Dr James Johnson BSc. PhD.

Postdoctoral Research Associate Biochemistry & Systems Biology

    Publications

    2021

    Synthetic biology meets proteomics: Construction of a la carte QconCATs for absolute protein quantification (Journal article)

    Johnson, J., Harman, V., de Matos Ferraz Franco, C., Rockliffe, N., Sun, Y., Liu, L., . . . Beynon, R. (2021). Synthetic biology meets proteomics: Construction of a la carte QconCATs for absolute protein quantification. doi:10.1101/2021.04.13.439592

    DOI: 10.1101/2021.04.13.439592

    2019

    PET hydrolysing enzymes catalyse bioplastics precursor synthesis under aqueous conditions (Journal article)

    Parisi, D., Riley, C., Srivastava, A. S., McCue, H. V., Johnson, J. R., & Carnell, A. J. (2019). PET hydrolysing enzymes catalyse bioplastics precursor synthesis under aqueous conditions. GREEN CHEMISTRY, 21(14), 3827-3833. doi:10.1039/c9gc01284b

    DOI: 10.1039/c9gc01284b

    Building a global alliance of biofoundries. (Journal article)

    Hillson, N., Caddick, M., Cai, Y., Carrasco, J. A., Chang, M. W., Curach, N. C., . . . Freemont, P. S. (2019). Building a global alliance of biofoundries (vol 10, 2040, 2019). NATURE COMMUNICATIONS, 10. doi:10.1038/s41467-019-10862-1

    DOI: 10.1038/s41467-019-10862-1

    Building a global alliance of biofoundries (Journal article)

    Hillson, N., Caddick, M., Cai, Y., Carrasco, J. A., Chang, M. W., Curach, N. C., . . . Freemont, P. S. (2019). Building a global alliance of biofoundries. NATURE COMMUNICATIONS, 10. doi:10.1038/s41467-019-10079-2

    DOI: 10.1038/s41467-019-10079-2

    2017

    Ethanol Stimulates Locomotion via a G(alpha s)-Signaling Pathway in IL2 Neurons in Caenorhabditis elegans (Journal article)

    Johnson, J. R., Edwards, M. R., Davies, H., Newman, D., Holden, W., Jenkins, R. E., . . . Barclay, J. W. (2017). Ethanol Stimulates Locomotion via a G(alpha s)-Signaling Pathway in IL2 Neurons in Caenorhabditis elegans. GENETICS, 207(3), 1023-1039. doi:10.1534/genetics.117.300119

    DOI: 10.1534/genetics.117.300119

    Leaf LIMS: A Flexible Laboratory Information Management System with a Synthetic Biology Focus. (Journal article)

    Craig, T., Holland, R., D'Amore, R., Johnson, J. R., McCue, H. V., West, A., . . . Caddick, M. X. (2017). Leaf LIMS: A Flexible Laboratory Information Management System with a Synthetic Biology Focus.. ACS synthetic biology, 6(12), 2273-2280. doi:10.1021/acssynbio.7b00212

    DOI: 10.1021/acssynbio.7b00212

    arktic & mosquito: automated high-density sample storage and miniaturised liquid handling for high-throughput synthetic biology construct assembly (Media)

    Johnson, J. R. (n.d.). arktic & mosquito: automated high-density sample storage and miniaturised liquid handling for high-throughput synthetic biology construct assembly. [Application Note].

    SMRT Gate: A method for validation of synthetic constructs on Pacific Biosciences sequencing platforms (Journal article)

    D'Amore, R., Johnson, J., Haldenby, S., Hall, N., Hughes, M., Joynson, R., . . . Hall, A. (2017). SMRT Gate: A method for validation of synthetic constructs on Pacific Biosciences sequencing platforms. BIOTECHNIQUES, 63(1), 13-20. doi:10.2144/000114565

    DOI: 10.2144/000114565

    2016

    GeneMill: A 21st century platform for innovation (Journal article)

    Johnson, J. R., D'Amore, R., Thain, S. C., Craig, T., Mccue, H. V., Hertz-Fowler, C., . . . Hall, A. J. W. (2016). GeneMill: A 21st century platform for innovation. BIOCHEMICAL SOCIETY TRANSACTIONS, 44, 681-683. doi:10.1042/BST20160012

    DOI: 10.1042/BST20160012

    Small Heat Shock Proteins Are Novel Common Determinants of Alcohol and Nicotine Sensitivity in Caenorhabditis elegans (Journal article)

    Johnson, J. R., Rajamanoharan, D., McCue, H. V., Rankin, K., & Barclay, J. W. (2016). Small Heat Shock Proteins Are Novel Common Determinants of Alcohol and Nicotine Sensitivity in Caenorhabditis elegans. Genetics, 202(3), 1013-1027. doi:10.1534/genetics.115.185025

    DOI: 10.1534/genetics.115.185025

    2014

    Caenorhabditis elegans dnj-14, the orthologue of the DNAJC5 gene mutated in adult onset neuronal ceroid lipofuscinosis, provides a new platform for neuroprotective drug screening and identifies a SIR-2.1-independent action of resveratrol (Journal article)

    Kashyap, S. S., Johnson, J. R., McCue, H., Chen, X., Edmonds, M. J., Ayala, M., . . . Morgan, A. (2014). Caenorhabditis elegans dnj-14, the orthologue of the DNAJC5 gene mutated in adult onset neuronal ceroid lipofuscinosis, provides a new platform for neuroprotective drug screening and identifies a SIR-2.1-independent action of resveratrol. Human Molecular Genetics, 23(22), 5916-5927. doi:10.1093/hmg/ddu316

    DOI: 10.1093/hmg/ddu316

    2013

    Rab-3 and unc-18 Interactions in Alcohol Sensitivity Are Distinct from Synaptic Transmission (Journal article)

    Johnson, J. R., Kashyap, S., Rankin, K., & Barclay, J. W. (2013). Rab-3 and unc-18 Interactions in Alcohol Sensitivity Are Distinct from Synaptic Transmission. PLOS ONE, 8(11). doi:10.1371/journal.pone.0081117

    DOI: 10.1371/journal.pone.0081117

    Identification of key structural elements for neuronal calcium sensor-1 function in the regulation of the temperature-dependency of locomotion in C. elegans (Journal article)

    Martin, V. M., Johnson, J. R., Haynes, L. P., Barclay, J. W., & Burgoyne, R. D. (2013). Identification of key structural elements for neuronal calcium sensor-1 function in the regulation of the temperature-dependency of locomotion in C. elegans. MOLECULAR BRAIN, 6. doi:10.1186/1756-6606-6-39

    DOI: 10.1186/1756-6606-6-39

    2012

    NMR Structure of Hsp12, a Protein Induced by and Required for Dietary Restriction-Induced Lifespan Extension in Yeast (Journal article)

    Herbert, A. P., Riesen, M., Bloxam, L., Kosmidou, E., Wareing, B. M., Johnson, J. R., . . . Morgan, A. (2012). NMR Structure of Hsp12, a Protein Induced by and Required for Dietary Restriction-Induced Lifespan Extension in Yeast. PLOS ONE, 7(7). doi:10.1371/journal.pone.0041975

    DOI: 10.1371/journal.pone.0041975

    PKC-2 Phosphorylation of UNC-18 Ser322 in AFD Neurons Regulates Temperature Dependency of Locomotion (Journal article)

    Edwards, M. R., Johnson, J. R., Rankin, K., Jenkins, R. E., Maguire, C., Morgan, A., . . . Barclay, J. W. (2012). PKC-2 Phosphorylation of UNC-18 Ser322 in AFD Neurons Regulates Temperature Dependency of Locomotion. JOURNAL OF NEUROSCIENCE, 32(20), 7042-7051. doi:10.1523/JNEUROSCI.4029-11.2012

    DOI: 10.1523/JNEUROSCI.4029-11.2012

    2011

    Cell Cycle Phase Regulates Glucocorticoid Receptor Function (Journal article)

    Matthews, L., Johnson, J., Berry, A., Trebble, P., Cookson, A., Spiller, D., . . . Ray, D. (2011). Cell Cycle Phase Regulates Glucocorticoid Receptor Function. PLOS ONE, 6(7). doi:10.1371/journal.pone.0022289

    DOI: 10.1371/journal.pone.0022289

    Structure-Function Study of Mammalian Munc18-1 and C. elegans UNC-18 Implicates Domain 3b in the Regulation of Exocytosis (Journal article)

    Graham, M. E., Prescott, G. R., Johnson, J. R., Jones, M., Walmesley, A., Haynes, L. P., . . . Barclay, J. W. (2011). Structure-Function Study of Mammalian Munc18-1 and C. elegans UNC-18 Implicates Domain 3b in the Regulation of Exocytosis. PLOS ONE, 6(3). doi:10.1371/journal.pone.0017999

    DOI: 10.1371/journal.pone.0017999

    2010

    Caenorhabditis elegans: a useful tool to decipher neurodegenerative pathways (Journal article)

    Johnson, J. R., Jenn, R. C., Barclay, J. W., Burgoyne, R. D., & Morgan, A. (2010). Caenorhabditis elegans: a useful tool to decipher neurodegenerative pathways. BIOCHEMICAL SOCIETY TRANSACTIONS, 38, 559-563. doi:10.1042/BST0380559

    DOI: 10.1042/BST0380559

    Presynaptic targets for acute ethanol sensitivity (Journal article)

    Barclay, J. W., Graham, M. E., Edwards, M. R., Johnson, J. R., Morgan, A., & Burgoyne, R. D. (2010). Presynaptic targets for acute ethanol sensitivity. BIOCHEMICAL SOCIETY TRANSACTIONS, 38, 172-176. doi:10.1042/BST0380172

    DOI: 10.1042/BST0380172

    2009

    Binding of UNC-18 to the N-terminus of syntaxin is essential for neurotransmission in Caenorhabditis elegans (Journal article)

    Johnson, J. R., Ferdek, P., Lian, L. -Y., Barclay, J. W., Burgoyne, R. D., & Morgan, A. (2009). Binding of UNC-18 to the N-terminus of syntaxin is essential for neurotransmission in Caenorhabditis elegans. BIOCHEMICAL JOURNAL, 418, 73-80. doi:10.1042/BJ20081956

    DOI: 10.1042/BJ20081956

    2008

    S-nitrosylation of syntaxin 1 at Cys(145) is a regulatory switch controlling Munc18-1 binding (Journal article)

    Palmer, Z. J., Duncan, R. R., Johnson, J. R., Lian, L. -Y., Mello, L. V., Booth, D., . . . Morgan, A. (2008). S-nitrosylation of syntaxin 1 at Cys(145) is a regulatory switch controlling Munc18-1 binding. BIOCHEMICAL JOURNAL, 413, 479-491. doi:10.1042/BJ20080069

    DOI: 10.1042/BJ20080069

    2006

    Automatic tracking of biological cells and compartments using particle filters and active contours (Journal article)

    Shen, H., Nelson, G., Kennedy, S., Nelson, D., Johnson, J., Spiller, D., . . . Kell, D. B. (2006). Automatic tracking of biological cells and compartments using particle filters and active contours. CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS, 82(1-2), 276-282. doi:10.1016/j.chemolab.2005.07.007

    DOI: 10.1016/j.chemolab.2005.07.007

    2005

    BCG INHIBITION OF MURINE LEUKEMIA - LOCAL SUPPRESSION AND SYSTEMIC TUMOR IMMUNITY REQUIRE DIFFERENT DOSES (Journal article)

    BARTLETT, G. L., PURNELL, D. M., & KREIDER, J. W. (1976). BCG INHIBITION OF MURINE LEUKEMIA - LOCAL SUPPRESSION AND SYSTEMIC TUMOR IMMUNITY REQUIRE DIFFERENT DOSES. SCIENCE, 191(4224), 299-301. doi:10.1126/science.1108198

    DOI: 10.1126/science.1108198

    2004

    Oscillations in NF-kappa B signaling control the dynamics of gene expression (Journal article)

    Nelson, D. E., Ihekwaba, A. E. C., Elliott, M., Johnson, J. R., Gibney, C. A., Foreman, B. E., . . . White, M. R. H. (2004). Oscillations in NF-kappa B signaling control the dynamics of gene expression. SCIENCE, 306(5696), 704-708. doi:10.1126/science.1099962

    DOI: 10.1126/science.1099962

    2002

    Phytanic acid, a natural peroxisome proliferator-activated receptor agonist, regulates glucose metabolism in rat primary hepatocytes (Journal article)

    Heim, M., Johnson, J., Boess, F., Bendik, I., Weber, P., Hunziker, W., & Fluhmann, B. (2002). Phytanic acid, a natural peroxisome proliferator-activated receptor agonist, regulates glucose metabolism in rat primary hepatocytes. FASEB JOURNAL, 16(3), 718-+. doi:10.1096/fj.01-0816fje

    DOI: 10.1096/fj.01-0816fje