Research Assistant
Protein Function Group
Institute of Integrative Biology
University of Liverpool,
Crown Street
Liverpool L69 7ZB
Tel: +44 151 795 4392
Email: vharman[at]liv.ac.uk
About me
I completed my undergraduate degree in Biomedical Sciences at the University of Southampton. After graduating I moved to Liverpool to take a Research Assistant post in PFG. My role was funded by the BBSRC as part of the LOLA CoPYCAT project; a large-scale project, across two sites, in Manchester and Liverpool. The project aims to provide quantification data, to the level of molecules-per-cell, for the Saccharomyces cerevisiae proteome. The absolute quantification strategy utilises an internal, stable isotope labelled, QconCAT protein standard. A large part of my role was to express the QconCAT proteins, through E.coli culture, and prepare them for use in the quantification workflow; I was also involved in acquiring and processing the quantification data. During my time with the PFG, in addition to my Research Assistant role, I have also completed an MPhil in my spare time, supervised by Prof Beynon. In my thesis, entitled “QconCAT method development and applications in proteomics”, I reviewed how I shortened and streamlined the production workflow for the QconCAT protein standards. My current work is with Prof Beynon’s fellowship and involves collaborative work with Avacta and their Affimer technology.
Publications
I completed my undergraduate degree in Biomedical Sciences at the University of Southampton. After graduating I moved to Liverpool to take a Research Assistant post in PFG. My role was funded by the BBSRC as part of the LOLA CoPYCAT project; a large-scale project, across two sites, in Manchester and Liverpool. The project aims to provide quantification data, to the level of molecules-per-cell, for the Saccharomyces cerevisiae proteome. The absolute quantification strategy utilises an internal, stable isotope labelled, QconCAT protein standard. A large part of my role was to express the QconCAT proteins, through E.coli culture, and prepare them for use in the quantification workflow; I was also involved in acquiring and processing the quantification data. During my time with the PFG, in addition to my Research Assistant role, I have also completed an MPhil in my spare time, supervised by Prof Beynon. In my thesis, entitled “QconCAT method development and applications in proteomics”, I reviewed how I shortened and streamlined the production workflow for the QconCAT protein standards. My current work is with Prof Beynon’s fellowship and involves collaborative work with Avacta and their Affimer technology.
Publications
- Philip Brownridge, Stephen W Holman, Simon J Gaskell, Christopher M Grant, Victoria M Harman, Simon J Hubbard, Karin Lanthaler, Craig Lawless, Ronan O’cualain, Paul Sims, Rachel Watkins and Robert J Beynon (2011) ‘Global absolute quantification of a proteome: Challenges in the deployment of a QconCAT strategy’ Proteomics, 11(15), 2957-2970.
- Philip Brownridge, Victoria Harman, Deborah M Simpson, and Robert J Beynon (2011) ‘Absolute Multiplexed Protein Quantification Using QconCAT technology’ Methods in Molecular Biology - Quantitative Methods in Proteomics, 893, 267-293.
- Stine L. Bislev, Ulrike Kusebauch, Marius C. Codrea, Robert J. Beynon, Victoria M. Harman, Christine M. Røntved, Ruedi Aebersold, Robert L. Moritz and Emøke Bendixen (2011) ‘Quantotypic properties of QconCAT peptides targeting bovine host response to Streptococcus uberis." J Proteome Res, 11(3), 1832-1843.
- Victoria Harman (November 2011) ‘Meeting report, 8th BSPR-EBI meeting: from the visible to the hidden proteome’ Mass Matters the official publication of the British Mass Spectrometry Society, Edition 66, 16-17.
- Da Qi, Philip Brownridge, Dong Xia, Katherine Mackay, Faviel F. Gonzalez-Galarza, Jenna Kenyani, Victoria Harman, Robert J. Beynon, and Andrew R. Jones (2012) “A Software Toolkit and Interface for Performing Stable Isotope Labeling and Top3 Quantification Using Progenesis LC-MS” OMICS A Journal of Integrative Biology, 16(9), 489–495
- Philip Brownridge, Craig Lawless, Aishwarya B. Payapilly, Karin Lanthaler, Stephen W. Holman, Victoria M. Harman, Christopher M. Grant, Robert J. Beynon, Simon J. Hubbard (2013) “Quantitative analysis of chaperone network throughput in budding yeast” Proteomics, Volume 13, Issue 8, 1276–1291.