BIOL301 - DNA Replication and the Cell Cycle

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BIOL301 - DNA Replication and the Cell Cycle

Module co-ordinator : Dr. A.G. McLennan

Location

Life Sciences LT1

Times

Wednesday: 09.00, Friday: 10.00

Week
Lecture Content

1-3
5 Dr S. Pennington
Introduction to the eukaryotic cell cycle

Introduction. Major events and checkpoints of the cell cycle.

Identification of the cell cycle machinery - the cyclins and cyclin-dependent kinases.

Regulation of cell cycle checkpoints by cyclin-dependent kinases.

Growth factor regulation of cell cycle progression.

Cell shape, tensegrity and transformation.

3-6
7 Dr A. G. McLennan
Revision of DNA replication in prokaryotes

Experimental approaches and model systems. The prokaryotic replication fork.

DNA replication in eukaryotes

Temporal and spatial organization within the nucleus. Replication foci. The nuclear matrix.

Eukaryotic origins of replication. Model systems: viruses, yeast, Xenopus.

Mechanisms of initiation. Initiation factors: ORC etc.

Enzymology of eukaryotic replication forks. DNA polymerases.

Chromatin replication. Telomere structure and replication. Mitochondrial and viral replicons.

Replication fidelity. Replication of damaged DNA. DNA damage and replication checkpoints in the cell cycle.

CONTENTS | NEXT PAGE

Location	Life Sciences LT1
Times	Wednesday: 09.00, Friday: 10.00

Week	Lecture	Content
1-3	5	Dr S. Pennington Introduction to the eukaryotic cell cycle Introduction. Major events and checkpoints of the cell cycle. Identification of the cell cycle machinery - the cyclins and cyclin-dependent kinases. Regulation of cell cycle checkpoints by cyclin-dependent kinases. Growth factor regulation of cell cycle progression. Cell shape, tensegrity and transformation.
3-6	7	Dr A. G. McLennan Revision of DNA replication in prokaryotes Experimental approaches and model systems. The prokaryotic replication fork. DNA replication in eukaryotes Temporal and spatial organization within the nucleus. Replication foci. The nuclear matrix. Eukaryotic origins of replication. Model systems: viruses, yeast, Xenopus. Mechanisms of initiation. Initiation factors: ORC etc. Enzymology of eukaryotic replication forks. DNA polymerases. Chromatin replication. Telomere structure and replication. Mitochondrial and viral replicons. Replication fidelity. Replication of damaged DNA. DNA damage and replication checkpoints in the cell cycle.