Prof Claire Eyers BSc, PhD, FRSC
Professor of Biological Mass Spectrometry Biochemistry
- +44 (0)151 795 4424
- Work email Claire.Eyers@liverpool.ac.uk
- Personal Websitehttp://www.liv.ac.uk/pfg/
Publications
Selected Publications
- DNA Binding and Phosphorylation Regulate the Core Structure of the NF-B p50 Transcription Factor (Journal article - 2019)
- Covalent inhibitors of EGFR family protein kinases induce degradation of human Tribbles 2 (TRIB2) pseudokinase in cancer cells (Journal article - 2018)
- New tools for evaluating protein tyrosine sulfation: tyrosylprotein sulfotransferases (TPSTs) are novel targets for RAF protein kinase inhibitors (Journal article - 2018)
- Understanding protein-drug interactions using ion mobility-mass spectrometry (Journal article - 2018)
- Strong anion exchange-mediated phosphoproteomics reveals extensive human non-canonical phosphorylation (Journal article - 2019)
2020
Oxygen-dependent changes in HIF binding partners and post-translational modifications regulate stability and transcriptional activity (Journal article)
Daly, L., Brownridge, P., Sée, V., & Eyers, C. (2020). Oxygen-dependent changes in HIF binding partners and post-translational modifications regulate stability and transcriptional activity. doi:10.1101/2020.11.12.379768
Characterisation of protease activity during SARS-CoV-2 infection identifies novel viral cleavage sites and cellular targets for drug repurposing (Journal article)
Meyer, B., Chiaravalli, J., Brownridge, P., Bryne, D., Daly, L., Agou, F., . . . Emmott, E. (2020). Characterisation of protease activity during SARS-CoV-2 infection identifies novel viral cleavage sites and cellular targets for drug repurposing. doi:10.1101/2020.09.16.297945
Temporal modulation of the NF-?B RelA network in response to different types of DNA damage (Journal article)
Campbell, A., Franco, C. F., Su, L. -I., Perkins, S., Jones, A., Brownridge, P., . . . Eyers, C. (2020). Temporal modulation of the NF-?B RelA network in response to different types of DNA damage. doi:10.1101/2020.08.11.246504
Aurora A regulation by reversible cysteine oxidation reveals evolutionarily conserved redox control of Ser/Thr protein kinase activity (Journal article)
Byrne, D. P., Shrestha, S., Galler, M., Cao, M., Daly, L. A., Campbell, A. E., . . . Eyers, P. A. (2020). Aurora A regulation by reversible cysteine oxidation reveals evolutionarily conserved redox control of Ser/Thr protein kinase activity. Science Signaling, 13(639). doi:10.1126/scisignal.aax2713
Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signalling networks using SILAC-based phosphoproteomics (Journal article)
Byrne, D. P., Clarke, C. J., Brownridge, P. J., Kalyuzhnyy, A., Perkins, S., Campbell, A., . . . Eyers, C. E. (2020). Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signalling networks using SILAC-based phosphoproteomics. The Biochemical journal, 477(13), 2451-2475. doi:10.1042/bcj20200309
Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signaling networks using SILAC-based phosphoproteomics (Journal article)
Byrne, D., Clarke, C., Brownridge, P., Kalyuzhnyy, A., Perkins, S., Campbell, A., . . . Eyers, C. (2020). Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signaling networks using SILAC-based phosphoproteomics. doi:10.1101/2020.05.22.110767
Quantitative Proteomics of Enriched Esophageal and Gut Tissues from the Human Blood Fluke Schistosoma mansoni Pinpoints Secreted Proteins for Vaccine Development. (Journal article)
Neves, L. X., Wilson, R. A., Brownridge, P., Harman, V. M., Holman, S. W., Beynon, R. J., . . . Castro-Borges, W. (2020). Quantitative Proteomics of Enriched Esophageal and Gut Tissues from the Human Blood Fluke Schistosoma mansoni Pinpoints Secreted Proteins for Vaccine Development. JOURNAL OF PROTEOME RESEARCH, 19(1), 314-326. doi:10.1021/acs.jproteome.9b00531
Covalent Aurora A regulation by the metabolic integrator coenzyme A (Journal article)
Tsuchiya, Y., Byrne, D. P., Burgess, S. G., Bormann, J., Bakovic, J., Huang, Y., . . . Gout, I. (2020). Covalent Aurora A regulation by the metabolic integrator coenzyme A. REDOX BIOLOGY, 28. doi:10.1016/j.redox.2019.101318
Determination of Phosphohistidine Stoichiometry in Histidine Kinases by Intact Mass Spectrometry. (Journal article)
Tomlinson, L. J., Clubbs Coldron, A. K. M., Eyers, P. A., & Eyers, C. E. (2020). Determination of Phosphohistidine Stoichiometry in Histidine Kinases by Intact Mass Spectrometry.. Methods in molecular biology (Clifton, N.J.), 2077, 83-91. doi:10.1007/978-1-4939-9884-5_6
High-Throughput Characterization of Histidine Phosphorylation Sites Using UPAX and Tandem Mass Spectrometry. (Chapter)
Hardman, G., & Eyers, C. E. (2020). High-Throughput Characterization of Histidine Phosphorylation Sites Using UPAX and Tandem Mass Spectrometry.. In Unknown Book (Vol. 2077, pp. 225-235). doi:10.1007/978-1-4939-9884-5_15
Ion Mobility-Mass Spectrometry to Evaluate the Effects of Protein Modification or Small Molecule Binding on Protein Dynamics. (Journal article)
Tomlinson, L. J., & Eyers, C. E. (2020). Ion Mobility-Mass Spectrometry to Evaluate the Effects of Protein Modification or Small Molecule Binding on Protein Dynamics.. Methods in molecular biology (Clifton, N.J.), 2084, 179-190. doi:10.1007/978-1-0716-0030-6_11
Preface (Book)
Preface (2014). . Royal Society of Chemistry. doi:10.1039/9781782626985-fp005
2019
Advancing Solutions to the Carbohydrate Sequencing Challenge. (Journal article)
Gray, C. J., Migas, L. G., Barran, P. E., Pagel, K., Seeberger, P. H., Eyers, C. E., . . . Flitsch, S. L. (2019). Advancing Solutions to the Carbohydrate Sequencing Challenge. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 141(37), 14463-14479. doi:10.1021/jacs.9b06406
Strong anion exchange-mediated phosphoproteomics reveals extensive human non-canonical phosphorylation (Journal article)
Hardman, G., Perkins, S., Brownridge, P., Clarke, C., Byrne, D., Campbell, A., . . . Eyers, C. (2019). Strong anion exchange-mediated phosphoproteomics reveals extensive human non-canonical phosphorylation. The EMBO Journal, 38. doi:10.15252/embj.2018100847
DNA Binding and Phosphorylation Regulate the Core Structure of the NF-B p50 Transcription Factor (Journal article)
Vonderach, M., Byrne, D. P., Barran, P. E., Eyers, P. A., & Eyers, C. E. (2019). DNA Binding and Phosphorylation Regulate the Core Structure of the NF-B p50 Transcription Factor. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 30(1), 128-138. doi:10.1007/s13361-018-1984-0
Myoblast Phosphoproteomics as a Tool to Investigate Global Signaling Events During Myogenesis. (Journal article)
Jones, F. K., Hardman, G. E., Ferries, S., Eyers, C. E., & Pisconti, A. (2019). Myoblast Phosphoproteomics as a Tool to Investigate Global Signaling Events During Myogenesis.. Methods in molecular biology (Clifton, N.J.), 1889, 301-317. doi:10.1007/978-1-4939-8897-6_18
2018
Covalent inhibitors of EGFR family protein kinases induce degradation of human Tribbles 2 (TRIB2) pseudokinase in cancer cells (Journal article)
Foulkes, D. M., Byrne, D. P., Yeung, W., Shrestha, S., Bailey, F. P., Ferries, S., . . . Eyers, P. A. (2018). Covalent inhibitors of EGFR family protein kinases induce degradation of human Tribbles 2 (TRIB2) pseudokinase in cancer cells. SCIENCE SIGNALING, 11(549), 14 pages. doi:10.1126/scisignal.aat7951
New tools for carbohydrate sulfation analysis: heparan sulfate 2-O-sulfotransferase (HS2ST) is a target for small-molecule protein kinase inhibitors (Journal article)
Byrne, D. P., Li, Y., Ramakrishnan, K., Barsukov, I. L., Yates, E. A., Eyers, C. E., . . . Eyers, P. A. (2018). New tools for carbohydrate sulfation analysis: heparan sulfate 2-O-sulfotransferase (HS2ST) is a target for small-molecule protein kinase inhibitors. BIOCHEMICAL JOURNAL, 475(15), 2417-2433. doi:10.1042/BCJ20180265
New tools for carbohydrate sulphation analysis: Heparan Sulphate 2-O-sulphotranserase (HS2ST) is a target for small molecule protein kinase inhibitors (Journal article)
Byrne, D. P., Li, Y., Ramakrishnan, K., Barsukov, I. L., Yates, E. A., Eyers, C. E., . . . Eyers, P. A. (n.d.). New tools for carbohydrate sulphation analysis: Heparan Sulphate 2-O-sulphotranserase (HS2ST) is a target for small molecule protein kinase inhibitors. doi:10.1101/296533
New tools for evaluating protein tyrosine sulfation: tyrosylprotein sulfotransferases (TPSTs) are novel targets for RAF protein kinase inhibitors (Journal article)
Byrne, D. P., Li, Y., Ngamlert, P., Ramakrishnan, K., Eyers, C. E., Wells, C., . . . Eyers, P. A. (2018). New tools for evaluating protein tyrosine sulfation: tyrosylprotein sulfotransferases (TPSTs) are novel targets for RAF protein kinase inhibitors. BIOCHEMICAL JOURNAL, 475(15), 2435-2455. doi:10.1042/BCJ20180266
Repurposing covalent EGFR/HER2 inhibitors for on-target degradation of human Tribbles 2 (TRIB2) pseudokinase (Journal article)
Foulkes, D., Byrne, D., Bailey, F., Ferries, S., Eyers, C., Keeshan, K., . . . Eyers, P. (2018). Repurposing covalent EGFR/HER2 inhibitors for on-target degradation of human Tribbles 2 (TRIB2) pseudokinase. doi:10.1101/305243
A quantitative and temporal map of proteostasis during heat shock in Saccharomyces cerevisiae (Journal article)
Jarnuczak, A. F., Albornoz, M. G., Eyers, C. E., Grant, C. M., & Hubbard, S. J. (2018). A quantitative and temporal map of proteostasis during heat shock in Saccharomyces cerevisiae. MOLECULAR OMICS, 14(1), 37-52. doi:10.1039/c7mo00050b
Understanding protein-drug interactions using ion mobility-mass spectrometry (Journal article)
Eyers, C. E., Vonderach, M., Ferries, S., Jeacock, K., & Eyers, P. A. (2018). Understanding protein-drug interactions using ion mobility-mass spectrometry. CURRENT OPINION IN CHEMICAL BIOLOGY, 42, 167-176. doi:10.1016/j.cbpa.2017.12.013
2017
Plk4 and Aurora A cooperate in the initiation of acentriolar spindle assembly in mammalian oocytes (Journal article)
Bury, L., Coelho, P. A., Simeone, A., Ferries, S., Eyers, C. E., Eyers, P. A., . . . Glover, D. M. (2017). Plk4 and Aurora A cooperate in the initiation of acentriolar spindle assembly in mammalian oocytes. JOURNAL OF CELL BIOLOGY, 216(11), 3571-3590. doi:10.1083/jcb.201606077
Extensive non-canonical phosphorylation in human cells revealed using strong-anion exchange-mediated phosphoproteomics (Journal article)
Hardman, G., Perkins, S., Ruan, Z., Kannan, N., Brownridge, P., Byrne, D., . . . Eyers, C. E. (n.d.). Extensive non-canonical phosphorylation in human cells revealed using strong-anion exchange-mediated phosphoproteomics. BioRxiv. doi:10.1101/202820
Evaluation of Parameters for Confident Phosphorylation Site Localization using an Orbitrap Fusion Tribrid Mass Spectrometer (Journal article)
Ferries., Perkins, S., Brownridge., Campbell, A., Eyers, P., Jones, A., & Eyers, C. E. (2017). Evaluation of Parameters for Confident Phosphorylation Site Localization using an Orbitrap Fusion Tribrid Mass Spectrometer. Journal of Proteome Research, 16(9), 3448-3459. doi:10.1021/acs.jproteome.7b00337
Local protein kinase A action proceeds through intact holoenzymes (Journal article)
Smith, F. D., Esseltine, J. L., Nygren, P. J., Veesler, D., Byrne, D. P., Vonderach, M., . . . Scott, J. D. (2017). Local protein kinase A action proceeds through intact holoenzymes. Science, 356(6344), 1288-1293. doi:10.1126/science.aaj1669
Bottom-up elucidation of glycosidic bond stereochemistry (Journal article)
Gray, C., Schindler, B., Migas, L., Picmanova, M., Allouche, A., Green, A., . . . Flitsch, S. (2017). Bottom-up elucidation of glycosidic bond stereochemistry. Analytical Chemistry, 89(08), 4540-4549. doi:10.1021/acs.analchem.6b04998
Label-Free Discovery Array Platform for the Characterization of Glycan Binding Proteins and Glycoproteins (Journal article)
Gray, C. J., Sanchez-Ruiz, A., Sardzikova, I., Ahmed, Y. A., Miller, R. L., Reyes Martinez, J. E., . . . Flitsch, S. L. (2017). Label-Free Discovery Array Platform for the Characterization of Glycan Binding Proteins and Glycoproteins. ANALYTICAL CHEMISTRY, 89(8), 4444-4451. doi:10.1021/acs.analchem.6b04122
DOSCATs: Double standards for protein quantification (Journal article)
Bennett, R. J., Simpson, D. M., Holman, S. W., Ryan, S., Brownridge, P., Eyers, C. E., . . . Beynon, R. J. (2017). DOSCATs: Double standards for protein quantification. Scientific Reports, 7. doi:10.1038/srep45570
2016
KinView: A visual comparative sequence analysis tool for integrated kinome research (Journal article)
Skimming, D., Dastgheib, S., Baffi, T., Byrne, D., Ferries, S., Scott, S., . . . Kannan, N. (2016). KinView: A visual comparative sequence analysis tool for integrated kinome research. Molecular BioSystems, (12), 3651-3665. doi:10.1039/C6MB00466K
Human CDK18 promotes replication stress signaling and genome stability (Journal article)
Barone, G., Staples, C., Ganesh, A., Patterson, K., Byrne, D., Myers, K., . . . Collis, S. (2016). Human CDK18 promotes replication stress signaling and genome stability. Nucleic Acids Research, 44(18), 8772-8785. doi:10.1093/nar/gkw615
cAMP-dependent protein kinase (PKA) complexes probed by complementary differential scanning fluorimetry and ion mobility-mass spectrometry. (Journal article)
Byrne, D. P., Vonderach, M., Ferries, S., Brownridge, P. J., Eyers, C. E., & Eyers, P. A. (2016). cAMP-dependent protein kinase (PKA) complexes probed by complementary differential scanning fluorimetry and ion mobility-mass spectrometry. BIOCHEMICAL JOURNAL, 473, 3159-3175. doi:10.1042/BCJ20160648
Analysis of Intrinsic Peptide Detectability via Integrated Label-Free and SRM-Based Absolute Quantitative Proteomics (Journal article)
Jarnuczak, A. F., Lee, D. C. H., Lawless, C., Holman, S. W., Eyers, C. E., & Hubbard, S. J. (2016). Analysis of Intrinsic Peptide Detectability via Integrated Label-Free and SRM-Based Absolute Quantitative Proteomics. JOURNAL OF PROTEOME RESEARCH, 15(9), 2945-2959. doi:10.1021/acs.jproteome.6b00048
Absolute protein quantification of the yeast chaperome under conditions of heat shock. (Journal article)
Mackenzie, R. J., Lawless, C., Holman, S., Lanthaler, K., Beynon, R. J., Grant, C. M., . . . Eyers, C. E. (2016). Absolute protein quantification of the yeast chaperome under conditions of heat shock. Proteomics, 16(15-16), 2128-2140. doi:10.1002/pmic.201500503
Applications of ion mobility mass spectrometry for high throughput, high resolution glycan analysis (Journal article)
Gray, C. J., Thomas, B., Upton, R., Migas, L. G., Eyers, C. E., Barran, P. E., & Flitsch, S. L. (2016). Applications of ion mobility mass spectrometry for high throughput, high resolution glycan analysis. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1860(8), 1688-1709. doi:10.1016/j.bbagen.2016.02.003
Dynamic phosphorylation of RelA on Ser42 and Ser45 in response to TNFa stimulation regulates DNA binding and transcription (Journal article)
lanucara, F., lam, C., Mann, J., Monie, T., Colombo, S., Holman, S., . . . Eyers, C. E. (2016). Dynamic phosphorylation of RelA on Ser42 and Ser45 in response to TNFa stimulation regulates DNA binding and transcription. Open Biology, 6(7). doi:10.1098/rsob.160055
RePLiCal: A QconCAT Protein for Retention Time Standardization in Proteomics Studies (Journal article)
Holman, S. W., McLean, L., & Eyers, C. E. (2016). RePLiCal: A QconCAT Protein for Retention Time Standardization in Proteomics Studies. JOURNAL OF PROTEOME RESEARCH, 15(3), 1090-1102. doi:10.1021/acs.jproteome.5b00988
Direct and Absolute Quantification of over 1800 Yeast Proteins via Selected Reaction Monitoring (Journal article)
Lawless, C., Holman, S. W., Brownridge, P., Lanthaler, K., Harman, V. M., Watkins, R., . . . Hubbard, S. J. (2016). Direct and Absolute Quantification of over 1800 Yeast Proteins via Selected Reaction Monitoring. MOLECULAR & CELLULAR PROTEOMICS, 15(4), 1309-1322. doi:10.1074/mcp.M115.054288
2015
Evaluation of dimethyl sulfoxide (DMSO) as a mobile phase additive during top 3 label-free quantitative proteomics (Journal article)
Strzelecka, D., Holman, S. W., & Eyers, C. E. (2015). Evaluation of dimethyl sulfoxide (DMSO) as a mobile phase additive during top 3 label-free quantitative proteomics. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 391, 157-160. doi:10.1016/j.ijms.2015.07.004
In honour of Professor Simon Gaskell's 65th birthday (Journal article)
Eyers, C. E., & Wysocki, V. H. (2015). In honour of Professor Simon Gaskell's 65th birthday. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 391, 1. doi:10.1016/j.ijms.2015.10.013
Mass spectrometry for structural analysis and quantification of the Major Urinary Proteins of the house mouse (Journal article)
Beynon, R. J., Armstrong, S. D., Claydon, A. J., Davidson, A. J., Eyers, C. E., Langridge, J. I., . . . Woolerton, Y. E. (2015). Mass spectrometry for structural analysis and quantification of the Major Urinary Proteins of the house mouse. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 391, 146-156. doi:10.1016/j.ijms.2015.07.026
Focus on Quantitative Proteomics (Journal article)
Lilley, K. S., Beynon, R. J., Eyers, C. E., & Hubbard, S. J. (2015). Focus on Quantitative Proteomics. PROTEOMICS, 15(18), 3101-3103. doi:10.1002/pmic.201570163
Quantitative proteomics and network analysis of SSA1 and SSB1 deletion mutants reveals robustness of chaperone HSP70 network in Saccharomyces cerevisiae (Journal article)
Jamuczak, A. E., Eyers, C. E., Schwartz, J. -M., Grant, C. M., & Hubbard, S. J. (2015). Quantitative proteomics and network analysis of SSA1 and SSB1 deletion mutants reveals robustness of chaperone HSP70 network in Saccharomyces cerevisiae. PROTEOMICS, 15(18), 3126-3139. doi:10.1002/pmic.201400527
The Tribbles 2 (TRB2) pseudokinase binds to ATP and autophosphorylates in a metal-independent manner (Journal article)
Bailey, F., Byrne, D., Oruganty, K., Eyers, C., Novotny, C. J., Shokat, K. M., . . . Eyers, P. (2015). The Tribbles 2 (TRB2) pseudokinase binds to ATP and autophosphorylates in a metal-independent manner. Biochemical Journal, 467(1), 47-62. doi:10.1042/BJ20141441
Protein Structure (Journal article)
Barran, P., Cooper, H., & Eyers, C. (2015). Protein Structure. PROTEOMICS, 15(16), 2731-2732. doi:10.1002/pmic.201570143
2014
Eukaryotic Elongation Factor 2 Kinase Activity Is Controlled by Multiple Inputs from Oncogenic Signaling (Journal article)
Wang, X., Regufe da Mota, S., Rui, L., Moore, C. E., XIe, J., Lanucara, F., . . . Proud, C. G. (2014). Eukaryotic Elongation Factor 2 Kinase Activity Is Controlled by Multiple Inputs from Oncogenic Signaling. Molecular and Cellular Biology, 34(22), 4088-4103. doi:10.1128/MCB.01035-14
Peptide scrambling during collision-induced dissociation is influenced by N-terminal residue basicity (Journal article)
Chawner, R., Holman, S. W., Gaskell, S. J., & Eyers, C. E. (2014). Peptide scrambling during collision-induced dissociation is influenced by N-terminal residue basicity. Journal of the American Society of Mass Spectrometry, 25(11), 1927-1938. doi:10.1021/jasms.8b04652
Gas-phase intermolecular phosphate transfer within a phosphohistidine phosphopeptide dimer (Journal article)
Gonzalez-Sanchez, M. -B., Lanucara, F., Hardman, G. E., & Eyers, C. E. (2014). Gas-phase intermolecular phosphate transfer within a phosphohistidine phosphopeptide dimer. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 367, 28-34. doi:10.1016/j.ijms.2014.04.015
Probing the exposure of the phosphate group in modified amino acids and peptides by ion-molecule reactions with triethoxyborane in Fourier transform ion cyclotron resonance mass spectrometry (Journal article)
Lanucara, F., Fornarini, S., Eyers, C. E., & Crestoni, M. E. (2014). Probing the exposure of the phosphate group in modified amino acids and peptides by ion-molecule reactions with triethoxyborane in Fourier transform ion cyclotron resonance mass spectrometry. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 28(10), 1107-1116. doi:10.1002/rcm.6884
Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing (vol 6, pg 65, 2014) (Journal article)
Both, P., Green, A. P., Gray, C. J., Sardzik, R., Voglmeir, J., Fontana, C., . . . Eyers, C. E. (2014). Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing (vol 6, pg 65, 2014). NATURE CHEMISTRY, 6(4), 368. doi:10.1038/NCHEM.1901
Unblocking the Sink: Improved CID-Based Analysis of Phosphorylated Peptides by Enzymatic Removal of the Basic C-Terminal Residue (Journal article)
Lanucara, F., Lee, D. C. H., & Eyers, C. E. (2014). Unblocking the Sink: Improved CID-Based Analysis of Phosphorylated Peptides by Enzymatic Removal of the Basic C-Terminal Residue. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 25(2), 214-225. doi:10.1007/s13361-013-0770-2
Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing (Journal article)
Both, P., Green, A. P., Gray, C. J., Sardzik, R., Voglmeir, J., Fontana, C., . . . Eyers, C. E. (2014). Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing. NATURE CHEMISTRY, 6(1), 65-74. doi:10.1038/NCHEM.1817
Probing the exposure of the phosphate group in modified amino acids and peptides by ion-molecule reactions with triethoxyborane in FT-ICR mass spectrometry (Journal article)
Lanucara, F., Fornarini, S., Eyers, C. E., & Crestoni, M. (2014). Probing the exposure of the phosphate group in modified amino acids and peptides by ion-molecule reactions with triethoxyborane in FT-ICR mass spectrometry. Rapid Communications in Mass Spectrometry.
Quantitative Proteomics (Book)
Quantitative Proteomics (2014). . Royal Society of Chemistry. doi:10.1039/9781782626985
The power of ion mobility-mass spectrometry for structural characterization and the study of conformational dynamics (Journal article)
Lanucara, F., Holman, S. W., Gray, C. J., & Eyers, C. E. (2014). The power of ion mobility-mass spectrometry for structural characterization and the study of conformational dynamics. NATURE CHEMISTRY, 6(4), 281-294. doi:10.1038/NCHEM.1889
2013
ChemInform Abstract: Enzymatic Reactions on Immobilized Substrates (Journal article)
Gray, C. J., Weissenborn, M. J., Eyers, C. E., & Flitsch, S. L. (2013). ChemInform Abstract: Enzymatic Reactions on Immobilized Substrates. ChemInform, 44(40), no. doi:10.1002/chin.201340262
Enzymatic reactions on immobilised substrates (Journal article)
Gray, C. J., Weissenborn, M. J., Eyers, C. E., & Flitsch, S. L. (2013). Enzymatic reactions on immobilised substrates. CHEMICAL SOCIETY REVIEWS, 42(15), 6378-6405. doi:10.1039/c3cs60018a
Attempting to rewrite History: challenges with the analysis of histidine-phosphorylated peptides (Journal article)
Gonzalez-Sanchez, M. -B., Lanucara, F., Helm, M., & Eyers, C. E. (2013). Attempting to rewrite History: challenges with the analysis of histidine-phosphorylated peptides. BIOCHEMICAL SOCIETY TRANSACTIONS, 41, 1089-1095. doi:10.1042/BST20130072
The nitrosated bile acid DNA lesion O-6-carboxymethylguanine is a substrate for the human DNA repair protein O-6-methylguanine-DNA methyltransferase (Journal article)
Senthong, P., Millington, C. L., Wilkinson, O. J., Marriott, A. S., Watson, A. J., Reamtong, O., . . . Povey, A. C. (2013). The nitrosated bile acid DNA lesion O-6-carboxymethylguanine is a substrate for the human DNA repair protein O-6-methylguanine-DNA methyltransferase. NUCLEIC ACIDS RESEARCH, 41(5), 3047-3055. doi:10.1093/nar/gks1476
A novel approach to the analysis of SUMOylation with the independent use of trypsin and elastase digestion followed by database searching utilising consecutive residue addition to lysine (Journal article)
Chicooree, N., Griffiths, J. R., Connolly, Y., Tan, C. -T., Malliri, A., Eyers, C. E., & Smith, D. L. (2013). A novel approach to the analysis of SUMOylation with the independent use of trypsin and elastase digestion followed by database searching utilising consecutive residue addition to lysine. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 27(1), 127-134. doi:10.1002/rcm.6425
Top-down mass spectrometry for the analysis of combinatorial post-translational modifications (Journal article)
Lanucara, F., & Eyers, C. E. (2013). Top-down mass spectrometry for the analysis of combinatorial post-translational modifications. MASS SPECTROMETRY REVIEWS, 32(1), 27-42. doi:10.1002/mas.21348
2012
Dual purpose S-trityl-linkers for glycoarray fabrication on both polystyrene and gold (Journal article)
Wehner, J. W., Weissenborn, M. J., Hartmann, M., Gray, C. J., Sardzik, R., Eyers, C. E., . . . Lindhorst, T. K. (2012). Dual purpose S-trityl-linkers for glycoarray fabrication on both polystyrene and gold. ORGANIC & BIOMOLECULAR CHEMISTRY, 10(44), 8919-8926. doi:10.1039/c2ob26118a
QconCAT Standard for Calibration of Ion Mobility-Mass Spectrometry Systems (Journal article)
Chawner, R., McCullough, B., Giles, K., Barran, P. E., Gaskell, S. J., & Eyers, C. E. (2012). QconCAT Standard for Calibration of Ion Mobility-Mass Spectrometry Systems. JOURNAL OF PROTEOME RESEARCH, 11(11), 5564-5572. doi:10.1021/pr3005327
The N-Methylated Peptide SEN304 Powerfully Inhibits A beta(1-42) Toxicity by Perturbing Oligomer Formation (Journal article)
Amijee, H., Bate, C., Williams, A., Virdee, J., Jeggo, R., Spanswick, D., . . . Doig, A. J. (2012). The N-Methylated Peptide SEN304 Powerfully Inhibits A beta(1-42) Toxicity by Perturbing Oligomer Formation. BIOCHEMISTRY, 51(42), 8338-8352. doi:10.1021/bi300415v
The Nitrosated Bile Acid DNA Lesion, O-6-carboxymethylguanine, Is a Substrate for Human O-6-alkylguanine DNA Alkyltransferase (Conference Paper)
Senthong, P., Williams, D. M., Wilkinson, O., Millington, C., Marriott, A., Watson, A. J., . . . Povey, A. C. (2012). The Nitrosated Bile Acid DNA Lesion, O-6-carboxymethylguanine, Is a Substrate for Human O-6-alkylguanine DNA Alkyltransferase. In ENVIRONMENTAL AND MOLECULAR MUTAGENESIS Vol. 53 (pp. S36). Retrieved from http://gateway.webofknowledge.com/
The use of selected reaction monitoring in quantitative proteomics (Journal article)
Holman, S. W., Sims, P. F. G., & Eyers, C. E. (2012). The use of selected reaction monitoring in quantitative proteomics. BIOANALYSIS, 4(14), 1763-1786. doi:10.4155/BIO.12.126
Formation of carbohydrate-functionalised polystyrene and glass slides and their analysis by MALDI-TOF MS (Journal article)
Weissenborn, M. J., Wehner, J. W., Gray, C. J., Sardzik, R., Eyers, C. E., Lindhorst, T. K., & Flitsch, S. L. (2012). Formation of carbohydrate-functionalised polystyrene and glass slides and their analysis by MALDI-TOF MS. BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY, 8, 753-762. doi:10.3762/bjoc.8.86
The influence of a C-terminal basic residue on peptide fragmentation pathways (Journal article)
Chawner, R., Eyers, C. E., & Gaskell, S. J. (2012). The influence of a C-terminal basic residue on peptide fragmentation pathways. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 316, 284-291. doi:10.1016/j.ijms.2012.02.017
Proposal for a common nomenclature for peptide fragment ions generated following sequence scrambling during collision-induced dissociation (Journal article)
Chawner, R., Gaskell, S. J., & Eyers, C. E. (2012). Proposal for a common nomenclature for peptide fragment ions generated following sequence scrambling during collision-induced dissociation. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 26(2), 205-206. doi:10.1002/rcm.5294
2011
Absolute Quantification of the Glycolytic Pathway in Yeast: DEPLOYMENT OF A COMPLETE QconCAT APPROACH (Journal article)
Carroll, K. M., Simpson, D. M., Eyers, C. E., Knight, C. G., Brownridge, P., Dunn, W. B., . . . Beynon, R. J. (2011). Absolute Quantification of the Glycolytic Pathway in Yeast: DEPLOYMENT OF A COMPLETE QconCAT APPROACH. MOLECULAR & CELLULAR PROTEOMICS, 10(12). doi:10.1074/mcp.M111.007633
CONSeQuence: Prediction of Reference Peptides for Absolute Quantitative Proteomics Using Consensus Machine Learning Approaches (Journal article)
Eyers, C. E., Lawless, C., Wedge, D. C., Lau, K. W., Gaskell, S. J., & Hubbard, S. J. (2011). CONSeQuence: Prediction of Reference Peptides for Absolute Quantitative Proteomics Using Consensus Machine Learning Approaches. MOLECULAR & CELLULAR PROTEOMICS, 10(11). doi:10.1074/mcp.M110.003384
Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes (Journal article)
Drummond, S. P., Hildyard, J., Firczuk, H., Reamtong, O., Li, N., Kannambath, S., . . . McCarthy, J. E. G. (2011). Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes. NUCLEIC ACIDS RESEARCH, 39(17), 7764-7774. doi:10.1093/nar/gkr474
Identification of a Lacosamide Binding Protein Using an Affinity Bait and Chemical Reporter Strategy: 14-3-3 zeta (Journal article)
Park, K. D., Kim, D., Reamtong, O., Eyers, C., Gaskell, S. J., Liu, R., & Kohn, H. (2011). Identification of a Lacosamide Binding Protein Using an Affinity Bait and Chemical Reporter Strategy: 14-3-3 zeta. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 133(29), 11320-11330. doi:10.1021/ja2034156
Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes (Journal article)
Drummond, S. P., Hildyard, J., Firczuk, H., Reamtong, O., Li, N., Kannambath, S., . . . McCarthy, J. E. G. (2011). Diauxic shift-dependent relocalization of decapping activators Dhh1 and Pat1 to polysomal complexes. Nucleic acids research, 39(17), 7764-7774.
MASS SPECTROMETRIC-BASED QUANTITATIVE PROTEOMICS USING SILAC (Journal article)
Lanucara, F., & Eyers, C. E. (2011). MASS SPECTROMETRIC-BASED QUANTITATIVE PROTEOMICS USING SILAC. METHODS IN ENZYMOLOGY, VOL 500, 500, 133-150. doi:10.1016/B978-0-12-385118-5.00008-6
QUANTIFICATION OF PROTEINS AND THEIR MODIFICATIONS USING QCONCAT TECHNOLOGY (Journal article)
Carroll, K. M., Lanucara, F., & Eyers, C. E. (2011). QUANTIFICATION OF PROTEINS AND THEIR MODIFICATIONS USING QCONCAT TECHNOLOGY. METHODS IN ENZYMOLOGY, VOL 500, 500, 113-131. doi:10.1016/B978-0-12-385118-5.00007-4
2010
Analysis of post-translational modifications by LC-MS/MS. (Journal article)
Johnson, H., & Eyers, C. E. (2010). Analysis of post-translational modifications by LC-MS/MS.. Methods in molecular biology (Clifton, N.J.), 658, 93-108. doi:10.1007/978-1-60761-780-8_5
2009
Rigorous Determination of the Stoichiometry of Protein Phosphorylation Using Mass Spectrometry (Journal article)
Johnson, H., Eyers, C. E., Eyers, P. A., Beynon, R. J., & Gaskell, S. J. (2009). Rigorous Determination of the Stoichiometry of Protein Phosphorylation Using Mass Spectrometry. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 20(12), 2211-2220. doi:10.1016/j.jasms.2009.08.009
Rigorous determination of the stoichiometry of protein phosphorylation using mass spectrometry (Journal article)
Johnson, H., Eyers, C. E., Eyers, P. A., Beynon, R. J., & Gaskell, S. J. (2009). Rigorous determination of the stoichiometry of protein phosphorylation using mass spectrometry. Journal of the American Society for Mass Spectrometry, 20(12), 2211-2220. doi:10.1016/j.jasms.2009.08.009
Lacosamide Isothiocyanate-Based Agents: Novel Agents To Target and Identify Lacosamide Receptors (Journal article)
Park, K. D., Morieux, P., Salome, C., Cotten, S. W., Reamtong, O., Eyers, C., . . . Kohn, H. (2009). Lacosamide Isothiocyanate-Based Agents: Novel Agents To Target and Identify Lacosamide Receptors. JOURNAL OF MEDICINAL CHEMISTRY, 52(21), 6897-6911. doi:10.1021/jm9012054
Universal sample preparation method for proteome analysis (Journal article)
Wisniewski, J. R., Zougman, A., Nagaraj, N., & Mann, M. (2009). Universal sample preparation method for proteome analysis. Nature Methods, 6(5), 359-362. doi:10.1038/nmeth.1322
2008
QCAL - a novel standard for assessing instrument conditions for proteome analysis (Journal article)
Eyers, C. E., Simpson, D. M., Wong, S. C. C., Beynon, R. J., & Gaskell, S. J. (2008). QCAL - a novel standard for assessing instrument conditions for proteome analysis. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 19(9), 1275-1280. doi:10.1016/j.jasms.2008.05.019
QCAL—a Novel Standard for Assessing Instrument Conditions for Proteome Analysis (Journal article)
EYERS, C., SIMPSON, D., WONG, S., BEYNON, R., & GASKELL, S. (2008). QCAL—a Novel Standard for Assessing Instrument Conditions for Proteome Analysis. Journal of the American Society for Mass Spectrometry, 19(9), 1275-1280. doi:10.1016/j.jasms.2008.05.019
All systems are go. (Journal article)
Eyers, C. E., & Reamtong, O. (2008). All systems are go.. Genome biology, 9(5), 307. doi:10.1186/gb-2008-9-5-307
Mass Spectrometry to Identify Posttranslational Modifications (Journal article)
Eyers, C. E., & Gaskell, S. J. (n.d.). Mass Spectrometry to Identify Posttranslational Modifications. doi:10.1002/9780470048672.wecb469
Proteomic analysis of the adaptation of the host NS0 myeloma cell line to a protein-free medium (Journal article)
de la Luz-Hernandez, K. R., Rojas-del, C. L., Victores-Sarasola, S., Lage-Castellanos, A. N., Eyers, C., Hart, S., . . . Gaskell, S. (2008). Proteomic analysis of the adaptation of the host NS0 myeloma cell line to a protein-free medium. Biotecnologia Aplicada, 24(03), y04.
2007
Peptide Detectability following ESI Mass Spectrometry: Prediction using Genetic Programming (Conference Paper)
Wedge, D. C., Kell, D. B., Gaskell, S. J., Lau, K. W., Hubbard, S. J., Eyers, C., & ACM. (2007). Peptide Detectability following ESI Mass Spectrometry: Prediction using Genetic Programming. In GECCO 2007: GENETIC AND EVOLUTIONARY COMPUTATION CONFERENCE, VOL 1 AND 2 (pp. 2219-+). Retrieved from http://gateway.webofknowledge.com/
Proteomic analysis of the adaptation of the host NSO myeloma cell line to a protein-free medium (Journal article)
De La Luz-Hernández, K. R., Rojas-Del Calvo, L., Victores-Sarasola, S., Lage-Castellanos, A., Eyers, C., Hart, S., . . . Gaskell, S. (2007). Proteomic analysis of the adaptation of the host NSO myeloma cell line to a protein-free medium. Biotecnologia Aplicada, 24(3-4), 215-223.
2005
The phosphorylation of CapZ-interacting protein (CapZIP) by stress-activated protein kinases triggers its dissociation from CapZ (Journal article)
Eyers, C. E., McNeill, H., Knebel, A., Morrice, N., Arthur, S. J. C., Cuenda, A., & Cohen, P. (2005). The phosphorylation of CapZ-interacting protein (CapZIP) by stress-activated protein kinases triggers its dissociation from CapZ. BIOCHEMICAL JOURNAL, 389, 127-135. doi:10.1042/BJ20050387
The phosphorylation of CapZ-interacting protein (CapZIP) by stress-activated protein kinases triggers its dissociation from CapZ. (Journal article)
Eyers, C. E., McNeill, H., Knebel, A., Morrice, N., Arthur, S. J. C., Cuenda, A., & Cohen, P. (2005). The phosphorylation of CapZ-interacting protein (CapZIP) by stress-activated protein kinases triggers its dissociation from CapZ.. The Biochemical journal, 389(Pt 1), 127-135. doi:10.1042/bj20050387
Loss of acetylation at Lys16 and trimethylation at Lys20 of histone H4 is a common hallmark of human cancer (Journal article)
Fraga, M. F., Ballestar, E., Villar-Garea, A., Boix-Chornet, M., Espada, J., Schotta, G., . . . Esteller, M. (2005). Loss of acetylation at Lys16 and trimethylation at Lys20 of histone H4 is a common hallmark of human cancer. NATURE GENETICS, 37(4), 391-400. doi:10.1038/ng1531
2003
Identification of novel phosphorylation sites on Xenopus laevis aurora A and analysis of phosphopeptide enrichment by immobilized metal-affinity chromatography (Journal article)
Haydon, C. E., Eyers, P. A., Aveline-Wolf, L. D., Resing, K. A., Maller, J. L., & Ahn, N. G. (2003). Identification of novel phosphorylation sites on Xenopus laevis aurora A and analysis of phosphopeptide enrichment by immobilized metal-affinity chromatography. MOLECULAR & CELLULAR PROTEOMICS, 2(10), 1055-1067. doi:10.1074/mcp.M300054-MCP200
The characterization of protein post-translational modifications by mass spectrometry (Journal article)
Schweppe, R. E., Haydon, C. E., Lewis, T. S., Resing, K. A., & Ahn, N. G. (2003). The characterization of protein post-translational modifications by mass spectrometry. ACCOUNTS OF CHEMICAL RESEARCH, 36(6), 453-461. doi:10.1021/ar020143l
The characterization of protein post-translational modifications by mass spectrometry. (Journal article)
Schweppe, R. E., Haydon, C. E., Lewis, T. S., Resing, K. A., & Ahn, N. G. (2003). The characterization of protein post-translational modifications by mass spectrometry.. Accounts of chemical research, 36(6), 453-461. doi:10.1021/ar020143l
2002
Stress-induced regulation of eukaryotic elongation factor 2 kinase by SB 203580-sensitive and -insensitive pathways (Journal article)
Knebel, A., Haydon, C. E., Morrice, N., & Cohen, P. (2002). Stress-induced regulation of eukaryotic elongation factor 2 kinase by SB 203580-sensitive and -insensitive pathways. BIOCHEMICAL JOURNAL, 367, 525-532. doi:10.1042/BJ20020916
Stress-induced regulation of eukaryotic elongation factor 2 kinase by SB 203580-sensitive and -insensitive pathways (Journal article)
KNEBEL, A., HAYDON, C. E., MORRICE, N., & COHEN, P. (2002). Stress-induced regulation of eukaryotic elongation factor 2 kinase by SB 203580-sensitive and -insensitive pathways. Biochemical Journal, 367(2), 525-532. doi:10.1042/bj20020916
Identification of a phosphorylation site on skeletal muscle myosin light chain kinase that becomes phosphorylated during muscle contraction (Journal article)
Haydon, C. E., Watt, P. W., Morrice, N., Knebel, A., Gaestel, M., & Cohen, P. (2002). Identification of a phosphorylation site on skeletal muscle myosin light chain kinase that becomes phosphorylated during muscle contraction. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 397(2), 224-231. doi:10.1006/abbi.2001.2625