Chemistry Seminar - The Design of Miniature Bio-assay Devices - David E Williams
11:00am - 12:00pm /
Friday 18th September 2015
Type: Conference /
Category: Department
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There is a strong drive to develop home-use diagnostic devices, for example for management of heart failure patients. These would need to work on sample volumes as low as 1uL (a small finger prick) and represent a very significant measurement challenge (1pM in 1uL is only 600000 molecules). The current technology drive is to use capillary-fill microfluidic systems and high-brightness nanoparticle labels. Precision and accuracy of measurement is important for these devices since important treatment decisions may be made on the results. The device and label surfaces, the reagents, structures for mixing, timing and capture, and the signal transduction all need to be designed carefully with precision in mind. The preparation and fabrication procedures need to be reliable and scalable for high-volume manufacture. Reliable modelling of the connection between the signal generation and the concentration of the analyte is also needed to show what device design parameters most significantly impact on the errors. This talk discusses:
1. Design parameters – the unit operations, the system specification and how these interact
2. A new industrially-scalable method for manufacturing active surfaces on biosensors using plasma-enhanced chemical vapour deposition, and methods for evaluating such surfaces
3. Antibodies on surfaces – the conformation, binding capacity, non-specific binding and how the choice of surface influences these
4. Model development leading to understanding of precision drivers for the case where the signal generating label is a nanoparticle and specifically treating lateral flow devices such as the home pregnancy test.
The results concerning the key error drivers in the device design can be surprising and indicate some interesting directions for future research.
1. Design parameters – the unit operations, the system specification and how these interact
2. A new industrially-scalable method for manufacturing active surfaces on biosensors using plasma-enhanced chemical vapour deposition, and methods for evaluating such surfaces
3. Antibodies on surfaces – the conformation, binding capacity, non-specific binding and how the choice of surface influences these
4. Model development leading to understanding of precision drivers for the case where the signal generating label is a nanoparticle and specifically treating lateral flow devices such as the home pregnancy test.
The results concerning the key error drivers in the device design can be surprising and indicate some interesting directions for future research.