Photo of Prof Mandy Peffers

Prof Mandy Peffers BSc(Hons)MPhil PhD BVetMed MRCVS

Wellcome Trust Clinical Intermediate Fellow Musculoskeletal Biology I

    Research

    Defining the role and mechanisms of small nucleolar RNAs in cartilage ageing and disease

    This project aims to test the hypothesis that the age-related dysregulation of the expression of a defined set of small nucleolar RNAs (snoRNAs) alters the chondrocyte phenotype through altered translational capacity which contributes to the development of osteoarthritis. We will determine what snoRNAs are altered in ageing and/or osteoarthritis, what their role is in cartilage ageing and OA and how they alter the risk of OA. Altering the expression of these snoRNAs will change disease progression and enable targets for therapeutics to be identified.

    Stratification of osteoarthritis to improve equine welfare

    I have a Horse Trust funded studentship being undertaken by James Anderson. This studentship aims to identify proteomic biomarkers and metabolomics signatures in articular tissues, synovial fluids and serum which can be used to stratify horses with osteoarthritis into disease subtypes. Specific proteomic and metabolomics variables, which distinguish osteoarthritis subtypes, will be developed into clinically applicable assays and initial longitudinal clinical studies will be performed to identify the potential role of OA stratification in predicting treatment response.

    Tendon proteomics, epigenetics and transcriptomics in ageing and disease

    I have undertaken a number of studies (using next generation sequencing technologies such as RNASeq, microarray, miRNASEq, mass spectrometry-based proteomics) on horse, rat and human tendon in order to identify the molecular mechanisms underlying tendon ageing and disease.

    Currently I have a PhD student funded by CIMA investigating the epiegenetics of tendon ageing through the interrogation of DNA methylation and small non-coding RNAs.

    Research Grants

    Investigating the role and mechanisms of small non-coding RNAs in chondrosarcoma: A small RNA sequencing approach.

    SARCOMA UK (UK)

    September 2018 - February 2021

    Small non-coding RNAs to stratify osteoarthritis in the horse

    THE HORSE TRUST (UK)

    June 2019 - May 2022

    Bench fees - Mr Aibek Smagul

    MINISTRY OF EDUCATION AND SCIENCE GOVERNMENT OF THE REPUBLIC OF KAZAKHSTAN (KAZAKHSTAN)

    September 2016 - August 2020

    In-vitro study of the effects of magnesium, morphine and mepivavaine on equine joints

    HORSERACE BETTING LEVY BOARD (UK)

    February 2015 - December 2016

    2014 allocation - Wellcome ISSF non clinical fellowships

    WELLCOME TRUST (UK)

    May 2015 - October 2018

    Defining the role and mechanisms of small nucleolar RNAs in cartilage

    WELLCOME TRUST (UK)

    September 2015 - March 2021

    The stratification of osteoarthritis to improve equine welfare

    THE HORSE TRUST (UK)

    September 2015 - August 2018

    Determining novel mechanisms of joint destruction as biomarker for arthropathy

    LIVERPOOL HEALTH PARTNERS (UK)

    June 2013 - July 2016