Photo of Em P Rob Beynon

Em P Rob Beynon B.Sc (Hons), Ph.D. FLSW

Professor Biochemistry & Systems Biology

    Research

    Research Interests

    His main areas of research are in the areas of protein chemistry, proteomics, proteolysis, and proteolytic. In particular, his research programmes are dedicated to the application of modern methods of protein analysis to problems in biology. He has pioneered the areas of understanding protein dynamics on a global, proteome wide scale and of developing relevant methods in intact animals and complex systems. He has pioneered the areas of understanding protein dynamics on a global, proteome wide scale and of developing relevant methods in intact animals and complex systems. With colleagues, he developed a wholly new strategy for multiplexed absolute quantification in proteomics (QconCAT) that has been applied to diverse areas such as muscle development and expression of polymorphic proteins in chemical communication. He has described a new strategy for rational proteome simplification that has created new opportunities for rapid proteome profiling. His website for the calculation of thermodynamically correct buffers (www.liv.ac.uk/buffers) has helped the scientific community to prepare over three quarters of a million litres of buffers!

    Research Grants

    ALACATS: bespoke strategy for absolute protein quantification

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    December 2019 - November 2021

    Unlocking the molecular stoichiometry of functional CO2-fixing organelles for synthetic engineering

    ROYAL SOCIETY (OST)

    October 2018 - March 2022

    The effect of Gastro-oesophageal reflux on CF lung disease

    VERTEX PHARMACEUTICALS INC (USA)

    June 2017 - August 2021

    KTP with Badrilla Ltd

    BADRILLA LTD (UK), INNOVATE UK (UK)

    February 2018 - January 2020

    Use of REIMS in ecological analysis

    MICROMASS UK LIMITED (UK)

    October 2017 - March 2022

    Double standards in quantitative proteomics: Development of calibrators for multiplexed quantitative western blotting or mass spectrometry

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    May 2015 - April 2016

    Role of serine protease activity in the pathogenesis of muscular dystrophy

    MUSCULAR DYSTROPHY ASSOCIATION USA)

    September 2015 - January 2019

    Understanding and exploiting the urinary proteins of New Zealand's leading mammal pest

    ROYAL SOCIETY OF NEW ZEALAND (NEW ZEALAND)

    February 2017 - October 2019

    analysis of MUPs in Mus species and hybrids”

    FRENCH NATIONAL CENTRE FOR SCIENTIFIC RESEARCH (CNRS) (FR)

    September 2015 - July 2020

    Bayesian Quantitative Proteomics

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    September 2015 - March 2019

    Multiplexed Efficient Expression of Recombinant Qconcats

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    June 2017 - July 2017

    Unilever Framework Grant

    UNILEVER UK CENTRAL RESOURCES LTD (UK)

    July 2015 - July 2019

    Development of a quantitative assay for pepsin in bronchoalveolar lavage (BAL) as a tool for clinical diagnoses

    BIOCHEMICAL SOCIETY (UK)

    July 2016 - August 2016

    Sparking Impact

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    June 2013 - July 2014

    YEAR 1 Wellcome Trust ISSF Non-Clinical Fellowships

    WELLCOME TRUST (UK)

    October 2011 - May 2018

    A new form of scent communication in house mice: navigation cues.

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    October 2001 - April 2005

    Identification of vaccine candidates in larval excretory/secretory products of Teladorsagia circumcincta.

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    January 2007 - December 2009

    BIOMAH - Novel strategies for biomarker discovery and quantification

    EUROPEAN COMMISSION

    April 2009 - May 2011

    SILAC analysis of the HGF signalling.

    CANCER RESEARCH UK (CRUK) (UK)

    October 2005 - September 2008

    FPGA supercomputing technology for high-throughput identification and quantitation in proteomics

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    March 2008 - March 2011

    Rapid proteome profiling using positional signature peptides

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    June 2008 - May 2010

    Northwest consortium for third generation proteomics.

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    November 2001 - November 2004

    The interplay of rodent behaviour, semiochemistry and systems: from scientific principles to control strategy

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    July 2012 - July 2018

    Proposal to Establish a Bioinformatics Laboratory.

    WOLFSON FOUNDATION (UK)

    January 2001 - July 2001

    Applying innovative technologies to improve the benefit-risk ratio of drugs: developing a national resource underpinned by the infrastructure of the MRC Centre for Drug Safety Science

    MEDICAL RESEARCH COUNCIL (MRC)

    April 2015 - March 2016

    MUP knockout mice: implications for chemical communication and a generic research tool.

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    May 2006 - July 2010

    Double standards in proteomics

    BADRILLA LTD (UK), BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    March 2012 - July 2012

    The Liverpool Bespoke Oligoarray Facility (L-BOF).

    NATURAL ENVIRONMENT RESEARCH COUNCIL (NERC)

    January 2005 - January 2008

    Proteomics analysis of keratinocyte. A Preliminary Investigation

    UNILEVER PLC (UK)

    October 2013 - November 2013

    Partnership in applied proteomic technologies: understanding the academic/industrial perspectives.

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    June 2010 - November 2012

    Linking the proteome and transcriptome - assessment of the relationship between proteome ubiquitination and turnover.

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    October 2004 - September 2007

    MUPs, MHC and mate choice in house mice.

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    May 2004 - April 2008

    Myofibroblast control of oesophageal squamous cancer invasion: role of chemerin

    NORTH WEST CANCER RESEARCH FUND, NORTH WEST CANCER RESEARCH INCORPORATING CLATTERBRIDGE CANCER RESEARCH (UK)

    February 2015 - December 2018

    Characterisation of two antigen complexes for use in a diagnostic assay for larval stage cyathostomins.

    THE HORSE TRUST (UK)

    March 2003 - February 2004

    Application of advanced MS instrumentation to protein ligand binding and PTM characterisation

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    January 2015 - January 2016

    Dynamics and function of the NF-kappaB signalling system

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    May 2008 - July 2014

    Ecoyeast - Mastering the economics of adaptation through constraint-based modeling in yeast

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    March 2015 - June 2018

    Double Standards in Proteomics

    BADRILLA LTD (UK)

    October 2012 - September 2016

    The decision to cooperate: kin recognition mechanisms in female house mice

    NATURAL ENVIRONMENT RESEARCH COUNCIL (NERC)

    March 2010 - March 2014

    Exploiting Genomic Information: Strategies for Cross-Species Identification in Proteomics.

    NATURAL ENVIRONMENT RESEARCH COUNCIL (NERC)

    January 2002 - September 2005

    Bio-Chemical and Behavioural Mechanisms of Olefactory Communication in Wild House Mice.

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    February 2000 - March 2001

    Investigating cartilage matrix turnover in health and disease using a quantitative proteomics approach

    WELLCOME TRUST (UK)

    September 2009 - September 2015

    Identification of candidate protective antigens for vaccination against the bovine lungworm, Dictyocaulus viviparus.

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    March 2003 - March 2006

    A proteomics approach to quantify adaptive variation in mammalian ejaculates

    NATURAL ENVIRONMENT RESEARCH COUNCIL (NERC)

    January 2012 - December 2014

    Molecular changes that characterise the unique response of North Ronaldsay sheep to copper challenge compared with domesticated (Cambridge) sheep.

    WELLCOME TRUST (UK)

    July 2001 - January 2004

    Automatic monitoring and control of small mammals in large and complex spaces.

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    April 2003 - April 2006

    The PPP-labels software for quantitative proteomics

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    September 2014 - September 2015

    Mass spectrometry imaging for biology and biotechnology

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    January 2014 - December 2014

    Keeping up with the bases: 'nextgen' approaches to proteomics

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    July 2013 - July 2014

    Turnover profiling: commitment and degradation of the yeast proteome.

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    January 2001 - December 2004

    A systems biology study of E2F and NF-kappaB cross-talk

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    February 2010 - April 2014

    Hair proteomics and metabolomics for biomarker profiling

    WELLCOME TRUST (UK)

    July 2006 - August 2006

    A convergent strategy for high efficiency quantitative proteomics.

    ENGINEERING & PHYSICAL SCIENCES RESEARCH COUNCIL (EPSRC)

    October 2005 - September 2008

    Application of field programmable gate arrays to eliminate bottlenecks in near-instrument proteomics.

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    November 2004 - November 2007

    Development of an assay to detect subclinical ragwort poisoning in horses.

    THE HORSE TRUST (UK)

    January 2004 - December 2007

    Between animal and between farm transmission: the role of behaviour, signalling, host genetics and management (Pathogen transmission).

    DEPARTMENT FOR ENVIRONMENT, FOOD AND RURAL AFFAIRS (UK)

    April 2004 - December 2009

    Global quantification of the yeast proteome

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    August 2009 - December 2014

    Definition of the Skeletal Muscle 'Degradome' - Metabolic Turnover of the Proteome.

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    June 2002 - January 2005

    Analysis of peptides and proteins using liquid-chromatography linked to electrospray ionisation time of flight mass spectrometry.

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    January 2001 - January 2004

    The communication of complex information in rodent scent marks through proteins and low molecular weight components.

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    January 2001 - April 2004

    Ratting on rats - who, what, where and when?

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    June 2004 - May 2005

    Application of quantitative proteomics to accelerate vaccine development against Schistosomiasis, a neglected tropical disease

    DEPARTMENT FOR BUSINESS, INNOVATION AND SKILLS (UK)

    January 2015 - December 2015

    Characterization of the endothelial cell surface molecule(s) responsible for galectin-3-induced secretion of metastasis-promoting cytokines

    NORTH WEST CANCER RESEARCH FUND, NORTH WEST CANCER RESEARCH INCORPORATING CLATTERBRIDGE CANCER RESEARCH (UK)

    July 2014 - March 2017

    Chromatography-free proteomics: a 'nextgen' perspective

    ROYAL SOCIETY (CHARITABLE)

    January 2012 - December 2014

    Composition, heterogeneity and dynamics of the eukaryotic translation machinery

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    October 2008 - December 2011

    Extending absolute quantification to the proteome-wide scale.

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL (BBSRC)

    March 2005 - September 2008

    New approaches to quantitative proteomics: application to chemical communication

    WATERS CORPORATION (UK)

    October 2011 - September 2015

    A new approach to proteomics using binders and top-down mass spectrometry

    AVACTA GROUP PLC (UK)

    October 2013 - September 2017

    Research Collaborations

    Prof J McCarthy

    External: The University of Manchester

    Collaboration on proteomics of eukaryotic translatosome

    Dr Philip Whitfield

    Internal

    Prof Jane Hurst

    Internal

    Dr SImon Hubbard

    External: The University of Manchester

    Prof Simon Gaskell

    External: The University of Manchester