Proteolysis
Unkind cuts - proteolysis and proteomics
Brownridge, P. & Beynon, R.J. (2011) The importance of the digest: proteolysis and absolute quantification in proteomics. Methods, [PUBMED] [PDF] [Abstract...]
Limited proteolysis of native proteins
Ellison, D., Hinton, J., Hubbard, S.J. & Beynon, R.J. (1995) Limited proteolysis of native proteins: The interaction between avidin and proteinase K. Protein Science 4, 1337-1345 [PUBMED] [PDF] [Abstract...]
MMP zymography
Coughlan, A. D., Carter, S. D., Robertson, D. H. L., Kelly, D. F., Bennett, D., May, C. & Beynon, R. J. (1995). Zymographic analysis of synovial fluid metalloproteinases in canine arthritis. Vet. Comp. Orthopaed. Traum. 8, 62. [PUBMED] [PDF] [Abstract...]
Limited proteolysis of native proteins
Hubbard, S.J., Beynon, R.J. & Thornton, J.M. (1998) Assessment of conformational parameters as predictors of limited proteolytic sites in native protein structures. Prot. Eng. 11, 349-359 [PUBMED] [PDF] [Abstract...]
MMP Zymography
Coughlan, A. R., Robertson, D. H. L., Burke, R., Beynon, R.J., Carter, S. D. (1998) Isolation and identification of canine matrix metalloproteinase-2 (MMP-2). Vet J. 155, 231-237 [PUBMED] [PDF] [Abstract...]
Limited proteolysis of MUPs
Wu, C., Robertson, D.H.L., Hubbard, S.J. Gaskell, S.J. & Beynon, R.J. (1999) Proteolysis of native enzymes: Trapping of a reaction intermediate. J. Biol. Chem. 274: 1108-1115 [PUBMED] [PDF] [Abstract...]
How to suppress unwanted proteolyis
Beynon, R.J & Oliver, S. (1996) Prevention of unwanted proteolysis, in "Protein Purification Protocols" (Doonan, S., ed) Humana Press. pp 81-102 [Abstract...]
Review of thermolysins
Beynon, R.J. & Beaumont, A. (1998) M4 proteases: thermolysins. in "Handbook of Proteolytic Enzymes" (Barrett, A.J. Rawlings, N. & Woessner, F., eds) Academic press 1037-1046 [Abstract...]
Review of M4 proteases
Beynon, R.J. & Beaumont, A. (1998) M4 proteases: bacillolysins. in "Handbook of Proteolytic Enzymes" (Barrett, A.J. Rawlings, N. & Woessner, F., eds) Academic press 1046-1050 [Abstract...]
Canine MMP identification
Coughlan AR, Robertson DH, Burke R, Beynon RJ, Carter SD. (2000) Isolation and identification of canine matrix metalloproteinase-2 (MMP-2). Vet J. 155:231-237 [PUBMED] [PDF] [Abstract...]
Limited proteolysis
Hubbard, S. J. & Beynon, R. J. (2001). Proteolysis of native proteins as a structural probe. In Proteolytic Enzymes. A Practical Approach (Beynon, R. J. & Bond, J. S., eds.), pp. 233-264. OUP, Oxford. [Abstract...]
Protease inhibitors
Beynon, R. J. & Salvesen, G. (2001). Commercially available proteinase inhibitors. In Proteolytic Enzymes. A Practical Approach (Beynon, R. J. & Bond, J. S., eds.), pp. 317-330. OUP, Oxford. [Abstract...]
Sequential exoproteolysis is not valid as a structural probe
Beynon, R.J. (2004) Sequential exoproteolysis as a structural probe: a cautionary note. J. Mass. Spec. 39, 188-192. [PUBMED] [PDF] [Abstract...]
Prevention of proteolysis
Beynon RJ, Oliver S. (2004) Avoidance of proteolysis in extracts. Methods Mol Biol. 244:75-84. [PUBMED] [PDF] [Abstract...]
Peptide map drawing tool
Beynon, R.J. (2005) A simple tool for drawing proteolytic peptide maps. Bioinformatics 21, 674-675 [PUBMED] [PDF] [Abstract...]