800, 700 and 600 MHz NMR Spectrometers

A broad range of analytical services

The NMR centre for structural biology offers solution and solid-state NMR within one laboratory. Investment by the Technology Directorate into NMR as a shared research facility has provided auto-sampling and liquid handling capabilities to complement the existing state-of-the-art equipment. Furthermore a dedicated NMR technologist is on hand to ensure Shared Research Facility users obtain the best quality data from the most appropriate experimental design.

NMR centre equipment includes:

  • 800 MHz spectrometer with high sensitivity cryogenic heads for solution studies
  • 700 MHz spectrometer with high sensitivity cryogenic heads for solution studies
  • 600 MHz spectrometer with high sensitivity cryogenic heads for solution studies
  • Microcal ITC200 isothermal titration calorimetry
  • Biacore X100 surface plasmon resonance
  • Cary 400 fluorescence spectrophotometer
  • Cary Eclipse UV-Vis spectrophotometer
  • Bruker SampleJet metabolomics autoinjector.

Typical expertise of the centre includes:

  • Determination of protein structures
  • Investigation of protein-ligand interactions
  • Use of biophysical techniques in small molecule inhibitor design and screening
  • Metabolite analysis and characterisation
  • Recombinant protein expression and purification
  • Biophysical investigations using isothermal titration calorimetry, surface plasmon resonance and fluorescence, enzyme assays.