PhosphoLFQ of NF-kB/RelA in DNA damage

Amy E Campbell, Caterina Ferraz Franco, Ling-I Su, Emma K Corbin, Simon Perkins, Anton Kalyuzhnyy, Andrew R Jones, Philip J Brownridge, Neil D Perkins, Claire E Eyers (2021) Temporal modulation of the NF-κB RelA network in response to different types of DNA damage Biochem J doi: 10.1042/BCJ20200627. Online ahead of print. [Abstract...]

SILAC Phosphoproteomics

Dominic P Byrne, Christopher J Clarke, Philip J Brownridge, Anton Kalyuzhnyy, Simon Perkins, Amy Campbell, David Mason, Andrew R Jones, Patrick A Eyers, Claire E Eyers (2020) Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signalling networks using SILAC-based phosphoproteomics [PUBMED] [PDF] [DOI] [Abstract...]

Regulation of Aurora A by CoASH

Yugo Tsuchiya, Dominic P Byrne, Selena G Burgess, Jenny Bormann, Jovana Baković, Yueyang Huang, Alexander Zhyvoloup, Bess Yi Kun Yu, Sew Peak-Chew, Trang Tran, Fiona Bellany, Alethea B Tabor, Aw Edith Chan, Lalitha Guruprasad, Oleg Garifulin, Valeriy Filonenko, Matthias Vonderach, Samantha Ferries, Claire E Eyers, John Carroll, Mark Skehel, Richard Bayliss , Patrick A Eyers, Ivan Gout (2020) Covalent Aurora A regulation by the metabolic integrator coenzyme A. [PDF] [PUBMED] [DOI] [Abstract...]

Methodology for hunting histidine phosphorylation

Gemma Hardman and Claire E. Eyers (2020) High-Throughput Characterization of Histidine Phosphorylation Sites Using UPAX and Tandem Mass Spectrometry. [PDF] [PUBMED] [DOI] [Abstract...]

Redox control of protein kinases

Dominic P Byrne, Safal Shrestha , Martin Galler, Min Cao, Leonard A Daly, Amy E Campbell, Claire E Eyers, Elizabeth A Veal, Natarajan Kannan Patrick A Eyers (2020) Aurora A regulation by reversible cysteine oxidation reveals evolutionarily conserved redox control of Ser/Thr protein kinase activity. [PDF] [PUBMED] [DOI] [Abstract...]

Discovery and definition of non-canonical phosphorylation

Gemma Hardman , Simon Perkins , Philip Brownridge, Christopher J. Clarke , Dominic P. Byrne, Amy E. Campbell , Anton Kalyuzhnyy , Ashleigh Myall , Patrick A. Eyers , Andrew R. Jones and Claire E. Eyers (2019) Strong anion exchange-mediated phosphoproteomics reveals extensive human non-canonical phosphorylation [PUBMED] [PDF] [DOI]

Myoblast phosphoproteomics

Fiona K. Jones, Gemma E. Hardman, Samantha Ferries, Claire E. Eyers, and Addolorata Pisconti (2019) Myoblast Phosphoproteomics as a Tool to Investigate Global Signaling Events During Myogenesis . [PUBMED] [PDF] [Abstract...]

Deep discovery of novel phosphorylation sites

Gemma Hardman, Simon Perkins, Zheng Ruan, Natarajan Kannan, Philip Brownridge, Dominic P Byrne, Patrick A Eyers, Andrew R Jones (2017) Extensive non-canonical phosphorylation in human cells revealed using strong-anion exchange-mediated phosphoproteomics [PDF] [BIORXIV] [Abstract...]

Enhanced ability in phosphosite localisation

Ferries S, Perkins S, Brownridge PJ, Campbell A, Eyers PA, Jones AR, Eyers CE. (2017) Evaluation of Parameters for Confident Phosphorylation Site Localization Using an Orbitrap Fusion Tribrid Mass Spectrometer. [PDF] [PUBMED] [Abstract...]

Kinases in spindle assembly

Bury L, Coelho PA, Simeone A, Ferries S, Eyers CE, Eyers PA, Zernicka-Goetz M, Glover DM. (2017) Plk4 and Aurora A cooperate in the initiation of acentriolar spindle assembly in mammalian oocytes. [PDF] [PUBMED] [Abstract...]

New insights into protein kinase A action

Smith FD, Esseltine JL, Nygren PJ, Veesler D, Byrne DP, Vonderach M, Strashnov I, Eyers CE, Eyers PA, Langeberg LK, Scott JD. (2017) Local protein kinase A action proceeds through intact holoenzymes. [PUBMED] [PDF] [Abstract...]

Tools for kinome research

McSkimming DI, Dastgheib S, Baffi TR, Byrne DP, Ferries S, Scott ST, Newton AC, Eyers CE, Kochut KJ, Eyers PA, Kannan N. (2016) KinView: a visual comparative sequence analysis tool for integrated kinome research. [PUBMED] [PDF] [Abstract...]

Complexity of RelA phosphorylation

Lanucara F, Lam C, Mann J, Monie TP, Colombo SA, Holman SW, Boyd J, Dange MC, Mann DA, White MR, Eyers CE. (2016) Dynamic phosphorylation of RelA on Ser42 and Ser45 in response to TNFα stimulation regulates DNA binding and transcription. [PDF] [PUBMED] [Abstract...]

Unblocking the sink

Lanucara F, Chi Hoo Lee D, Eyers CE. (2013) Unblocking the Sink: Improved CID-Based Analysis of Phosphorylated Peptides by Enzymatic Removal of the Basic C-Terminal Residue. [PDF][PUBMED] [Abstract...]

MS for identification of PTMs

Eyers, Claire E., Gaskell, Simon J. 2008. Mass spectrometry to identify posttranslational modifications. Wiley Encyclopedia of Chemical Biology. [Abstract...]

CaZIP phosphorylation

Eyers, Claire E., McNeill, Helen, Knebel, Axel, Morrice, Nick, Arthur, Simon J. C., Cuenda, Ana, Cohen, Philip. 2005. The phosphorylation of CapZ-interacting protein (CapZIP) by stress-activated protein kinases triggers its dissociation from CapZ. Biochemical Journal 389: 127. [PUBMED] [Abstract...]

iMAC for phosphorylation sites

Haydon, Claire E., Eyers, Patrick A., Aveline-Wolf, Lauren D., Resing, Katheryn A., Maller, James L., Ahn, Natalie G. 2003. Identification of novel phosphorylation sites on Xenopus laevis Aurora A and analysis of phosphopeptide enrichment by immobilized metal-affinity chromatography. Molecular & Cellular Proteomics 2: 1055-1067. [PUBMED] [Abstract...]

EF2 kinase regulation

Knebel, Axel, Haydon, Claire E., Morrice, Nick, Cohen, Philip. 2002. Stress-induced regulation of eukaryotic elongation factor 2 kinase by SB 203580-sensitive and-insensitive pathways. Biochemical Journal 367: 525. [PUBMED] [Abstract...]

Myosin light chain kinase phosphorylation site

Haydon, Claire E., Watt, Peter W., Morrice, Nick, Knebel, Axel, Gaestel, Matthias, Cohen, Philip. 2002. Identification of a phosphorylation site on skeletal muscle myosin light chain kinase that becomes phosphorylated during muscle contraction. Archives of Biochemistry and Biophysics 397: 224-231. [PUBMED] [Abstract...]