An important part of maintaining reproducible and reliable chromatography, and to maintain a good spray at the emitter, is a high level of care in buffer preparation. Ways to compromise the chromatography include: NOT following these instructions carefully, using bottles other than those set aside for the nanoAcquity systems, using anything other than ultrapure reagents and ignoring the changeover frequency
We have decided to change all nanoAquity buffers weekly. This is intended to be a shared responsibility, and there must be no sense of deliberately avoiding Monday morning sessions to avoid the buffer change. In fact, you could argue that being the first person to use the system after a buffer change ensures the highest chance of success.
Lastly, it is essential that the buffer changes be logged, in a single forum entry per instrument, with a one line update
We have decided to change all nanoAquity buffers weekly. This is intended to be a shared responsibility, and there must be no sense of deliberately avoiding Monday morning sessions to avoid the buffer change. In fact, you could argue that being the first person to use the system after a buffer change ensures the highest chance of success.
Lastly, it is essential that the buffer changes be logged, in a single forum entry per instrument, with a one line update
Step 1: Solutions
Buffer A
HPLC water, 0.1% formic acid
200 ml HPLC grade water
Add 200 µl formic acid
(prepare in cylinder, mix on transfer to Duran)
Buffer B (also ‘strong wash’)
HPLC acetonitrile, 0.1% formic acid
200 ml HPLC grade acetonitrile
Add 200 µl formic acid
Weak wash
0.1% trifluoroacetic acid
500 ml HPLC grade water
Add 0.5 ml trifluoroacetic acid
Seal wash
10% acetonitrile
450ml HPLC grade water
Add 50 ml acetonitrile
[MDA formic acid]
[MDA trifluoroacetic acid]
[MDA acetonitrile]
[PDF version]
Created: Amy Claydon, 16/04/2011
Revisions: Amy, 21/04/2011 (changes to volumes, and to procedures)