For measurement of trabecular architecture and osteoid, sections were stained with van Gieson/von Kossa, although Masson’s trichrome has also been successfully used. The program starts with identification of the area to be analysed and identification of the bone by thresholding as described for TrapHisto. The next step identifies the osteoid by thresholding:


The results are again presented as an Excel-compatible .csv file: