Assays

Analyte	Class	Matrix	Extraction Method	Internal Standard	Range (ng/mL)
Antiretrovirals - Plasma
Amprenavir	PI	Plasma	Protein precipitation	Quinoxaline	10 - 10,000
Atazanavir	PI	Plasma	Protein precipitation	Atazanvir-d5	10 - 10,000
Cobicistat	PK enhancer	Plasma	Liquid-liquid	Quinoxaline	10 - 4000
Darunavir	PI	Plasma	Protein precipitation	Darunavir-d9	15 - 15,000
Dolutegravir	INSTI	Plasma	Liquid-Liquid	Dolutegravir-d5	10 - 4000
Efavirenz	NNRTI	Plasma	Protein precipitation	¹³C₆-Efavirenz	25 - 10,000
7-Hydroxy Efavirenz	NNRTI	Plasma	Protein precipitation	Hexobarbital	5 - 5,000
8-Hydroxy Efavirenz	NNRTI	Plasma	Protein precipitation	Hexobarbital	5 - 5,000
Elvitegravir	INSTI	Plasma	Liquid-liquid	Elvitegravir-d6	10 - 4000
Emtricitabine	NRTI	Plasma	Protein precipitation	2-chloroadenosine	5 - 5,000
Etraivrine	NNRTI	Plasma	Protein precipitation	Etravirine-d8	5 - 5,000
Lamivudine	NRTI	Plasma	Protein precipitation	2-chloroadenosine	5 - 5,000
Lopinavir	PI	Plasma	Protein precipitation	Lopinavir-d8	15 - 15,000
Maraviroc	CCR5 inhibitor	Plasma	Protein precipitation	Maraviroc-d6	2.5 - 2,500
Nevirapine	NNRTI	Plasma	Protein precipitation	Nevirapine-d5	10 - 10,000
Raltegravir	INSTI	Plasma	Protein precipitation	Quinoxaline	5 - 5,000
Rilpivirine	NNRTI	Plasma	Protein precipitation	¹³C-d4-rilpivirine	0.5 - 2000
Ritonavir	PI	Plasma	Protein precipitation	Ritonavir-d6	5 - 5,000
Saquinaivr	PI	Plasma	Protein precipitation	Quinoxaline	10 - 10,000
Tenofovir	NRTI	Plasma	Protein precipitation	2-chloroadenosine	5 - 5,000
Antiretrovirals – CSF
Amprenavir	PI	CSF	Protein precipitation	Quinoxaline	1.95 - 250
Atazanavir	PI	CSF	Protein precipitation	Atazanavir-d5	1.95 - 250
Darunavir	PI	CSF	Protein precipitation	Darunavir-d9	1.95 - 250
Efavirenz	NNRTI	CSF	Liquid-liquid	¹³C₆-Efavirenz	0.5 - 2,500
7-Hydroxy Efavirenz	NNRTI	CSF	Liquid-liquid	¹³C₆-Efavirenz	0.01 - 50
8-Hydroxy Efavirenz	NNRTI	CSF	Liquid-liquid	¹³C₆-Efavirenz	0.05 - 250
Etravirine	NNRTI	CSF	Protein precipitation	Etravirine-d8	1.95 - 250
Lopinavir	PI	CSF	Protein precipitation	Lopinavir-d8	0.78 - 100
Maraviroc	CCR5 inhibitor	CSF	Protein precipitation	Maraviroc-d6	0.78 - 100
Nevirapine	NNRTI	CSF	Protein precipitation	Nevirapine-d5	19.53 - 2,500
Raltegravir	INSTI	CSF	Protein precipitation	Quinoxaline	1.95 - 250
Rilpivirine	NNRTI	CSF	Protein precipitation	¹³C-d4-rilpivirine	1.95 - 250
Ritonavir	PI	CSF	Protein precipitation	Ritonavir-d6	0.78 - 100
Antiretrovirals – PBMCs
Darunavir	PI	PBMC	Direct injection	Quinoxaline	0 - 150
Emtricitabine- Triphosphate	NRTI	PBMC	Direct injection	2-chloroadenosine 5’-triphosphate	0.5 - 100
Lamivudine- Triphosphate	NRTI	PBMC	Direct injection	2-chloroadenosine 5’-triphosphate	0.75 - 100
Lopinavir	PI	PBMC	Direct injection	Quinoxaline	0 - 150
Raltegravir	INSTI	PBMC	Direct injection	Quinoxaline	0 - 150
Ritonavir	PI	PBMC	Direct injection	Quinoxaline	0 - 150
Tenofovir-diphosphate	NRTI	PBMC	Direct injection	2-chloroadenosine 5’-triphosphate	0.5 - 100
Antiretrovirals – Urine
Amprenavir	PI	Urine	Protein precipitation	Quinoxaline	10 - 10,000
Atazanavir	PI	Urine	Protein precipitation	Quinoxaline	10 - 10,000
Darunavir	PI	Urine	Protein precipitation	Quinoxaline	4.9 - 5,000
Efavirenz	NNRTI	Urine	Protein precipitation	Quinoxaline	10 - 10,000
Emtricitabine	NRTI	Urine	Protein precipitation	2-chloroadenosine	12.5 - 5,000
Etravirine	NNRTI	Urine	Protein precipitation	Quinoxaline	4.9 - 5,000
Lamivudine	NRTI	Urine	Protein precipitation	Quinoxaline	12.5 - 5,000
Lopinavir	PI	Urine	Protein precipitation	Quinoxaline	14.8 - 15,000
Maraviroc	CCR5 inhibitor	Urine	Protein precipitation	Quinoxaline	2.5 - 2,500
Nevirapine	NNRTI	Urine	Protein precipitation	Quinoxaline	10 - 10,000
Raltegravir	INSTI	Urine	Protein precipitation	Quinoxaline	4.9 - 5,000
Rilpivirine	NNRTI	Urine	Protein precipitation	¹³C-d4-rilpivirine	4.9 - 5,000
Ritonavir	PI	Urine	Protein precipitation	Quinoxaline	4.9 - 5,000
Saquinavir	PI	Urine	Protein precipitation	Quinoxaline	10 - 10,000
Tenofovir	NRTI	Urine	Protein precipitation	2-chloroadenosine	12.5 - 5,000
Antiretrovirals – Other matrices
Emtricitabine	NRTI	DBMS	Protein precipitation	Emtricitabine-¹³C, ¹⁵N₂	16.6 - 5000
Lamivudine	NRTI	DBMS	Protein precipitation	Lamivudine-d3	16.6 - 5000
Tenofovir	NRTI	DBMS	Protein precipitation	Emtricitabine-¹³C, ¹⁵N₂	4.2 - 1250
Efavirenz	NNRTI	DBS	Liquid-liquid	Hexobarbital	25 - 5,000
Emtricitabine	NRTI	DBS	Protein precipitation	Emtricitabine-¹³C, ¹⁵N₂	16.6 - 5000
Lamivudine	NRTI	DBS	Protein precipitation	Lamivudine-d3	16.6 - 5000
Tenofovir	NRTI	DBS	Protein precipitation	Emtricitabine-¹³C, ¹⁵N₂	16.6 - 5000
Rilpivirine	NNRTI	CVF	Protein precipitation	¹³C-d4-rilpivrine	0 - 2000
Maraviroc	CCR5 inhibitor	CVF	Protein precipitation	Maraviroc-d6	0.25 - 10
Rilpivirine	NNRTI	CVL	Protein precipitation	¹³C-d4-rilpivirine	0 - 20
Maraviroc	CCR5 inhibitor	Saliva	Protein precipitation	Maraviroc-d6	0.25 - 10
Atazanavir	PI	Cellulose swabs	Liquid-liquid	Atazanavir-d5	0 - 75
Darunavir	PI	Cellulose swabs	Liquid-liquid	Darunavir-d9	0 - 300
Lopinavir	PI	Celluose swabs	Liquid-liquid	Quinoxaline	0 - 300
Maraviroc	CCR5 inihbitor	Cellulose swabs	Liquid-liquid	Maraviroc-d6	0 - 75
Rilpivirine	NNRTI	PVA swabs	Liquid-liquid	¹³C-d4-rilpivirine	0.025 - 20
Maraviroc	CCR5 inhibitor	Tissue biopsies	Protein precipitation	Maraviroc-d6	0 - 10
Rilpivirine	NNRTI	Tissue biopsies	Protein precipitation	¹³C-d4-rilpivirine	0.05 - 40
HCV drugs
Daclatasvir	NS5A inhibitor	Plasma	Liquid-liquid	Daclatasvir-d6	10 - 5,000
GS-331007	Sofosbuvir metabolite	Plasma	Liquid-liquid	¹³C₂-d3- GS-331007	10 - 2,500
Ledipasvir	NS5A inhibitor	Plasma	Liquid-liquid	¹³C₂-d6-Ledipasvir	10 - 2,500
Simepravir	NS3.4A PI	Plasma	Liquid-liquid	Simepravir-d3	10 - 5,000
Sofosbuvir	NS5B inhibitor	Plasma	Liquid-liquid	¹³C-d3-Sofosbuvir	2.5 - 1250
Telapravir + VRT127394	NS3.4A PI	Plasma	Liquid-liquid	Telaprevir-d11	5 - 5,000
Other analytes
Levonorgesterol	Steroid	Plasma	Liquid-liquid	Levonorgesterol (D-norgesterol)- d7	0.05 - 1.5
Sildenafil	PDE5 inhibitor	Plasma	Protein precipitation	Sildenafil-d8	1.1 - 1,045
Desmethyl Sildenfil	PDE5 inhibitor	Plasma	Protein precipitation	Desmethyl sildenafil-d8	0.5 - 514.8

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