Module Specification
The information contained in this module specification was correct at the time of publication but may be subject to change, either during the session because of unforeseen circumstances, or following review of the module at the end of the session. Queries about the module should be directed to the member of staff with responsibility for the module.
Title Advanced Biochemical Techniques
Code LIFE224
Coordinator Dr AD Bates
Biochemistry
A.D.Bates@liverpool.ac.uk
Year CATS Level Semester CATS Value
Session 2016-17 Level 5 FHEQ Second Semester 7.5

Pre-requisites before taking this module (other modules and/or general educational/academic requirements):
LIFE122 None 

Modules for which this module is a pre-requisite:
 

Co-requisite modules:
 

Linked Modules:
 

Teaching Schedule
  Lectures Seminars Tutorials Lab Practicals Fieldwork Placement Other TOTAL
Study Hours 5

     28

   7

4

 44
Timetable (if known) is refers to timetabled lectures at the start of the practical sessions
  		    This refers to practical sessions in laboratories, including groupwork
  		  This refers to timetabled group writing sessions at the end of the two practical projects.
This refers to in course, summative assessment.
  		 
Private Study 31
TOTAL HOURS 75

Assessment
EXAM Duration Timing
(Semester)		 % of
final
mark		 Resit/resubmission
opportunity		 Penalty for late
submission		 Notes
             
CONTINUOUS Duration Timing
(Semester)		 % of
final
mark		 Resit/resubmission
opportunity		 Penalty for late
submission		 Notes
Practical Assessment  3 hours  Semester 2  50  No reassessment opportunity  Standard UoL penalty applies  Assessment 1 There is no reassessment opportunity,  
Coursework  1 hour  Semester 2  20  Yes  Standard UoL penalty applies  Assessment 2 
Coursework  2x2500 words  Semester 2  30  No reassessment opportunity  Standard UoL penalty applies  Assessment 3 There is no reassessment opportunity, Notes (applying to all assessments) Assessment 224 will be a practical and data handling skills exam. Assessment 224.1 will be based on completing the coursework assignments within the laboratory classes. Assessment 224.2 will be the generation of two Scientific Reports (one on each practical project), written as a group work exercise. 

Aims

  • This module aims to:

     
    Provide students with a practical training in a number of techniques used in biochemistry, including analysis of enzyme activity and stability and protein purification and analysis using chromatography and electrophoresis;

  • Develop in students the knowledge, understanding and ability to design experiments, and to apply, evaluate and interpret experimental data to solve problems in biochemistry and molecular cell biology.


    • Learning Outcomes

    On successful completion of this module students will be able to:

     
    Present, critically evaluate and interpret qualitative and quantitative data, and record procedures and protocols;

    Develop team-working and individual skills, learn to manage time effectively and use laboratory equipment correctly and safely to generate data;
    Plan and execute a series of biochemical experiments to analyse protein structure and function;

    Analyse data, interpret its validity and apply statistical analyses.


    Teaching and Learning Strategies

    Lecture -

    is refers to timetabled lectures at the start of the practical sessions

    Laboratory Work -

    This refers to practical sessions in laboratories, including groupwork

    Workshop -

    This refers to timetabled group writing sessions at the end of the two practical projects.

    Assessment -

    This refers to in course, summative assessment.

    Syllabus
    1 

    Two extended practical projects will be conducted using a ''problem solving'' format. Emphasis will be placed upon the rational interpretation of experimental data obtained by the students.  Students will work in teams to plan programmes of experiments, to carry out the experiments, to draw conclusions from pooled data and to write up final reports.

     
    Group 1                 
    Sessions 1-3< /font>
    Protein separation by ion exchange and gel filtration chromatography. Protein assay and analysis by polyacrylamide gel electrophoresis and Western blotting
     
    Sessions 4-5
    Analysis and use of an immobilised enzyme - assays of enzyme activity and stability.
     
    Session 6
    Practical and data handling assessments
     
    Group 2
    Sessions 1-2
    Analysis and use of an immobilised enzyme - assays of enzyme activity and stability.
     
    Sessions 3-5
    Protein separation by ion exchange and gel filtration chromatography. Protein assay and analysis by polyacrylamide gel electrophoresis and Western blotting
     
    Session 6
    Practical and data handling assessments

    Recommended Texts
    Reading lists are managed at readinglists.liverpool.ac.uk. Click here to access the reading lists for this module.
    Explanation of Reading List: