HTI allows not only to quantitate the fluorescence intensity, but also to identify phenotypes and provide phenotypical and localisation data of heterogeneous cell populations. This approach gives the opportunity to screen multiple experimental conditions in an automatable and repeated fashion, both in fixed cells as end point assays as well as in live cell imaging.
Labelled cells were imaged in widefield mode in a 96 well plate. The cells were stained with a live (green) dead (magenta) labelling kit to follow the progression of cell health during the course of an infection. Data courtesy of Dan Foulkes (Fernig lab).
Microscopes
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