Another new paper on glycan characterisation from Eyers team
Filed in: Papers
In an exciting advance to our earlier work using ion mobility-mass spectrometry for glycan characterisation (http://www.nature.com/nchem/journal/v6/n1/abs/nchem.1817.html), Claire Eyers and colleagues have published an article in Analytical Chemistry on the application of IM-MS and IR spectroscopy for 'Bottom up elucidation of glycosidic bond stereochemistry'
http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b04998?journalCode=ancham
This study demonstrated that information about the nature (sterio- and regio- chemistry) of glycans is retained during tandem mass spectrometry, which allows their composition, connectivity and configuration to be determined using ion mobility MS from small amounts of biological material. This is a significant advance as it enabled the structure of biologically relevant plant glycosides to be defined which was previously inaccessible by other analytical techniques, including NMR. These studies thus pave the way for a high-throughput method for glycan sequencing, which is currently not possible.
http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b04998?journalCode=ancham
This study demonstrated that information about the nature (sterio- and regio- chemistry) of glycans is retained during tandem mass spectrometry, which allows their composition, connectivity and configuration to be determined using ion mobility MS from small amounts of biological material. This is a significant advance as it enabled the structure of biologically relevant plant glycosides to be defined which was previously inaccessible by other analytical techniques, including NMR. These studies thus pave the way for a high-throughput method for glycan sequencing, which is currently not possible.