QconCAT
QconCAT for equine acute phase proteins
Bundgaard L, Jacobsen S, Dyrlund TF, Sørensen MA, Harman VM, Beynon RJ, Brownridge PJ, Petersen LJ, Bendixen E. (2014) Development of a method for absolute quantification of equine acute phase proteins using concatenated peptide standards and selected reaction monitoring. J Proteome Res. 2014 Sep 24. [Epub ahead of print]
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MUP complexity and analytical challenges
Beynon RJ, Armstrong SD, Gómez-Baena G, Lee V, Simpson D, Unsworth J, Hurst JL. (2014) The complexity of protein semiochemistry in mammals. Biochem Soc Trans. 2014 Aug 42:837-845 [PUBMED][PDF] [Abstract...]
Quantification of proteins in the human osteoarthritic secretome
Peffers MJ, Beynon RJ, Clegg PD. (2013) Absolute quantification of selected proteins in the human osteoarthritic secretome. Int J Mol Sci. 14(10):20658-81. doi: 10.3390/ijms141020658. [PDF][PUBMED] [Abstract...]
Quantitative proteomics in systems biology
Firczuk, H., Kannambath, S; Pahle, J; Claydon, Amy; Beynon, Robert, Duncan, John; Westerhoff, Hans; Mendes, Pedro & McCarthy, John (2013) An in vivo control map for the eukaryotic mRNA translation machinery. Mol. Sys. Biol 9: 635 [PDF] [PUBMED] [Abstract...]
Who's got the biggest workload? Analysis of chaperone abundance and workload
Brownridge P, Lawless C, Payapilly AB, Lanthaler K, Holman SW, Harman VM, Grant CM, Beynon RJ, Hubbard SJ. Quantitative analysis of chaperone network throughput in budding yeast (2012). Proteomics. 13(8) 1276-1291 [PUBMED][PDF] [Abstract...]
QconCATs for mastitis research
Bislev SL, Kusebauch U, Codrea MC, Beynon RJ, Harman VM, Røntved CM, Aebersold R, Moritz RL, Bendixen E. (2012) Quantotypic properties of QconCAT peptides targeting bovine host response to Streptococcus uberis. J Proteome Res. 11:1832-1843. [PUBMED][PDF] [Abstract...]
Designing QconCATs for a mammalian pathway
Simpson, D.M. & Beynon, R.J. (2012) QconCATs: design and expression of concatenated protein standards for multiplexed protein quantification. Analytical and Bioanalytical Chemistry 404: 977-989 [PUBMED][PDF] [Abstract...]
Quantification of a metabolic pathway
Carroll KM, Simpson DM, Eyers CE, Knight CG, Brownridge P, Dunn W, Winder CL, Lanthaler K, Pir P, Malys N, Kell DB, Oliver SG, Gaskell SJ, Beynon RJ. (2011) Absolute quantification of a metabolic pathway in yeast: deployment of a complete QconCAT approach. Mol Cell Proteomics. 2011 Sep 19. [PUBMED][PDF] [Abstract...]
Schistosome parasite QconCAT
Castro-Borgesa, W., Simpson, D.M., Dowlea, A., Curwen, R.S., Thomas-Oates, J., Beynon, R.J. & Wilson, A. R. (2011) Abundance of tegument surface proteins in the human blood fluke Schistosoma mansoni determined by QconCAT proteomics. J Proteomics [PUBMED][PDF] [Abstract...]
Unkind cuts - proteolysis and proteomics
Brownridge, P. & Beynon, R.J. (2011) The importance of the digest: proteolysis and absolute quantification in proteomics. Methods, [PUBMED] [PDF] [Abstract...]
Global proteome quantification: dreams or reality?
Brownridge, P., Holman, S.W., Gaskell, S.J., Grant, C., Harman, V.M., Lanthaler, K., Lawless, C., O’cualain, R., Sims, P., Watkins, R & Beynon, R.J. (2011) Global absolute quantification of a proteome: Challenges in the deployment of a QconCAT strategy. Proteomics, 11, 2957-2970 [PUBMED][PDF] [Abstract...]
Phosphorylation stoichiometry
Rigorous determination of the stoichiometry of protein phosphorylation using mass spectrometry (2009) Johnson H, Eyers CE, Eyers PA, Beynon RJ, Gaskell SJ. J Am Soc Mass Spectrom. 20, 2211-2220 [PUBMED][PDF] [Abstract...]
Designer genes for mass spectrometry
Eyers CE, Simpson DM, Wong SC, Beynon RJ, Gaskell SJ. (2008) QCAL-a Novel Standard for Assessing Instrument Conditions for Proteome Analysis. J Am Soc Mass Spectrom. 19, 1275-1280 [PUBMED][PDF] [Abstract...]
FTICR based quantification
Johnson, H., Wong, S C C, D M Simpson, R J Beynon, and S J Gaskell (2008) . Protein quantification by selective isolation and fragmentation of isotopic pairs using FT-ICR MS. J Am Soc Mass Spectrom 19, 973-977 [PUBMED][PDF] [Abstract...]
Absolute quantification by QconCAT
Rivers J, Simpson DM, Robertson DH, Gaskell SJ, Beynon RJ.(2007) Absolute multiplexed quantitative analysis of protein expression during muscle development using QconCAT. Mol Cell Proteomics. 6, 1416-1427. [PUBMED][PDF] [Abstract...]
QconCAT protocols defined
Pratt, J.M., Simpson, D.M., Doherty, M.K., Rivers, J, Gaskell, S.J. Beynon , R.J. (2006) Multiplexed absolute quantification for proteomics using concatenated signature peptides encoded by QconCAT genes. Nature Protocols 1, 1029 - 1043 [PUBMED][PDF] [Abstract...]
First paper describing QconCAT
Beynon, R.J., Doherty, M.K., Pratt, J.M. & Gaskell, S.J. (2005) Multiplexed absolute quantification in proteomics using artificial QCAT proteins of concatenated signature peptides rates. Nature Methods 2, 587-589 [PUBMED] [PDF [Abstract...]