Photo of Dr Philip Brownridge

Dr Philip Brownridge

Research Associate Biochemistry & Systems Biology

Publications

2021

Temporal modulation of the NF-?B RelA network in response to different types of DNA damage. (Journal article)

Campbell, A. E., Ferraz Franco, C., Su, L. -I., Corbin, E. K., Perkins, S., Kalyuzhnyy, A., . . . Eyers, C. E. (2021). Temporal modulation of the NF-?B RelA network in response to different types of DNA damage.. The Biochemical journal, 478(3), 533-551. doi:10.1042/bcj20200627

DOI: 10.1042/bcj20200627

2020

CSF Levels of Elongation Factor Tu Is Associated With Increased Mortality in Malawian Adults With Streptococcus pneumoniae Meningitis (Journal article)

Wall, E. C., Brownridge, P., Laing, G., Terra, V. S., Mlozowa, V., Denis, B., . . . Brown, J. S. (2020). CSF Levels of Elongation Factor Tu Is Associated With Increased Mortality in Malawian Adults With Streptococcus pneumoniae Meningitis. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 10. doi:10.3389/fcimb.2020.603623

DOI: 10.3389/fcimb.2020.603623

Social status and ejaculate composition in the house mouse (Journal article)

Bayram, H., Franco, C., Brownridge, P., Claydon, A., Koch, N., Hurst, J., . . . Stockley, P. (2020). Social status and ejaculate composition in the house mouse. Philosophical Transactions of the Royal Society B: Biological Sciences, 375(1813). doi:10.1098/rstb.2020.0083

DOI: 10.1098/rstb.2020.0083

Oxygen-dependent changes in HIF binding partners and post-translational modifications regulate stability and transcriptional activity (Journal article)

Daly, L., Brownridge, P., Sée, V., & Eyers, C. (2020). Oxygen-dependent changes in HIF binding partners and post-translational modifications regulate stability and transcriptional activity. doi:10.1101/2020.11.12.379768

DOI: 10.1101/2020.11.12.379768

Revealing mechanisms of mating plug function under sexual selection (Journal article)

Stockley, P., Franco, C., Claydon, A., Davidson, A., Hammond, D., Brownridge, P., . . . Beynon, R. (2020). Revealing mechanisms of mating plug function under sexual selection. Proceedings of the National Academy of Sciences of USA, 117(44), 27465-27473. doi:10.1073/pnas.1920526117

DOI: 10.1073/pnas.1920526117

Characterisation of protease activity during SARS-CoV-2 infection identifies novel viral cleavage sites and cellular targets for drug repurposing (Journal article)

Meyer, B., Chiaravalli, J., Gellenoncourt, S., Brownridge, P., Bryne, D., Daly, L., . . . Emmott, E. (2020). Characterisation of protease activity during SARS-CoV-2 infection identifies novel viral cleavage sites and cellular targets for drug repurposing. doi:10.1101/2020.09.16.297945

DOI: 10.1101/2020.09.16.297945

Characterisation of protease activity during SARS-CoV-2 infection identifies novel viral cleavage sites and cellular targets for drug repurposing (Journal article)

Meyer, B., Chiaravalli, J., Brownridge, P., Bryne, D., Daly, L., Agou, F., . . . Emmott, E. (n.d.). Characterisation of protease activity during SARS-CoV-2 infection identifies novel viral cleavage sites and cellular targets for drug repurposing. bioRxiv. doi:10.1101/2020.09.16.297945v1

DOI: 10.1101/2020.09.16.297945v1

PEPPI-MS: Polyacrylamide-Gel-Based Prefractionation for Analysis of Intact Proteoforms and Protein Complexes by Mass Spectrometry (Journal article)

Takemori, A., Butcher, D. S., Harman, V. M., Brownridge, P., Shima, K., Higo, D., . . . Takemori, N. (2020). PEPPI-MS: Polyacrylamide-Gel-Based Prefractionation for Analysis of Intact Proteoforms and Protein Complexes by Mass Spectrometry. JOURNAL OF PROTEOME RESEARCH, 19(9), 3779-3791. doi:10.1021/acs.jproteome.0c00303

DOI: 10.1021/acs.jproteome.0c00303

Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signalling networks using SILAC-based phosphoproteomics (Journal article)

Byrne, D. P., Clarke, C. J., Brownridge, P. J., Kalyuzhnyy, A., Perkins, S., Campbell, A., . . . Eyers, C. E. (2020). Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signalling networks using SILAC-based phosphoproteomics. The Biochemical journal, 477(13), 2451-2475. doi:10.1042/bcj20200309

DOI: 10.1042/bcj20200309

Decoding the stoichiometric composition and organisation of bacterial metabolosomes (Journal article)

Yang, M., Simpson, D., Wenner, N., Brownridge, P., Harman, V., Hinton, J., . . . Liu, L. (2020). Decoding the stoichiometric composition and organisation of bacterial metabolosomes. Nature Communications, 11(1). doi:10.1038/s41467-020-15888-4

DOI: 10.1038/s41467-020-15888-4

Quantitative Proteomics of Enriched Esophageal and Gut Tissues from the Human Blood Fluke Schistosoma mansoni Pinpoints Secreted Proteins for Vaccine Development (Journal article)

Neves, L. X., Wilson, R. A., Brownridge, P., Harman, V. M., Holman, S. W., Beynon, R. J., . . . Castro-Borges, W. (2020). Quantitative Proteomics of Enriched Esophageal and Gut Tissues from the Human Blood Fluke Schistosoma mansoni Pinpoints Secreted Proteins for Vaccine Development. JOURNAL OF PROTEOME RESEARCH, 19(1), 314-326. doi:10.1021/acs.jproteome.9b00531

DOI: 10.1021/acs.jproteome.9b00531

Large scale and significant expression from pseudogenes in Sodalis glossinidius - a facultative bacterial endosymbiont (Journal article)

Darby, A. C., Goodhead, I., Blow, F., Brownridge, P., Hughes, M., Kenny, J., . . . Beynon, R. (2020). Large scale and significant expression from pseudogenes in Sodalis glossinidius - a facultative bacterial endosymbiont. Microbial Genomics, 6(1), 3-15. doi:10.1099/mgen.0.000285

DOI: 10.1099/mgen.0.000285

2019

The heparin-binding proteome in normal pancreas and murine experimental acute pancreatitis (Internet publication)

Nunes, Q. M., Dunhao, S., Brownridge, P. J., Simpson, D. M., Sun, C., Li, Y., . . . Fernig, D. G. (2019). The heparin-binding proteome in normal pancreas and murine experimental acute pancreatitis. doi:10.1101/497271

DOI: 10.1101/497271

Strong anion exchange-mediated phosphoproteomics reveals extensive human non-canonical phosphorylation (Journal article)

Hardman, G., Perkins, S., Brownridge, P., Clarke, C., Byrne, D., Campbell, A., . . . Eyers, C. (2019). Strong anion exchange-mediated phosphoproteomics reveals extensive human non-canonical phosphorylation. The EMBO Journal, 38(21). doi:10.15252/embj.2018100847

DOI: 10.15252/embj.2018100847

The heparin-binding proteome in normal pancreas and murine experimental acute pancreatitis (Journal article)

Nunes, Q. M., Su, D., Brownridge, P. J., Simpson, D. M., Sun, C., Li, Y., . . . Fernig, D. G. (2019). The heparin-binding proteome in normal pancreas and murine experimental acute pancreatitis. PLOS ONE, 14(6). doi:10.1371/journal.pone.0217633

DOI: 10.1371/journal.pone.0217633

Adding function to the genome of African Salmonella Typhimurium ST313 strain D23580 (Journal article)

Canals, R., Hammarlöf, D. L., Kröger, C., Owen, S. V., Fong, W. Y., Lacharme-Lora, L., . . . Hinton, J. C. D. (2019). Adding function to the genome of African Salmonella Typhimurium ST313 strain D23580. PLoS Biology, 17(1). doi:10.1371/journal.pbio.3000059

DOI: 10.1371/journal.pbio.3000059

Adding function to the genome of AfricanSalmonellaST313 (Journal article)

Canals, R., Hammarlöf, D., Kröger, C., Owen, S., Fong, W. Y., Lacharme-Lora, L., . . . Hinton, J. (2019). Adding function to the genome of AfricanSalmonellaST313. None (preprint), 17(1). doi:10.1101/450098

DOI: 10.1101/450098

2018

The Heparin-Binding Proteome in Murine Experimental Acute Pancreatitis (Journal article)

Nunes, Q. M., Su, D., Brownridge, P. J., Sun, C., Li, Y., Huang, W., . . . Fernig, D. G. (2016). The Heparin-Binding Proteome in Murine Experimental Acute Pancreatitis. PANCREAS, 45(10), 1530. Retrieved from http://gateway.webofknowledge.com/

2017

Evaluation of Parameters for Confident Phosphorylation Site Localization using an Orbitrap Fusion Tribrid Mass Spectrometer (Journal article)

Ferries., Perkins, S., Brownridge., Campbell, A., Eyers, P., Jones, A., & Eyers, C. E. (2017). Evaluation of Parameters for Confident Phosphorylation Site Localization using an Orbitrap Fusion Tribrid Mass Spectrometer. Journal of Proteome Research, 16(9), 3448-3459. doi:10.1021/acs.jproteome.7b00337

DOI: 10.1021/acs.jproteome.7b00337

DOSCATs: Double standards for protein quantification (Journal article)

Bennett, R. J., Simpson, D. M., Holman, S. W., Ryan, S., Brownridge, P., Eyers, C. E., . . . Beynon, R. J. (2017). DOSCATs: Double standards for protein quantification. Scientific Reports, 7. doi:10.1038/srep45570

DOI: 10.1038/srep45570

2016

cAMP-dependent protein kinase (PKA) complexes probed by complementary differential scanning fluorimetry and ion mobility-mass spectrometry (Journal article)

Byrne, D. P., Vonderach, M., Ferries, S., Brownridge, P. J., Eyers, C. E., & Eyers, P. A. (2016). cAMP-dependent protein kinase (PKA) complexes probed by complementary differential scanning fluorimetry and ion mobility-mass spectrometry. BIOCHEMICAL JOURNAL, 473, 3159-3175. doi:10.1042/BCJ20160648

DOI: 10.1042/BCJ20160648

Cross-species proteomics in analysis of mammalian sperm proteins (Journal article)

Bayram, H., Claydon, A., Brownridge, P., Hurst, J., Mileham, A., Stockley, P., . . . Hammond, D. (2016). Cross-species proteomics in analysis of mammalian sperm proteins. Journal of Proteomics, 135, 38-50. doi:10.1016/j.jprot.2015.12.027

DOI: 10.1016/j.jprot.2015.12.027

Direct and Absolute Quantification of over 1800 Yeast Proteins via Selected Reaction Monitoring (Journal article)

Lawless, C., Holman, S. W., Brownridge, P., Lanthaler, K., Harman, V. M., Watkins, R., . . . Hubbard, S. J. (2016). Direct and Absolute Quantification of over 1800 Yeast Proteins via Selected Reaction Monitoring. Molecular and Cellular Proteomics, 15(4), 1309-1322. doi:10.1074/mcp.M115.054288

DOI: 10.1074/mcp.M115.054288

2015

Sperm competition risk drives plasticity in seminal fluid composition (Journal article)

Ramm, S., Edward, D., Claydon, A., Hammond, D., Brownridge, P., Hurst, J., . . . Stockley, P. (2015). Sperm competition risk drives plasticity in seminal fluid composition. BMC Biology, 13. doi:10.1186/s12915-015-0197-2

DOI: 10.1186/s12915-015-0197-2

Redox proteomic analysis of the gastrocnemius muscle from adult and old mice. (Journal article)

McDonagh, B., Sakellariou, G. K., Smith, N. T., Brownridge, P., & Jackson, M. J. (2015). Redox proteomic analysis of the gastrocnemius muscle from adult and old mice.. Data in brief, 4, 344-348. doi:10.1016/j.dib.2015.06.012

DOI: 10.1016/j.dib.2015.06.012

Redox proteomic analysis of the gastrocnemius muscle from adult and old mice. (Journal article)

McDonagh, B., Sakellariou, Y., Smith, N., Brownridge, P., & Jackson, M. (n.d.). Redox proteomic analysis of the gastrocnemius muscle from adult and old mice.. Redox proteomic analysis of the gastrocnemius muscle from adult and old mice..

2014

Development of a Method for Absolute Quantification of Equine Acute Phase Proteins Using Concatenated Peptide Standards and Selected Reaction Monitoring (Journal article)

Bundgaard, L., Jacobsen, S., Dyrlund, T. F., Sorensen, M. A., Harman, V. M., Beynon, R. J., . . . Bendixen, E. (2014). Development of a Method for Absolute Quantification of Equine Acute Phase Proteins Using Concatenated Peptide Standards and Selected Reaction Monitoring. JOURNAL OF PROTEOME RESEARCH, 13(12), 5635-5647. doi:10.1021/pr500607s

DOI: 10.1021/pr500607s

Differential Cysteine Labeling and Global Label-Free Proteomics Reveals an Altered Metabolic State in Skeletal Muscle Aging (Journal article)

McDonagh, B., Sakellariou, G. K., Smith, N. T., Brownridge, P., & Jackson, M. J. (2014). Differential Cysteine Labeling and Global Label-Free Proteomics Reveals an Altered Metabolic State in Skeletal Muscle Aging. Journal of Proteome Research, 13(11), 5008-5021. doi:10.1021/pr5006394

DOI: 10.1021/pr5006394

Differential cysteine labeling and global label-free proteomics reveals an altered metabolic state in skeletal muscle aging (Journal article)

McDonagh, B., Sakellariou, G., Smith, N., Brownridge, P., & Jackson, M. (2014). Differential cysteine labeling and global label-free proteomics reveals an altered metabolic state in skeletal muscle aging. Journal of Proteome Research, 13(11), 5008-5021. doi:10.1021/pr5006394

DOI: 10.1021/pr5006394

Absolute Quantification of over 1900 Yeast Proteins Provide Insights into Translational Control and Protein Dynamics (Journal article)

Lawless, C., Holman, S. W., Brownridge, P., Lanthaler1, K., Harman, V. M., Watkins, R., . . . Hubbard, S. J. (2014). Absolute Quantification of over 1900 Yeast Proteins Provide Insights into Translational Control and Protein Dynamics.

2013

Quantitative analysis of chaperone network throughput in budding yeast (Journal article)

Brownridge, P., Lawless, C., Payapilly, A. B., Lanthaler, K., Holman, S. W., Harman, V. M., . . . Hubbard, S. J. (2013). Quantitative analysis of chaperone network throughput in budding yeast. PROTEOMICS, 13(8), 1276-1291. doi:10.1002/pmic.201200412

DOI: 10.1002/pmic.201200412

2012

A Software Toolkit and Interface for Performing Stable Isotope Labeling and Top3 Quantification Using Progenesis LC-MS (Journal article)

Qi, D., Brownridge, P., Xia, D., Mackay, K., Gonzalez-Galarza, F. F., Kenyani, J., . . . Jones, A. R. (2012). A Software Toolkit and Interface for Performing Stable Isotope Labeling and Top3 Quantification Using Progenesis LC-MS. OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY, 16(9), 489-495. doi:10.1089/omi.2012.0042

DOI: 10.1089/omi.2012.0042

Absolute multiplexed protein quantification using QconCAT technology. (Journal article)

Brownridge, P. J., Harman, V. M., Simpson, D. M., & Beynon, R. J. (2012). Absolute multiplexed protein quantification using QconCAT technology.. Methods in molecular biology (Clifton, N.J.), 893, 267-293. doi:10.1007/978-1-61779-885-6_18

DOI: 10.1007/978-1-61779-885-6_18

A proteomics strategy for determining the synthesis and degradation rates of individual proteins in fish (Journal article)

Doherty, M. K., Brownridge, P., Owen, M. A. G., Davies, S. J., Young, I. S., & Whitfield, P. D. (2012). A proteomics strategy for determining the synthesis and degradation rates of individual proteins in fish. JOURNAL OF PROTEOMICS, 75(14), 4471-4477. doi:10.1016/j.jprot.2012.03.025

DOI: 10.1016/j.jprot.2012.03.025

2011

Absolute Quantification of the Glycolytic Pathway in Yeast: DEPLOYMENT OF A COMPLETE QconCAT APPROACH (Journal article)

Carroll, K. M., Simpson, D. M., Eyers, C. E., Knight, C. G., Brownridge, P., Dunn, W. B., . . . Beynon, R. J. (2011). Absolute Quantification of the Glycolytic Pathway in Yeast: DEPLOYMENT OF A COMPLETE QconCAT APPROACH. MOLECULAR & CELLULAR PROTEOMICS, 10(12). doi:10.1074/mcp.M111.007633

DOI: 10.1074/mcp.M111.007633

Global absolute quantification of a proteome: Challenges in the deployment of a QconCAT strategy (Journal article)

Brownridge, P., Holman, S. W., Gaskell, S. J., Grant, C. M., Harman, V. M., Hubbard, S. J., . . . Beynon, R. J. (2011). Global absolute quantification of a proteome: Challenges in the deployment of a QconCAT strategy. PROTEOMICS, 11(15), 2957-2970. doi:10.1002/pmic.201100039

DOI: 10.1002/pmic.201100039

The importance of the digest: Proteolysis and absolute quantification in proteomics (Journal article)

Brownridge, P., & Beynon, R. J. (2011). The importance of the digest: Proteolysis and absolute quantification in proteomics. METHODS, 54(4), 351-360. doi:10.1016/j.ymeth.2011.05.005

DOI: 10.1016/j.ymeth.2011.05.005

Absolute Multiplexed Protein Quantification Using QconCAT technology (Chapter)

Brownridge, P. J., Harman, V., Simpson, D. M., & Beynon, R. J. (2011). Absolute Multiplexed Protein Quantification Using QconCAT technology. In K. Marcus (Ed.), Quantitative Methods in Proteomics (pp. 100-115). New York: Humana Press.

Absolute Quantification of the Glycolytic Pathway in Yeast: DEPLOYMENT OF A COMPLETE QconCAT APPROACH (Journal article)

Carroll, K. M., Simpson, D. M., Eyers, C. E., Knight, C. G., Brownridge, P., Dunn, W. B., . . . Beynon, R. J. (2011). Absolute Quantification of the Glycolytic Pathway in Yeast: DEPLOYMENT OF A COMPLETE QconCAT APPROACH. Molecular & Cellular Proteomics, 10(12). doi:10.1074/mcp.M111.007633-1

DOI: 10.1074/mcp.M111.007633-1

Alternative routes to absolute protein quantification (Conference Paper)

Brownridge, P. J., Harman, V. M., Lawless, C., Lanthaler, K., Watkins, R., Holman, S. W., . . . Beynon, R. J. (2011). Alternative routes to absolute protein quantification. In 8th BSPR-EBI Meeting 'From the visible to the hidden proteome' (pp. Poster). Cambridge, UK: BSPR-EBI Meeting.

Large scale production of QconCAT standards for quantitative proteomics (Conference Paper)

Harman, V., Watkins, R. H., Brownridge, P., Holman, S. W., Lanthaler, K., Lawless, C., . . . Beynon, R. (2011). Large scale production of QconCAT standards for quantitative proteomics. In 8th BSPR-EBI Meeting 'From the visible to the hidden proteome' (pp. Poster). Cambridge, UK: BSPR-EBI Meeting.

2010

Degradative proteomics and disease mechanisms (Journal article)

Lanucara, F., Brownridge, P., Young, I. S., Whitfield, P. D., & Doherty, M. K. (2010). Degradative proteomics and disease mechanisms. PROTEOMICS CLINICAL APPLICATIONS, 4(2), 133-142. doi:10.1002/prca.200900159

DOI: 10.1002/prca.200900159

2009

Regional variation in parvalbumin isoform expression correlates with muscle performance in common carp (Cyprinus carpio) (Journal article)

Brownridge, P., de Mello, L. V., Peters, M., McLean, L., Claydon, A., Cossins, A. R., . . . Young, I. S. (2009). Regional variation in parvalbumin isoform expression correlates with muscle performance in common carp (Cyprinus carpio). JOURNAL OF EXPERIMENTAL BIOLOGY, 212(2), 184-193. doi:10.1242/jeb.021857

DOI: 10.1242/jeb.021857

2008

Integrating proteomics with physiology: The influence of parvalbumin isoforms on fish muscle performance (Journal article)

Brownridge, P., Peters, M., de Mello, L. V., Cossins, A. R., Whitfield, P. D., & Young, I. S. (2008). Integrating proteomics with physiology: The influence of parvalbumin isoforms on fish muscle performance. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 150(3), S75-S76. doi:10.1016/j.cbpa.2008.04.113

DOI: 10.1016/j.cbpa.2008.04.113