Module Details

The information contained in this module specification was correct at the time of publication but may be subject to change, either during the session because of unforeseen circumstances, or following review of the module at the end of the session. Queries about the module should be directed to the member of staff with responsibility for the module.
Title Applied Analytical Chemistry
Code CHEM286
Coordinator Dr K Luzyanin
Year CATS Level Semester CATS Value
Session 2021-22 Level 5 FHEQ Second Semester 7.5

Pre-requisites before taking this module (or general academic requirements):

CHEM170 Introductory Spectroscopy 


To provide students with an understanding of the applications of various analytical techniques and their role in modern research. This module will demonstrate the fundamental theoretical principles of selected instrumental analytical techniques (NMR spectroscopy, mass-spectrometry, ICP-OE(MS) spectroscopy, separation and hyphenated techniques) in the context of their roles in industrial and academic research, to include chemical and pharmaceutical analysis.

Learning Outcomes

(LO1) To be able to recognise the current trends in the application of the instrumental analytical methods

(LO2) Demonstrate awareness of the theoretical concepts of NMR spectroscopy, Mass-Spectrometry, Chromatography, hyphenated techniques GC/HPLC-MS, and ICP-based methods

(LO3) To be able to choose an appropriate technique in order to evaluate the structure, properties and potential applications of materials, or a multi-technique approach to find the solution of a selected experimental problem

(S1) Students will develop their chemistry-related cognitive ability and skills, relating to intellectual tasks, including problem solving as required by the Chemistry subject benchmark statement.

(S2) Students will improve their confidence in scientific communication and develop presentation skills of analytical data

Teaching and Learning Strategies

Lectures (+ lecture-based tutorials, in-person): 17 hr

Coursework (workshops, 6 hr): (preparatory work in small groups remote and in person, followed by the delivery in-person during workshops). Three problem(case)-based learning workshops with summary presentation in small groups, 2 x 10% each (one workshop is training workshop and is assessed formatively). All workshops are peer-assessed by students and staff/demonstrators using feedback forms.
Problem- and case-based workshops are interlinked with the self-learning activities and include cooperative team work. Preparative work for the workshop is organized in a traditional problem-based learning style (groups of 4-6 students working cooperatively on solving of a case given). During workshops students refine their presentation upon formative feedback from staff and present and discuss the results to the audience composed of staff and students. This approach is therefore used as a teaching and learning method, import ant in developing LO1-LO3, S1, as well as presentation and communication skills (S2) and an assessment approach at the same time. Attendance is recorded.
*Lectures (+ lecture-based tutorials): 17 hr
*Workshops: 6 hr



Introduction (lectures 1 and 2)
1. Analytical chemistry: absolute and relative analytical methods, calibration (external calibration, standard additions, internal calibration), characteristics of an analytical procedure (accuracy, precision, specificity and selectivity, linearity range, detection and quantitation limits, robustness).
2. Sample preparation in instrumental analytical chemistry; validation of analytical procedures.

Mass-spectrometry (lectures 3-5)
1. Fundamentals of mass-spectrometry. Ion sources: Electron Impaction Ionization (EI) and Chemical Ionization (CI), Electrospray ionization (ESI), Atmospheric Pressure Chemical Ionization (APCI) and Atmospheric Pressure Photo Ionization (API). Fast atom bombardment (FAB) and Matrix Assisted Laser Desorption Ionization (MALDI) mass-spectrometry. Mass spectrometry detectors: quadrupole, ion trap, time of flight (TOF).
2. Applications of mass-spectrometry in chemistry and biochemistry

NMR spectrosc opy (lectures 6-9)
1. Fundamentals of NMR spectroscopy; sensitivity, resolution, and scope; 1D and 2D NMR methods and techniques. Principal differences between solution and solid-state NMR (magic angle spinning, chemical shift anisotropy, quadrupolar and paramagnetic spins)
2. Applications of NMR spectroscopy
Applications of NMR spectroscopy in synthetic chemistry, catalysis, and materials study

Atomic spectroscopy (lectures 10-12)
1. Fundamentals of atomic spectroscopy: atomic absorption (AA) versus atomic emission (AE/OE); principles of AA, MP-AE, ICP-OE spectroscopy and ICP-MS techniques.
2. Applications of atomic spectroscopy methods in chemistry and materials studies

Separation science (lectures 13-14)
1. Fundamentals of chromatography
2. Liquid Chromatography: modes of separation, instrumentation used, HPLC vs UHPLC. Method development for LC: optimising separation conditions, selecting an appropriate column and dete ctor for the given analysis. LC detectors: UV (photodiode array versus single wave UV), fluorescence, refractive index, electrochemical methods of detection.
3. Gas Chromatography: modes of separation, instrumentation used. Method development for GC: optimising separation conditions, selecting an appropriate column and detector for the given analysis. GC detectors: flame ionisation detector, thermal conductivity detector.
4. Applications of chromatography in chemical and pharmaceutical analysis

Hyphenated Techniques (lectures 15-16)
1. Introduction to hyphenation (GC/MS, HPLC/MS, MS/MS). GC Interfaces: Electron Impact Ionization (EI) and Chemical Ionization (CI). HPLC Interfaces: Electrospray ionization (ESI), Atmospheric Pressure Chemical Ionization (APCI) and Atmospheric Pressure Photo Ionization (APPI).
2. Applications of hyphenated techniques in chemical and pharmaceutical analysis.

Recommended Texts

Reading lists are managed at Click here to access the reading lists for this module.

Teaching Schedule

  Lectures Seminars Tutorials Lab Practicals Fieldwork Placement Other TOTAL
Study Hours 17


Timetable (if known)              
Private Study 52


EXAM Duration Timing
% of
Penalty for late
comprehensive open book examination.  90    80       
CONTINUOUS Duration Timing
% of
Penalty for late
workshops and presentation    20