Module Specification |
The information contained in this module specification was correct at the time of publication but may be subject to change, either during the session because of unforeseen circumstances, or following review of the module at the end of the session. Queries about the module should be directed to the member of staff with responsibility for the module. |
Title | Protein Structure, Function and Organisation | ||
Code | LIFE303 | ||
Coordinator |
Prof L Lian Biochemistry Lu-Yun.Lian@liverpool.ac.uk |
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Year | CATS Level | Semester | CATS Value |
Session 2016-17 | Level 6 FHEQ | First Semester | 15 |
Pre-requisites before taking this module (other modules and/or general educational/academic requirements): |
LIFE203 None |
Modules for which this module is a pre-requisite: |
Co-requisite modules: |
Linked Modules: |
Teaching Schedule |
Lectures | Seminars | Tutorials | Lab Practicals | Fieldwork Placement | Other | TOTAL | |
Study Hours |
22 |
1 |
23 | ||||
Timetable (if known) |
This refers to timetabled lectures
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This refers to a timetabled bioinformatics workshop
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Private Study | 127 | ||||||
TOTAL HOURS | 150 |
Assessment |
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EXAM | Duration | Timing (Semester) |
% of final mark |
Resit/resubmission opportunity |
Penalty for late submission |
Notes |
Unseen Written Exam | 1.5 hrs | Semester 1 | 50 | Yes | Standard UoL penalty applies | Assessment 1 |
Unseen Written Exam | 2 hrs | Semester 1 | 50 | Yes | Standard UoL penalty applies | Assessment 2 Notes (applying to all assessments) Assessment 1 will be a written examination. Assessment 2 will be 2 written assignments of equal weighting, written under examination conditions. |
CONTINUOUS | Duration | Timing (Semester) |
% of final mark |
Resit/resubmission opportunity |
Penalty for late submission |
Notes |
Aims |
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Learning Outcomes |
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To describe in detail, current methods used for the determination and analysis of protein structures |
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To critically discuss how proteomics-based approaches can be used to study fundamental and applied biological problems |
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To discuss the latest methods of analysis of post-translational modifications of proteins and implications for cell function |
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To critically evaluate the strengths and weakness of the different technologies - nuclear magnetic resonance (NMR), x-ray crystallography- mass spectrometry and how they can be used in an integrated manner |
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To discuss the prerequisites for obtaining structures such as protein foldedness and sample preparations |
Teaching and Learning Strategies |
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Lecture - This refers to timetabled lectures |
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Workshop - This refers to a timetabled bioinformatics workshop |
Syllabus |
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1 |
Methods of proteome analysis: Revision of protein structure. Primary structure and amino acid modifications. Identification of proteins from
primary structure: Use of molecular mass of proteins and peptides to determine amino acid composition and sequence.
Basic proteomic methods: Basics of mass spectrometry.
Applications of proteomic techniques: Use in human and microbes.
Applications of proteomic techniques: Subcellular proteomics. Post-translational modifications.
Bringing together the techniques of structural biology: Overview of techniques and applications
Recombinant Protein Production: Methods for making recombinant proteins and purification
Nuclear magnetic resonance: Structural determination. Molecula
r dynamics & interactions
X-ray crystallography: X-ray crystallography. Applications
Advanced methods: Synchrotron-based structural methods for structure determination of proteins.
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Recommended Texts |
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Reading lists are managed at readinglists.liverpool.ac.uk. Click here to access the reading lists for this module. Explanation of Reading List: Students will be directed to current primary research articles. |