ProteoMMX 2.0

Duncan, chromatographer, mass spectrometrist, pescatorean, muso, ragtop-driver,

The STORY behind the man…

Duncan Robertson started working with Rob a long, long, time ago, in a laboratory far away (Lab 103, old Biochemistry department, Liverpool). He moved with Rob in 1993 to UMIST, taking on the role of Experimental Officer, and then came back to Liverpool in 2000, when Rob moved back to Liverpool.

He successfully navigated us across Brownlow Hill in the 'Big Move' and installed us in our new, wonderful labs.

Now he moves on to pastures new, swapping ITRAQ for IPAD, swapping protein models for financial models, swapping 'interesting problems' for 'interesting problems with attitude'.

We send him on his way, leaner, meaner, and ready for the world of professional services!

Duncan, there is a large group of individuals who owe their introduction to the world of protein mass spectrometry to you. They are your intellectual offspring, some grown up (some less so), but they all recognise and appreciate their debt to you.

Enjoy your new role, and remember, our door is always open!

Your friends in PFG

The leaving 'do'
Thanks to:

The Side Door (ace food!)
Lynn, for organising a great evening
Everyone, for coming along and having fun
Duncan (for agreeing to come and sort the BioLC - hehheh)

The EMBARASSING PHOTOS
Alongside...
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The CARD
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The RESEARCH.
(sung to the tune of the Beverly Hillbillies- if you don't know it, you missed out on the high point of the mid sixties! )

Urine, that is,
Behaviour Gold…
Semiochemical tea…

Now the next thing you know, 'ole Dunc's a busy bee
Purifying MUPs by chromatography
Attended a meeting about the role of MUPs in life
Came back with a collaborator and for Rob - a future wife!

His cologne: Calvin Urine OBSESSION

SmellPee

The PUBLICATIONS
1: Turton MJ, Robertson DH, Smith JR, Hurst JL, Beynon RJ. Roborovskin, a
lipocalin in the urine of the Roborovski hamster, Phodopus roborovskii. Chem
Senses. 2010 Oct;35(8):675-84. Epub 2010 Jun 24. PubMed PMID: 20576820.

2: Roberts SA, Simpson DM, Armstrong SD, Davidson AJ, Robertson DH, McLean L,
Beynon RJ, Hurst JL. Darcin: a male pheromone that stimulates female memory and
sexual attraction to an individual male's odour. BMC Biol. 2010 Jun 3;8:75.
PubMed PMID: 20525243; PubMed Central PMCID: PMC2890510.

3: Mudge JM, Armstrong SD, McLaren K, Beynon RJ, Hurst JL, Nicholson C, Robertson DH, Wilming LG, Harrow JL. Dynamic instability of the major urinary protein gene family revealed by genomic and phenotypic comparisons between C57 and 129 strain mice. Genome Biol. 2008;9(5):R91. Epub 2008 May 28. PubMed PMID: 18507838; PubMedCentral PMCID: PMC2441477.

4: Robertson DH, Wong SC, Beynon RJ, Hurst JL, Gaskell SJ. Observation of
heterogeneous gene products by FT-ICR MS. J Am Soc Mass Spectrom. 2008
Jan;19(1):103-10. PubMed PMID: 18293489.

5: McLean L, Young IS, Doherty MK, Robertson DH, Cossins AR, Gracey AY, Beynon
RJ, Whitfield PD. Global cooling: cold acclimation and the expression of soluble
proteins in carp skeletal muscle. Proteomics. 2007 Aug;7(15):2667-81. PubMed
PMID: 17623276.

6: Rivers J, Simpson DM, Robertson DH, Gaskell SJ, Beynon RJ. Absolute
multiplexed quantitative analysis of protein expression during muscle development
using QconCAT. Mol Cell Proteomics. 2007 Aug;6(8):1416-27. Epub 2007 May 17.
PubMed PMID: 17510050.

7: Robertson DH, Hurst JL, Searle JB, Gündüz I, Beynon RJ. Characterization and
comparison of major urinary proteins from the house mouse, Mus musculus
domesticus, and the aboriginal mouse, Mus macedonicus. J Chem Ecol. 2007
Mar;33(3):613-30. PubMed PMID: 17268823.

8: McDonald L, Robertson DH, Hurst JL, Beynon RJ. Positional proteomics:
selective recovery and analysis of N-terminal proteolytic peptides. Nat Methods.
2005 Dec;2(12):955-7. Epub 2005 Nov 18. PubMed PMID: 16299481.

9: Armstrong SD, Robertson DH, Cheetham SA, Hurst JL, Beynon RJ. Structural and
functional differences in isoforms of mouse major urinary proteins: a
male-specific protein that preferentially binds a male pheromone. Biochem J. 2005
Oct 15;391(Pt 2):343-50. PubMed PMID: 15934926; PubMed Central PMCID: PMC1276933.

10: Doherty MK, McLean L, Hayter JR, Pratt JM, Robertson DH, El-Shafei A, Gaskell
SJ, Beynon RJ. The proteome of chicken skeletal muscle: changes in soluble
protein expression during growth in a layer strain. Proteomics. 2004
Jul;4(7):2082-93. PubMed PMID: 15221769.

11: Simpson DM, Beynon RJ, Robertson DH, Loughran MJ, Haywood S.
Copper-associated liver disease: a proteomics study of copper challenge in a

12: Hayter JR, Robertson DH, Gaskell SJ, Beynon RJ. Proteome analysis of intact
proteins in complex mixtures. Mol Cell Proteomics. 2003 Feb;2(2):85-95. Epub 2003
Jan 21. PubMed PMID: 12644572.

14: Pratt JM, Petty J, Riba-Garcia I, Robertson DH, Gaskell SJ, Oliver SG, Beynon
RJ. Dynamics of protein turnover, a missing dimension in proteomics. Mol Cell
Proteomics. 2002 Aug;1(8):579-91. PubMed PMID: 12376573.

15: Beynon RJ, Veggerby C, Payne CE, Robertson DH, Gaskell SJ, Humphries RE,
Hurst JL. Polymorphism in major urinary proteins: molecular heterogeneity in a
wild mouse population. J Chem Ecol. 2002 Jul;28(7):1429-46. PubMed PMID:
12199505.

16: Pratt JM, Robertson DH, Gaskell SJ, Riba-Garcia I, Hubbard SJ, Sidhu K, Oliver SG, Butler P, Hayes A, Petty J, Beynon RJ. Stable isotope labelling in vivo as an aid to protein identification in peptide mass fingerprinting. Proteomics. 2002 Feb;2(2):157-63. PubMed PMID: 11840562; PubMed Central PMCID:PMC2705914.

17: Hurst JL, Payne CE, Nevison CM, Marie AD, Humphries RE, Robertson DH,
Cavaggioni A, Beynon RJ. Individual recognition in mice mediated by major urinary
proteins. Nature. 2001 Dec 6;414(6864):631-4. PubMed PMID: 11740558.

18: Darwish Marie A, Veggerby C, Robertson DH, Gaskell SJ, Hubbard SJ, Martinsen
L, Hurst JL, Beynon RJ. Effect of polymorphisms on ligand binding by mouse major
urinary proteins. Protein Sci. 2001 Feb;10(2):411-7. PubMed PMID: 11266626;
PubMed Central PMCID: PMC2373947.

19: Humphries RE, Robertson DH, Beynon RJ, Hurst JL. Unravelling the chemical
basis of competitive scent marking in house mice. Anim Behav. 1999
Dec;58(6):1177-1190. PubMed PMID: 10600138.

20: Wu C, Robertson DH, Hubbard SJ, Gaskell SJ, Beynon RJ. Proteolysis of native
proteins. Trapping of a reaction intermediate. J Biol Chem. 1999 Jan
8;274(2):1108-15. PubMed PMID: 9873058.

21: Hurst JL, Robertson DHL, Tolladay U, Beynon RJ. Proteins in urine scent marks
of male house mice extend the longevity of olfactory signals. Anim Behav. 1998
May;55(5):1289-97. PubMed PMID: 9632512.

22: Coughlan AR, Robertson DH, Bennett D, May C, Beynon RJ, Carter SD. Matrix
metalloproteinases 2 and 9 in canine rheumatoid arthritis. Vet Rec. 1998 Aug
22;143(8):219-23. PubMed PMID: 9770764.

23: Coughlan AR, Robertson DH, Burke R, Beynon RJ, Carter SD. Isolation and
identification of canine matrix metalloproteinase-2 (MMP-2). Vet J. 1998
May;155(3):231-7. PubMed PMID: 9638068.

24: Robertson DH, Hurst JL, Bolgar MS, Gaskell SJ, Beynon RJ. Molecular
heterogeneity of urinary proteins in wild house mouse populations. Rapid Commun
Mass Spectrom. 1997;11(7):786-90. PubMed PMID: 9161047.

25: Robertson DH, Cox KA, Gaskell SJ, Evershed RP, Beynon RJ. Molecular
heterogeneity in the Major Urinary Proteins of the house mouse Mus musculus.
Biochem J. 1996 May 15;316 ( Pt 1):265-72. PubMed PMID: 8645216; PubMed CentralPMCID: PMC1217333.

26: Beynon RJ, Oliver S, Robertson DH. Characterization of the soluble, secreted
form of urinary meprin. Biochem J. 1996 Apr 15;315 ( Pt 2):461-5. PubMed PMID:
8615815; PubMed Central PMCID: PMC1217218.

27: Lopes A, Daras V, Cross PA, Robertson G, Beynon G, Monaghan JM.
Thrombocytosis as a prognostic factor in women with cervical cancer. Cancer. 1994
Jul 1;74(1):90-2. PubMed PMID: 8004588.

28: Robertson G, Lopes A, Beynon G, Monaghan JM. Pelvic exenteration: a review of
the Gateshead experience 1974-1992. Br J Obstet Gynaecol. 1994 Jun;101(6):529-31. PubMed PMID: 8018644.

29: Beynon DW, Lopes A, Robertson G, Daras V, Monaghan JM. Squamous cell
carcinoma antigen: pretreatment levels as an indicator of advanced or metastatic
disease. Int J Gynecol Cancer. 1994 May;4(3):206-210. PubMed PMID: 11578408.

30: Evershed RP, Robertson DH, Beynon RJ, Green BN. Application of electrospray
ionization mass spectrometry with maximum-entropy analysis to allelic
'fingerprinting' of major urinary proteins. Rapid Commun Mass Spectrom. 1993
Oct;7(10):882-6. PubMed PMID: 8219320.

Centre for Proteome Research

Proteomics taking on questions in biology and medicine

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