The Liverpool Bioanalytical Facility

Assays

Assays
Analyte Class Matrix Extraction Method Internal Standard Range (ng/mL)
Antiretrovirals - Plasma
Amprenavir PI Plasma Protein precipitation Quinoxaline 10 - 10,000
Atazanavir PI Plasma Protein precipitation Atazanvir-d5 10 - 10,000
Bictegravir INSTI Plasma Protein precipitation Dolutegravir-d5 10 - 10,000
Cenicriviroc CCR5 inhibitor Plasma Protein precipitation Cenicriviroc-d7 5-1000
Cobicistat PK enhancer Plasma Liquid-liquid Quinoxaline 10-4000
Darunavir PI Plasma Protein precipitation Darunavir-d9 15 - 15,000
Dolutegravir INSTI Plasma Liquid-Liquid Dolutegravir-d5 10-4000
Efavirenz NNRTI Plasma Protein precipitation 13C6-Efavirenz 25 - 10,000
7-Hydroxy Efavirenz NNRTI Plasma Protein precipitation Hexobarbital 5 - 5,000
8-Hydroxy Efavirenz NNRTI Plasma Protein precipitation Hexobarbital 5 - 5,000
Elvitegravir INSTI Plasma Liquid-liquid Elvitegravir-d6 10-4000
Emtricitabine NRTI Plasma Protein precipitation 2-chloroadenosine 5 - 5,000
Etraivrine NNRTI Plasma Protein precipitation Etravirine-d8 5 - 5,000
Lamivudine NRTI Plasma Protein precipitation 2-chloroadenosine 5 - 5,000
Lopinavir PI Plasma Protein precipitation Lopinavir-d8 15 - 15,000
Maraviroc CCR5 inhibitor Plasma Protein precipitation Maraviroc-d6 2.5 - 2,500
Nevirapine NNRTI Plasma Protein precipitation Nevirapine-d5 10 - 10,000
Raltegravir INSTI Plasma Protein precipitation Quinoxaline 5 - 5,000
Rilpivirine NNRTI Plasma Protein precipitation 13C-d4-rilpivirine 0.5 - 2000
Ritonavir PI Plasma Protein precipitation Ritonavir-d6 5 - 5,000
Saquinaivr PI Plasma Protein precipitation Quinoxaline 10 - 10,000
Tenofovir NRTI Plasma Protein precipitation 2-chloroadenosine 5 - 5,000
Antiretrovirals – CSF
Amprenavir PI CSF Protein precipitation Quinoxaline 1.95 - 250
Atazanavir PI                                 CSF Protein precipitation Atazanavir-d5 1.95 - 250
Bictegravir INSTI CSF Protein precipitation Dolutegravir-d5 1.0 - 100
Cenicriviroc CCR5 inhibitor CSF Protein precipitation Cenicriviroc-d7 0.241-15
Darunavir PI CSF Protein precipitation Darunavir-d9 1.95 - 250
Efavirenz NNRTI CSF Liquid-liquid 13C6-Efavirenz 0.5 - 2,500
7-Hydroxy Efavirenz NNRTI CSF Liquid-liquid 13C6-Efavirenz 0.01 - 50
8-Hydroxy Efavirenz NNRTI CSF Liquid-liquid 13C6-Efavirenz 0.05 - 250
Etravirine NNRTI CSF Protein precipitation Etravirine-d8 1.95 - 250
Lopinavir PI CSF Protein precipitation Lopinavir-d8 0.78 - 100
Maraviroc CCR5 inhibitor CSF Protein precipitation Maraviroc-d6 0.78 - 100
Nevirapine NNRTI CSF Protein precipitation Nevirapine-d5 19.53 - 2,500
Raltegravir INSTI CSF Protein precipitation Raltegravir-d6 1.95 - 250
Rilpivirine NNRTI CSF Protein precipitation 13C-d4-rilpivirine 1.95 - 250
Ritonavir PI CSF Protein precipitation Ritonavir-d6 0.78 - 100
Antiretrovirals – PBMCs
Darunavir PI PBMC Direct injection Quinoxaline 0 - 150
Emtricitabine- Triphosphate NRTI PBMC Direct injection 2-chloroadenosine 5’-triphosphate 0.5 - 100
Lamivudine- Triphosphate NRTI PBMC Direct injection 2-chloroadenosine 5’-triphosphate 0.75 - 100
Lopinavir PI PBMC Direct injection Quinoxaline 0 - 150
Raltegravir INSTI PBMC Direct injection Quinoxaline 0 - 150
Ritonavir PI PBMC Direct injection Quinoxaline 0 - 150
Tenofovir-diphosphate NRTI PBMC Direct injection 2-chloroadenosine 5’-triphosphate 0.5 - 100
Antiretrovirals – Urine
Amprenavir PI Urine Protein precipitation Quinoxaline 10 - 10,000
Atazanavir PI Urine Protein precipitation Quinoxaline 10 - 10,000
Cobicistat PK enhancer Urine Direct injection Quinoxaline 10-4000
Darunavir PI Urine Protein precipitation Quinoxaline 4.9 - 5,000
Dolutegravir INSTI Urine Direct injection Dolutegravir-d5 10-4000
Efavirenz NNRTI Urine Protein precipitation Quinoxaline 10 - 10,000
Elvitegravir INSTI Urine Direct injection Elvitegravir-d6 10-4000
Emtricitabine NRTI Urine Protein precipitation 2-chloroadenosine 12.5 - 5,000
Etravirine NNRTI Urine Protein precipitation Quinoxaline 4.9 - 5,000
Lamivudine NRTI Urine Protein precipitation Quinoxaline 12.5 - 5,000
Lopinavir PI Urine Protein precipitation Quinoxaline 14.8 - 15,000
Maraviroc CCR5 inhibitor Urine Protein precipitation Quinoxaline 2.5 - 2,500
Nevirapine NNRTI Urine Protein precipitation Quinoxaline 10 - 10,000
Raltegravir INSTI Urine Protein precipitation Quinoxaline 4.9 - 5,000
Rilpivirine NNRTI Urine Protein precipitation 13C-d4-rilpivirine 4.9 - 5,000
Ritonavir PI Urine Protein precipitation Quinoxaline 4.9 - 5,000
Saquinavir PI Urine Protein precipitation Quinoxaline 10 - 10,000
Tenofovir NRTI Urine Protein precipitation 2-chloroadenosine 12.5 - 5,000
Antiretrovirals – Other matrices
Efavirenz NNRTI DBMS Protein precipitation Hexobarbital 50 - 6000
Emtricitabine NRTI DBMS Protein precipitation Emtricitabine-13C,15N2 16.6 - 5000
Dolutegravir INSTI DBMS Liquid-liquid Dolutegravir-d5 10 - 4000
Lamivudine NRTI DBMS Protein precipitation Lamivudine-d3 16.6 - 5000
Tenofovir NRTI DBMS Protein precipitation Emtricitabine-13C,15N2 4.2 - 1250
Efavirenz NNRTI DBS Liquid-liquid Hexobarbital 25 - 5,000
Emtricitabine NRTI DBS Protein precipitation Emtricitabine-13C,15N2 16.6 - 5000
Lamivudine NRTI DBS Protein precipitation Lamivudine-d3 16.6 - 5000
Tenofovir NRTI DBS Protein precipitation Emtricitabine-13C,15N2 16.6 - 5000
Rilpivirine NNRTI CVF Protein precipitation 13C-d4-rilpivrine 0 - 2000
Maraviroc CCR5 inhibitor CVF Protein precipitation Maraviroc-d6 0.25 - 10
Rilpivirine NNRTI CVL Protein precipitation 13C-d4-rilpivirine 0 - 20
Lamivudine NRTI Saliva Solid phase extraction Lamivudine 15N2 13C 0.5 - 200
Maraviroc CCR5 inhibitor Saliva Protein precipitation Maraviroc-d6 0.25 - 10
Raltegravir INSTI Saliva Solid phase extraction Raltegravir-d6 0.5 - 200
Atazanavir PI Cellulose swabs Liquid-liquid Atazanavir-d5 0 - 75
Darunavir PI Cellulose swabs Liquid-liquid Darunavir-d9 0 - 300
Lamivudine NRTI Cellulose swabs Solid phase extraction Lamivudine 15N2 13C 0.153 - 200
Lopinavir PI Celluose swabs Liquid-liquid Quinoxaline 0 - 300
Maraviroc CCR5 inihbitor Cellulose swabs Liquid-liquid Maraviroc-d6 0 - 75
Raltegravir INSTI Cellulose swabs Solid phase extraction Raltegravir-d6 0.153 - 200
Efavirenz NNRTI Cervicovaginal swabs Protein precipitation 13C6-Efavirenz 3.2 - 1088
Rilpivirine NNRTI PVA swabs Liquid-liquid 13C-d4-rilpivirine 0.025 - 20
Lamivudine NRTI Tissue biopsies Protein precipitation Lamivudine 15N2 13C 0.35 - 1000
Lamivudine triphosphate NRTI metabolite Tissue biopsies Protein precipitation Tenofovir-DP-13C6 0.35 - 1000
Maraviroc CCR5 inhibitor Tissue biopsies Protein precipitation Maraviroc-d6 0 - 10
Raltegravir INSTI Tissue biopsies Protein precipitation Raltegravir-d6 0.35 - 1000
Rilpivirine NNRTI Tissue biopsies Protein precipitation 13C-d4-rilpivirine 0.05 - 40
TB Drugs
Ethambutol  Antimycobacterial Plasma Protein precipitation Ethambutol-d4-dihydrochloride 7 - 3,500
Isoniazid  Antimycobacterial Plasma Protein precipitation Isoniazid-d4 20 - 10,000
Pyrazinamide  Antimycobacterial Plasma Protein precipitation Pyrazinamide-15N, d3 200 - 100,000
Rifampicin  Antimycobacterial Plasma Protein precipitation Rifampicin-d8 15 - 7,500
HCV drugs
Daclatasvir NS5A inhibitor Plasma Liquid-liquid Daclatasvir-d6 10 - 5,000
GS-331007 Sofosbuvir metabolite Plasma Liquid-liquid 13C2-d3- GS-331007 10 - 2,500
Ledipasvir NS5A inhibitor Plasma Liquid-liquid 13C2-d6-Ledipasvir 10 - 2,500
Simepravir NS3.4A PI Plasma Liquid-liquid Simepravir-d3 10 - 5,000
Sofosbuvir NS5B inhibitor Plasma Liquid-liquid 13C-d3-Sofosbuvir 2.5 - 1250
Telapravir + VRT127394 NS3.4A PI Plasma Liquid-liquid Telaprevir-d11 5 - 5,000
Other analytes
Acetylsalicylic acid NSAID Plasma Protein precipitation Acetylsalicylic acid-d3 50 - 5000
Ethinyl Estradiol Estrogen Plasma Liquid-liquid Ethinyl estradiol-d4 0.005-0.5
Levonorgesterol Steroid Plasma Liquid-liquid Levonorgesterol (D-norgesterol)- d7 0.05 - 1.5
Salicylic Acid NSAID  Plasma Protein precipitation Salicylic acid-d7 50 - 5000
Sildenafil PDE5 inhibitor Plasma Protein precipitation Sildenafil-d8 1.1 - 1,045
Desmethyl Sildenfil PDE5 inhibitor Plasma Protein precipitation Desmethyl sildenafil-d8 0.5 - 514.8