Prof Craig Winstanley BSc PhD

Professor Clinical Infection, Microbiology & Immunology

Research

Research Interest 1

Current main projects:
Pseudomonas aeruginosa infections in cystic fibrosis (CF) and keratitis:
Development of diagnostic probes for Liverpool CF epidemic strain (LES) and other epidemic strains; Molecular typing of CF isolates from Liverpool and other CF units, and of eye infection isolates from across the UK; Identification of additional virulence factors in LES; Genomics and infection models to characterise genes in the LES; Analysis of P. aeruginosa population divergence and evolution in CF; Genome sequence of a keratitis isolate; Population biology of keratitis isolates; Distribution of virulence genes amongst CF and ulcerative keratitis isolates. Funding from Wellcome Trust, Dr Hadwen Trust and NIHR Biomedical Research Centre in Microbial Diseases.

Uropathogenic E.coli (UPEC) interactions with the host.
Characterising E. coli interactions with human and rat ureters. Identification of genes implicated in such interactions. Genomics of epidemiologically important UPEC. Funding from Wellcome Trust and Mersey Kidney Research.

Genetic variation in Campylobacter spp. isolates / Campylobacter in the environment
Identification of strain variations using subtractive hybridisation, microarrays and genome sequencing. Molecular epidemiology of Campylobacter. Funding from joint research councils (MRC, BBSRC, NERC, ESRC) as part of the Environment & Social Ecology of Human Infectious Diseases initiative.

Research Grants

BRONCH-UK a multicentre and multidisciplinary partnership grant tackling unmet needs in bronchiectasis.

MEDICAL RESEARCH COUNCIL (MRC)

August 2014 - July 2019

Investigating bacteria-induced Wnt signalling as a mechanism for malignant development in the gut epithelium.

NORTH WEST CANCER RESEARCH FUND

September 2013 - September 2016

Evaluation of novel therapies targeting adhesive/invasive E.Coli

DEPARTMENT OF HEALTH & SOCIAL CARE (UK) (DOH/DHSC/NIHR)

November 2007 - March 2012

Multi-centre cross Pseudomonas cross-infection study.

CHARITIES AID FOUNDATION (UK)

August 2014 - July 2015

Sources, Seasonality, Transmission and Control: Campylobacter and human behaviour in a changing environment (ESEI)

MEDICAL RESEARCH COUNCIL (MRC)

January 2012 - December 2017

ARCHIE - Early antibiotic use in ‘at risk’ children with influenza

DEPARTMENT OF HEALTH & SOCIAL CARE (UK) (DOH/DHSC/NIHR)

October 2012 - September 2020

Comparative genomics of UK Pseudomonas aeruginosa keratitis isolates to identify links between genotypic and clinical characteristics.

FIGHT FOR SIGHT (UK)

September 2014 - November 2015

Detection of minority strains of HIV harbouring drug resistance.

ROYAL LIVERPOOL AND BROADGREEN UNIVERSITY HOSPITALS NHS TRUST (UK)

October 2002 - September 2005

Clinical Exploitation of Data Produced by the Pseudomonas International Genomics Consortium.

CYSTIC FIBROSIS TRUST (UK)

October 2013 - September 2016

Ocular Mucins and infections of the Eye.

ROYAL LIVERPOOL AND BROADGREEN UNIVERSITY HOSPITALS NHS TRUST (UK)

April 2006 - January 2008

Characterisation of a type III secretion system in the Burkholderia cepacia complex.

CYSTIC FIBROSIS TRUST (UK)

October 2003 - September 2005

Response of populations of Pseudomonas aeruginosa to antimicrobial challenge during chronic infections in cystic fibrosis.

HADWEN TRUST, DR FOR HUMANE RESEARCH

October 2008 - January 2012

Veterinary Pathogen Genomics – One Genome for every 50 years of the RCVS Trust

ROYAL COLLEGE OF VETERINARY SURGEONS TRUST FUND

July 2008 - June 2011

Genetic diversity and evolution of chronic Pseudomonas aeruginosa infections in cystic fibrosis patients

WELLCOME TRUST (UK)

August 2011 - July 2015

Molecular studies of Burkholderia pseudomallei pathogenesis.

WELLCOME TRUST (UK)

January 2000 - June 2003

Novel approaches to the analysis of how uropathogenic E. coli (UPEC)-urothelium interactions impair ureteric function

WELLCOME TRUST (UK)

October 2011 - March 2013

Understanding the role of temperate bacteriophages in the ecology and evolution of Pseudomonas aeruginosa in the cystic fibrosis lung environment

WELLCOME TRUST (UK)

November 2009 - October 2012

The role of mucosa-associated cnf1-expressing Escherichia coli on colorectal tumour development and progression

NORTH WEST CANCER RESEARCH FUND

May 2006 - April 2008

Identification of emerging epidemic strains of pseudomonas aeruginosa in cystic fibrosis centres in the Liverpool area

ROYAL LIVERPOOL AND BROADGREEN UNIVERSITY HOSPITALS NHS TRUST (UK)

September 2010 - September 2011

Virulent epidemic strains of Pseudomonas aeruginosa in cystic fibrosis

DEPARTMENT OF HEALTH & SOCIAL CARE (UK) (DOH/DHSC/NIHR)

October 2008 - April 2012

The role of type III secretion in Burkholderia cepacia pathogenesis in cystic fibrosis.

CYSTIC FIBROSIS TRUST (UK)

March 2000 - June 2002

Use of Subtractive Hybridisation to Identify Novel Genomic Regions of an Epidemic Strain of Pseudomonas Aeruginosa.

CYSTIC FIBROSIS TRUST (UK)

March 2002 - September 2005

Identifying the molecular mechanisms of interaction between colonic Crohn's disease mucosa-associated Escherichia coli and the intestinal epithelium

NAT ASSOC FOR COLITIS & CROHNS DISEASE (UK)

May 2008 - April 2010

Use of artificial sputum medium to study antimicrobial susceptibility of Pseudomonas aeruginosa biofilm populations of relevance to cystic fibrosis

NOVARTIS PHARMACEUTICALS UK LTD (UK)

May 2010 - October 2010

How does bacterial infection cause ureteric dysfunction and contribute to kidney damage.

MERSEY KIDNEY FIRST (UK)

October 2008 - October 2011

Investing the contribution of mucosa-associated Escherichia coli isolated from colon cancer on tumour development and progression.

NORTH WEST CANCER RESEARCH FUND

May 2005 - April 2006

Origins transmission pathways and emergence of human campylobacter infections (Campylobacter).

DEPARTMENT FOR ENVIRONMENT, FOOD AND RURAL AFFAIRS (UK)

April 2004 - December 2009

Contact-network studies between dogs, and between dogs and humans.

VETERINARY LABORATORIES AGENCY (UK)

October 2004 - April 2008

UK CF Microbiology Consortium - Programme 1: Genetic Factors contributing to the success of CF 'superbugs' in the United Kingdom.

CYSTIC FIBROSIS TRUST (UK)

October 2005 - September 2008

Fully Integrated, real-time detection, diagnosis and control of community infection outbreaks.

WELLCOME TRUST (UK)

April 2013 - July 2018

Application of whole genome sequencing to fully characterise Campylobacter isolates from the IID1 and IID2 studies.

FOOD STANDARDS AGENCY (UK)

January 2014 - February 2015

Infections of the eye – bacterial keratitis

ROYAL LIVERPOOL AND BROADGREEN UNIVERSITY HOSPITALS NHS TRUST (UK)

September 2010 - August 2011