2024
Chowdhury, S., Ricafrente, A., Cwiklinski, K., Sais, D., Dalton, J. P., Tran, N., & Donnelly, S. (2024). Exploring the utility of circulating miRNAs as diagnostic biomarkers of fasciolosis.. Scientific reports, 14(1), 7431. doi:10.1038/s41598-024-57704-9DOI: 10.1038/s41598-024-57704-9
2023
De Marco Verissimo, C., Cwiklinski, K., Nilsson, J., Mirgorodskaya, E., Jin, C., Karlsson, N. G., & Dalton, J. P. (2023). Glycan complexity and heterogeneity of glycoproteins in somatic extracts and secretome of the infective stage of the helminth Fasciola hepatica. Molecular and Cellular Proteomics, 100684. doi:10.1016/j.mcpro.2023.100684DOI: 10.1016/j.mcpro.2023.100684
Cwiklinski, K., McEvoy, A., Corrales, J. L., Jewhurst, H., Calvani, N. E. D., De Marco Verissimo, C., . . . Lalor, R. (2023). Fasciola hepatica antioxidant and protease-inhibitor cocktail recombinant vaccines administered five times elicit potent and sustained immune responses in sheep but do not confer protection. Veterinary Parasitology, 110049. doi:10.1016/j.vetpar.2023.110049DOI: 10.1016/j.vetpar.2023.110049
Corrales, J. L., McEvoy, A., Lalor, R., Cwiklinski, K., Doyle, S., Parkinson, M., . . . Dorey, A. L. (2023). The use of cathepsin L1 (FhCL1) serological ELISA in sentinel screening for liver fluke on sheep farms. Veterinary Parasitology: Regional Studies and Reports, 100924. doi:10.1016/j.vprsr.2023.100924DOI: 10.1016/j.vprsr.2023.100924
Beesley, N. J., Cwiklinski, K., Allen, K., Hoyle, R. C., Spithill, T. W., La Course, E. J., . . . Hodgkinson, J. E. (n.d.). A major locus confers triclabendazole resistance in Fasciola hepatica and shows dominant inheritance. PLOS Pathogens, 19(1), e1011081. doi:10.1371/journal.ppat.1011081DOI: 10.1371/journal.ppat.1011081
2022
Cwiklinski, K., & Dalton, J. P. (2022). Omics tools enabling vaccine discovery against fasciolosis. TRENDS IN PARASITOLOGY, 38(12), 1068-1079. doi:10.1016/j.pt.2022.09.009DOI: 10.1016/j.pt.2022.09.009
Cwiklinski, K., & Dalton, J. P. (2022). Exploiting Comparative Omics to Understand the Pathogenic and Virulence-Associated Protease: Anti-Protease Relationships in the Zoonotic Parasites <i>Fasciola hepatica</i> and <i>Fasciola gigantica</i>. GENES, 13(10). doi:10.3390/genes13101854DOI: 10.3390/genes13101854
Calvani, N. E. D., Verissimo, C. D. M., Jewhurst, H. L., Cwiklinski, K., Flaus, A., & Dalton, J. P. (2022). Two Distinct Superoxidase Dismutases (SOD) Secreted by the Helminth Parasite <i>Fasciola hepatica</i> Play Roles in Defence against Metabolic and Host Immune Cell-Derived Reactive Oxygen Species (ROS) during Growth and Development. ANTIOXIDANTS, 11(10). doi:10.3390/antiox11101968DOI: 10.3390/antiox11101968
De Marco Verissimo, C., López Corrales, J., Dorey, A. L., Cwiklinski, K., Lalor, R., Calvani, N. E. D., . . . Dalton, J. P. (2022). Production of a functionally active recombinant SARS-CoV-2 (COVID-19) 3C-like protease and a soluble inactive 3C-like protease-RBD chimeric in a prokaryotic expression system.. Epidemiology and infection, 150, e128. doi:10.1017/s0950268822001078DOI: 10.1017/s0950268822001078
Ricafrente, A., Cwiklinski, K., Nguyen, H., Dalton, J. P., Tran, N., & Donnelly, S. (2022). Stage-specific miRNAs regulate gene expression associated with growth, development and parasite-host interaction during the intra-mammalian migration of the zoonotic helminth parasite Fasciola hepatica. BMC Genomics, 23(1). doi:10.1186/s12864-022-08644-zDOI: 10.1186/s12864-022-08644-z
Cwiklinski, K., Drysdale, O., López Corrales, J., Corripio-Miyar, Y., De Marco Verissimo, C., Jewhurst, H., . . . Dalton, J. P. (2022). Targeting Secreted Protease/Anti-Protease Balance as a Vaccine Strategy against the Helminth <i>Fasciola hepatica</i>.. Vaccines, 10(2), 155. doi:10.3390/vaccines10020155DOI: 10.3390/vaccines10020155
De Marco Verissimo, C., Jewhurst, H. L., Dobó, J., Gál, P., Dalton, J. P., & Cwiklinski, K. (n.d.). Fasciola hepatica is refractory to complement killing by preventing attachment of mannose binding lectin (MBL) and inhibiting MBL-associated serine proteases (MASPs) with serpins. PLOS Pathogens, 18(1), e1010226. doi:10.1371/journal.ppat.1010226DOI: 10.1371/journal.ppat.1010226
2021
Lalor, R., Cwiklinski, K., Calvani, N. E. D., Dorey, A., Hamon, S., Corrales, J. L., . . . De Marco Verissimo, C. (2021). Pathogenicity and virulence of the liver flukes <i>Fasciola hepatica</i> and <i>Fasciola</i><i>Gigantica</i> that cause the zoonosis Fasciolosis.. Virulence, 12(1), 2839-2867. doi:10.1080/21505594.2021.1996520DOI: 10.1080/21505594.2021.1996520
The discovery of
<i>Fasciola hepatica</i>
and its life cycle. (Chapter)
Andrews, S. J., Cwiklinski, K., & Dalton, J. P. (2021). The discovery of <i>Fasciola hepatica</i> and its life cycle.. In Fasciolosis (pp. 1-22). CABI. doi:10.1079/9781789246162.0001DOI: 10.1079/9781789246162.0001
Applying 'omics' technologies to understand
<i>Fasciola</i>
spp. biology. (Chapter)
Cwiklinski, K., Verissimo, C. D. M., McVeigh, P., Donnelly, S., & Dalton, J. P. (2021). Applying 'omics' technologies to understand <i>Fasciola</i> spp. biology.. In Fasciolosis (pp. 338-378). CABI. doi:10.1079/9781789246162.0011DOI: 10.1079/9781789246162.0011
Barbour, T., Cwiklinski, K., Lalor, R., Dalton, J. P., & De Marco Verissimo, C. (2021). The Zoonotic Helminth Parasite <i>Fasciola hepatica</i><i>:</i> Virulence-Associated Cathepsin B and Cathepsin L Cysteine Peptidases Secreted by Infective Newly Excysted Juveniles (NEJ).. Animals : an open access journal from MDPI, 11(12), 3495. doi:10.3390/ani11123495DOI: 10.3390/ani11123495
Robinson, M. W., & Cwiklinski, K. (2021). Proteomics of Host-Helminth Interactions.. Pathogens (Basel, Switzerland), 10(10), 1317. doi:10.3390/pathogens10101317DOI: 10.3390/pathogens10101317
López Corrales, J., Cwiklinski, K., De Marco Verissimo, C., Dorey, A., Lalor, R., Jewhurst, H., . . . Dalton, J. P. (2021). Diagnosis of sheep fasciolosis caused by Fasciola hepatica using cathepsin L enzyme-linked immunosorbent assays (ELISA).. Veterinary parasitology, 298, 109517. doi:10.1016/j.vetpar.2021.109517DOI: 10.1016/j.vetpar.2021.109517
Pérez-Caballero, R., Martínez-Moreno, F. J., Corripio-Miyar, Y., McNeilly, T. N., Cwiklinski, K., Dalton, J. P., . . . Buffoni, L. (2021). Antigen-specific response of CD4+ T cells and hepatic lymph node cells to Fasciola hepatica-derived molecules at the early and late stage of the infection in sheep. Veterinary Research, 52(1). doi:10.1186/s13567-021-00963-5DOI: 10.1186/s13567-021-00963-5
Becerro-Recio, D., González-Miguel, J., Ucero, A., Sotillo, J., Martínez-Moreno, Á., Pérez-Arévalo, J., . . . Siles-Lucas, M. (2021). Recognition Pattern of the <i>Fasciola hepatica</i> Excretome/Secretome during the Course of an Experimental Infection in Sheep by 2D Immunoproteomics.. Pathogens (Basel, Switzerland), 10(6), 725. doi:10.3390/pathogens10060725DOI: 10.3390/pathogens10060725
De Marco Verissimo, C., O'Brien, C., López Corrales, J., Dorey, A., Cwiklinski, K., Lalor, R., . . . Doyle, S. (2021). Improved diagnosis of SARS-CoV-2 by using nucleoprotein and spike protein fragment 2 in quantitative dual ELISA tests.. Epidemiology and infection, 149, e140. doi:10.1017/s0950268821001308DOI: 10.1017/s0950268821001308
Vorel, J., Cwiklinski, K., Roudnicky, P., Ilgova, J., Jedlickova, L., Dalton, J. P., . . . Kasny, M. (2021). <i>Eudiplozoon nipponicum</i> (Monogenea, Diplozoidae) and its adaptation to haematophagy as revealed by transcriptome and secretome profiling. BMC GENOMICS, 22(1). doi:10.1186/s12864-021-07589-zDOI: 10.1186/s12864-021-07589-z
Tran, N., Ricafrente, A., To, J., Lund, M., Marques, T. M., Gama-Carvalho, M., . . . Donnelly, S. (2021). Fasciola hepatica hijacks host macrophage miRNA machinery to modulate early innate immune responses.. Scientific reports, 11(1), 6712. doi:10.1038/s41598-021-86125-1DOI: 10.1038/s41598-021-86125-1
Mathew, R., Wunderlich, J., Thivierge, K., Cwiklinski, K., Dumont, C., Tilley, L., . . . Dalton, J. P. (2021). Biochemical and cellular characterisation of the Plasmodium falciparum M1 alanyl aminopeptidase (PfM1AAP) and M17 leucyl aminopeptidase (PfM17LAP).. Scientific reports, 11(1), 2854. doi:10.1038/s41598-021-82499-4DOI: 10.1038/s41598-021-82499-4
Cwiklinski, K., Robinson, M. W., Donnelly, S., & Dalton, J. P. (2021). Complementary transcriptomic and proteomic analyses reveal the cellular and molecular processes that drive growth and development of Fasciola hepatica in the host liver.. BMC genomics, 22(1), 46. doi:10.1186/s12864-020-07326-yDOI: 10.1186/s12864-020-07326-y
Dorey, A., Cwiklinski, K., Rooney, J., De Marco Verissimo, C., López Corrales, J., Jewhurst, H., . . . Lalor, R. (n.d.). Autonomous Non Antioxidant Roles for Fasciola hepatica Secreted Thioredoxin-1 and Peroxiredoxin-1. Frontiers in Cellular and Infection Microbiology, 11. doi:10.3389/fcimb.2021.667272DOI: 10.3389/fcimb.2021.667272
2020
Pritsch, I. C., Tikhonova, I. G., Jewhurst, H. L., Drysdale, O., Cwiklinski, K., Molento, M. B., . . . Verissimo, C. D. M. (2020). Regulation of the Fasciola hepatica newly excysted juvenile cathepsin L3 (FhCL3) by its propeptide: a proposed 'clamp-like' mechanism of binding and inhibition.. BMC molecular and cell biology, 21(1), 90. doi:10.1186/s12860-020-00335-5DOI: 10.1186/s12860-020-00335-5
Smith, D., Cwiklinski, K., Jewhurst, H., Tikhonova, I. G., & Dalton, J. P. (2020). An atypical and functionally diverse family of Kunitz-type cysteine/serine proteinase inhibitors secreted by the helminth parasite Fasciola hepatica.. Scientific reports, 10(1), 20657. doi:10.1038/s41598-020-77687-7DOI: 10.1038/s41598-020-77687-7
Murphy, A., Cwiklinski, K., Lalor, R., O'Connell, B., Robinson, M. W., Gerlach, J., . . . O'Neill, S. M. (2020). Fasciola hepatica Extracellular Vesicles isolated from excretory-secretory products using a gravity flow method modulate dendritic cell phenotype and activity.. PLoS neglected tropical diseases, 14(9), e0008626. doi:10.1371/journal.pntd.0008626DOI: 10.1371/journal.pntd.0008626
De Marco Verissimo, C., Jewhurst, H. L., Tikhonova, I. G., Urbanus, R. T., Maule, A. G., Dalton, J. P., & Cwiklinski, K. (2020). Fasciola hepatica serine protease inhibitor family (serpins): Purposely crafted for regulating host proteases.. PLoS neglected tropical diseases, 14(8), e0008510. doi:10.1371/journal.pntd.0008510DOI: 10.1371/journal.pntd.0008510
Shiels, J., Cwiklinski, K., Alvarado, R., Thivierge, K., Cotton, S., Gonzales Santana, B., . . . Dalton, J. P. (2020). Schistosoma mansoni immunomodulatory molecule Sm16/SPO-1/SmSLP is a member of the trematode-specific helminth defence molecules (HDMs).. PLoS neglected tropical diseases, 14(7), e0008470. doi:10.1371/journal.pntd.0008470DOI: 10.1371/journal.pntd.0008470
2019
Zhang, X. -X., Cwiklinski, K., Hu, R. -S., Zheng, W. -B., Sheng, Z. -A., Zhang, F. -K., . . . Zhu, X. -Q. (2019). Complex and dynamic transcriptional changes allow the helminth Fasciola gigantica to adjust to its intermediate snail and definitive mammalian hosts.. BMC genomics, 20(1), 729. doi:10.1186/s12864-019-6103-5DOI: 10.1186/s12864-019-6103-5
de la Torre-Escudero, E., Gerlach, J. Q., Bennett, A. P. S., Cwiklinski, K., Jewhurst, H. L., Huson, K. M., . . . Robinson, M. W. (2019). Surface molecules of extracellular vesicles secreted by the helminth pathogen Fasciola hepatica direct their internalisation by host cells.. PLoS neglected tropical diseases, 13(1), e0007087. doi:10.1371/journal.pntd.0007087DOI: 10.1371/journal.pntd.0007087
2018
McVeigh, P., Cwiklinski, K., Garcia-Campos, A., Mulcahy, G., O'Neill, S. M., Maule, A. G., & Dalton, J. P. (2018). In silico analyses of protein glycosylating genes in the helminth Fasciola hepatica (liver fluke) predict protein-linked glycan simplicity and reveal temporally-dynamic expression profiles.. Scientific reports, 8(1), 11700. doi:10.1038/s41598-018-29673-3DOI: 10.1038/s41598-018-29673-3
Dominguez, M. F., González-Miguel, J., Carmona, C., Dalton, J. P., Cwiklinski, K., Tort, J., & Siles-Lucas, M. (2018). Low allelic diversity in vaccine candidates genes from different locations sustain hope for Fasciola hepatica immunization.. Veterinary parasitology, 258, 46-52. doi:10.1016/j.vetpar.2018.06.011DOI: 10.1016/j.vetpar.2018.06.011
Hodgkinson, J. E., Cwiklinski, K., Beesley, N., Hartley, C., Allen, K., & Williams, D. (2018). Clonal amplification of Fasciola hepatica in Galba truncatula: within and between isolate variation of triclabendazole-susceptible and -resistant clones. Parasites and Vectors, 11, 9 pages. doi:10.1186/s13071-018-2952-zDOI: 10.1186/s13071-018-2952-z
Cwiklinski, K., & Dalton, J. P. (2018). Advances in Fasciola hepatica research using 'omics' technologies.. International journal for parasitology, 48(5), 321-331. doi:10.1016/j.ijpara.2017.12.001DOI: 10.1016/j.ijpara.2017.12.001
Cwiklinski, K., Jewhurst, H., McVeigh, P., Barbour, T., Maule, A. G., Tort, J., . . . Dalton, J. P. (2018). Infection by the Helminth Parasite <i>Fasciola hepatica</i> Requires Rapid Regulation of Metabolic, Virulence, and Invasive Factors to Adjust to Its Mammalian Host.. Molecular & cellular proteomics : MCP, 17(4), 792-809. doi:10.1074/mcp.ra117.000445DOI: 10.1074/mcp.ra117.000445
2017
Ruiz-Campillo, M. T., Molina Hernandez, V., Escamilla, A., Stevenson, M., Perez, J., Martinez-Moreno, A., . . . Cwiklinski, K. (2017). Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica.. Scientific reports, 7(1), 2782. doi:10.1038/s41598-017-03094-0DOI: 10.1038/s41598-017-03094-0
2016
Ravidà, A., Cwiklinski, K., Aldridge, A. M., Clarke, P., Thompson, R., Gerlach, J. Q., . . . O'Neill, S. M. (2016). Fasciola hepatica Surface Tegument: Glycoproteins at the Interface of Parasite and Host.. Molecular & cellular proteomics : MCP, 15(10), 3139-3153. doi:10.1074/mcp.m116.059774DOI: 10.1074/mcp.m116.059774
Smith, D., Tikhonova, I. G., Jewhurst, H. L., Drysdale, O. C., Dvořák, J., Robinson, M. W., . . . Dalton, J. P. (2016). Unexpected Activity of a Novel Kunitz-type Inhibitor: INHIBITION OF CYSTEINE PROTEASES BUT NOT SERINE PROTEASES.. The Journal of biological chemistry, 291(37), 19220-19234. doi:10.1074/jbc.m116.724344DOI: 10.1074/jbc.m116.724344
Cwiklinski, K., O'Neill, S. M., Donnelly, S., & Dalton, J. P. (2016). A prospective view of animal and human Fasciolosis.. Parasite immunology, 38(9), 558-568. doi:10.1111/pim.12343DOI: 10.1111/pim.12343
Garcia-Campos, A., Ravidà, A., Nguyen, D. L., Cwiklinski, K., Dalton, J. P., Hokke, C. H., . . . Mulcahy, G. (2016). Tegument Glycoproteins and Cathepsins of Newly Excysted Juvenile Fasciola hepatica Carry Mannosidic and Paucimannosidic N-glycans.. PLoS neglected tropical diseases, 10(5), e0004688. doi:10.1371/journal.pntd.0004688DOI: 10.1371/journal.pntd.0004688
2015
Cwiklinski, K., de la Torre-Escudero, E., Trelis, M., Bernal, D., Dufresne, P. J., Brennan, G. P., . . . Robinson, M. W. (2015). The Extracellular Vesicles of the Helminth Pathogen, <i>Fasciola hepatica</i>: Biogenesis Pathways and Cargo Molecules Involved in Parasite Pathogenesis. MOLECULAR & CELLULAR PROTEOMICS, 14(12), 3258-3273. doi:10.1074/mcp.M115.053934DOI: 10.1074/mcp.M115.053934
Beesley, N. J., Cwiklinski, K., Williams, D. J. L., & Hodgkinson, J. (2015). <i>Fasciola hepatica</i> from naturally infected sheep and cattle in Great Britain are diploid. PARASITOLOGY, 142(9), 1196-1201. doi:10.1017/S0031182015000499DOI: 10.1017/S0031182015000499
Cwiklinski, K., Allen, K., LaCourse, J., Williams, D., Paterson, S., & Hodgkinson, J. (2015). Characterisation of a novel panel of polymorphic microsatellite loci for the liver fluke, Fasciola hepatica, using a next generation sequencing approach. Infection, Genetics and Evolution, 32, 298-304. doi:10.1016/j.meegid.2015.03.014DOI: 10.1016/j.meegid.2015.03.014
Cwiklinski, K., Dalton, J. P., Dufresne, P. J., La Course, J., Williams, D., Hodgkinson, J., & Paterson, S. (2015). The Fasciola hepatica genome: gene duplication and polymorphism reveals adaptation to the host environment and the capacity for rapid evolution. Genome Biology, 16. doi:10.1186/s13059-015-0632-2DOI: 10.1186/s13059-015-0632-2
Fasciola hepatica vaccine: we may not be there yet but we're on the right road. (Journal article)
Molina-Hernández, V., Molina-Hernández, V., Mulcahy, G., Pérez, J., Martínez-Moreno, Á., Donnelly, S., . . . Cwiklinski, K. (2015). Fasciola hepatica vaccine: we may not be there yet but we're on the right road.. Veterinary parasitology, 208(1-2), 101-111. doi:10.1016/j.vetpar.2015.01.004DOI: 10.1016/j.vetpar.2015.01.004
2013
Identification of putative markers of triclabendazole resistance by a genome-wide analysis of genetically recombinant <i>Fasciola hepatica</i> (Journal article)
Hodgkinson, J., Cwiklinski, K., Beesley, N. J., Paterson, S., & Williams, D. J. L. (2013). Identification of putative markers of triclabendazole resistance by a genome-wide analysis of genetically recombinant <i>Fasciola hepatica</i>. PARASITOLOGY, 140(12), 1523-1533. doi:10.1017/S0031182013000528DOI: 10.1017/S0031182013000528
Transcriptome analysis of a parasitic clade V nematode: Comparative analysis of potential molecular anthelmintic targets in Cylicostephanus goldi (Journal article)
Cwiklinski, K., Merga, J. Y., Lake, S. L., Hartley, C., Matthews, J. B., Paterson, S., & Hodgkinson, J. E. (2013). Transcriptome analysis of a parasitic clade V nematode: Comparative analysis of potential molecular anthelmintic targets in Cylicostephanus goldi. International Journal for Parasitology, 43(11), 917-927. doi:10.1016/j.ijpara.2013.06.010DOI: 10.1016/j.ijpara.2013.06.010
2012
New insights into sequence variation in the IGS region of 21 cyathostomin species and the implication for molecular identification. (Journal article)
Cwiklinski, K., Kooyman, F. N. J., Van Doorn, D. C. K., Matthews, J. B., & Hodgkinson, J. E. (2012). New insights into sequence variation in the IGS region of 21 cyathostomin species and the implication for molecular identification.. Parasitology, 139(8), 1063-1073. doi:10.1017/s0031182012000467DOI: 10.1017/s0031182012000467
2009
Cloning and analysis of a Trichinella pseudospiralis muscle larva secreted serine protease gene. (Journal article)
Cwiklinski, K., Meskill, D., Robinson, M. W., Pozio, E., Appleton, J. A., & Connolly, B. (2009). Cloning and analysis of a Trichinella pseudospiralis muscle larva secreted serine protease gene.. Veterinary parasitology, 159(3-4), 268-271. doi:10.1016/j.vetpar.2008.10.036DOI: 10.1016/j.vetpar.2008.10.036
