-terminal peptides as a strategy for proteome simplification and characterization. Nature Protocols 1, 1790 - 1798 [PUBMED] [PDF] [Abstract...]

Darcin NMR structure lodged

Phelan MM, McLean L, Simpson DM, Hurst JL, Beynon RJ, Lian LY. (2010) (1)H, (15)N and (13)C resonance assignment of darcin, a mouse major urinary protein. Biomol NMR Assign. 2010 Aug 12[PUBMED] [Abstract...]

The avoidance of inbreeding

Nominated in Faculty of 1000 by Etienne Joly. Sherborne AL, Thom MD, Paterson S, Jury F, Ollier WE, Stockley P, Beynon RJ, Hurst JL. (2007) The genetic basis of inbreeding avoidance in house mice. Curr. Biol. 17, 2061-2066.[PUBMED][PDF] [Abstract...]

McArdle disease treatments

Quinlivan RM, Beynon RJ. (2007) Pharmacological and nutritional treatment trials in McArdle disease. Acta Myol. 26, 58-60. [PUBMED] [Abstract...]

Cauxin in big cat urine

McLean, L., Hurst, J.L., Gaskell, C.J., Lewis, J. C. M & Beynon, R.J. (2007) Characterization of Cauxin in the Urine of Domestic and Big Cats (2007) J. Chem. Ecol 3, 1997-2009 [PUBMED][PDF] [Abstract...]

MUPs in individual recognition

Nominated in Faculty of 1000 by Etienne Joly. Cheetham SA, Thom MD, Jury F, Ollier WE, Beynon RJ, Hurst JL. (2007) The genetic basis of individual-recognition signals in the mouse. Curr. Biol. 17, 1771-1777.[PUBMED][PDF] [Abstract...]

Muscle proteomics

Burniston J G, McLean L, Beynon R J and Goldspink D F (2007) Anabolic effects of a non-myotoxic dose of the beta2-adrenergic receptor agonist clenbuterol on rat plantaris muscle. Muscle Nerve 35, 217-23 [PUBMED][PDF] [Abstract...]

Global cooling

Mclean, L., Young, I.S. Doherty, M.K., Robertson, D.H.L., Cossins, A.R., Gracey, A. Y., Beynon, R.J. & Whitfield, P.D. (2007) Global cooling: Cold acclimation and the expression of soluble proteins in carp skeletal muscle. Proteomics Proteomics 7, 2667-2681 [PUBMED][PDF] [Abstract...]

Pre-eclampsia biomarkers

Myers JE, Hart S, Armstrong S, Mires GJ, Beynon R, Gaskell SJ, Baker PN. (2007) Evidence for multiple circulating factors in preeclampsia. Am J Obstet Gynecol. 196, 266.e1-6. [PUBMED][PDF] [Abstract...]

Significance of animal scents in behaviour

Lacey JC, Beynon RJ, Hurst JL (2007) The importance of exposure to other male scents in determining competitive behaviour among inbred male mice. App Animal Behav Sci 104, 130-142. [PUBMED][PDF] [Abstract...]

Absolute quantification by QconCAT

Rivers J, Simpson DM, Robertson DH, Gaskell SJ, Beynon RJ.(2007) Absolute multiplexed quantitative analysis of protein expression during muscle development using QconCAT. Mol Cell Proteomics. 6, 1416-1427. [PUBMED][PDF] [Abstract...]

Avian Proteomics

Doherty, M.K., Mclean, L. & Beynon, R.J. (2007) Avian Proteomics: Advances, Challenges and New Technologies. Cytogenetic and Genome Research 117, 358-369.[PUBMED][PDF] [Abstract...]

FPGA acceleration of proteomics data processing

Bogdan I, Coca D, Rivers J, Beynon RJ. (2007) Hardware acceleration of processing of mass spectrometric data for proteomics.Bioinformatics. 23, 724-31.[PUBMED][PDF] [Abstract...]

MUPs in an aboriginal mouse

 Robertson, D.H.L., Hurst, J.L., Searle, J.B., Gunduz, I. & Beynon, R.J. (2007) Characterization and Comparison of Major Urinary Proteins from the House Mouse, Mus musculus domesticus, and the Aboriginal Mouse, Mus macedonicus. J. Chem. Ecol. 33, 613-630.[PUBMED][PDF] [Abstract...]

FTICR resolution of similar mass proteins

Robertson DH, Wong SC, Beynon RJ, Hurst JL, Gaskell SJ. (2008) Observation of heterogeneous gene products by FT-ICR MS. J. Am. Soc. Mass Spectrom. 19, 103-110. [PUBMED][PDF] [Abstract...]

Structure and asparagine deamidation

Rivers J, McDonald L, Edwards IJ, Beynon RJ. (2008) Asparagine deamidation and the role of higher order protein structure. J. Proteome Res. 7, 921-7. [PUBMED][PDF] [Abstract...]

Proteomics and animal disease

Doherty, MK; Beynon, RJ; Whitfield, PD (2008): Proteomics and naturally occurring animal diseases: Opportunities for animal and human medicine. Proteomics: Clinical Applications 2, 135-141 [Abstract...]

MUPs and MHC in mate choice

Thom, MD , Stockley, P, Beynon, RJ & Hurst, JL (2008) Scent, mate choice and genetic heterozygosity Chemical Signals in Vertebrates 11, 291-301 [PDF] [Abstract...]

Urinary lipocalins

Beynon, RJ, Hurst, JL, Turton, MJ , Robertson, DHL , Armstrong, SD, Cheetham, SA, Simpson, D, MacNicoll, A, Humphries, RE  (2008) Urinary lipocalins in rodenta: is there a generic model? Chemical Signals in Vertebrates 11, 37-49 [PDF] [Abstract...]

Familiarity breeds...?

Cheetham, SA , Thom, MD, Beynon, RJ & Hurst, JL (2008) The effect of familiarity on mate choice.  Chemical Signals in Vertebrates 11, 271-280 [PDF] [Abstract...]

MUPs advertise heterozyygosity and fitness

Nominated in Faculty of 1000 by Gabrielle Sorci.
Thom MD, Stockley P, Jury F, Ollier WE, Beynon RJ, Hurst JL. (2008) The direct assessment of genetic heterozygosity through scent in the mouse. Curr Biol. 18, 619-623.[PUBMED][PDF] [Abstract...]

Treatment for McArdle disease

Quinlivan R, Beynon RJ, Martinuzzi A. (2008) Pharmacological and nutritional treatment for McArdle disease (Glycogen Storage Disease type V). Cochrane Database Syst Rev.16, CD003458 [PUBMED] [Abstract...]

Speeding up proteomics with hardware computing solutions

Bogdán IA, Rivers J, Beynon RJ, Coca D. (2008) High-performance hardware implementation of a parallel database search engine for real-time peptide mass fingerprinting. Bioinformatics. 24, 1498-1502. [PUBMED][PDF] [Abstract...]

First paper on a systematic analysis of MUP expression

Mudge JM, Armstrong SD, McLaren K, Beynon RJ, Hurst JL, Nicholson C, Robertson DH, Wilming LG, Harrow JL. (2008) Dynamic instability of the major urinary protein gene family revealed by genomic and phenotypic comparisons between C57 and 129 strain mice. [PUBMED][PDF] [Abstract...]

Designer genes for mass spectrometry

Eyers CE, Simpson DM, Wong SC, Beynon RJ, Gaskell SJ. (2008) QCAL-a Novel Standard for Assessing Instrument Conditions for Proteome Analysis. J Am Soc Mass Spectrom. 19, 1275-1280 [PUBMED][PDF] [Abstract...]

FTICR based quantification

Johnson, H., Wong, S C C, D M Simpson, R J Beynon, and S J Gaskell (2008) . Protein quantification by selective isolation and fragmentation of isotopic pairs using FT-ICR MS. J Am Soc Mass Spectrom 19, 973-977 [PUBMED][PDF] [Abstract...]

Biomarkers of ragwort poisoning in horses

Moore RE, Knottenbelt D, Matthews JB, Beynon RJ, Whitfield PD. (2008) Biomarkers for ragwort poisoning in horses: identification of protein targets.BMC Vet Res. 4:30. [PUBMED][PDF] [Abstract...]

Mechanisms of kin recognition

Hurst JL, Beynon RJ. (2010) J Biol. 2010;9(2):13. Making progress in genetic kin recognition among vertebrates. [PUBMED][PDF] [Abstract...]

Proteomics of HGF/EGF signalling

Hammond DE, Hyde R, Kratchmarova I, Beynon RJ, Blagoev B, Clague MJ. (2010) J Proteome Res. 9(5):2734-2742. Quantitative analysis of HGF and EGF-dependent phosphotyrosine signaling networks.[PUBMED][PDF] [Abstract...]

Acetone modifies peptides! Be careful if you're using acetone precipitation.

Simpson DM, Beynon RJ. (2010) Acetone precipitation of proteins and the modification of peptides. J Proteome Res. 9, 444-450 [PUBMED][PDF] [Abstract...]

Cross-species proteomics

Wright JC, Beynon RJ, Hubbard SJ. Cross species proteomics. (2010) Methods Mol Biol. 604:123-135 [PUBMED][PDF] [Abstract...]

Hardware accelerated proteomics

Coca D, Bogdan I, Beynon RJ. A high-performance reconfigurable computing solution for peptide mass fingerprinting. (2010) Methods Mol Biol. 604:163-185 [PUBMED][PDF] [Abstract...]

Phosphorylation stoichiometry

Rigorous determination of the stoichiometry of protein phosphorylation using mass spectrometry (2009) Johnson H, Eyers CE, Eyers PA, Beynon RJ, Gaskell SJ. J Am Soc Mass Spectrom. 20, 2211-2220 [PUBMED][PDF] [Abstract...]

Proteomics and genome annotation

Wright, J.C., Sugden, D., Francis-McIntyre, S., Riba-Gacia, I., Gaskell, S.J., Grigoriev, I V., Baker, S.E., Beynon, R.J. & Hubbard, S.J. (2009)Exploiting proteomic data for genome annotation and gene model validation in Aspergillus niger. BMC Genomics. 10, 61 [PUBMED][PDF] [Abstract...]

MUP variation in lab mouse strains

Cheetham SA, Smith AL, Armstrong SD, Beynon RJ, Hurst JL. (2009) Limited variation in the major urinary proteins of laboratory mice. Physiol Behav. 96, 253-261 [PUBMED][PDF] [Abstract...]

Parasite proteomics

Smith, S.K., Nisbet, A.J., Meikle, L.I., Inglis, N.F., Sales, J., Beynon, R.J. & Matthews, J.B. (2010) Proteomic analysis of excretory/secretory products released by Teladorsagia circumcincta larvae early post-infection. Parasite Immunology 31, 10-19 [PUBMED][PDF] [Abstract...]

Dynamic SILAC for global assessment of proteome turnover

Doherty, M.K., Hammond, D., Clague, M. J., Gaskell, S.J. & Beynon, R.J. (2009) Turnover of the human proteome: determination of protein intracellular stability by dynamic SILAC. J. Proteome Res. 8, 104-112 [PUBMED] [PDF] [Abstract...]

Seminal vesicle protein evolution

Ramm, S.A., McDonald, L.M., Hurst, JL., Beynon, R.J. & Stockley, P.S. (2009) Comparative Proteomics Reveals Evidence for Evolutionary Diversification of Rodent Seminal Fluid and Its Functional Significance in Sperm Competition. Mol. Biol. Evol. 26, 189-198 [PUBMED] [PDF] [Abstract...]

Even the smallest of hamsters have lipocalins in their urine.

Turton MJ, Robertson DH, Smith JR, Hurst JL, Beynon RJ. Roborovskin, a Lipocalin in the Urine of the Roborovski Hamster, Phodopus roborovskii. Chem Senses. 2010 Jun 24. [PUBMED][PDF] [Abstract...]

It is a truth, universally acknowledged, that a mouse in possession of darcin must be in want of a mate.

Nominated as 'Exceptional' by Etienne Joly in Faculty1000 Biology (http://f1000biology.com)
Roberts SA, Simpson, Armstrong, Davidson, Robertson, McLean, Beynon & Hurst JL (2010) Darcin: a male pheromone that stimulates female memory and sexual attraction to an individual male's odour. BMC Biology 8:75. [PUBMED][PDF] [Abstract...]