The role of lysosomes during mitosis

Description

Biology pulls off many apparently magical feats. None perhaps is as amazing as the transformation of a single fertilised egg into an adult human comprising trillions of individual cells. This cellular expansion is mainly driven by the process of mitosis, when a cell copies itself to make two new cells. Mitosis therefore underpins normal growth, development and ageing. Understanding how mitosis is regulated will reveal important insights into these processes and many human diseases. This project will combine cell/molecular-biology with high-resolution fluorescence imaging of live/fixed cells and proteomics/data science to understand how lysosomes function during mitosis in human cells. The project will examine how lysosomal calcium, phosphoinositides and protein phosphorylation impact on the way cells divide. A function for lysosomes in mitosis has yet to be investigated and this project aims to reveal a new feature of cell division which could, in future, lead to new ways of controlling proliferation. 

If you are interested in applying then please contact Dr Lee Haynes on 

Availability

Open to students worldwide

Funding information

Funded studentship

This is a self-funded project. University of Liverpool tuition fees and laboratory bench fees of ~£13,000/year for each of the three years of the project are required.

Supervisors

References

1: Helassa N, et al. A centrosome-localized calcium signal is essential for mammalian cell mitosis. FASEB J. 2019 Dec;33(12):14602-14610. doi: 10.1096/fj.201901662R.
2: Rajamanoharan D, et al. Modulation of phosphatidylinositol 4-phosphate levels by CaBP7 controls cytokinesis in mammalian cells. Mol Biol Cell. 2015 Apr 15;26(8):1428-39. doi: 10.1091/mbc.E14-07-1243.