2023
Ventura, I., Harman, V., Beynon, R. J., & Santos, R. (2023). Glycoproteins Involved in Sea Urchin Temporary Adhesion. MARINE DRUGS, 21(3). doi:10.3390/md21030145DOI: 10.3390/md21030145
Rusilowicz, M., Newman, D. W., Creamer, D. R., Johnson, J., Adair, K., Harman, V. M., . . . Hubbard, S. J. (2023). AlacatDesigner-Computational Design of Peptide Concatamers for Protein Quantitation. JOURNAL OF PROTEOME RESEARCH, 22(2), 594-604. doi:10.1021/acs.jproteome.2c00608DOI: 10.1021/acs.jproteome.2c00608
Neves, L. X., Wilson, R. A., Brownridge, P., Holman, S. W. W., Harman, V. M. M., Eyers, C. E. E., . . . Castro-Borges, W. (2023). Dissection of schistosome tissues under LC-MS compatible preservative conditions for quantitative proteomics. RAPID COMMUNICATIONS IN MASS SPECTROMETRY. doi:10.1002/rcm.9523DOI: 10.1002/rcm.9523
2022
Maher, R. E., Barrett, E., Beynon, R. J., Harman, V. M., Jones, A. M., McNamara, P. S., . . . Lord, R. W. (2022). The relationship between lung disease severity and the sputum proteome in cystic fibrosis. RESPIRATORY MEDICINE, 204. doi:10.1016/j.rmed.2022.107002DOI: 10.1016/j.rmed.2022.107002
Sun, Y., Harman, V. M., Johnson, J. R., Brownridge, P. J., Chen, T., Dykes, G. F., . . . Liu, L. -N. (2022). Decoding the Absolute Stoichiometric Composition and Structural Plasticity of α-Carboxysomes. MBIO, 13(2). doi:10.1128/mbio.03629-21DOI: 10.1128/mbio.03629-21
Elsemman, I. E., Rodriguez Prado, A., Grigaitis, P., Garcia Albornoz, M., Harman, V., Holman, S. W., . . . Teusink, B. (2022). Whole-cell modeling in yeast predicts compartment-specific proteome constraints that drive metabolic strategies.. Nature communications, 13(1), 801. doi:10.1038/s41467-022-28467-6DOI: 10.1038/s41467-022-28467-6
2021
Sun, Y., Harman, V., Johnson, J., Chen, T., Dykes, G., Lin, Y., . . . Liu, L. -N. (2021). Decoding the absolute stoichiometric composition and structural plasticity of α-carboxysomes. doi:10.1101/2021.12.06.471529DOI: 10.1101/2021.12.06.471529
Johnson, J., Harman, V. M., Franco, C., Emmott, E., Rockliffe, N., Sun, Y., . . . Beynon, R. J. (2021). Construction of a la carte QconCAT protein standards for multiplexed quantification of user-specified target proteins. BMC BIOLOGY, 19(1). doi:10.1186/s12915-021-01135-9DOI: 10.1186/s12915-021-01135-9
Whole-cell modeling in yeast predicts compartment-specific proteome constraints that drive metabolic strategies (Journal article)
Elsemman, I. E., Prado, A. R., Grigaitis, P., Albornoz, M. G., Harman, V., Holman, S. W., . . . Teusink, B. (2021). Whole-cell modeling in yeast predicts compartment-specific proteome constraints that drive metabolic strategies. doi:10.1101/2021.06.11.448029DOI: 10.1101/2021.06.11.448029
2020
Takemori, A., Butcher, D. S., Harman, V. M., Brownridge, P., Shima, K., Higo, D., . . . Takemori, N. (2020). PEPPI-MS: Polyacrylamide-Gel-Based Prefractionation for Analysis of Intact Proteoforms and Protein Complexes by Mass Spectrometry. JOURNAL OF PROTEOME RESEARCH, 19(9), 3779-3791. doi:10.1021/acs.jproteome.0c00303DOI: 10.1021/acs.jproteome.0c00303
P168 The sputum proteome in cystic fibrosis lung disease and healthy volunteers (Journal article)
Lord, R. W., Maher, R. E., Harman, V. M., McNamara, P. S., Smith, J. A., Beynon, R. J., & Jones, A. M. (2020). P168 The sputum proteome in cystic fibrosis lung disease and healthy volunteers. Journal of Cystic Fibrosis, 19, S103. doi:10.1016/s1569-1993(20)30503-8DOI: 10.1016/s1569-1993(20)30503-8
Yang, M., Simpson, D., Wenner, N., Brownridge, P., Harman, V., Hinton, J., . . . Liu, L. (2020). Decoding the stoichiometric composition and organisation of bacterial metabolosomes. Nature Communications, 11(1). doi:10.1038/s41467-020-15888-4DOI: 10.1038/s41467-020-15888-4
Neves, L. X., Wilson, R. A., Brownridge, P., Harman, V. M., Holman, S. W., Beynon, R. J., . . . Castro-Borges, W. (2020). Quantitative Proteomics of Enriched Esophageal and Gut Tissues from the Human Blood Fluke <i>Schistosoma mansoni</i> Pinpoints Secreted Proteins for Vaccine Development. JOURNAL OF PROTEOME RESEARCH, 19(1), 314-326. doi:10.1021/acs.jproteome.9b00531DOI: 10.1021/acs.jproteome.9b00531
2019
Vileigas, D. F., Harman, V. M., Freire, P. P., Marciano, C. L. C., Sant'Ana, P. G., de Souza, S. L. B., . . . Cicogna, A. C. (2019). Landscape of heart proteome changes in a diet-induced obesity model. SCIENTIFIC REPORTS, 9. doi:10.1038/s41598-019-54522-2DOI: 10.1038/s41598-019-54522-2
THE SPUTUM PROTEOME AND ITS RELATIONSHIP TO CYSTIC FIBROSIS LUNG DISEASE: USING GLOBAL PROTEOMICS TO DEVELOP CLINICALLY USEFUL BIOMARKERS (Conference Paper)
Lord, R. W., Maher, R. E., Harman, V., Bianco, B., Whorwell, P. J., McNamara, P. S., . . . Jones, A. M. (2019). THE SPUTUM PROTEOME AND ITS RELATIONSHIP TO CYSTIC FIBROSIS LUNG DISEASE: USING GLOBAL PROTEOMICS TO DEVELOP CLINICALLY USEFUL BIOMARKERS. In THORAX Vol. 74 (pp. A12-A13). doi:10.1136/thorax-2019-BTSabstracts2019.24DOI: 10.1136/thorax-2019-BTSabstracts2019.24
Sarsby, J., McLean, L., Harman, V. M., & Beynon, R. J. (n.d.). Monitoring recombinant protein expression in bacteria by rapid evaporative ionisation mass spectrometry.. Rapid communications in mass spectrometry : RCM, e8670. doi:10.1002/rcm.8670DOI: 10.1002/rcm.8670
PEPPI-MS: Strategies to Enhance the Extraction of Electrophoretically Separated Proteins from Polyacrylamide Gels and Their Application to Top-Down/native Mass Spectrometry (Preprint)
DOI: 10.26434/chemrxiv.9917225
PEPPI-MS: Strategies to Enhance the Extraction of Electrophoretically Separated Proteins from Polyacrylamide Gels and Their Application to Top-Down/native Mass Spectrometry (Preprint)
DOI: 10.26434/chemrxiv.9917225.v1
Using sputum proteomics to develop a test for reflux aspiration in cystic fibrosis patients (Conference Paper)
Lord, R., Maher, R., Harman, V., Bianco, B., Mcnamara, P., Beynon, R., . . . Jones, A. (2019). Using sputum proteomics to develop a test for reflux aspiration in cystic fibrosis patients. In EUROPEAN RESPIRATORY JOURNAL Vol. 54. doi:10.1183/13993003.congress-2019.PA974DOI: 10.1183/13993003.congress-2019.PA974
P199 Does increased gastro-oesophageal reflux in cystic fibrosis patients alter the sputum proteome? (Journal article)
Maher, R. E., Lord, R. W., Harman, V. M., Smith, J. A., Jones, A. M., McNamara, P., & Beynon, R. J. (2019). P199 Does increased gastro-oesophageal reflux in cystic fibrosis patients alter the sputum proteome?. Journal of Cystic Fibrosis, 18, S113-S114. doi:10.1016/s1569-1993(19)30493-xDOI: 10.1016/s1569-1993(19)30493-x
2018
THE USE OF QUANTITATIVE PROTEOMICS TO ASSESS THE IMPACT OF REFLUX ASPIRATION ON CYSTIC FIBROSIS LUNG DISEASE (Conference Paper)
Lord, R. W., Maher, R. E., Harman, V. M., Treadway, S., Bianco, B., Barry, P. J., . . . Jones, A. M. (2018). THE USE OF QUANTITATIVE PROTEOMICS TO ASSESS THE IMPACT OF REFLUX ASPIRATION ON CYSTIC FIBROSIS LUNG DISEASE. In THORAX Vol. 73 (pp. A162). doi:10.1136/thorax-2018-212555.270DOI: 10.1136/thorax-2018-212555.270
2017
Takemori, N., Takemori, A., Tanaka, Y., Endo, Y., Hurst, J. L., Gomez-Baena, G., . . . Beynon, R. J. (2017). MEERCAT: Multiplexed Efficient Cell Free Expression of Recombinant QconCATs For Large Scale Absolute Proteome Quantification. Molecular and Cellular Proteomics, 16(12), 2169-2183. doi:10.1074/mcp.RA117.000284DOI: 10.1074/mcp.RA117.000284
Multiple QconCAT biosynthesis in a cell-free protein synthesis system (Conference Paper)
Takemori, N., Takemori, A., Tanaka, Y., Endo, Y., Hurst, J. L., Gomez-Baena, G., . . . Beynon, R. J. (2017). Multiple QconCAT biosynthesis in a cell-free protein synthesis system. In MOLECULAR & CELLULAR PROTEOMICS Vol. 16 (pp. S31). Retrieved from https://www.webofscience.com/
2016
Quantitative proteomics: it's all about training, of course! (Journal article)
Beynon, R., Harman, V., & Russell, H. (2016). Quantitative proteomics: it's all about training, of course!. The Biochemist, 38(4), 58-59. doi:10.1042/bio03804058DOI: 10.1042/bio03804058
A selected reaction monitoring-based analysis of acute phase proteins in interstitial fluids from experimental equine wounds healing by secondary intention (Journal article)
Bundgaard, L., Bendixen, E., Sorensen, M. A., Harman, V. M., Beynon, R. J., Petersen, L. J., & Jacobsen, S. (2016). A selected reaction monitoring-based analysis of acute phase proteins in interstitial fluids from experimental equine wounds healing by secondary intention. WOUND REPAIR AND REGENERATION, 24(3), 525-532. doi:10.1111/wrr.12425DOI: 10.1111/wrr.12425
Lawless, C., Holman, S. W., Brownridge, P., Lanthaler, K., Harman, V. M., Watkins, R., . . . Hubbard, S. J. (2016). Direct and Absolute Quantification of over 1800 Yeast Proteins via Selected Reaction Monitoring. Molecular and Cellular Proteomics, 15(4), 1309-1322. doi:10.1074/mcp.M115.054288DOI: 10.1074/mcp.M115.054288
2015
Mass spectrometry for structural analysis and quantification of the Major Urinary Proteins of the house mouse (Journal article)
Beynon, R. J., Armstrong, S. D., Claydon, A. J., Davidson, A. J., Eyers, C. E., Langridge, J. I., . . . Woolerton, Y. E. (2015). Mass spectrometry for structural analysis and quantification of the Major Urinary Proteins of the house mouse. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 391, 146-156. doi:10.1016/j.ijms.2015.07.026DOI: 10.1016/j.ijms.2015.07.026
2014
Development of a Method for Absolute Quantification of Equine Acute Phase Proteins Using Concatenated Peptide Standards and Selected Reaction Monitoring (Journal article)
Bundgaard, L., Jacobsen, S., Dyrlund, T. F., Sorensen, M. A., Harman, V. M., Beynon, R. J., . . . Bendixen, E. (2014). Development of a Method for Absolute Quantification of Equine Acute Phase Proteins Using Concatenated Peptide Standards and Selected Reaction Monitoring. JOURNAL OF PROTEOME RESEARCH, 13(12), 5635-5647. doi:10.1021/pr500607sDOI: 10.1021/pr500607s
The role of proteomics in studies of protein moonlighting (Journal article)
Beynon, R. J., Hammond, D., Harman, V., & Woolerton, Y. (2014). The role of proteomics in studies of protein moonlighting. BIOCHEMICAL SOCIETY TRANSACTIONS, 42, 1698-1703. doi:10.1042/BST20140277DOI: 10.1042/BST20140277
2013
Quantitative analysis of chaperone network throughput in budding yeast (Journal article)
Brownridge, P., Lawless, C., Payapilly, A. B., Lanthaler, K., Holman, S. W., Harman, V. M., . . . Hubbard, S. J. (2013). Quantitative analysis of chaperone network throughput in budding yeast. PROTEOMICS, 13(8), 1276-1291. doi:10.1002/pmic.201200412DOI: 10.1002/pmic.201200412
CoPY - Census of the Proteome of Yeast: Quantification of the <i>S</i>. <i>cerevisiae</i> proteome (Conference Paper)
Lanthaler, K., Holman, S., Lawless, C., Watkins, R., Brownridge, P., Harman, V., . . . Beynon, R. (2013). CoPY - Census of the Proteome of Yeast: Quantification of the <i>S</i>. <i>cerevisiae</i> proteome. In YEAST Vol. 30 (pp. 188). Retrieved from https://www.webofscience.com/
2012
A Software Toolkit and Interface for Performing Stable Isotope Labeling and Top3 Quantification Using Progenesis LC-MS (Journal article)
Qi, D., Brownridge, P., Xia, D., Mackay, K., Gonzalez-Galarza, F. F., Kenyani, J., . . . Jones, A. R. (2012). A Software Toolkit and Interface for Performing Stable Isotope Labeling and Top3 Quantification Using Progenesis LC-MS. OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY, 16(9), 489-495. doi:10.1089/omi.2012.0042DOI: 10.1089/omi.2012.0042
Absolute multiplexed protein quantification using QconCAT technology. (Journal article)
Brownridge, P. J., Harman, V. M., Simpson, D. M., & Beynon, R. J. (2012). Absolute multiplexed protein quantification using QconCAT technology.. Methods in molecular biology (Clifton, N.J.), 893, 267-293. doi:10.1007/978-1-61779-885-6_18DOI: 10.1007/978-1-61779-885-6_18
Quantotypic Properties of QconCAT Peptides Targeting Bovine Host Response to <i>Streptococcus uberis</i> (Journal article)
Bislev, S. L., Kusebauch, U., Codrea, M. C., Beynon, R. J., Harman, V. M., Rontved, C. M., . . . Bendixen, E. (2012). Quantotypic Properties of QconCAT Peptides Targeting Bovine Host Response to <i>Streptococcus uberis</i>. JOURNAL OF PROTEOME RESEARCH, 11(3), 1832-1843. doi:10.1021/pr201064gDOI: 10.1021/pr201064g
2011
Global absolute quantification of a proteome: Challenges in the deployment of a QconCAT strategy (Journal article)
Brownridge, P., Holman, S. W., Gaskell, S. J., Grant, C. M., Harman, V. M., Hubbard, S. J., . . . Beynon, R. J. (2011). Global absolute quantification of a proteome: Challenges in the deployment of a QconCAT strategy. PROTEOMICS, 11(15), 2957-2970. doi:10.1002/pmic.201100039DOI: 10.1002/pmic.201100039
Absolute Multiplexed Protein Quantification Using QconCAT technology (Chapter)
Brownridge, P. J., Harman, V., Simpson, D. M., & Beynon, R. J. (2011). Absolute Multiplexed Protein Quantification Using QconCAT technology. In K. Marcus (Ed.), Quantitative Methods in Proteomics (pp. 100-115). New York: Humana Press.
Alternative routes to absolute protein quantification (Conference Paper)
Brownridge, P. J., Harman, V. M., Lawless, C., Lanthaler, K., Watkins, R., Holman, S. W., . . . Beynon, R. J. (2011). Alternative routes to absolute protein quantification. In 8th BSPR-EBI Meeting 'From the visible to the hidden proteome' (pp. Poster). Cambridge, UK: BSPR-EBI Meeting.
Large scale production of QconCAT standards for quantitative proteomics (Conference Paper)
Harman, V., Watkins, R. H., Brownridge, P., Holman, S. W., Lanthaler, K., Lawless, C., . . . Beynon, R. (2011). Large scale production of QconCAT standards for quantitative proteomics. In 8th BSPR-EBI Meeting 'From the visible to the hidden proteome' (pp. Poster). Cambridge, UK: BSPR-EBI Meeting.
Undated
Harman, V. (n.d.). QconCAT method development and applications in proteomics. (Master's Thesis, University of Liverpool).
