Assays
|
Analyte |
Class |
Matrix |
Extraction Method |
Internal Standard |
Range (ng/mL) |
|
Antiretrovirals - Plasma |
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|
Amprenavir |
PI |
Plasma |
Protein precipitation |
Quinoxaline |
10 - 10,000 |
|
Atazanavir |
PI |
Plasma |
Protein precipitation |
Atazanvir-d5 |
10 - 10,000 |
|
Cobicistat |
PK enhancer |
Plasma |
Liquid-liquid |
Quinoxaline |
10 - 4000 |
|
Darunavir |
PI |
Plasma |
Protein precipitation |
Darunavir-d9 |
15 - 15,000 |
|
Dolutegravir |
INSTI |
Plasma |
Liquid-Liquid |
Dolutegravir-d5 |
10 - 4000 |
|
Efavirenz |
NNRTI |
Plasma |
Protein precipitation |
13C6-Efavirenz |
25 - 10,000 |
|
7-Hydroxy Efavirenz |
NNRTI |
Plasma |
Protein precipitation |
Hexobarbital |
5 - 5,000 |
|
8-Hydroxy Efavirenz |
NNRTI |
Plasma |
Protein precipitation |
Hexobarbital |
5 - 5,000 |
|
Elvitegravir |
INSTI |
Plasma |
Liquid-liquid |
Elvitegravir-d6 |
10 - 4000 |
|
Emtricitabine |
NRTI |
Plasma |
Protein precipitation |
2-chloroadenosine |
5 - 5,000 |
|
Etraivrine |
NNRTI |
Plasma |
Protein precipitation |
Etravirine-d8 |
5 - 5,000 |
|
Lamivudine |
NRTI |
Plasma |
Protein precipitation |
2-chloroadenosine |
5 - 5,000 |
|
Lopinavir |
PI |
Plasma |
Protein precipitation |
Lopinavir-d8 |
15 - 15,000 |
|
Maraviroc |
CCR5 inhibitor |
Plasma |
Protein precipitation |
Maraviroc-d6 |
2.5 - 2,500 |
|
Nevirapine |
NNRTI |
Plasma |
Protein precipitation |
Nevirapine-d5 |
10 - 10,000 |
|
Raltegravir |
INSTI |
Plasma |
Protein precipitation |
Quinoxaline |
5 - 5,000 |
|
Rilpivirine |
NNRTI |
Plasma |
Protein precipitation |
13C-d4-rilpivirine |
0.5 - 2000 |
|
Ritonavir |
PI |
Plasma |
Protein precipitation |
Ritonavir-d6 |
5 - 5,000 |
|
Saquinaivr |
PI |
Plasma |
Protein precipitation |
Quinoxaline |
10 - 10,000 |
|
Tenofovir |
NRTI |
Plasma |
Protein precipitation |
2-chloroadenosine |
5 - 5,000 |
|
Antiretrovirals – CSF |
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|
Amprenavir |
PI |
CSF |
Protein precipitation |
Quinoxaline |
1.95 - 250 |
|
Atazanavir |
PI |
CSF |
Protein precipitation |
Atazanavir-d5 |
1.95 - 250 |
|
Darunavir |
PI |
CSF |
Protein precipitation |
Darunavir-d9 |
1.95 - 250 |
|
Efavirenz |
NNRTI |
CSF |
Liquid-liquid |
13C6-Efavirenz |
0.5 - 2,500 |
|
7-Hydroxy Efavirenz |
NNRTI |
CSF |
Liquid-liquid |
13C6-Efavirenz |
0.01 - 50 |
|
8-Hydroxy Efavirenz |
NNRTI |
CSF |
Liquid-liquid |
13C6-Efavirenz |
0.05 - 250 |
|
Etravirine |
NNRTI |
CSF |
Protein precipitation |
Etravirine-d8 |
1.95 - 250 |
|
Lopinavir |
PI |
CSF |
Protein precipitation |
Lopinavir-d8 |
0.78 - 100 |
|
Maraviroc |
CCR5 inhibitor |
CSF |
Protein precipitation |
Maraviroc-d6 |
0.78 - 100 |
|
Nevirapine |
NNRTI |
CSF |
Protein precipitation |
Nevirapine-d5 |
19.53 - 2,500 |
|
Raltegravir |
INSTI |
CSF |
Protein precipitation |
Quinoxaline |
1.95 - 250 |
|
Rilpivirine |
NNRTI |
CSF |
Protein precipitation |
13C-d4-rilpivirine |
1.95 - 250 |
|
Ritonavir |
PI |
CSF |
Protein precipitation |
Ritonavir-d6 |
0.78 - 100 |
|
Antiretrovirals – PBMCs |
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|
Darunavir |
PI |
PBMC |
Direct injection |
Quinoxaline |
0 - 150 |
|
Emtricitabine- Triphosphate |
NRTI |
PBMC |
Direct injection |
2-chloroadenosine 5’-triphosphate |
0.5 - 100 |
|
Lamivudine- Triphosphate |
NRTI |
PBMC |
Direct injection |
2-chloroadenosine 5’-triphosphate |
0.75 - 100 |
|
Lopinavir |
PI |
PBMC |
Direct injection |
Quinoxaline |
0 - 150 |
|
Raltegravir |
INSTI |
PBMC |
Direct injection |
Quinoxaline |
0 - 150 |
|
Ritonavir |
PI |
PBMC |
Direct injection |
Quinoxaline |
0 - 150 |
|
Tenofovir-diphosphate |
NRTI |
PBMC |
Direct injection |
2-chloroadenosine 5’-triphosphate |
0.5 - 100 |
|
Antiretrovirals – Urine |
|||||
|
Amprenavir |
PI |
Urine |
Protein precipitation |
Quinoxaline |
10 - 10,000 |
|
Atazanavir |
PI |
Urine |
Protein precipitation |
Quinoxaline |
10 - 10,000 |
|
Darunavir |
PI |
Urine |
Protein precipitation |
Quinoxaline |
4.9 - 5,000 |
|
Efavirenz |
NNRTI |
Urine |
Protein precipitation |
Quinoxaline |
10 - 10,000 |
|
Emtricitabine |
NRTI |
Urine |
Protein precipitation |
2-chloroadenosine |
12.5 - 5,000 |
|
Etravirine |
NNRTI |
Urine |
Protein precipitation |
Quinoxaline |
4.9 - 5,000 |
|
Lamivudine |
NRTI |
Urine |
Protein precipitation |
Quinoxaline |
12.5 - 5,000 |
|
Lopinavir |
PI |
Urine |
Protein precipitation |
Quinoxaline |
14.8 - 15,000 |
|
Maraviroc |
CCR5 inhibitor |
Urine |
Protein precipitation |
Quinoxaline |
2.5 - 2,500 |
|
Nevirapine |
NNRTI |
Urine |
Protein precipitation |
Quinoxaline |
10 - 10,000 |
|
Raltegravir |
INSTI |
Urine |
Protein precipitation |
Quinoxaline |
4.9 - 5,000 |
|
Rilpivirine |
NNRTI |
Urine |
Protein precipitation |
13C-d4-rilpivirine |
4.9 - 5,000 |
|
Ritonavir |
PI |
Urine |
Protein precipitation |
Quinoxaline |
4.9 - 5,000 |
|
Saquinavir |
PI |
Urine |
Protein precipitation |
Quinoxaline |
10 - 10,000 |
|
Tenofovir |
NRTI |
Urine |
Protein precipitation |
2-chloroadenosine |
12.5 - 5,000 |
|
Antiretrovirals – Other matrices |
|||||
|
Emtricitabine |
NRTI |
DBMS |
Protein precipitation |
Emtricitabine-13C, 15N2 |
16.6 - 5000 |
|
Lamivudine |
NRTI |
DBMS |
Protein precipitation |
Lamivudine-d3 |
16.6 - 5000 |
|
Tenofovir |
NRTI |
DBMS |
Protein precipitation |
Emtricitabine-13C, 15N2 |
4.2 - 1250 |
|
Efavirenz |
NNRTI |
DBS |
Liquid-liquid |
Hexobarbital |
25 - 5,000 |
|
Emtricitabine |
NRTI |
DBS |
Protein precipitation |
Emtricitabine-13C, 15N2 |
16.6 - 5000 |
|
Lamivudine |
NRTI |
DBS |
Protein precipitation |
Lamivudine-d3 |
16.6 - 5000 |
|
Tenofovir |
NRTI |
DBS |
Protein precipitation |
Emtricitabine-13C, 15N2 |
16.6 - 5000 |
|
Rilpivirine |
NNRTI |
CVF |
Protein precipitation |
13C-d4-rilpivrine |
0 - 2000 |
|
Maraviroc |
CCR5 inhibitor |
CVF |
Protein precipitation |
Maraviroc-d6 |
0.25 - 10 |
|
Rilpivirine |
NNRTI |
CVL |
Protein precipitation |
13C-d4-rilpivirine |
0 - 20 |
|
Maraviroc |
CCR5 inhibitor |
Saliva |
Protein precipitation |
Maraviroc-d6 |
0.25 - 10 |
|
Atazanavir |
PI |
Cellulose swabs |
Liquid-liquid |
Atazanavir-d5 |
0 - 75 |
|
Darunavir |
PI |
Cellulose swabs |
Liquid-liquid |
Darunavir-d9 |
0 - 300 |
|
Lopinavir |
PI |
Celluose swabs |
Liquid-liquid |
Quinoxaline |
0 - 300 |
|
Maraviroc |
CCR5 inihbitor |
Cellulose swabs |
Liquid-liquid |
Maraviroc-d6 |
0 - 75 |
|
Rilpivirine |
NNRTI |
PVA swabs |
Liquid-liquid |
13C-d4-rilpivirine |
0.025 - 20 |
|
Maraviroc |
CCR5 inhibitor |
Tissue biopsies |
Protein precipitation |
Maraviroc-d6 |
0 - 10 |
|
Rilpivirine |
NNRTI |
Tissue biopsies |
Protein precipitation |
13C-d4-rilpivirine |
0.05 - 40 |
|
HCV drugs |
|||||
|
Daclatasvir |
NS5A inhibitor |
Plasma |
Liquid-liquid |
Daclatasvir-d6 |
10 - 5,000 |
|
GS-331007 |
Sofosbuvir metabolite |
Plasma |
Liquid-liquid |
13C2-d3- GS-331007 |
10 - 2,500 |
|
Ledipasvir |
NS5A inhibitor |
Plasma |
Liquid-liquid |
13C2-d6-Ledipasvir |
10 - 2,500 |
|
Simepravir |
NS3.4A PI |
Plasma |
Liquid-liquid |
Simepravir-d3 |
10 - 5,000 |
|
Sofosbuvir |
NS5B inhibitor |
Plasma |
Liquid-liquid |
13C-d3-Sofosbuvir |
2.5 - 1250 |
|
Telapravir + VRT127394 |
NS3.4A PI |
Plasma |
Liquid-liquid |
Telaprevir-d11 |
5 - 5,000 |
|
Other analytes |
|||||
|
Levonorgesterol |
Steroid |
Plasma |
Liquid-liquid |
Levonorgesterol (D-norgesterol)- d7 |
0.05 - 1.5 |
|
Sildenafil |
PDE5 inhibitor |
Plasma |
Protein precipitation |
Sildenafil-d8 |
1.1 - 1,045 |
|
Desmethyl Sildenfil |
PDE5 inhibitor |
Plasma |
Protein precipitation |
Desmethyl sildenafil-d8 |
0.5 - 514.8 |