GSTT SEMINAR: Dr Sean Connell, CIC bioGUNE - Title: 'Disassembling Ribosome Assembly'
1:00pm - 2:00pm /
Monday 5th December 2016
/ Venue: Lecture Theatre 1 Life Sciences Building
- Mark Caddick
- Admission: Free
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Abstract:
The bacterial (70S) ribosome consists of 3 RNA molecules and dozens of ribosomal proteins (r-proteins) which assemble in a complex and highly coordinated process. In vivo the assembly process is facilitated by several trans-acting factors called ribosome assembly factors. These factors can serve as checkpoints to monitor the assembly process and/or actively promote rRNA-folding and r-protein/rRNA interactions to shape the folding landscape. Here we present a cryo-EM structure of the ribosome assembly factor, RsgA, bound to the bacterial 30S subunit. This structure reveals potential late stage conformations of the 30S subunit and mechanisms used by RsgA to guide 30S maturation.
The bacterial (70S) ribosome consists of 3 RNA molecules and dozens of ribosomal proteins (r-proteins) which assemble in a complex and highly coordinated process. In vivo the assembly process is facilitated by several trans-acting factors called ribosome assembly factors. These factors can serve as checkpoints to monitor the assembly process and/or actively promote rRNA-folding and r-protein/rRNA interactions to shape the folding landscape. Here we present a cryo-EM structure of the ribosome assembly factor, RsgA, bound to the bacterial 30S subunit. This structure reveals potential late stage conformations of the 30S subunit and mechanisms used by RsgA to guide 30S maturation.