Photo of Dr Igor Barsukov

Dr Igor Barsukov PhD

Reader in Biomolecular NMR Biochemistry & Systems Biology

    Publications

    2019

    A further unique chondroitin sulfate from the shrimp Litopenaeus vannamei with antithrombin activity that modulates acute inflammation (Journal article)

    Palhares, L. C. G. F., Brito, A. S., de Lima, M. A., Nader, H. B., London, J. A., Barsukov, I. L., . . . Chavante, S. F. (2019). A further unique chondroitin sulfate from the shrimp Litopenaeus vannamei with antithrombin activity that modulates acute inflammation. CARBOHYDRATE POLYMERS, 222. doi:10.1016/j.carbpol.2019.115031

    DOI: 10.1016/j.carbpol.2019.115031

    2018

    Co-ordination of mechanotransduction by talin and vinculin (Conference Paper)

    Atherton, P., Lausecker, F., Barsukov, I., Critchley, D., & Ballestrem, C. (2018). Co-ordination of mechanotransduction by talin and vinculin. In INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY Vol. 99 (pp. A21). Retrieved from http://gateway.webofknowledge.com/

    New tools for carbohydrate sulfation analysis: heparan sulfate 2-O-sulfotransferase (HS2ST) is a target for small-molecule protein kinase inhibitors (Journal article)

    Byrne, D. P., Li, Y., Ramakrishnan, K., Barsukov, I. L., Yates, E. A., Eyers, C. E., . . . Eyers, P. A. (2018). New tools for carbohydrate sulfation analysis: heparan sulfate 2-O-sulfotransferase (HS2ST) is a target for small-molecule protein kinase inhibitors. BIOCHEMICAL JOURNAL, 475(15), 2417-2433. doi:10.1042/BCJ20180265

    DOI: 10.1042/BCJ20180265

    New tools for carbohydrate sulphation analysis: Heparan Sulphate 2-O-sulphotranserase (HS2ST) is a target for small molecule protein kinase inhibitors (Journal article)

    Byrne, D. P., Li, Y., Ramakrishnan, K., Barsukov, I. L., Yates, E. A., Eyers, C. E., . . . Eyers, P. A. (n.d.). New tools for carbohydrate sulphation analysis: Heparan Sulphate 2-O-sulphotranserase (HS2ST) is a target for small molecule protein kinase inhibitors. doi:10.1101/296533

    DOI: 10.1101/296533

    2017

    Pressure-Dependent Chemical Shifts in the R3 Domain of Talin Show that It Is Thermodynamically Poised for Binding to Either Vinculin or RIAM (Journal article)

    Baxter, N. J., Zacharchenko, T., Barsukov, I. L., & Williamson, M. P. (2017). Pressure-Dependent Chemical Shifts in the R3 Domain of Talin Show that It Is Thermodynamically Poised for Binding to Either Vinculin or RIAM. Structure, 25(12), 1856-1866.e2. doi:10.1016/j.str.2017.10.008

    DOI: 10.1016/j.str.2017.10.008

    Targeting SxIP-EB1 interaction: An integrated approach to the discovery of small molecule modulators of dynamic binding sites (Journal article)

    Almeida, T. B., Carnell, A. J., Barsukov, I. L., & Berry, N. G. (2017). Targeting SxIP-EB1 interaction: An integrated approach to the discovery of small molecule modulators of dynamic binding sites. SCIENTIFIC REPORTS, 7. doi:10.1038/s41598-017-15502-6

    DOI: 10.1038/s41598-017-15502-6

    SHANK proteins limit integrin activation by directly interacting with Rap1 and R-Ras (Journal article)

    Lilja, J., Zacharchenko, T., Georgiadou, M., Jacquemet, G., Franceschi, N. D., Peuhu, E., . . . Ivaska, J. (2017). SHANK proteins limit integrin activation by directly interacting with Rap1 and R-Ras. Nature Cell Biology, 19(4), 292-305. doi:10.1038/ncb3487

    DOI: 10.1038/ncb3487

    2016

    Biophysical Analysis of the N-Terminal Domain from the Human Protein Phosphatase 1 Nuclear Targeting Subunit PNUTS Suggests an Extended Transcription Factor TFIIS-Like Fold (Journal article)

    Zacharchenko, T., Barsukov, I., Rigden, D. J., Bennett, D., & Mayans, O. (2016). Biophysical Analysis of the N-Terminal Domain from the Human Protein Phosphatase 1 Nuclear Targeting Subunit PNUTS Suggests an Extended Transcription Factor TFIIS-Like Fold. Protein Journal, 35(5), 240-345. doi:10.1007/s10930-016-9677-7

    DOI: 10.1007/s10930-016-9677-7

    CARP interacts with titin at a unique helical N2A sequence and at the domain Ig81 to form a structured complex (Journal article)

    Zhou, T., Fleming, J. R., Franke, B., Bogomolovas, J., Barsukov, I., Rigden, D. J., . . . Mayans, O. (2016). CARP interacts with titin at a unique helical N2A sequence and at the domain Ig81 to form a structured complex. FEBS LETTERS, 590(18), 3098-3110. doi:10.1002/1873-3468.12362

    DOI: 10.1002/1873-3468.12362

    LD Motif Recognition by Talin: Structure of the Talin-DLC1 Complex (Journal article)

    Zacharchenko, T., Qian, X., Goult, B. T., Jethwa, D., Almeida, T. B., Ballestrem, C., . . . Barsukov, I. L. (2016). LD Motif Recognition by Talin: Structure of the Talin-DLC1 Complex. Structure, 24(7), 1130-1141. doi:10.1016/j.str.2016.04.016

    DOI: 10.1016/j.str.2016.04.016

    SHANK3 Structure Reveals a RAS-associated Domain Regulating Integrin Activation. (Conference Paper)

    Lilja, J., Zacharchenko, T., Georgiadou, M., Kreienkamp, H., Barsukov, I., & Ivaska, J. (2016). SHANK3 Structure Reveals a RAS-associated Domain Regulating Integrin Activation.. In MOLECULAR BIOLOGY OF THE CELL Vol. 27. Retrieved from http://gateway.webofknowledge.com/

    SHANK3 Structure Reveals a RAS-associated Domain Regulating Integrin Activation. (Conference Paper)

    Lilja, J., Zacharchenko, T., Georgiadou, M., Kreienkamp, H., Barsukov, I., & Ivaska, J. (2016). SHANK3 Structure Reveals a RAS-associated Domain Regulating Integrin Activation.. In MOLECULAR BIOLOGY OF THE CELL Vol. 27. Retrieved from http://gateway.webofknowledge.com/

    2015

    Vinculin controls talin engagement with the actomyosin machinery. (Journal article)

    Atherton, P., Stutchbury, B., Wang, D. -Y., Jethwa, D., Tsang, R., Meiler-Rodriguez, E., . . . Ballestrem, C. (2015). Vinculin controls talin engagement with the actomyosin machinery.. Nature communications, 6. doi:10.1038/ncomms10038

    DOI: 10.1038/ncomms10038

    Parallel evolution or purifying selection, not introgression, explains similarity in the pyrethroid detoxification linked GSTE4 of Anopheles gambiae and An. arabiensis (Journal article)

    Wilding, C. S., Weetman, D., Rippon, E. J., Steen, K., Mawejje, H. D., Barsukov, I., & Donnelly, M. J. (2015). Parallel evolution or purifying selection, not introgression, explains similarity in the pyrethroid detoxification linked GSTE4 of Anopheles gambiae and An. arabiensis. MOLECULAR GENETICS AND GENOMICS, 290(1), 201-215. doi:10.1007/s00438-014-0910-9

    DOI: 10.1007/s00438-014-0910-9

    2014

    S100A4 regulates the Src-tyrosine kinase dependent differentiation of Th17 cells in rheumatoid arthritis (Journal article)

    Brisslert, M., Bian, L., Svensson, M. N. D., Santos, R. F., Jonsson, I. -M., Barsukov, I., . . . Bokarewa, M. I. (2014). S100A4 regulates the Src-tyrosine kinase dependent differentiation of Th17 cells in rheumatoid arthritis. Biochimica et Biophysica Acta - Molecular Basis of Disease, 1842(11), 2049-2059. doi:10.1016/j.bbadis.2014.07.003

    DOI: 10.1016/j.bbadis.2014.07.003

    S100A4 REGULATES THE SRC-THYROSINE KINASE DEPENDENT DIFFERENTIATION OF TH17 CELLS IN RHEUMATOID ARTHRITIS (Conference Paper)

    Brisslert, M., Bian, L., Svensson, M., Santos, R., Jonsson, I. -M., Erlandsson, M., . . . Bokarewa, M. (2014). S100A4 REGULATES THE SRC-THYROSINE KINASE DEPENDENT DIFFERENTIATION OF TH17 CELLS IN RHEUMATOID ARTHRITIS. In ANNALS OF THE RHEUMATIC DISEASES Vol. 73 (pp. 129). doi:10.1136/annrheumdis-2014-eular.5414

    DOI: 10.1136/annrheumdis-2014-eular.5414

    2013

    Deubiquitylases From Genes to Organism (Journal article)

    Clague, M. J., Barsukov, I., Coulson, J. M., Liu, H., Rigden, D. J., & Urbé, S. (2013). Deubiquitylases From Genes to Organism. Physiological Reviews, 93(3), 1289-1315. doi:10.1152/physrev.00002.2013

    DOI: 10.1152/physrev.00002.2013

    'RIAM and vinculin binding to talin are mutually exclusive and regulate adhesion assembly and turnover' (Journal article)

    Goult, B. T., Zacharchenko, T., Bate, N., Tsang, R., Hey, F., Gingras, A. R., . . . Barsukov, I. L. (2013). 'RIAM and vinculin binding to talin are mutually exclusive and regulate adhesion assembly and turnover'. Journal of Biological Chemistry, 288(12), 8238-8249.

    'SHANK3 Gene Mutations Associated with Autism Facilitate Ligand Binding to the Shank3 Ankyrin Repeat Region' (Journal article)

    Mameza, M. G., Dvoretskova, E., Bamann, M., Hönck, H. H., Güler, T., Boeckers, T. M., . . . Kreienkamp, H. J. (2013). 'SHANK3 Gene Mutations Associated with Autism Facilitate Ligand Binding to the Shank3 Ankyrin Repeat Region'. Journal of Biological Chemistry.

    'Structural studies on full-length talin1 reveal a compact auto-inhibited dimer: Implications for talin activation' (Journal article)

    Goult, B. T., Xu, X. P., Gingras, A. R., Swift, M., Patel, B., Bate, N., . . . Hanein, D. (2013). 'Structural studies on full-length talin1 reveal a compact auto-inhibited dimer: Implications for talin activation'. Journal of Structural Biology.

    2012

    The intracellular Ig fold: a robust protein scaffold for the engineering of molecular recognition (Journal article)

    Bruning, M., Barsukov, I., Franke, B., Barbieri, S., Volk, M., Leopoldseder, S., . . . Mayans, O. (2012). The intracellular Ig fold: a robust protein scaffold for the engineering of molecular recognition. Protein Engineering Design and Selection, 25(5), 205-212. doi:10.1093/protein/gzs007

    DOI: 10.1093/protein/gzs007

    Asymmetric Mode of Ca2+-S100A4 Interaction with Nonmuscle Myosin IIA Generates Nanomolar Affinity Required for Filament Remodeling (Journal article)

    Elliott, P. R., Irvine, A. F., Jung, H. S., Tozawa, K., Pastok, M. W., Picone, R., . . . Barsukov, I. L. (2012). Asymmetric Mode of Ca2+-S100A4 Interaction with Nonmuscle Myosin IIA Generates Nanomolar Affinity Required for Filament Remodeling. STRUCTURE, 20(4), 654-666. doi:10.1016/j.str.2012.02.002

    DOI: 10.1016/j.str.2012.02.002

    2011

    Structural variability of the ubiquitin specific protease DUSP-UBL double domains (Journal article)

    Elliott, P. R., Liu, H., Pastok, M. W., Grossmann, G. J., Rigden, D. J., Clague, M. J., . . . Barsukov, I. L. (2011). Structural variability of the ubiquitin specific protease DUSP-UBL double domains. FEBS LETTERS, 585(21), 3385-3390. doi:10.1016/j.febslet.2011.09.040

    DOI: 10.1016/j.febslet.2011.09.040

    A study of the membrane association and regulatory effect of the phospholemman cytoplasmic domain (Journal article)

    Hughes, E., Whittaker, C. A. P., Barsukov, I. L., Esmann, M., & Middleton, D. A. (2011). A study of the membrane association and regulatory effect of the phospholemman cytoplasmic domain. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1808(4), 1021-1031. doi:10.1016/j.bbamem.2010.11.024

    DOI: 10.1016/j.bbamem.2010.11.024

    Mechanism of the Ca²+-dependent interaction between S100A4 and tail fragments of nonmuscle myosin heavy chain IIA (Journal article)

    Badyal, S. K., Basran, J., Bhanji, N., Kim, J. H., Chavda, A. P., Jung, H. S., . . . Bagshaw, C. R. (2011). Mechanism of the Ca²+-dependent interaction between S100A4 and tail fragments of nonmuscle myosin heavy chain IIA. J Mol Biol, 405, 1004-1026.

    Resonance Assignments (Chapter)

    Lian, L. Y., & Barsukov, I. L. (2011). Resonance Assignments. In L. -Y. Lian, & G. Roberts (Eds.), Protein NMR Spectros (pp. 55-82). Chichester: Willey.

    2010

    The Structure of the Talin Head Reveals a Novel Extended Conformation of the FERM Domain (Journal article)

    Elliott, P. R., Goult, B. T., Kopp, P. M., Bate, N., Grossmann, J. G., Roberts, G. C. K., . . . Barsukov, I. L. (2010). The Structure of the Talin Head Reveals a Novel Extended Conformation of the FERM Domain. Structure, 18(10), 1289-1299. doi:10.1016/j.str.2010.07.011

    DOI: 10.1016/j.str.2010.07.011

    Structure of a double ubiquitin-like domain in the talin head: a role in integrin activation (Journal article)

    Goult, B. T., Bouaouina, M., Elliott, P. R., Bate, N., Patel, B., Gingras, A. R., . . . Barsukov, I. L. (2010). Structure of a double ubiquitin-like domain in the talin head: a role in integrin activation. The EMBO Journal, 29(6), 1069-1080. doi:10.1038/emboj.2010.4

    DOI: 10.1038/emboj.2010.4

    Central Region of Talin Has a Unique Fold That Binds Vinculin and Actin (Journal article)

    Gingras, A. R., Bate, N., Goult, B. T., Patel, B., Kopp, P. M., Emsley, J., . . . Critchley, D. R. (2010). Central Region of Talin Has a Unique Fold That Binds Vinculin and Actin. J Biol Chem, 285, 29577-29587.

    Structure of a double ubiquitin-like domain in the talin head: a role in integrin activation (Journal article)

    Goult, B. J., Bouaouina, M., Elliott, P. R., Bate, N., Patel, B., Gingras, A. R., . . . Barsukov, I. L. (2010). Structure of a double ubiquitin-like domain in the talin head: a role in integrin activation. EMBO Journal, 29(6), 1069-1080.

    2009

    The Structure of the N-Terminus of Kindlin-1: A Domain Important for aIIbß3 Integrin Activation (Journal article)

    Goult, B. T., Bouaouina, M., Harburger, D. S., Bate, N., Patel, B., Anthis, N. J., . . . Critchley, D. R. (2009). The Structure of the N-Terminus of Kindlin-1: A Domain Important for aIIbß3 Integrin Activation. Journal of Molecular Biology, 394(5), 944-956. doi:10.1016/j.jmb.2009.09.061

    DOI: 10.1016/j.jmb.2009.09.061

    The Structure of an Interdomain Complex That Regulates Talin Activity (Journal article)

    Goult, B. T., Bate, N., Anthis, N. J., Wegener, K. L., Gingras, A. R., Patel, B., . . . Critchley, D. R. (2009). The Structure of an Interdomain Complex That Regulates Talin Activity. Journal of Biological Chemistry, 284(22), 15097-15106. doi:10.1074/jbc.m900078200

    DOI: 10.1074/jbc.m900078200

    'Domain motion in cytochrome P450 reductase' (Journal article)

    Ellis, J., Gutierrez, A., Barsukov, I. L., Huang, W. C., Grossmann, J. G., & Roberts, G. C. K. (2009). 'Domain motion in cytochrome P450 reductase'. The Journal of Biological Chemistry, 284(52), 36628-36637.

    'Structural determinants of integrin binding to the talin rod' (Journal article)

    Gingras, A. R., Ziegler, W. H., Bobkov, A. A., Joyce, M. G., Fasci, D., Himmel, M., . . . Critchley, D. R. (2009). 'Structural determinants of integrin binding to the talin rod'. The journal of Biological Chemistry, 284(13), 8866-8876.

    Structural determinants of integrin binding to the talin rod. (Journal article)

    Gingras, A. R., Ziegler, W. H., Bobkov, A. A., Joyce, M. G., Fasci, D., Himmel, M., . . . Critchley, D. R. (2009). Structural determinants of integrin binding to the talin rod.. J Biol Chem, 284(13), 8866-8876.

    2008

    Structural Basis for the Interaction between the Cytoplasmic Domain of the Hyaluronate Receptor Layilin and the Talin F3 Subdomain (Journal article)

    Wegener, K. L., Basran, J., Bagshaw, C. R., Campbell, I. D., Roberts, G. C. K., Critchley, D. R., & Barsukov, I. L. (2008). Structural Basis for the Interaction between the Cytoplasmic Domain of the Hyaluronate Receptor Layilin and the Talin F3 Subdomain. Journal of Molecular Biology, 382(1), 112-126. doi:10.1016/j.jmb.2008.06.087

    DOI: 10.1016/j.jmb.2008.06.087

    NMR assignment of the C-terminal actin-binding domain of talin (Journal article)

    Goult, B. T., Gingras, A. R., Bate, N., Roberts, G. C. K., Critchley, D. R., & Barsukov, I. L. (2008). NMR assignment of the C-terminal actin-binding domain of talin. Biomolecular NMR Assignments, 2(1), 17-19. doi:10.1007/s12104-007-9073-5

    DOI: 10.1007/s12104-007-9073-5

    Crystal structure of the Ca2+-form and Ca2+-binding kinetics of metastasis-associated protein, S100A4 (Journal article)

    Gingras, A. R., Basran, J., Prescott, A., Kriajevska, M., Bagshaw, C. R., & Barsukov, I. L. (2008). Crystal structure of the Ca2+-form and Ca2+-binding kinetics of metastasis-associated protein, S100A4. FEBS Letters, 582(12), 1651-1656. doi:10.1016/j.febslet.2008.04.017

    DOI: 10.1016/j.febslet.2008.04.017

    The structure of the C-terminal actin-binding domain of talin (Journal article)

    Gingras, A. R., Bate, N., Goult, B. T., Hazelwood, L., Canestrelli, I., Grossmann, J. G., . . . Critchley, D. R. (2008). The structure of the C-terminal actin-binding domain of talin. The EMBO Journal, 27(2), 458-469. doi:10.1038/sj.emboj.7601965

    DOI: 10.1038/sj.emboj.7601965

    The structure of the C-terminal actin-binding domain of talin (Journal article)

    Gingras, A. R., Bate, N., Goult, B. J., Hazelwood, L., Canestrelli, I., Grossmann, J. G., . . . Critchley, D. R. (2008). The structure of the C-terminal actin-binding domain of talin. EMBO Journal, 27, 458-469.

    2006

    Role of vinculin in regulating focal adhesion turnover (Journal article)

    Saunders, R. M., Holt, M. R., Jennings, L., Sutton, D. H., Barsukov, I. L., Bobkov, A., . . . Critchley, D. R. (2006). Role of vinculin in regulating focal adhesion turnover. European Journal of Cell Biology, 85(6), 487-500. doi:10.1016/j.ejcb.2006.01.014

    DOI: 10.1016/j.ejcb.2006.01.014

    The Activity of the Vinculin Binding Sites in Talin Is Influenced by the Stability of the Helical Bundles That Make Up The Talin Rod (Journal article)

    Patel, B., Gingras, A. R., Bobkov, A. A., Fujimoto, L. M., Zhang, M., Liddington, R. C., . . . Critchley, D. R. (2006). The Activity of the Vinculin Binding Sites in Talin Is Influenced by the Stability of the Helical Bundles That Make Up The Talin Rod. Journal of Biological Chemistry, 281(11), 7458-7467. doi:10.1074/jbc.m508058200

    DOI: 10.1074/jbc.m508058200

    Structural and Dynamic Characterization of a Vinculin Binding Site in the Talin Rod†,‡ (Journal article)

    Gingras, A. R., Vogel, K. -P., Steinhoff, H. -J., Ziegler, W. H., Patel, B., Emsley, J., . . . Barsukov, I. L. (2006). Structural and Dynamic Characterization of a Vinculin Binding Site in the Talin Rod†,‡. Biochemistry, 45(6), 1805-1817. doi:10.1021/bi052136l

    DOI: 10.1021/bi052136l

    2005

    Mapping and Consensus Sequence Identification for Multiple Vinculin Binding Sites within the Talin Rod (Journal article)

    Gingras, A. R., Ziegler, W. H., Frank, R., Barsukov, I. L., Roberts, G. C. K., Critchley, D. R., & Emsley, J. (2005). Mapping and Consensus Sequence Identification for Multiple Vinculin Binding Sites within the Talin Rod. Journal of Biological Chemistry, 280(44), 37217-37224. doi:10.1074/jbc.m508060200

    DOI: 10.1074/jbc.m508060200

    Reaction of Morphinone Reductase with 2-Cyclohexen-1-one and 1-Nitrocyclohexene (Journal article)

    Messiha, H. L., Munro, A. W., Bruce, N. C., Barsukov, I., & Scrutton, N. S. (2005). Reaction of Morphinone Reductase with 2-Cyclohexen-1-one and 1-Nitrocyclohexene. Journal of Biological Chemistry, 280(11), 10695-10709. doi:10.1074/jbc.m410595200

    DOI: 10.1074/jbc.m410595200

    A Vinculin Binding Domain from the Talin Rod Unfolds to Form a Complex with the Vinculin Head (Journal article)

    Fillingham, I., Gingras, A. R., Papagrigoriou, E., Patel, B., Emsley, J., Critchley, D. R., . . . Barsukov, I. L. (2005). A Vinculin Binding Domain from the Talin Rod Unfolds to Form a Complex with the Vinculin Head. Structure, 13(1), 65-74. doi:10.1016/j.str.2004.11.006

    DOI: 10.1016/j.str.2004.11.006

    2004

    Activation of a vinculin-binding site in the talin rod involves rearrangement of a five-helix bundle (Journal article)

    Papagrigoriou, E., Gingras, A. R., Barsukov, I. L., Bate, N., Fillingham, I. J., Patel, B., . . . Emsley, J. (2004). Activation of a vinculin-binding site in the talin rod involves rearrangement of a five-helix bundle. The EMBO Journal, 23(15), 2942-2951. doi:10.1038/sj.emboj.7600285

    DOI: 10.1038/sj.emboj.7600285

    Atomic Resolution Structures and Solution Behavior of Enzyme-Substrate Complexes of Enterobacter cloacae PB2 Pentaerythritol Tetranitrate Reductase (Journal article)

    Khan, H., Barna, T., Harris, R. J., Bruce, N. C., Barsukov, I., Munro, A. W., . . . Scrutton, N. S. (2004). Atomic Resolution Structures and Solution Behavior of Enzyme-Substrate Complexes of Enterobacter cloacae PB2 Pentaerythritol Tetranitrate Reductase. Journal of Biological Chemistry, 279(29), 30563-30572. doi:10.1074/jbc.m403541200

    DOI: 10.1074/jbc.m403541200

    A stereodivergent asymmetric approach to difluorinated aldonic acids (Journal article)

    Audouard, C., Barsukov, I., Fawcett, J., Griffith, G. A., Percy, J. M., Pintat, S., & Smith, C. A. (n.d.). A stereodivergent asymmetric approach to difluorinated aldonic acids. Chem. Commun., (13), 1526-1527. doi:10.1039/b405067c

    DOI: 10.1039/b405067c

    2003

    Phosphatidylinositol Phosphate Kinase Type 1? and ß1-Integrin Cytoplasmic Domain Bind to the Same Region in the Talin FERM Domain (Journal article)

    Barsukov, I. L., Prescot, A., Bate, N., Patel, B., Floyd, D. N., Bhanji, N., . . . Critchley, D. R. (2003). Phosphatidylinositol Phosphate Kinase Type 1? and ß1-Integrin Cytoplasmic Domain Bind to the Same Region in the Talin FERM Domain. Journal of Biological Chemistry, 278(33), 31202-31209. doi:10.1074/jbc.m303850200

    DOI: 10.1074/jbc.m303850200

    The Inhibitor Thiomandelic Acid Binds to Both Metal Ions in Metallo-ß-lactamase and Induces Positive Cooperativity in Metal Binding (Journal article)

    Damblon, C., Jensen, M., Ababou, A., Barsukov, I., Papamicael, C., Schofield, C. J., . . . Roberts, G. C. K. (2003). The Inhibitor Thiomandelic Acid Binds to Both Metal Ions in Metallo-ß-lactamase and Induces Positive Cooperativity in Metal Binding. Journal of Biological Chemistry, 278(31), 29240-29251. doi:10.1074/jbc.m301562200

    DOI: 10.1074/jbc.m301562200

    2002

    Further characterization of the interaction between the cytoskeletal proteins talin and vinculin (Journal article)

    BASS, M. D., PATEL, B., BARSUKOV, I. G., FILLINGHAM, I. J., MASON, R., SMITH, B. J., . . . CRITCHLEY, D. R. (2002). Further characterization of the interaction between the cytoskeletal proteins talin and vinculin. Biochemical Journal, 362(3), 761-768. doi:10.1042/bj3620761

    DOI: 10.1042/bj3620761

    2001

    Crystal structure of pentaerythritol tetranitrate reductase: “flipped” binding geometries for steroid substrates in different redox states of the enzyme (Journal article)

    Barna, T. M., Khan, H., Bruce, N. C., Barsukov, I., Scrutton, N. S., & Moody, P. C. E. (2001). Crystal structure of pentaerythritol tetranitrate reductase: “flipped” binding geometries for steroid substrates in different redox states of the enzyme. Journal of Molecular Biology, 310(2), 433-447. doi:10.1006/jmbi.2001.4779

    DOI: 10.1006/jmbi.2001.4779

    Structural consequences of site-directed mutagenesis in flexible protein domains: NMR characterization of the L(55,56)S mutant of RhoGDI (Journal article)

    Golovanov, A. P., Hawkins, D., Barsukov, I., Badii, R., Bokoch, G. M., Lian, L. Y., & Roberts, G. C. (2001). Structural consequences of site-directed mutagenesis in flexible protein domains: NMR characterization of the L(55,56)S mutant of RhoGDI. Eur J Biochem, 268(8), 2253-2260. Retrieved from http://www.ncbi.nlm.nih.gov/

    Structure-activity relationships in flexible protein domains: regulation of rho GTPases by RhoGDI and D4 GDI (Journal article)

    Golovanov, A. P., Chuang, T. -H., DerMardirossian, C., Barsukov, I., Hawkins, D., Badii, R., . . . Roberts, G. C. K. (2001). Structure-activity relationships in flexible protein domains: regulation of rho GTPases by RhoGDI and D4 GDI. Journal of Molecular Biology, 305(1), 121-135. doi:10.1006/jmbi.2000.4262

    DOI: 10.1006/jmbi.2000.4262

    2000

    Mapping the binding site for the GTP-binding protein Rac-1 on its inhibitor RhoGDI-1 (Journal article)

    Lian, L. Y., Barsukov, I., Golovanov, A. P., Hawkins, D. I., Badii, R., Sze, K. H., . . . Roberts, G. C. (2000). Mapping the binding site for the GTP-binding protein Rac-1 on its inhibitor RhoGDI-1. Structure, 8(1), 47-55. Retrieved from http://www.ncbi.nlm.nih.gov/

    1997

    1H, 15N and 13C NMR resonance assignment, secondary structure and global fold of the FMN-binding domain of human cytochrome P450 reductase (Journal article)

    Barsukov, I., Modi, S., Lian, L. Y., Sze, K. H., Paine, M. J., Wolf, C. R., & Roberts, G. C. (1997). 1H, 15N and 13C NMR resonance assignment, secondary structure and global fold of the FMN-binding domain of human cytochrome P450 reductase. J Biomol NMR, 10(1), 63-75. Retrieved from http://www.ncbi.nlm.nih.gov/

    1996

    The Conformation of Coenzyme A Bound to Chloramphenicol Acetyltransferase Determined by Transferred NOE Experiments (Journal article)

    Barsukov, I. L., Lian, L. -Y., Ellis, J., Sze, K. -H., Shaw, W. V., & Roberts, G. C. K. (1996). The Conformation of Coenzyme A Bound to Chloramphenicol Acetyltransferase Determined by Transferred NOE Experiments. Journal of Molecular Biology, 262(4), 543-558. doi:10.1006/jmbi.1996.0534

    DOI: 10.1006/jmbi.1996.0534

    1995

    Role of the Active-Site Carboxylate in Dihydrofolate Reductase: Kinetic and Spectroscopic Studies of the Aspartate 26 .fwdarw. Asparagine Mutant of the Lactobacillus casei Enzyme (Journal article)

    Basran, J., Casarotto, M. G., Barsukov, I. L., & Roberts, G. C. K. (1995). Role of the Active-Site Carboxylate in Dihydrofolate Reductase: Kinetic and Spectroscopic Studies of the Aspartate 26 .fwdarw. Asparagine Mutant of the Lactobacillus casei Enzyme. Biochemistry, 34(9), 2872-2882. doi:10.1021/bi00009a018

    DOI: 10.1021/bi00009a018

    1994

    Protein-ligand interactions: exchange processes and determination of ligand conformation and protein-ligand contacts (Journal article)

    Lian, L. Y., Barsukov, I. L., Sutcliffe, M. J., Sze, K. H., & Roberts, G. C. (1994). Protein-ligand interactions: exchange processes and determination of ligand conformation and protein-ligand contacts. Methods Enzymol, 239, 657-700. Retrieved from http://www.ncbi.nlm.nih.gov/