MTMR3 subcloned constructs (see maxiprep stocks link):

Note: Stop mutation in 197 position of MTMR3 was wrong so this fragment is the same than its original KTYP and will correspond to 1-283 fragment in pGEXT vectors

- pAcHis-Kiaa0371(MTMR3; for virus)

- pAcHis-KC1174S (FYVE mutant; for virus)

- pAcHis-KD316S (Trap mutant; for virus)

- pAcG2T-K2 (MTMR3 frame 2; for virus)

- pAcG2T-K2-D316S (Trap mutant; for virus)

- pAcG2T-ΔPHG (Deletion of its PHG domain; for virus)

- pUG36-K-C1174S (FYVE mutant; for yeast)

- pGEM-T-KPHG1 (2nd fragment of the tandem PHG)

- pGEM-T-KPHG3 (1st fragment of the tandem PHG)

- pGEM-T-K(1-193) (MTMR3 fragment 1 to 193 aa)

- pGEM-T-KTyp-S56A/R59C (MTMR3 fragment 1 to 490 with conserved MTM1 disease-linked mutations)

- pGEM-T-KTyp-K92/K95A (MTMR3 fragment 1 to 490 with conserved MTM1 disease-linked mutations)

- pGEM-T-KTyp-R59C (MTMR3 fragment 1 to 490 with conserved MTM1 disease-linked mutation)

- pGEM-T-KTyp (MTMR3 fragment 1-490)

- pGEM-T-K2-ΔPHG-D316A (deletion of PHG domain with Trap mutation)

- pGEM-T-K2-ΔPHG (deletion of PHG domain)

- pGEM-T-K2-D316A (Trap mutation)

- pGEM-T-K2-C1174S (FYVE domain mutation in frame 2)

- pGEM-T-KC413S (Phosphatase domain mutation)

- pGEM-T-KC1174S (FYVE domain mutation in frame 1)

- pGEM-T-K2 (MTMR3 frame 2)

- pGEM-T-Kiaa0371 (MTMR3 frame1)

- pGEM-T-K2-S56A/R59C (MTMR3 with conserved MTM1 disease-linked mutations)

- pGEM-T-K2-K92/K95A (MTMR3 with conserved MTM1 disease-linked mutations)

- pGEM-T-K2-R59C (MTMR3 with conserved MTM1 disease-linked mutation)

- pFBT9-MTMR3 (Y2H, MTMR3 in the bait) (GBT9 map, same but Amp resistence)

- pFBT9-C413S (Y2H, Phosphatase mutant MTMR3 in the bait) (no map)

- pACT2-MTMR3 (Y2H, MTMR3 in the prey)

- pHA-ΔPHG/C413/1174S (deletion of PHG domain + phosphatase mutation + FYVE mutation)

- pHA-ΔPHG/C413S (deletion of PHG domain + phosphatase mutation)

- pHA-ΔPHG/D316A (deletion of PHG domain + Trap mutation)

- pHA-ΔPHG (deletion of PHG domain of MTMR3)

- pHA-K2D316A (Trap mutation)

- pHA-C413/1174S (phosphatase/FYVE-domain mutant)

- pHA-C413S (phosphatase mutant)

- pHA-C1174S (FYVE-domain mutant)

- pHA-Kiaa0371 (MTMR3 HA-tagged vector)

- pEGFP-C2-MTMR3 (MTMR3 tagged vector)

- pEGFP-C2-C413S (Phosphatase mutant MTMR3)

- pEGFP-C2-K(1-821) (deletion of FYVE and coiled-coil domains)

- pEGFP-C1-2xPHG (tanden isolated MTMR3-PHG domain)

-pRFP-C1-2xPHG (tanden isolated MTMR3-PHG domain in red-tagged vector)

- pGEXT-4T2-K-2xPHG (tandem 2xPHG domain of MTMR3)

- pGEXT-4T2-K-PHG (1xPHG domain)

- pGEXT-4T2-K(1-193) (fragment 1 to 193 aa)

- pGEXT-4T2-K(1-283)-K92/K95A (fragment 1 to 283 with conserved MTM1 disease-linked mutations)

- pGEXT-4T2-K(1-283)-S56A/R59C (fragment 1 to 283 with conserved MTM1 disease-linked mutations)

- pGEXT-4T2-K(1-283)-R59C (fragment 1 to 283 with conserved MTM1 disease-linked mutation)

- pGEXT-4T2-K(1-283) (fragment 1 to 283 of MTMR3 in Gext tagged vector)

-pTrc-HisA-KTYP (MTMR3 fragment 1-490 in His tagged vector)