Using embryonic stem cell models to determine the importance of post transcriptional gene regulation in the early stages of embryo development

Description

This opportunity will close once a successful applicant has been appointed.

This project will examine how regulators of gene expression at the post transcriptional level, affect the very earliest processes of embryo development. These processes are challenging to track in actual embryos, so our group uses a 3D organoid model using embryonic stem cells, called gastruloids, which closely mimic a number of the critical processes that control development of the early embryo. By using embryonic stem cells derived from mice that have important post-transcriptional gene regulators knocked out, the project will be able to identify how embryo development is altered and determine the mechanisms that underpin this.

Training will be provided in a range of embryonic stem cell culture techniques, including cell derivation, maintenance and organoid culture. Further training in a full suite of techniques to analyse cell signalling (including gene expression and in situ hybridisation) and transcriptome wide gene expression (RNA-Seq) will be provided to achieve the aims of the study. The project would suit a candidate who has an interest in the stem cells, genetics or developmental biology.

To learn more about the work in our laboratories visit our websites:

https://www.liverpool.ac.uk/ageing-and-chronic-disease/staff/simon-tew/

https://www.liverpool.ac.uk/ageing-and-chronic-disease/staff/david-turner/

https://www.liverpool.ac.uk/ageing-and-chronic-disease/staff/rachel-oldershaw/

The Institute of Ageing and Chronic Disease is fully committed to promoting gender equality in all activities. In recruitment we emphasize the supportive nature of the working environment and the flexible family support that the University provides. The Institute holds a silver Athena SWAN award in recognition of on-going commitment to ensuring that the Athena SWAN principles are embedded in its activities and strategic initiatives. 

Informal enquiries to Dr Simon Tew on tew@liverpool.ac.uk. To apply for this opportunity, please send your CV and covering letter to Dr Simon Tew.

Availability

Open to students worldwide

Funding information

Self-funded project

The successful candidate should have, or expect to have an Honours Degree at 2.1 or above (or equivalent) in a biological sciences related subject. Candidates whose first language is not English should have an IELTS score of 6.5 or equivalent.

This project is unfunded. The successful applicant will be expected to provide the funding for tuition fees (see https://www.liverpool.ac.uk/study/postgraduate-research/fees-and-funding/fees-and-costs/ ) and living expenses, as well as research costs of £12,000 per annum.

Supervisors