Photo of Professor Bob Eady

Professor Bob Eady BSc, PhD, DSc

Senior Research Fellow Biochemistry & Systems Biology

    Publications

    2016

    Serial crystallography captures enzyme catalysis in copper nitrite reductase at atomic resolution from one crystal (Journal article)

    Horrell, S., Antonyuk, S. V., Eady, R. R., Hasnain, S. S., Hough, M. A., & Strange, R. W. (2016). Serial crystallography captures enzyme catalysis in copper nitrite reductase at atomic resolution from one crystal. IUCrJ, 3(Part 4), 271-281. doi:10.1107/S205225251600823X

    DOI: 10.1107/S205225251600823X

    Fresh insight to functioning of selected enzymes of the nitrogen cycle (Journal article)

    Eady, R. R., Antonyuk, S. V., & Hasnain, S. S. (2016). Fresh insight to functioning of selected enzymes of the nitrogen cycle. CURRENT OPINION IN CHEMICAL BIOLOGY, 31, 103-112. doi:10.1016/j.cbpa.2016.02.009

    DOI: 10.1016/j.cbpa.2016.02.009

    2014

    Impact of residues remote from the catalytic centre on enzyme catalysis of copper nitrite reductase (Journal article)

    Leferink, N. G. H., Antonyuk, S., Houwman, J. A., Scrutton, N., Eady, R. R., & Hasnain, S. S. (2014). Impact of residues remote from the catalytic centre on enzyme catalysis of copper nitrite reductase. Nature Communications, 5. doi:10.1038/ncomms5395

    DOI: 10.1038/ncomms5395

    Fingerprinting redox and ligand states in haemprotein crystal structures using resonance Raman spectroscopy (Journal article)

    Kekilli, D., Dworkowski, F. S. N., Pompidor, G., Fuchs, M. R., Andrew, C. R., Antonyuk, S., . . . Hough, M. A. (2014). Fingerprinting redox and ligand states in haemprotein crystal structures using resonance Raman spectroscopy. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 70, 1289-1296. doi:10.1107/S1399004714004039

    DOI: 10.1107/S1399004714004039

    2013

    Structures of protein-protein complexes involved in electron transfer (Journal article)

    Antonyuk, S. V., Han, C., Eady, R. R., & Hasnain, S. S. (2013). Structures of protein-protein complexes involved in electron transfer. NATURE, 496(7443), 123-127. doi:10.1038/nature11996

    DOI: 10.1038/nature11996

    2012

    Characterization of a novel copper-haem c dissimilatory nitrite reductase from Ralstonia pickettii (Journal article)

    Han, C., Wright, G. S. A., Fisher, K., Rigby, S. E. J., Eady, R. R., & Hasnain, S. S. (2012). Characterization of a novel copper-haem c dissimilatory nitrite reductase from Ralstonia pickettii. BIOCHEMICAL JOURNAL, 444, 219-226. doi:10.1042/BJ20111623

    DOI: 10.1042/BJ20111623

    Laser-flash photolysis indicates that internal electron transfer is triggered by proton uptake by Alcaligenes xylosoxidans copper-dependent nitrite reductase (Journal article)

    Leferink, N. G. H., Eady, R. R., Hasnain, S. S., & Scrutton, N. S. (2012). Laser-flash photolysis indicates that internal electron transfer is triggered by proton uptake by Alcaligenes xylosoxidans copper-dependent nitrite reductase. FEBS Journal, 279(12), 2174-2181. doi:10.1111/j.1742-4658.2012.08601.x

    DOI: 10.1111/j.1742-4658.2012.08601.x

    A Distal Pocket Leu Residue Inhibits the Binding of O2 and NO at the Distal Heme Site of Cytochrome c' (Journal article)

    Garton, E. M., Pixton, D. A., Petersen, C. A., Eady, R. R., Hasnain, S. S., & Andrew, C. R. (2012). A Distal Pocket Leu Residue Inhibits the Binding of O2 and NO at the Distal Heme Site of Cytochrome c'. Journal of the American Chemical Society, 134(3), 1461-1463. doi:10.1021/ja209770p

    DOI: 10.1021/ja209770p

    Characterization of a novel copperheme c dissimilatory nitrite reductase from Ralstonia pickettii (Journal article)

    Han, C., Wright, G. S. A., Fisher, K., Rigby, S. E. J., Eady, R. R., & Hasnain, S. S. (2012). Characterization of a novel copperheme c dissimilatory nitrite reductase from Ralstonia pickettii. Biochemical Journal, 444, 216-219.

    2011

    Carbon monoxide poisoning is prevented by the energy costs of conformational changes in gas-binding haemproteins (Journal article)

    Antonyuk, S. V., Rustage, N., Petersen, C. A., Arnst, J. L., Heyes, D. J., Sharma, R., . . . Hasnain, S. S. (2011). Carbon monoxide poisoning is prevented by the energy costs of conformational changes in gas-binding haemproteins. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 108(38), 15780-15785. doi:10.1073/pnas.1109051108

    DOI: 10.1073/pnas.1109051108

    Distal-to-Proximal NO Conversion in Hemoproteins: The Role of the Proximal Pocket (Journal article)

    Hough, M. A., Antonyuk, S. V., Barbieri, S., Rustage, N., McKay, A. L., Servid, A. E., . . . Hasnain, S. S. (2011). Distal-to-Proximal NO Conversion in Hemoproteins: The Role of the Proximal Pocket. JOURNAL OF MOLECULAR BIOLOGY, 405(2), 395-409. doi:10.1016/j.jmb.2010.10.035

    DOI: 10.1016/j.jmb.2010.10.035

    Electron Transfer and Half Reactivity in Nitrogenase. (Journal article)

    Clark, T. A., Fairhurst, S., Lowe, D. J., Watmough, N. J., & Eady, R. R. (2011). Electron Transfer and Half Reactivity in Nitrogenase.. Biochemical Society Transactions, 39, 201-206.

    Gating Mechanisms for Biological Electron Transfer: Integrating Structure with Biophysics Reveals the Nature of Redox Control in Cytochrome P450 reductase and Copper-Dependent Nitrite Reductase. (Journal article)

    Leferink, N. G. H., Pudney, C. R., Brenner, S., Heys, D. J., Eady, R. R., Hasnain, S. S., . . . Scrutton, N. S. (2011). Gating Mechanisms for Biological Electron Transfer: Integrating Structure with Biophysics Reveals the Nature of Redox Control in Cytochrome P450 reductase and Copper-Dependent Nitrite Reductase.. FEBS Letters, 586, 578-584.

    Mechanism of Proton-Coupled Electron Transfer in the Catalytic Cycle of Alcaligenes xylosoxidans Copper-Dependent Nitrite Reductase (Journal article)

    Leferink, N. G. H., Han, C., Antonyuk, S. V., Heyes, D. J., Rigby, S. E. J., Hough, M. A., . . . Hasnain, S. S. (2011). Mechanism of Proton-Coupled Electron Transfer in the Catalytic Cycle of Alcaligenes xylosoxidans Copper-Dependent Nitrite Reductase. Biochemistry, 50, 4121-4131. doi:10.1021/bi

    DOI: 10.1021/bi

    2010

    Characterization of cycP gene expression in Achromobacter xylosoxidans NCIMB 11015 and high-level heterologous synthesis of cytochrome c' in Escherichia coli. (Journal article)

    Harris, R. L., Barbieri, S., Paraskevopoulos, K., Murphy, L. M., Eady, R. R., Hasnain, S. S., & Sawers, R. G. (2010). Characterization of cycP gene expression in Achromobacter xylosoxidans NCIMB 11015 and high-level heterologous synthesis of cytochrome c' in Escherichia coli.. Journal of molecular microbiology and biotechnology, 18(2), 102-108. doi:10.1159/000287989

    DOI: 10.1159/000287989

    2009

    Demonstration of Proton-coupled Electron Transfer in the Copper-containing Nitrite Reductases (Journal article)

    Brenner, S., Heyes, D. J., Hay, S., Hough, M. A., Eady, R. R., Hasnain, S. S., & Scrutton, N. S. (2009). Demonstration of Proton-coupled Electron Transfer in the Copper-containing Nitrite Reductases. Journal of Biological Chemistry, 284(38), 25973-25983. doi:10.1074/jbc.m109.012245

    DOI: 10.1074/jbc.m109.012245

    2008

    Identification of the Proton Channel to the Active Site Type 2 Cu Center of Nitrite Reductase: Structural and Enzymatic Properties of the His254Phe and Asn90Ser Mutants†,‡ (Journal article)

    Hough, M. A., Eady, R. R., & Hasnain, S. S. (2008). Identification of the Proton Channel to the Active Site Type 2 Cu Center of Nitrite Reductase: Structural and Enzymatic Properties of the His254Phe and Asn90Ser Mutants†,‡. Biochemistry, 47(51), 13547-13553. doi:10.1021/bi801369y

    DOI: 10.1021/bi801369y

    Crystallography with online optical and X-ray absorption spectroscopies demonstrates an ordered mechanism in copper nitrite reductase (Journal article)

    Hough, M. A., Antonyuk, S. V., Strange, R. W., Eady, R. R., & Hasnain, S. S. (2008). Crystallography with online optical and X-ray absorption spectroscopies demonstrates an ordered mechanism in copper nitrite reductase. JOURNAL OF MOLECULAR BIOLOGY, 378(2), 353-361. doi:10.1016/j.jmb.2008.01.097

    DOI: 10.1016/j.jmb.2008.01.097

    2007

    Nitrous oxide reductase (Chapter)

    Eady, R. R., Antonyuk, S. V., & Hasnain, S. S. (2007). Nitrous oxide reductase. In A. Messerschmidt (Ed.), Handbook of Metalloproteins (pp. 1). london: John Wiley & Sons.

    2006

    Insight into catalysis of nitrous oxide reductase from high-resolution structures of resting and inhibitor-bound enzyme from Achromobacter cycloclastes (Journal article)

    Paraskevopoulos, K., Antonyuk, S. V., Sawers, R. G., Eady, R. R., & Hasnain, S. S. (2006). Insight into catalysis of nitrous oxide reductase from high-resolution structures of resting and inhibitor-bound enzyme from Achromobacter cycloclastes. JOURNAL OF MOLECULAR BIOLOGY, 362(1), 55-65. doi:10.1016/j.jmb.2006.06.064

    DOI: 10.1016/j.jmb.2006.06.064

    2005

    Atomic resolution structures of resting-state, substrate- and product-complexed Cu-nitrite reductase provide insight into catalytic mechanism (Journal article)

    Antonyuk, S. V., Strange, R. W., Sawers, G., Eady, R. R., & Hasnain, S. S. (2005). Atomic resolution structures of resting-state, substrate- and product-complexed Cu-nitrite reductase provide insight into catalytic mechanism. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 102(34), 12041-12046. doi:10.1073/pnas.0504207102

    DOI: 10.1073/pnas.0504207102

    High resolution structural studies of mutants provide insights into catalysis and electron transfer processes in copper nitrite reductase (Journal article)

    Hough, M. A., Ellis, M. J., Antonyuk, S., Strange, R. W., Sawers, G., Eady, R. R., & Hasnain, S. S. (2005). High resolution structural studies of mutants provide insights into catalysis and electron transfer processes in copper nitrite reductase. JOURNAL OF MOLECULAR BIOLOGY, 350(2), 300-309. doi:10.1016/j.jmb.2005.04.006

    DOI: 10.1016/j.jmb.2005.04.006

    2004

    Insights into redox partner interactions and substrate binding in nitrite reductase from Alcaligenes xylosoxidans: Crystal structures of the TrP138His and His313Gln mutants (Journal article)

    Barrett, M. L., Harris, R. L., Antonyuk, S., Hough, M. A., Ellis, M. J., Sawers, G., . . . Hasnain, S. S. (2004). Insights into redox partner interactions and substrate binding in nitrite reductase from Alcaligenes xylosoxidans: Crystal structures of the TrP138His and His313Gln mutants. BIOCHEMISTRY, 43(51), 16311-16319. doi:10.1021/bi048682g

    DOI: 10.1021/bi048682g

    2003

    The structure of glyceraldehyde 3-phosphate dehydrogenase from Alcaligenes xylosoxidans at 1.7 angstrom resolution (Journal article)

    Antonyuk, S. V., Eady, R. R., Strange, R. W., & Hasnain, S. S. (2003). The structure of glyceraldehyde 3-phosphate dehydrogenase from Alcaligenes xylosoxidans at 1.7 angstrom resolution. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 59, 835-842. doi:10.1107/S0907444903041441

    DOI: 10.1107/S0907444903041441