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Media to demonstrate development of tissue culture in the African Violet

MS salts g.l-1

NAA mg.l-1

BAP mg.l-1

sucrose %

1. Control

4.71

0.5

0.5

3.0

2. vary sucrose - 0 as control as control as control

0.0

3. vary sucrose -1% as control as control as control

1.0

4. vary sucrose - 5% as control as control as control

5.0

5. vary BAP - 0 as control as control

0.0

as control
6. vary BAP - 1/5th as control as control

0.1

as control
7. vary BAP - x10 as control as control

5.0

as control
8. vary NAA - 0 as control

0

as control as control
9. vary NAA - 1/5th as control

0.1

as control as control
10. vary NAA - x10 as control

5.0

as control as control
11. vary MS  - 0

0

as control as control as control
12. vary MS - 1/10th

0.47

as control as control as control
13. vary MS - x2

9.52

as control as control as control

Key to abbreviations:

MS salts - Murashige and Skoog inorganic medium

NAA - naphthalene acetic acid (auxin)

BAP - benzylamine purine (cytokinin)

Murashige and Skoog (MS) is one of the most commonly used basic media in plant tissue culture work. It is regarded as a 'high salt' medium because of its macroelement composition.

Since accurate and reproducible preparation of the growth medium is essential, it is now usually obtained from a commercial source in powdered or liquid concentrate. Growth regulators are purchased similarly.

All media are solidified with 10 g.l-1 agar, and sterilised by autoclaving, then dispensed into 9cm petri dishes

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