Tick cell lines

The table below details the tick cell lines currently available for distribution.

As well as tick cell lines established at the University of Edinburgh between 1980 and 2012, and at The Pirbright Institute between 2012 and 2017, the Tick Cell Biobank holds many cell lines deposited by Professors Ulrike Munderloh and Timothy Kurtti of the University of Minnesota and Dr Patricia Holman of Texas A&M University. In addition we hold two of the original Rhipicephalus appendiculatus cell lines (RA243 and RA257) established at the London School of Hygiene and Tropical Medicine over 40 years ago by Varma, Pudney and Leake (1975), and two deposits of the RML-15 cell line set up at Rocky Mountain Laboratories, Montana, USA in the late 1970s by Yunker, Cory and Meibos (1985). RML-15 was published as originating from Dermacentor variabilis embryos, but both of our deposits were found to be derived from Rhipicephalus sanguineus; as the exact origin of these cells is unclear and we do not know if they were derived from the same or different primary cultures, for convenience we refer to the low passage deposit as “RML-15” and the high passage deposit as “RML-RSE”.

Other arthropod cell lines

Our remit also includes storage and development of cell lines from other arthropod species such as biting midges, sand flies, mosquitoes, tsetse flies, kissing bugs, bees and mites. We currently have cell lines derived from one midge and one sand fly species available for distribution; these are also listed in the table below.

 

Arthropod group

Species

Origin

Line

Instar

Approx. passage

Incubation temperature

Reference

Hard tick

Amblyomma americanum

United States

AAE2

Embryo

28

32°C - 34°C

Kurtti et al., 2005

Hard tick

Amblyomma americanum

United States

AAE12

Embryo

8

32°C - 34°C

Singu et al., 2006

Hard tick

Amblyomma variegatum

Southern Africa

AVL/CTVM13

Moulting larva

150

28 - 32°C, 37°C

Bell-Sakyi et al., 2000

Hard tick

Amblyomma variegatum

Southern Africa

AVL/CTVM17

Moulting larva

123

32°C

Bell-Sakyi, 2004

Soft tick

Argas reflexus

Germany

ARE/LULS41

Embryo

14

28°C

Bell-Sakyi et al., 2018

Soft tick

Carios capensis

United States

CCE1

Embryo

132

32°C - 34°C

Mattila et al., 2007

Soft tick

Carios capensis

United States

CCE2

Embryo

112

32°C - 34°C

Mattila et al., 2007

Biting midge

Culicoides sonorensis

United States

KC

Embryo

62

28°C

Wechsler et al., 1991

Hard tick

Dermacentor andersoni

United States

DAE15

Embryo

55

32°C - 34°C

Simser et al., 2001

Hard tick

Dermacentor andersoni

United States

DAE100T

Embryo

60

32°C - 34°C

Kurtti et al., 2005

Hard tick

Dermacentor (Anocentor) nitens

United States

ANE58

Embryo

15

32°C - 34°C

Kurtti and Munderloh, 1983

Hard tick

Dermacentor variabilis

United States

DVE1

Embryo

10

32°C - 34°C

Kurtti et al., 2005

Hard tick

Dermacentor albipictus

United States

DALBE3

Embryo

15

32°C - 34°C

Policastro et al., 1997

Hard tick

Hyalomma anatolicum

India

HAE/CTVM8

Embryo

163

32°C

Bell-Sakyi, 1991

Hard tick

Hyalomma anatolicum

India

HAE/CTVM9

Embryo

212

32°C

Bell-Sakyi, 1991

Hard tick

Hyalomma dromedarii

East Africa

HDE/PIPA33

Embryo

20

28°C

Bell-Sakyi et al., 2018

Hard tick

Hyalomma dromedarii

East Africa

HDE/LURF39

Embryo

12

28°C

Bell-Sakyi et al., 2018

Hard tick

Hyalomma lusitanicum

Spain

HLE/LULS42

Embryo

8

28°C

Bell-Sakyi et al., 2018

Hard tick

Ixodes ricinus

United Kingdom

IRE/CTVM19

Embryo

230

28°C

Bell-Sakyi et al., 2007

Hard tick

Ixodes ricinus

United Kingdom

IRE/CTVM20

Embryo

180

28°C

Bell-Sakyi et al., 2007

Hard tick

Ixodes ricinus

Germany

IRE11

Embryo

56

32°C - 34°C

Simser et al., 2002

Hard tick

Ixodes scapularis

United States

IDE2

Embryo

86

32°C - 34°C

Munderloh et al., 1994

Hard tick

Ixodes scapularis

United States

IDE8

Embryo

102

32°C - 34°C

Munderloh et al., 1994

Hard tick

Ixodes scapularis

United States

ISE6

Embryo

83

32°C - 34°C

Munderloh et al., 1994

Hard tick

Ixodes scapularis

United States

ISE18

Embryo

55

32°C - 34°C

Munderloh et al., 1994

Sand fly

Lutzomyia longipalpis

Brazil

LLE/LULS40

Embryo

50

28°C

Bell-Sakyi et al., 2018

Soft tick

Ornithodoros moubata

Kenya

OME/CTVM21

Embryo/neonate larva

60

28°C

Bell-Sakyi et al., 2009

Soft tick

Ornithodoros moubata

Kenya

OME/CTVM22

Embryo/neonate larva

60

28°C

Bell-Sakyi et al., 2009

Soft tick

Ornithodoros moubata

Kenya

OME/CTVM24

Embryo/neonate larva

59

28°C

Bell-Sakyi et al., 2009

Soft tick

Ornithodoros moubata

Kenya

OME/CTVM27

Embryo

47

28°C

Bell-Sakyi et al., 2009

Hard tick

Rhipicephalus appendiculatus

Kenya

RAE/CTVM1

Embryo

95

32°C

Bell-Sakyi, 2004

Hard tick

Rhipicephalus appendiculatus

Kenya

RAN/CTVM3

Moulting nymph

70

28°C

Bekker et al., 2002

Hard tick

Rhipicephalus appendiculatus

Kenya

RAE/PIPM38

Embryo

7

28°C

Bell-Sakyi et al., 2018

Hard tick

Rhipicephalus appendiculatus

Kenya

RAE25

Embryo

143

28°C - 34°C

Kurtti et al., 1982

Hard tick

Rhipicephalus appendiculatus

East Africa

RA243

Moulting nymph

358

28°C - 32°C

Varma et al., 1975

Hard tick

Rhipicephalus appendiculatus

East Africa

RA257

Moulting nymph

264

28°C - 32°C

Varma et al., 1975

Hard tick

Rhipicephalus evertsi

South Africa

REE/CTVM28

Embryo

53

28°C

Alberdi et al., 2012

Hard tick

Rhipicephalus evertsi

South Africa

REE/CTVM29

Embryo

8

28°C

Alberdi et al., 2012

Hard tick

Rhipicephalus evertsi

South Africa

REE/CTVM31

Embryo

32

28°C

Alberdi et al., 2012

Hard tick

Rhipicephalus evertsi

South Africa

REN/PIPA34

Moulting nymph

20

28°C

Bell-Sakyi et al., 2018

Hard tick

Rhipicephalus sanguineus

United States

RSE8

Embryo

34

32°C - 34°C

Kurtti and Munderloh, 1982

Hard tick

Rhipicephalus sanguineus

United States

RML-RSE

Embryo

100

28°C - 32°C

Yunker et al. 1981; Bell-Sakyi et al., 2015

Hard tick

Rhipicephalus sanguineus

United States

RML-15

Embryo

20

28°C

Yunker et al. 1981; Bell-Sakyi et al., 2018

Hard tick

Rhipicephalus sanguineus

France

RSE/PILS35

Embryo

20

28°C

Koh-Tan et al., 2016

Hard tick

Rhipicephalus (Boophilus) decoloratus

Kenya

BDE/CTVM14

Embryo

100

28°C

Lallinger et al., 2010

Hard tick

Rhipicephalus (Boophilus) decoloratus

Kenya

BDE/CTVM16

Embryo

106

28°C - 32°C

Bell-Sakyi, 2004

Hard tick

Rhipicephalus (Boophilus) microplus

Costa Rica

BME/CTVM2

Embryo

160

28°C - 32°C

Bell-Sakyi, 2004

Hard tick

Rhipicephalus (Boophilus) microplus

Costa Rica

BME/CTVM4

Embryo

150

28°C

Bell-Sakyi et al., 2007

Hard tick

Rhipicephalus (Boophilus) microplus

Colombia

BME/CTVM5

Embryo

90

28°C

Bell-Sakyi et al., 2007

Hard tick

Rhipicephalus (Boophilus) microplus

Colombia

BME/CTVM6

Embryo

250

28°C - 32°C

Bell-Sakyi, 2004

Hard tick

Rhipicephalus (Boophilus) microplus

Mozambique

BME/CTVM23

Embryo

77

32°C

Alberdi et al., 2012

Hard tick

Rhipicephalus (Boophilus) microplus

Mozambique

BME/CTVM30

Embryo

45

28°C

Alberdi et al., 2012 

Hard tick

Rhipicephalus (Boophilus) microplus

Mexico

BmVIII-SCC

Embryo

65

32°C

Holman, 1981

Hard tick

Rhipicephalus (Boophilus) microplus

Brazil

BME/PIBB36

Embryo

10

28°C

Bell-Sakyi et al., 2018

Hard tick

Rhipicephalus (Boophilus) microplus

Mexico

BME26

Embryo

88

32°C

Kurtti et al., 1988