2024
Omar, M. H., Byrne, D. P., Shrestha, S., Lakey, T. M., Lee, K. -S., Lauer, S. M., . . . Scott, J. D. (2024). Discovery of a Cushing’s syndrome protein kinase A mutant that biases signaling through type I AKAPs. Science Advances, 10(8). doi:10.1126/sciadv.adl1258DOI: 10.1126/sciadv.adl1258
2023
Daly, L. A., Byrne, D. P., Perkins, S., Brownridge, P. J., McDonnell, E., Jones, A. R., . . . Eyers, C. E. (2023). Custom Workflow for the Confident Identification of Sulfotyrosine-Containing Peptides and Their Discrimination from Phosphopeptides.. Journal of proteome research, 22(12), 3754-3772. doi:10.1021/acs.jproteome.3c00425DOI: 10.1021/acs.jproteome.3c00425
Venkat, A., Watterson, G., Byrne, D. P., O'Boyle, B., Shrestha, S., Gravel, N., . . . Kannan, N. (2023). Mechanistic and evolutionary insights into isoform-specific 'supercharging' in DCLK family kinases.. eLife, 12, RP87958. doi:10.7554/elife.87958DOI: 10.7554/elife.87958
Cao, M., Day, A. M., Galler, M., Latimer, H. R., Byrne, D. P., Foy, T. W., . . . Veal, E. A. (2023). A peroxiredoxin-P38 MAPK scaffold increases MAPK activity by MAP3K-independent mechanisms. MOLECULAR CELL, 83(17), 3140-+. doi:10.1016/j.molcel.2023.07.018DOI: 10.1016/j.molcel.2023.07.018
Omar, M. H., Kihiu, M., Byrne, D. P., Lee, K. -S., Lakey, T. M., Butcher, E., . . . Scott, J. D. (2023). Classification of Cushing's syndrome PKAc mutants based upon their ability to bind PKI. BIOCHEMICAL JOURNAL, 480(12), 875-890. doi:10.1042/BCJ20230183DOI: 10.1042/BCJ20230183
Balmanno, K., Kidger, A. M., Byrne, D. P., Sale, M. J., Nassman, N., Eyers, P. A., & Cook, S. J. (2023). ERK1/2 inhibitors act as monovalent degraders inducing ubiquitylation and proteasome-dependent turnover of ERK2, but not ERK1. BIOCHEMICAL JOURNAL, 480(9), 587-605. doi:10.1042/BCJ20220598DOI: 10.1042/BCJ20220598
Byrne, D. P., Shrestha, S., Daly, L. A., Marensi, V., Ramakrishnan, K., Eyers, C. E., . . . Eyers, P. A. (2023). Evolutionary and cellular analysis of the 'dark' pseudokinase PSKH2. BIOCHEMICAL JOURNAL, 480(2), 141-160. doi:10.1042/BCJ20220474DOI: 10.1042/BCJ20220474
2022
Foulkes, D. M., McLean, K., Sloniecka, M., Rustidge, S., Byrne, D. P., Haneef, A. S., . . . Kaye, S. B. (2022). Impact of fluoroquinolones and aminoglycosides on<i> P.</i><i> aeruginosa</i> virulence factor production and. BIOCHEMICAL JOURNAL, 479(24), 2511-2527. doi:10.1042/BCJ20220527DOI: 10.1042/BCJ20220527
Luis, A. S., Basle, A., Byrne, D. P., Wright, G. S. A., London, J. A., Jin, C., . . . Cartmell, A. (2022). Sulfated glycan recognition by carbohydrate sulfatases of the human gut microbiota. NATURE CHEMICAL BIOLOGY, 18(8), 841-+. doi:10.1038/s41589-022-01039-xDOI: 10.1038/s41589-022-01039-x
Omar, M. H., Byrne, D. P., Jones, K. N., Lakey, T. M., Collins, K. B., Lee, K. -S., . . . Scott, J. D. (2022). Mislocalization of protein kinase A drives pathology in Cushing?s syndrome. CELL REPORTS, 40(2). doi:10.1016/j.celrep.2022.111073DOI: 10.1016/j.celrep.2022.111073
Exploring the conformational landscape and stability of Aurora A using ion-mobility mass spectrometry and molecular modelling (Journal article)
Tomlinson, L. J., Batchelor, M., Sarsby, J., Byrne, D. P., Brownridge, P., Bayliss, R., . . . Eyers, C. E. (2022). Exploring the conformational landscape and stability of Aurora A using ion-mobility mass spectrometry and molecular modelling. Journal of the American Society for Mass Spectrometry. doi:10.1021/jasms.1c00271DOI: 10.1021/jasms.1c00271
Analysis of human Tribbles 2 (TRIB2) pseudokinase (Chapter)
Harris, J. A., Fairweather, E., Byrne, D. P., & Eyers, P. A. (2022). Analysis of human Tribbles 2 (TRIB2) pseudokinase. In PSEUDOKINASES (Vol. 667, pp. 79-99). doi:10.1016/bs.mie.2022.03.025DOI: 10.1016/bs.mie.2022.03.025
Byrne, D. P., Omar, M. H., Kennedy, E. J., Eyers, P. A., & Scott, J. D. (2022). Biochemical Analysis of AKAP-Anchored PKA Signaling Complexes.. Methods in molecular biology (Clifton, N.J.), 2483, 297-317. doi:10.1007/978-1-0716-2245-2_19DOI: 10.1007/978-1-0716-2245-2_19
2021
Luis, A. S., Jin, C., Pereira, G. V., Glowacki, R. W. P., Gugel, S. R., Singh, S., . . . Martens, E. C. (2021). A single sulfatase is required to access colonic mucin by a gut bacterium. NATURE, 598(7880), 332-+. doi:10.1038/s41586-021-03967-5DOI: 10.1038/s41586-021-03967-5
Meyer, B., Chiaravalli, J., Gellenoncourt, S., Brownridge, P., Bryne, D. P., Daly, L. A., . . . Emmott, E. (2021). Characterising proteolysis during SARS-CoV-2 infection identifies viral cleavage sites and cellular targets with therapeutic potential. NATURE COMMUNICATIONS, 12(1). doi:10.1038/s41467-021-25796-wDOI: 10.1038/s41467-021-25796-w
Huang, L. -C., Taujale, R., Gravel, N., Venkat, A., Yeung, W., Byrne, D. P., . . . Kannan, N. (2021). KinOrtho: a method for mapping human kinase orthologs across the tree of life and illuminating understudied kinases.. BMC bioinformatics, 22(1), 446. doi:10.1186/s12859-021-04358-3DOI: 10.1186/s12859-021-04358-3
Byrne, D. P., London, J. A., Eyers, P. A., Yates, E. A., & Cartmell, A. (2021). Mobility shift-based electrophoresis coupled with fluorescent detection enables real-time enzyme analysis of carbohydrate sulfatase activity. BIOCHEMICAL JOURNAL, 478(4), 735-748. doi:10.1042/BCJ20200952DOI: 10.1042/BCJ20200952
2020
Shrestha, S., Byrne, D. P., Harris, J. A., Kannan, N., & Eyers, P. A. (2020). Cataloguing the dead: breathing new life into pseudokinase research. FEBS JOURNAL, 287(19), 4150-4169. doi:10.1111/febs.15246DOI: 10.1111/febs.15246
Shrestha, S., Katiyar, S., Sanz-Rodriguez, C. E., Kemppinen, N. R., Kim, H. W., Kadirvelraj, R., . . . Kannan, N. (2020). A redox-active switch in fructosamine-3-kinases expands the regulatory repertoire of the protein kinase superfamily. Science Signaling, 13(639). doi:10.1126/scisignal.aax6313DOI: 10.1126/scisignal.aax6313
Byrne, D. P., Shrestha, S., Galler, M., Cao, M., Daly, L. A., Campbell, A. E., . . . Eyers, P. A. (2020). Aurora A regulation by reversible cysteine oxidation reveals evolutionarily conserved redox control of Ser/Thr protein kinase activity. Science Signaling, 13(639). doi:10.1126/scisignal.aax2713DOI: 10.1126/scisignal.aax2713
Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signalling networks using SILAC-based phosphoproteomics (Journal article)
Byrne, D. P., Clarke, C. J., Brownridge, P. J., Kalyuzhnyy, A., Perkins, S., Campbell, A., . . . Eyers, C. E. (2020). Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signalling networks using SILAC-based phosphoproteomics. The Biochemical journal, 477(13), 2451-2475. doi:10.1042/bcj20200309DOI: 10.1042/bcj20200309
Analysis of 1-and 3-Phosphohistidine (pHis) Protein Modification Using Model Enzymes Expressed in Bacteria (Journal article)
Coldron, A. K. M. C., Byrne, D. P., & Eyers, P. A. (2020). Analysis of 1-and 3-Phosphohistidine (pHis) Protein Modification Using Model Enzymes Expressed in Bacteria. HISTIDINE PHOSPHORYLATION, 2077, 63-81. doi:10.1007/978-1-4939-9884-5_5DOI: 10.1007/978-1-4939-9884-5_5
Tsuchiya, Y., Byrne, D. P., Burgess, S. G., Bormann, J., Bakovic, J., Huang, Y., . . . Gout, I. (2020). Covalent Aurora A regulation by the metabolic integrator coenzyme A. REDOX BIOLOGY, 28. doi:10.1016/j.redox.2019.101318DOI: 10.1016/j.redox.2019.101318
2019
Hardman, G., Perkins, S., Brownridge, P., Clarke, C., Byrne, D., Campbell, A., . . . Eyers, C. (2019). Strong anion exchange-mediated phosphoproteomics reveals extensive human non-canonical phosphorylation. The EMBO Journal, 38(21). doi:10.15252/embj.2018100847DOI: 10.15252/embj.2018100847
Kershaw, N. M., Byrne, D. P., Parsons, H., Berry, N. G., Fernig, D. G., Eyers, P. A., & Cosstick, R. (2019). Structure-based design of nucleoside-derived analogues as sulfotransferase inhibitors. RSC ADVANCES, 9(55), 32165-32173. doi:10.1039/c9ra07567dDOI: 10.1039/c9ra07567d
Walden, M., Tian, L., Ross, R. L., Sykora, U. M., Byrne, D. P., Hesketh, E. L., . . . Zeqiraj, E. (2019). Metabolic control of BRISC-SHMT2 assembly regulates immune signalling. NATURE, 570(7760), 194-+. doi:10.1038/s41586-019-1232-1DOI: 10.1038/s41586-019-1232-1
BH3-only proteins are dispensable for apoptosis induced by pharmacological inhibition of both MCL-1 and BCL-X-L (Journal article)
Greaves, G., Milani, M., Butterworth, M., Carter, R. J., Byrne, D. P., Eyers, P. A., . . . Varadarajan, S. (2019). BH3-only proteins are dispensable for apoptosis induced by pharmacological inhibition of both MCL-1 and BCL-X-L. CELL DEATH AND DIFFERENTIATION, 26(6), 1037-1047. doi:10.1038/s41418-018-0183-7DOI: 10.1038/s41418-018-0183-7
Mears, P. R., Hoekman, S., Rye, C. E., Bailey, F. P., Byrne, D. P., Eyers, P. A., & Thomas, E. J. (2019). Towards 20,20-difluorinated bryostatin: synthesis and biological evaluation of C17,C27-fragments. ORGANIC & BIOMOLECULAR CHEMISTRY, 17(6), 1487-1505. doi:10.1039/c8ob03152eDOI: 10.1039/c8ob03152e
Vonderach, M., Byrne, D. P., Barran, P. E., Eyers, P. A., & Eyers, C. E. (2019). DNA Binding and Phosphorylation Regulate the Core Structure of the NF-B p50 Transcription Factor. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 30(1), 128-138. doi:10.1007/s13361-018-1984-0DOI: 10.1007/s13361-018-1984-0
2018
Foulkes, D. M., Byrne, D. P., Yeung, W., Shrestha, S., Bailey, F. P., Ferries, S., . . . Eyers, P. A. (2018). Covalent inhibitors of EGFR family protein kinases induce degradation of human Tribbles 2 (TRIB2) pseudokinase in cancer cells. SCIENCE SIGNALING, 11(549), 14 pages. doi:10.1126/scisignal.aat7951DOI: 10.1126/scisignal.aat7951
Byrne, D. P., Li, Y., Ngamlert, P., Ramakrishnan, K., Eyers, C. E., Wells, C., . . . Eyers, P. A. (2018). New tools for evaluating protein tyrosine sulfation: tyrosylprotein sulfotransferases (TPSTs) are novel targets for RAF protein kinase inhibitors. BIOCHEMICAL JOURNAL, 475(15), 2435-2455. doi:10.1042/BCJ20180266DOI: 10.1042/BCJ20180266
Combes, G., Barysz, H., Garand, C., Braga, L. G., Alharbi, I., Thebault, P., . . . Elowe, S. (2018). Mps1 Phosphorylates Its N-Terminal Extension to Relieve Autoinhibition and Activate the Spindle Assembly Checkpoint. CURRENT BIOLOGY, 28(6), 872-883. doi:10.1016/j.cub.2018.02.002DOI: 10.1016/j.cub.2018.02.002
2017
Smith, F. D., Esseltine, J. L., Nygren, P. J., Veesler, D., Byrne, D. P., Vonderach, M., . . . Scott, J. D. (2017). Local protein kinase A action proceeds through intact holoenzymes. Science, 356(6344), 1288-1293. doi:10.1126/science.aaj1669DOI: 10.1126/science.aaj1669
Byrne, D. P., Foulkes, D. M., & Eyers, P. A. (2017). Pseudokinases: update on their functions and evaluation as new drug targets. FUTURE MEDICINAL CHEMISTRY, 9(2), 245-265. doi:10.4155/fmc-2016-0207DOI: 10.4155/fmc-2016-0207
Milani, M., Byrne, D. P., Greaves, G., Butterworth, M., Cohen, G. M., Eyers, P. A., & Varadarajan, S. (2018). DRP-1 is required for BH3 mimetic-mediated mitochondrial fragmentation and apoptosis. Cell Death and Disease, 8. doi:10.1038/cddis.2016.485DOI: 10.1038/cddis.2016.485
2016
Caron, D., Byrne, D. P., Thebault, P., Soulet, D., Landry, C. R., Eyers, P. A., & Elowe, S. (2016). Mitotic phosphotyrosine network analysis reveals that tyrosine phosphorylation regulates Polo-like kinase 1 (PLK1). SCIENCE SIGNALING, 9(458). doi:10.1126/scisignal.aah3525DOI: 10.1126/scisignal.aah3525
Skimming, D., Dastgheib, S., Baffi, T., Byrne, D., Ferries, S., Scott, S., . . . Kannan, N. (2016). KinView: A visual comparative sequence analysis tool for integrated kinome research. Molecular BioSystems, (12), 3651-3665. doi:10.1039/C6MB00466KDOI: 10.1039/C6MB00466K
Barone, G., Staples, C., Ganesh, A., Patterson, K., Byrne, D., Myers, K., . . . Collis, S. (2016). Human CDK18 promotes replication stress signaling and genome stability. Nucleic Acids Research, 44(18), 8772-8785. doi:10.1093/nar/gkw615DOI: 10.1093/nar/gkw615
Byrne, D. P., Vonderach, M., Ferries, S., Brownridge, P. J., Eyers, C. E., & Eyers, P. A. (2016). cAMP-dependent protein kinase (PKA) complexes probed by complementary differential scanning fluorimetry and ion mobility-mass spectrometry. BIOCHEMICAL JOURNAL, 473, 3159-3175. doi:10.1042/BCJ20160648DOI: 10.1042/BCJ20160648
Mohanty, S., Oruganty, K., Kwon, A., Byrne, D., Ferries, S., Ruan, Z., . . . Kannan, N. (2016). Hydrophobic Core Variations Provide a Structural Framework for Tyrosine Kinase Evolution and Functional Specialization.. PLoS Genetics, 12(2). doi:10.1371/journal.pgen.1005885DOI: 10.1371/journal.pgen.1005885
2015
Tribbles pseudokinases: novel targets for chemical biology and drug discovery? (Journal article)
Foulkes, D. M., Byrne, D. P., Bailey, F. P., & Eyers, P. A. (2015). Tribbles pseudokinases: novel targets for chemical biology and drug discovery?. BIOCHEMICAL SOCIETY TRANSACTIONS, 43, 1095-1103. doi:10.1042/BST20150109DOI: 10.1042/BST20150109
Breakdown of albumin and haemalbumin by the cysteine protease interpain A, an albuminase of <i>Prevotella intermedia</i> (Journal article)
Byrne, D. P., Manandhar, S. P., Potempa, J., & Smalley, J. W. (2015). Breakdown of albumin and haemalbumin by the cysteine protease interpain A, an albuminase of <i>Prevotella intermedia</i>. BMC MICROBIOLOGY, 15. doi:10.1186/s12866-015-0516-3DOI: 10.1186/s12866-015-0516-3
The Tribbles 2 (TRB2) pseudokinase binds to ATP and autophosphorylates in a metal-independent manner (Journal article)
Bailey, F., Byrne, D., Oruganty, K., Eyers, C., Novotny, C. J., Shokat, K. M., . . . Eyers, P. (2015). The Tribbles 2 (TRB2) pseudokinase binds to ATP and autophosphorylates in a metal-independent manner. Biochemical Journal, 467(1), 47-62. doi:10.1042/BJ20141441DOI: 10.1042/BJ20141441
Pyocycanin, a Contributory Factor in Haem Acquisition and Virulence Enhancement of <i>Porphyromonas gingivalis</i> in the Lung (Journal article)
Benedyk, M., Byrne, D. P., Glowczyk, I., Potempa, J., Olczak, M., Olczak, T., & Smalley, J. W. (2015). Pyocycanin, a Contributory Factor in Haem Acquisition and Virulence Enhancement of <i>Porphyromonas gingivalis</i> in the Lung. PLOS ONE, 10(2). doi:10.1371/journal.pone.0118319DOI: 10.1371/journal.pone.0118319
Going for broke: targeting the human cancer pseudokinome (Journal article)
Bailey, F. P., Byrne, D. P., McSkimming, D., Kannan, N., & Eyers, P. A. (2015). Going for broke: targeting the human cancer pseudokinome. BIOCHEMICAL JOURNAL, 465, 195-211. doi:10.1042/BJ20141060DOI: 10.1042/BJ20141060
2014
Caveolae as specialized lipid rafts: Diverse roles in the regulation of potassium channel signalling (Chapter)
Byrne, D., & Dart, C. (2014). Caveolae as specialized lipid rafts: Diverse roles in the regulation of potassium channel signalling. In Lipid Rafts: Properties, Controversies and Roles in Signal Transduction (pp. 53-68).
2013
Evidence of mutualism between two periodontal pathogens: co-operative haem acquisition by the HmuY haemophore of Porphyromonas gingivalis and the cysteine protease interpain A (InpA) of Prevotella intermedia. (Journal article)
Byrne, D. P., Potempa, J., Olczak, T., & Smalley, J. W. (2013). Evidence of mutualism between two periodontal pathogens: co-operative haem acquisition by the HmuY haemophore of Porphyromonas gingivalis and the cysteine protease interpain A (InpA) of Prevotella intermedia.. Molecular oral microbiology, 28(3), 219-229. doi:10.1111/omi.12018DOI: 10.1111/omi.12018
2012
Evaluating Caveolin Interactions: Do Proteins Interact with the Caveolin Scaffolding Domain through a Widespread Aromatic Residue-Rich Motif? (Journal article)
Byrne, D. P., Dart, C., & Rigden, D. J. (2012). Evaluating Caveolin Interactions: Do Proteins Interact with the Caveolin Scaffolding Domain through a Widespread Aromatic Residue-Rich Motif?. PLOS ONE, 7(9). doi:10.1371/journal.pone.0044879DOI: 10.1371/journal.pone.0044879
2011
Smalley, J. W., Byrne, D. P., Birss, A. J., Wojtowicz, H., Sroka, A., Potempa, J., & Olczak, T. (2011). HmuY haemophore and gingipain proteases constitute a unique syntrophic system of haem acquisition by Porphyromonas gingivalis.. PloS one, 6(2), e17182. doi:10.1371/journal.pone.0017182DOI: 10.1371/journal.pone.0017182
2010
Haemin affects the growth and physiology of Prevotella intermedia (Journal article)
Marsh, P. D., Percival, R. S., Abourserwel, A., Birss, A. J., Byrne, D., Smalley, J. W., & Devine, D. A. (2010). Haemin affects the growth and physiology of Prevotella intermedia. J Dent Res, Spec I, Abstract ID#138208.
2009
Role of the cysteine protease interpain A of Prevotella intermedia in breakdown and release of haem from haemoglobin. (Journal article)
Byrne, D. P., Wawrzonek, K., Jaworska, A., Birss, A. J., Potempa, J., & Smalley, J. W. (2009). Role of the cysteine protease interpain A of Prevotella intermedia in breakdown and release of haem from haemoglobin.. The Biochemical journal, 425(1), 257-264. doi:10.1042/bj20090343DOI: 10.1042/bj20090343
