Samples

The Liverpool Bio-Innovation Hub (LBIH) Biobank collects diseased and adjacent non-involved tissue from a wide variety of tissue types, as well as clinical information, from patients who have undergone surgery at hospitals around the Merseyside region.

These include the following sites:

  • The Royal Liverpool and Broadgreen University Hospitals Trust
  • Aintree University Hospitals NHS Foundation Trust
  • Liverpool Women's Hospital NHS Foundation Trust
  • Liverpool Heart and Chest Hospital NHS Foundation Trust.

On average, the LBIH Biobank collects biosamples from over 800 donors per year. In total there are over 40,000 samples of paraffin wax embedded and frozen material. The largest collections are of breast, colorectal and pancreatic tissue.

We offer bespoke sample collecting. If you are interested in a tissue type that we do not have in our archives, we can discuss setting up a new collection.

Samples available to Researchers

The different type of biosamples provided by the LBIH Biobank is listed in the table below.

From Paraffin Wax Block Unstained Slide 5µm
  H&E Slide 5µm
  Tissue Block variable size
From Tissue Microarray Unstained slide 5µm
From Frozen Block Unstained Slide 5µm
  H&E Slide 5µm
  Tissue Block variable size
Biochemistry Samples (extracted from frozen blocks)

DNA

RNA

Protein

1µg

1µg

10µg

From Blood

Serum

Plasma

500µl

A number of Tissue Microarrays have been constructed from paraffin wax embedded tissue. Cores of tissue from multiple blocks from different cases can be placed onto a single recipient block to allow for the rapid staining of multiple cases. This provides an essential tool that can be utilised in a high throughput manner.

Tissue Type Number of cases
Breast 397 on 6 microarrays
Colorectal 360 on 6 microarrays
Fibroadenoma 83 on 1 microarray
Head and Neck 300 on 4 microarrays
Kidney 91 on 2 microarrays
Nasal Melanoma 20 on 1 microarrays
Pancreas 128 on 3 microarrays
Pancreatitis 48 on 2 microarrays

The LBIH Biobank operates a cost recovery scheme where a charge is made to researchers to cover the costs involved in the collection, storage and processing of biosamples. A quotation can be obtained from the LBIH Biobank Manager during the application process.

Data Information

Samples supplied to the researcher are pseudo-anonymised and the researcher can request clinical information, which includes gender, age at diagnosis, type and grade of tumour, pathological data such as hormone receptor status as well as clinical follow up and outcome data. Researchers utilising the samples will not be able to identify individual donors as patient identifiable information such as name, address, date of birth etc is released.

As part of the process of issuing biosamples and clinical information, researchers are asked to supply raw data obtained through the use of LBIH Biobank samples back to the LBIH Biobank following publication or completion of the research project. This is so that the LBIH Biobank can help in the direction of future research projects and promote collaboration between groups.

Quality Control

All biosamples undergo routine quality control checks before being issued. Frozen and paraffin wax embedded material have tumour or normal epithelium content verified and recorded by a pathologist based on a H&E slide. Both the slide and tissue report are available for review.