MMP Zymography

Coughlan, A. R., Robertson, D. H. L., Burke, R., Beynon, R.J., Carter, S. D. (1998) Isolation and identification of canine matrix metalloproteinase-2 (MMP-2). Vet J. 155, 231-237 [PUBMED] [PDF]

A canine gelatinase, with an apparent molecular mass of 62 kDa in non-reducing zymography, is produced by fibroblasts, chondrocytes and a myelomonocytic cell line. The enzyme has similar characteristics to human matrix metalloproteinase (MMP) 2 and cross-reacts in Western blotting analysis with a sheep polyclonal antiserum raised against human MMP-2. The 62 kDa canine protein was purified from cell culture media, and the N-terminal amino acid sequence determined following blotting on to a polyvinylidene difluoride (PVDF) membrane. The sequence was 87% identical to that published for human MMP-2. We therefore consider this enzyme to be canine MMP-2.