Tick cell lines

The table below details the tick cell lines currently available for distribution.

As well as tick cell lines established at the University of Edinburgh between 1980 and 2012, and at The Pirbright Institute between 2012 and 2017, the Biobank holds many cell lines deposited by Professors Ulrike Munderloh and Timothy Kurtti of the University of Minnesota and Dr Patricia Holman of Texas A&M University. In addition we hold two of the original Rhipicephalus appendiculatus cell lines (RA243 and RA257) established at the London School of Hygiene and Tropical Medicine over 40 years ago by Varma, Pudney and Leake (1975), and a Rhipicephalus sanguineus cell line set up at Rocky Mountain Laboratories, Montana, USA in the late 1970s. The exact original designation of the latter line is unclear so it is currently named “RML-RSE” for convenience.”

 

 

Type Species Origin Line Instar Passage Temperature Reference
Hard Amblyomma americanum United States AAE2 Embryo ~27 32°C - 34°C Kurtti et al., 2005
Hard Amblyomma americanum United States AAE12 Embryo ~8 32°C - 34°C Singu et al., 2006
Hard Amblyomma variegatum Southern Africa AVL/CTVM13 Moulting larva ~137 28 - 32°C, 37°C Bell-Sakyi et al., 2000
Hard Amblyomma variegatum Southern Africa AVL/CTVM17 Moulting larva ~163 32°C Bell-Sakyi, 2004
Soft Carios capensis United States CCE1 Embryo ~120 32°C - 34°C Mattila et al., 2007
Soft Carios capensis United States CCE2 Embryo ~102 32°C - 34°C Mattila et al., 2007
Hard Dermacentor andersoni United States DAE15 Embryo ~55 32°C - 34°C Simser et al., 2001
Hard Dermacentor andersoni United States DAE100T Embryo ~60 32°C - 34°C Kurtti et al., 2005
Hard Dermacentor (Anocentor) nitens United States ANE58 Embryo ~15 32°C - 34°C Kurtti and Munderloh, 1983
Hard Dermacentor variabilis United States DVE1 Embryo ~10 32°C - 34°C Kurtti et al., 2005
Hard Dermacentor albipictus United States DALBE3 Embryo ~15 32°C - 34°C Policastro et al., 1997
Hard Hyalomma anatolicum India HAE/CTVM8 Embryo ~150 32°C Bell-Sakyi, 1991
Hard Hyalomma anatolicum anatolicum India HAE/CTVM9 Embryo ~199 32°C Bell-Sakyi, 1991
Hard Ixodes ricinus United Kingdom IRE/CTVM19 Embryo ~224 28°C Bell-Sakyi et al., 2007
Hard Ixodes ricinus United Kingdom IRE/CTVM20 Embryo ~212 28°C Bell-Sakyi et al., 2007
Hard Ixodes ricinus Germany IRE11 Embryo ~55 32°C - 34°C Simser et al., 2002
Hard Ixodes scapularis United States IDE2 Embryo ~75 32°C - 34°C Munderloh et al., 1994
Hard Ixodes scapularis United States IDE8 Embryo ~93 32°C - 34°C Munderloh et al., 1994
Hard Ixodes scapularis United States ISE6 Embryo ~80 32°C - 34°C Munderloh et al., 1994
Hard Ixodes scapularis United States ISE18 Embryo ~42 32°C - 34°C Munderloh et al., 1994
Soft Ornithodoros moubata Kenya OME/CTVM21 Embryo/neonate larva ~52 28°C Bell-Sakyi et al., 2009
Soft Ornithodoros moubata Kenya OME/CTVM22 Embryo/neonate larva ~44 28°C Bell-Sakyi et al., 2009
Soft Ornithodoros moubata Kenya OME/CTVM24 Embryo/neonate larva ~44 28°C Bell-Sakyi et al., 2009
Soft Ornithodoros moubata Kenya OME/CTVM27 Embryo/neonate larva ~32 28°C Bell-Sakyi et al., 2009
Hard Rhipicephalus appendiculatus Kenya RAE/CTVM1 Embryo ~102 32°C Bell-Sakyi, 2004
Hard Rhipicephalus appendiculatus Kenya RAN/CTVM3 Moulting nymph ~60 28°C Bekker et al., 2002
Hard Rhipicephalus appendiculatus Kenya RAE25 Embryo ~131 28°C - 34°C Kurtti et al., 1982
Hard Rhipicephalus appendiculatus East Africa RA243 Moulting nymph ~350 28°C - 32°C Varma et al., 1975
Hard Rhipicephalus appendiculatus East Africa RA257 Moulting nymph ~250 28°C - 32°C Varma et al., 1975
Hard Rhipicephalus evertsi South Africa REE/CTVM28 Embryo ~41 28°C Alberdi et al., 2012
Hard Rhipicephalus evertsi South Africa REE/CTVM29 Embryo ~29 28°C Alberdi et al., 2012
Hard Rhipicephalus evertsi South Africa REE/CTVM31 Embryo ~19 28°C Alberdi et al., 2012
Hard Rhipicephalus evertsi South Africa REN/CTVM32 Moulting nymph ~22 28°C Bell-Sakyi et al., 2015
Hard Rhipicephalus sanguineus United States RSE8 Embryo ~21 32°C - 34°C Kurtti and Munderloh, 1982
Hard Rhipicephalus sanguineus United States RML-RSE Embryo ~87 28°C - 32°C Yunker et al.
Hard Rhipicephalus (Boophilus) decoloratus Kenya BDE/CTVM14 Embryo ~58 28°C Lallinger et al., 2010
Hard Rhipicephalus (Boophilus) decoloratus Kenya BDE/CTVM16 Embryo ~94 28°C - 32°C Bell-Sakyi, 2004
Hard Rhipicephalus (Boophilus) microplus Costa Rica BME/CTVM2 Embryo ~145 28°C - 32°C Bell-Sakyi, 2004
Hard Rhipicephalus (Boophilus) microplus Costa Rica BME/CTVM4 Embryo ~150 28°C Bell-Sakyi et al., 2007
Hard Rhipicephalus (Boophilus) microplus Colombia BME/CTVM5 Embryo ~77 28°C Bell-Sakyi et al., 2007
Hard Rhipicephalus (Boophilus) microplus Colombia BME/CTVM6 Embryo ~221, 243 28°C - 32°C Bell-Sakyi, 2004
Hard Rhipicephalus (Boophilus) microplus Mozambique BME/CTVM23 Embryo ~70 32°C Alberdi et al., 2012
Hard Rhipicephalus (Boophilus) microplus Mozambique BME/CTVM30 Embryo ~32 28°C Alberdi et al., 2012 
Hard Rhipicephalus (Boophilus) microplus Mexico BmVIII-SCC Embryo ~47 32°C Holman, 1981