Photo of Dr Violaine See

Dr Violaine See Ph.D.

Lecturer Molecular Physiology & Cell Signalling

    Research

    Cell signalling in hypoxia

    Glioblastoma cells cultured as spheroids and imaged with a fluorescent light sheet microscope
    Glioblastoma cells cultured as spheroids and imaged with a fluorescent light sheet microscope

    My research focuses on the cellular response to environmental O2 level variations and their impact on cell fate. As the environment in solid tumours is hypoxic, or subject to episodes of hypoxia and re-oxygenation, my research has direct significance to cancer. I work across scales from single molecule to small organism, using advanced imaging technologies to elucidate the role of hypoxia on cancer cell phenotype. Building on collaborations with leading national clinical institutions (the Alder Hey children hospital and the Clatterbridge Cancer Centre), I have concentrated on the effects of hypoxia in paediatric (neuroblastoma, medulloblastoma) and brain tumours (glioblastoma).

    Research Grants

    Evaluation of novel combination drug protocols for neuroblastoma using advanced imaging in a chick embryo model

    ALDER HEY CHILDREN'S CHARITY (UK), NORTH WEST CANCER RESEARCH INCORPORATING CLATTERBRIDGE CANCER RESEARCH (UK)

    January 2018 - May 2021

    Understanding Drug Resistance in Paediatric and Adult Brain Tumours

    ALDER HEY CHILDREN'S CHARITY (UK)

    October 2018 - December 2018

    A multimodal imaging platform with real-time super-resolution for research across the life sciences

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL

    May 2018 - April 2019

    The chick embryo – A new versatile experimental model to study the biology of Neuroblastoma and targeted therapies

    ROYAL COLLEGE OF SURGEONS OF ENGLAND(UK)

    February 2017 - January 2018

    The chick embryo – A new versatile experimental model to study the biology of Neuroblastoma and targeted therapies

    ALDER HEY CHILDREN'S CHARITY (UK)

    October 2016 - March 2018

    2014 allocation - Wellcome ISSF non clinical fellowships

    WELLCOME TRUST (UK)

    May 2015 - October 2018

    Development of New Mathematical Sciences Capabilities for Healthcare Technologies

    ENGINEERING & PHYSICAL SCIENCES RESEARCH COUNCIL

    December 2015 - April 2021

    Bench fees for Yousef Al-Mutawa MPhil

    THE CULTURAL OFFICE OF THE EMBASSY OF THE STATE OF KUWAIT (UK)

    September 2015 - August 2016

    Mechanisms of multidrug resistance proteins transcription in neuroblastoma cells

    ALDER HEY CHILDREN'S NHS FOUNDATION TRUST (UK)

    November 2010 - October 2012

    Mechanism and consequences of MYCN down regulation in neuroblastoma cells in vivo and in vitro

    NEUROBLASTOMA (UK)

    October 2012 - April 2014

    How does the embryonic environment control MYCN expression in neuroblastoma cells?

    NEUROBLASTOMA RESEARCH FUND (UK)

    May 2012 - May 2013

    University of Liverpool Institutional Discipline Bridging.

    MEDICAL RESEARCH COUNCIL

    October 2003 - October 2006

    Establishment of a lent viral system

    ALDER HEY CHILDREN'S CHARITY (UK)

    January 2014 - February 2014

    Liverpool BioAFM: an integrated optical and atomic force microscope for research across the life sciences

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL

    January 2015 - January 2016

    Neuroblastoma Molecular Research

    ALDER HEY CHILDREN'S NHS FOUNDATION TRUST (UK)

    November 2007 - October 2009

    Live cell imaging and system biology approaches to understand the role of hypoxia-dependent signalling in brain tumour aggressiveness

    NASEEM'S MANX BRAIN TUMOUR CHARITY (ISLE OF MAN)

    October 2012 - September 2016

    Elucidation of non-coding miRNAs transcription in medulloblastoma and their role in response to chemotherapeutic treatment.

    ALDER HEY CHILDREN'S NHS FOUNDATION TRUST (UK)

    January 2010 - June 2013

    Exploiting the embryonic environment to reprogram cancer stem cells in neuroblastoma

    NEUROBLASTOMA (UK)

    October 2008 - September 2012

    Molecular and cellular understanding of AT/RT to improve therapeutic outcomes

    ALDER HEY CHILDREN'S NHS FOUNDATION TRUST (UK)

    October 2010 - September 2012

    CIC bioGUNE / University of Liverpool PhD programme for 2012/13

    CIC BIOGUNE (SPAIN)

    October 2012 - December 2016

    Dynamics and function of the NF-kappaB signalling system

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL

    May 2008 - July 2014

    Advanced in vivo imaging and transcriptomic analysis of neuroblastoma metastasis in a chick embryo model

    NEUROBLASTOMA (UK)

    October 2014 - February 2018

    From calcium to gene transcription: elucidation of spatio-temporal encoding of intracellular signals in neuronal cells.

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL

    September 2005 - January 2011

    Live monitoring of cancer cell migration and invasion in an in vivo chick embryo model

    CANCER RESEARCH UK (UK)

    April 2012 - March 2013

    Imaging of molecular dynamics and cell fate to identify new targets for Medulloblastoma therapy

    ALDER HEY CHILDREN'S NHS FOUNDATION TRUST (UK)

    November 2009 - May 2010

    Fluorescence Light Sheet Microscopy for Live 3D and 4D imaging

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL

    January 2014 - June 2014

    Role of Nibrin in medulloblastoma sensitivity to chemo-and radiotherapy and its potential as a prognostic marker for medulloblastoma patients.

    ALDER HEY CHILDREN'S CHARITY (UK)

    October 2014 - September 2018

    Exploiting apoptotic regulation of normal neural development to force cell death in refractory neuroblastoma

    NEUROBLASTOMA (UK)

    January 2007 - December 2009

    Role of hypoxia in paediatric tumour cells migration, adhesion and invasion

    ALDER HEY CHILDREN'S NHS FOUNDATION TRUST (UK)

    June 2013 - September 2014

    Developing a collaborative resource for ChIP based analyses of cancer related transcriptional networks

    CANCER RESEARCH UK (UK)

    April 2012 - March 2013

    Liverpool Imaging Partnership: Molecular physiology and drug response

    MEDICAL RESEARCH COUNCIL

    February 2013 - February 2017

    A systems biology study of E2F and NF-kappaB cross-talk

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL

    February 2010 - April 2014

    Development of live cell imaging from single cells to single molecules

    BIOTECHNOLOGY & BIOLOGICAL SCIENCE RESEARCH COUNCIL

    September 2007 - August 2010

    Imaging of molecular dynamics and cell fate to identify new targets for Medullobladtoma therapy

    SAMANTHA DICKSON BRAIN TUMOUR TRUST (UK)

    November 2006 - November 2009

    Research Collaborations

    D Moss

    Internal

    injection of cancer cells into a chick embryo model

    Daniel Stiehl

    External: University of Zurich

    M Brust

    Internal

    fate of gold nanoparticles inside living cells

    R Bearon

    Internal

    mathematical modelling of hypoxia dependent signalling pathway

    S Edwards

    Internal

    role and dynamic localisation of Mcl-1 in brain tumours

    M Cross

    Internal

    calcium measurement in endothelial cells

    R Wilson

    Internal

    Q-dots delivery and imaging in living cells

    C Giovannangelli

    External: Natural History Museum

    design of triplex forming oligonucleotides for gold nanoparticles

    Dr AL Boutillier

    External: Universite Louis Pasteur

    chromatin remodeling during neuronal differentiation

    B. Pizer

    External: Alder Hey Hospital

    Intracellular signalling in neuroblastoma and medulloblastoma

    H. Mc Dowell

    External: Alder Hey Hospital

    intracellular signalling in neuroblastoma and medulloblastoma

    R Levy

    Internal

    imaging of nanoparticules in living cells

    M. Faria

    External: Institut Quimica, Sao-Paulo

    calcium measurement during neuronl differentiation

    H. Bito

    External: The University of Tokyo

    calcium-dependent signalling in primary neurons