2020

First 'zap the crap', now 'Fry the fly': REIMS for insect identification

Iris Wagner, Natalie I. Koch, Joscelyn Sarsby, Nicola White, Tom A. R. Price, Sam Jones, Jane L. Hurst, Robert J. Beynon, (2020) The application of rapid evaporative ionisation mass spectrometry (REIMS) in the analysis of Drosophila species – a potential new tool in entomology [PDF] [PUBMED] [DOI] [Abstract...]

Norovirus mode of action

Myra Hosmillo , Jia Lu, Michael R McAllaster, James B Eaglesham, Xinjie Wang, Edward Emmott, Patricia Domingues, Yasmin Chaudhry, Tim J Fitzmaurice, Matthew Kh Tung, Marc Dominik Panas, Gerald McInerney, Nicolas Locker, Craig B Wilen, Ian G Goodfellow (2020) Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation [PDF] [PUBMED] [DOI] [Abstract...]

Using stable isotopes to track sperm competition

Paula Stockley, Catarina Franco, Amy J. Claydon, Amanda Davidson, Dean E. Hammond, Philip J. Brownridge, Jane L. Hurst, and Robert J. Beynon (2020) Revealing mechanisms of mating plug function under sexual selection. [PDF] [PUBMED] [DOI] [Abstract...]

Plasticity of the mouse seminal vesical fluid proteome

Helen L Bayram, Catarina Franco, Philip Brownridge, Amy J Claydon, Natalie Koch, Jane L Hurst, Robert J Beynon, Paula Stockley (2020) Social status and ejaculate composition in the house mouse [PDF] [PUBMED] [DOI] [Abstract...]

SILAC Phosphoproteomics

Dominic P Byrne, Christopher J Clarke, Philip J Brownridge, Anton Kalyuzhnyy, Simon Perkins, Amy Campbell, David Mason, Andrew R Jones, Patrick A Eyers, Claire E Eyers (2020) Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signalling networks using SILAC-based phosphoproteomics [PUBMED] [PDF] [DOI] [Abstract...]

Novel proteolytic events in SARS-Cov-2 infection

Bjoern Meyer, Jeanne Chiaravalli, Philip Brownridge, Dominic P. Byrne, Leonard A. Daly, Fabrice Agou, Claire E. Eyers, Patrick A. Eyers, Marco Vignuzzi, Edward Emmott (2020) Characterisation of protease activity during SARS-CoV-2 infection identifies novel viral cleavage sites and cellular targets for drug repurposing [PREPRINT] [Abstract...]

The COVID-19 MS coalition

Weston Struwe, Edward Emmott, Melanie Bailey, Michal Sharon, Andrea Sinz, Fernando J Corrales, Kostas Thalassinos, Julian Braybrook, Clare Mills, Perdita Barran, COVID-19 MS Coalition (2020) The COVID-19 MS Coalition-accelerating diagnostics, prognostics, and treatment. [PDF] [PUBMED] [DOI] [Abstract...]

Temporal proteomics of herpes virus infection

Timothy K Soh, Colin T R Davies, Julia Muenzner, Leah M Hunter, Henry G Barrow, Viv Connor, Clément R Bouton, Cameron Smith, Edward Emmott, Robin Antrobus, Stephen C Graham, Michael P Weekes, Colin M Crump(2020) Temporal Proteomic Analysis of Herpes Simplex Virus 1 Infection Reveals Cell-Surface Remodeling via pUL56-Mediated GOPC Degradation. [PDF] [PUBMED] [DOI] [Abstract...]

Using QconCATs to establish protein complex stoichiometry

Mengru Yang, Deborah M Simpson, Nicolas Wenner, Philip Brownridge, Victoria M Harman, Jay C D Hinton, Robert J Beynon, Lu-Ning Liu (2020) Decoding the stoichiometric composition and organisation of bacterial metabolosomes [PDF] [PUBMED] [DOI] [Abstract...]

Regulation of Aurora A by CoASH

Yugo Tsuchiya, Dominic P Byrne, Selena G Burgess, Jenny Bormann, Jovana Baković, Yueyang Huang, Alexander Zhyvoloup, Bess Yi Kun Yu, Sew Peak-Chew, Trang Tran, Fiona Bellany, Alethea B Tabor, Aw Edith Chan, Lalitha Guruprasad, Oleg Garifulin, Valeriy Filonenko, Matthias Vonderach, Samantha Ferries, Claire E Eyers, John Carroll, Mark Skehel, Richard Bayliss , Patrick A Eyers, Ivan Gout (2020) Covalent Aurora A regulation by the metabolic integrator coenzyme A. [PDF] [PUBMED] [DOI] [Abstract...]

Stoichiometry of histidine kinases by mass spectrometry

Lauren J Tomlinson, Alice K M Clubbs Coldron , Patrick A Eyers, Claire E Eyers (2020) Determination of Phosphohistidine Stoichiometry in Histidine Kinases by Intact Mass Spectrometry. [PDF] [PUBMED] [DOI] [Abstract...]

Ion mobility to evaluate ligand binding to proteins

Lauren J Tomlinson, Claire E Eyers (2020) Ion Mobility-Mass Spectrometry to Evaluate the Effects of Protein Modification or Small Molecule Binding on Protein Dynamics. [PDF] [PUBMED] [DOI] [Abstract...]

MS from gels, top down or bottom up

Ayako Takemori, David S Butcher, Victoria M Harman, Philip Brownridge, Keisuke Shima, Daisuke Higo, Jun Ishizaki, Hitoshi Hasegawa, Junpei Suzuki, Masakatsu Yamashita, Joseph A Loo, Rachel R Ogorzalek Loo, Robert J Beynon, Lissa C Anderson, Nobuaki Takemori (2020) PEPPI-MS: Polyacrylamide-Gel-Based Prefractionation for Analysis of Intact Proteoforms and Protein Complexes by Mass Spectrometry [PDF] [PUBMED] [DOI] [Abstract...]

An unusual marsupial urinary lipocalin - vulpeculin

Grace M. Loxley, David O. Hooks, Aristotelis Antonopoulos, Anne Dell, Stuart M. Haslam, Wayne L. Linklater, Jane L. Hurst and Robert J. Beynon (2020) Vulpeculin: a novel and abundant lipocalin in the urine of the common brushtail possum, Trichosurus vulpecula. Open Biology Open Biol. 10: 200218. http://dx.doi.org/10.1098/rsob.200218 [PDF] [PUBMED] [DOI] [Abstract...]

Methodology for hunting histidine phosphorylation

Gemma Hardman and Claire E. Eyers (2020) High-Throughput Characterization of Histidine Phosphorylation Sites Using UPAX and Tandem Mass Spectrometry. [PDF] [PUBMED] [DOI] [Abstract...]

Using proteomics to explore 'pseudogene' expression

Ian Goodhead, Frances Blow, Philip Brownridge, Margaret Hughes, John Kenny, Ritesh Krishna, Lynn McLean, Pisut Pongchaikul, Rob Beynon and Alistair C. Darby (202o) Large-scale and significant expression from pseudogenes in Sodalis glossinidius – a facultative bacterial endosymbiont [PDF] [PUBMED] [DOI] [Abstract...]

Global proteomics of carbon source metabolism in Saccharomyces

M Garcia-Albornoz 1, S W Holman, T Antonisse, P Daran-Lapujade, B Teusink, R J Beynon, S J Hubbard (2020) A proteome-integrated, carbon source dependent genetic regulatory network in Saccharomyces cerevisiae. [PDF] [PUBMED] [DOI] [Abstract...]

Connective tissue protein turnover


Howard Choi, Deborah Simpson, Ding Wang, Mark Prescott, Andrew A Pitsillides, Jayesh Dudhia, Peter D Clegg, Peipei Ping, Chavaunne T Thorpe (2020) Heterogeneity of proteome dynamics between connective tissue phases of adult tendon. [
PDF] [PUBMED] [DOI] [Abstract...]

Darcin and the neural circuits it activates

Ebru Demir, Kenneth Li, Natasha Bobrowski-Khoury, Joshua I Sanders, Robert J Beynon, Jane L Hurst, Adam Kepecs, Richard Axel (2020) The pheromone darcin drives a circuit for innate and reinforced behaviours [PDF] [PUBMED] [DOI] [Abstract...]

Using proteomics to identify targets for schistosomiasis

Leandro X Neves, R Alan Wilson, Philip Brownridge, Victoria M Harman, Stephen W Holman, Robert J Beynon, Claire E Eyers, Ricardo DeMarco, William Castro-Borges (2020) Quantitative Proteomics of Enriched Esophageal and Gut Tissues from the Human Blood Fluke Schistosoma mansoni Pinpoints Secreted Proteins for Vaccine Development. [PDF] [PUBMED] [DOI] [Abstract...]

Redox control of protein kinases

Dominic P Byrne, Safal Shrestha , Martin Galler, Min Cao, Leonard A Daly, Amy E Campbell, Claire E Eyers, Elizabeth A Veal, Natarajan Kannan Patrick A Eyers (2020) Aurora A regulation by reversible cysteine oxidation reveals evolutionarily conserved redox control of Ser/Thr protein kinase activity. [PDF] [PUBMED] [DOI] [Abstract...]