CGR Metagenomics Workshop

9:00am - 5:00pm / Wednesday 15th May 2019 / Venue: Electrical Engineering & Electronics
Type: Workshop / Category: Research
  • Suitable for: Attendees are required to have basic linux command line skills. Candidates can be advised on how to gain these skills in an easy and free manner. For more information on the workshop and required prerequisites please contact Matthew Gemmell (matthew.gemmell@liverpool.ac.uk).
  • Admission: Registration fee: £300 (includes a workshop dinner) Application deadline: 5th April 2019 (please note that no more applications are being accepted).
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This 3-day (15th-17th May 2019) bioinformatics course is presented by the Centre for Genomic Research. It primarily focuses on practical computer sessions designed to provide delegates with hands-on experience of the informatics involved in analysing metagenomic and metagenetic datasets.
16S rRNA gene profiling (metagenetics) and shotgun sequence based metagenomics are now well established and can be utilised to characterise complex populations. The course will provide a 3-day introduction to the conceptual and practical issues involved in analysing such datasets, generated from the Illumina NGS platforms, and comprises the following sessions:
Sequence data QC and preparation for downstream analyses
Metagenomic shotgun sequence analysis.
Taxonomic profiling (Kraken & Bracken)
Metagenome assembly (MEGAHIT) and annotation (prodigal)
Functional annotation (EggNOG)
Data visualisation
Analysis of 16S rRNA data with QIIME2
Laboratory tour of sequencing laboratory
Metagenome functional prediction using metagenetic data (PICRUSt)