Assays

Analyte

Class

Matrix

Extraction Method

Internal Standard

Range (ng/mL)

Antiretrovirals - Plasma

Amprenavir

PI

Plasma

Protein precipitation

Quinoxaline

10 - 10,000

Atazanavir

PI

Plasma

Protein precipitation

Atazanvir-d5

10 - 10,000

Cobicistat

PK enhancer

Plasma

Liquid-liquid

Quinoxaline

10 - 4000

Darunavir

PI

Plasma

Protein precipitation

Darunavir-d9

15 - 15,000

Dolutegravir

INSTI

Plasma

Liquid-Liquid

Dolutegravir-d5

10 - 4000

Efavirenz

NNRTI

Plasma

Protein precipitation

13C6-Efavirenz

25 - 10,000

7-Hydroxy Efavirenz

NNRTI

Plasma

Protein precipitation

Hexobarbital

5 - 5,000

8-Hydroxy Efavirenz

NNRTI

Plasma

Protein precipitation

Hexobarbital

5 - 5,000

Elvitegravir

INSTI

Plasma

Liquid-liquid

Elvitegravir-d6

10 - 4000

Emtricitabine

NRTI

Plasma

Protein precipitation

2-chloroadenosine

5 - 5,000

Etraivrine

NNRTI

Plasma

Protein precipitation

Etravirine-d8

5 - 5,000

Lamivudine

NRTI

Plasma

Protein precipitation

2-chloroadenosine

5 - 5,000

Lopinavir

PI

Plasma

Protein precipitation

Lopinavir-d8

15 - 15,000

Maraviroc

CCR5 inhibitor

Plasma

Protein precipitation

Maraviroc-d6

2.5 - 2,500

Nevirapine

NNRTI

Plasma

Protein precipitation

Nevirapine-d5

10 - 10,000

Raltegravir

INSTI

Plasma

Protein precipitation

Quinoxaline

5 - 5,000

Rilpivirine

NNRTI

Plasma

Protein precipitation

13C-d4-rilpivirine

0.5 - 2000

Ritonavir

PI

Plasma

Protein precipitation

Ritonavir-d6

5 - 5,000

Saquinaivr

PI

Plasma

Protein precipitation

Quinoxaline

10 - 10,000

Tenofovir

NRTI

Plasma

Protein precipitation

2-chloroadenosine

5 - 5,000

Antiretrovirals – CSF

Amprenavir

PI

CSF

Protein precipitation

Quinoxaline

1.95 - 250

Atazanavir

PI                                

CSF

Protein precipitation

Atazanavir-d5

1.95 - 250

Darunavir

PI

CSF

Protein precipitation

Darunavir-d9

1.95 - 250

Efavirenz

NNRTI

CSF

Liquid-liquid

13C6-Efavirenz

0.5 - 2,500

7-Hydroxy Efavirenz

NNRTI

CSF

Liquid-liquid

13C6-Efavirenz

0.01 - 50

8-Hydroxy Efavirenz

NNRTI

CSF

Liquid-liquid

13C6-Efavirenz

0.05 - 250

Etravirine

NNRTI

CSF

Protein precipitation

Etravirine-d8

1.95 - 250

Lopinavir

PI

CSF

Protein precipitation

Lopinavir-d8

0.78 - 100

Maraviroc

CCR5 inhibitor

CSF

Protein precipitation

Maraviroc-d6

0.78 - 100

Nevirapine

NNRTI

CSF

Protein precipitation

Nevirapine-d5

19.53 - 2,500

Raltegravir

INSTI

CSF

Protein precipitation

Quinoxaline

1.95 - 250

Rilpivirine

NNRTI

CSF

Protein precipitation

13C-d4-rilpivirine

1.95 - 250

Ritonavir

PI

CSF

Protein precipitation

Ritonavir-d6

0.78 - 100

Antiretrovirals – PBMCs

Darunavir

PI

PBMC

Direct injection

Quinoxaline

0 - 150

Emtricitabine- Triphosphate

NRTI

PBMC

Direct injection

2-chloroadenosine 5’-triphosphate

0.5 - 100

Lamivudine- Triphosphate

NRTI

PBMC

Direct injection

2-chloroadenosine 5’-triphosphate

0.75 - 100

Lopinavir

PI

PBMC

Direct injection

Quinoxaline

0 - 150

Raltegravir

INSTI

PBMC

Direct injection

Quinoxaline

0 - 150

Ritonavir

PI

PBMC

Direct injection

Quinoxaline

0 - 150

Tenofovir-diphosphate

NRTI

PBMC

Direct injection

2-chloroadenosine 5’-triphosphate

0.5 - 100

Antiretrovirals – Urine

Amprenavir

PI

Urine

Protein precipitation

Quinoxaline

10 - 10,000

Atazanavir

PI

Urine

Protein precipitation

Quinoxaline

10 - 10,000

Darunavir

PI

Urine

Protein precipitation

Quinoxaline

4.9 - 5,000

Efavirenz

NNRTI

Urine

Protein precipitation

Quinoxaline

10 - 10,000

Emtricitabine

NRTI

Urine

Protein precipitation

2-chloroadenosine

12.5 - 5,000

Etravirine

NNRTI

Urine

Protein precipitation

Quinoxaline

4.9 - 5,000

Lamivudine

NRTI

Urine

Protein precipitation

Quinoxaline

12.5 - 5,000

Lopinavir

PI

Urine

Protein precipitation

Quinoxaline

14.8 - 15,000

Maraviroc

CCR5 inhibitor

Urine

Protein precipitation

Quinoxaline

2.5 - 2,500

Nevirapine

NNRTI

Urine

Protein precipitation

Quinoxaline

10 - 10,000

Raltegravir

INSTI

Urine

Protein precipitation

Quinoxaline

4.9 - 5,000

Rilpivirine

NNRTI

Urine

Protein precipitation

13C-d4-rilpivirine

4.9 - 5,000

Ritonavir

PI

Urine

Protein precipitation

Quinoxaline

4.9 - 5,000

Saquinavir

PI

Urine

Protein precipitation

Quinoxaline

10 - 10,000

Tenofovir

NRTI

Urine

Protein precipitation

2-chloroadenosine

12.5 - 5,000

Antiretrovirals – Other matrices

Emtricitabine

NRTI

DBMS

Protein precipitation

Emtricitabine-13C, 15N2

16.6 - 5000

Lamivudine

NRTI

DBMS

Protein precipitation

Lamivudine-d3

16.6 - 5000

Tenofovir

NRTI

DBMS

Protein precipitation

Emtricitabine-13C, 15N2

4.2 - 1250

Efavirenz

NNRTI

DBS

Liquid-liquid

Hexobarbital

25 - 5,000

Emtricitabine

NRTI

DBS

Protein precipitation

Emtricitabine-13C, 15N2

16.6 - 5000

Lamivudine

NRTI

DBS

Protein precipitation

Lamivudine-d3

16.6 - 5000

Tenofovir

NRTI

DBS

Protein precipitation

Emtricitabine-13C, 15N2

16.6 - 5000

Rilpivirine

NNRTI

CVF

Protein precipitation

13C-d4-rilpivrine

0 - 2000

Maraviroc

CCR5 inhibitor

CVF

Protein precipitation

Maraviroc-d6

0.25 - 10

Rilpivirine

NNRTI

CVL

Protein precipitation

13C-d4-rilpivirine

0 - 20

Maraviroc

CCR5 inhibitor

Saliva

Protein precipitation

Maraviroc-d6

0.25 - 10

Atazanavir

PI

Cellulose swabs

Liquid-liquid

Atazanavir-d5

0 - 75

Darunavir

PI

Cellulose swabs

Liquid-liquid

Darunavir-d9

0 - 300

Lopinavir

PI

Celluose swabs

Liquid-liquid

Quinoxaline

0 - 300

Maraviroc

CCR5 inihbitor

Cellulose swabs

Liquid-liquid

Maraviroc-d6

0 - 75

Rilpivirine

NNRTI

PVA swabs

Liquid-liquid

13C-d4-rilpivirine

0.025 - 20

Maraviroc

CCR5 inhibitor

Tissue biopsies

Protein precipitation

Maraviroc-d6

0 - 10

Rilpivirine

NNRTI

Tissue biopsies

Protein precipitation

13C-d4-rilpivirine

0.05 - 40

HCV drugs

Daclatasvir

NS5A inhibitor

Plasma

Liquid-liquid

Daclatasvir-d6

10 - 5,000

GS-331007

Sofosbuvir metabolite

Plasma

Liquid-liquid

13C2-d3- GS-331007

10 - 2,500

Ledipasvir

NS5A inhibitor

Plasma

Liquid-liquid

13C2-d6-Ledipasvir

10 - 2,500

Simepravir

NS3.4A PI

Plasma

Liquid-liquid

Simepravir-d3

10 - 5,000

Sofosbuvir

NS5B inhibitor

Plasma

Liquid-liquid

13C-d3-Sofosbuvir

2.5 - 1250

Telapravir + VRT127394

NS3.4A PI

Plasma

Liquid-liquid

Telaprevir-d11

5 - 5,000

Other analytes

Levonorgesterol

Steroid

Plasma

Liquid-liquid

Levonorgesterol (D-norgesterol)- d7

0.05 - 1.5

Sildenafil

PDE5 inhibitor

Plasma

Protein precipitation

Sildenafil-d8

1.1 - 1,045

Desmethyl Sildenfil

PDE5 inhibitor

Plasma

Protein precipitation

Desmethyl sildenafil-d8

0.5 - 514.8