What can we learn from Genomics and Transoposon Mutagenesis in the (Fission) Yeast Model?

4:00pm - 5:00pm / Tuesday 26th February 2019 / Venue: Lecture Theatre 1 Life Sciences Building
Type: Seminar / Category: Research / Series: BEEM Seminar
  • Suitable for: Staff and students with an interest in Behaviour, Evolution, Ecology and Microbiology
  • Admission: Free
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Speaker: Daniel Jeffares (University of York)

The fission yeast Schizosaccharomyces pombe is a powerful model for molecular and cellular biology. In contrast to the budding yeast, we knew very little about the diversity, ecology or evolution of the species until very recently.

I’ll introduce what little is known about the ecology of this yeast (1), and then describe three of my studies of genome diversity and function. First, I’ll outline our study of genomic and phenotypic diversity, where we describe population structure, date the dispersal of the species and show that genome-wide association studies are feasible in this species (2). I’ll then describe our analysis of structural variation from short read and long read sequencing data (3, 4) demonstrating that copy number variants both contribute to heritable traits, are unstable, and that quantitative traits are explained by an ancient admixture event from two populations.

On a slightly different track, I’ll describe our recent analysis of saturating transposon mutagenesis in S. pombe (5). In this study, we generated very dense transposon insertion libraries using the Hermes transposon, to one insertion per 13 nt of the genome. We developed a hidden Markov Model that uses the transposon insertion density to classify the relative importance of each position in the genome. We show that HMM states assign similar functional constraints to comparative genomics and genetic diversity, but with far higher resolution. This data will bring us closer to quantifying the functional significance of every base in the genome.

References
1. D. C. Jeffares, The natural diversity and ecology of fission yeast. Yeast. 35, 253–260 (2017).
2. D. C. Jeffares et al., The genomic and phenotypic diversity of Schizosaccharomyces pombe. Nature Genetics. 47, 235–241 (2015).
3. D. C. Jeffares et al., Transient structural variations have strong effects on quantitative traits and reproductive isolation in fission yeast. Nat Commun. 8, 14061 (2017).
4. S. Tusso et al., Ancestral admixture and structural mutation define global biodiversity in fission yeast. bioRxiv, 415091 (2018).
5. L. Grech et al., Fitness Landscape of the Fission Yeast Genome. bioRxiv, 398024 (2018).