Themes | Centre for Proteome Research, Liverpool

Proteome dynamics We were the first group to address the importance of proteome dynamics. We maintain that a proteome is not fully characterised until we can explain changes in protein content in the cell in terms of the rates of the opposing processes of protein synthesis and protein degradation. We continue to develop the concepts and methodologies to define proteome dynamics.

Protein characterisation Comparative proteomics assess the relative amounts of two proteins, but can attain this with relatively little information about the protein. However, there are many studies where we use the methodologies of protein chemistry to complete a detailed characterisation of a single purified protein - for example complete sequencing of lipocalins.

Proteomics methodology development Proteomics is a nascent field, and there is scope for developments in methodology, strategy and data analysis. We have a number of ongoing projects in proteomics development. Recent achievements include a new approach to absolute quantification (QconCAT) and a method for selective analysis of N-terminal peptides.

Protein characterisation Comparative proteomics assess the relative amounts of two proteins, but can attain this with relatively little information about the protein. However, there are many studies where we use the methodologies of protein chemistry to complete a detailed characterisation of a single purified protein - for example complete sequencing of lipocalins.

Comparative proteomics A significant part of our research programme emphasises the changes in protein expression that define two physiological or pathophysiological states. The pattern of protein expression is assessed by image analysis of gel electrophoretograms or by mass spectrometry, whether by stable isotope labelling or by the emergent label-free quantification methods.

Chemical communication We have a long standing collaboration with Prof Jane Hurst in the Mammalian Behaviour and Evolution Group [LINK]. By combining detailed molecular characterisation and sophisticated behavioural experiments, we are developing our understanding of the role of proteins in chemical signalling between individuals, and the modulation of this process in health and disease.