GCRF-BBR: The Tick Cell Biobank: outposts in Asia, Africa and South America.
(BBSRC grant no. BB/P024378/1)
This 3-year project funded by the BBSRC Global Challenges Research Fund (GCRF) BBSRC-CGRF Funding will establish outposts of the parent Tick Cell Biobank in Malaysia, Kenya and Brazil. The aim is to facilitate uptake of tick cell lines by researchers in South-East Asia, Africa and South America through local access to cell lines and associated training. This is needed because, while there is a year-on-year increase in scientific publications reporting use of tick cell lines, and on average over half of these deal with tick and tick-borne disease problems of lower-and-middle-income countries (LMIC), only a tiny proportion of this research is actually carried out in LMIC laboratories.
Numbers of publications using tick cell lines (blue) that deal with LMIC ticks and tick cell disease problems (red)
Proportion of publications dealing with ticks and tick-borne disease problems that issue from LMIC (red) and high-income countries (blue)
Better access to tick cell lines and associated expertise will enable LMIC scientists to exploit these unique tools in their research, assisting them to deliver locally-generated solutions to their tick and tick-borne disease problems affecting human and animal health.
Each Outpost will be stocked with a panel of the internationally most popular tick cell lines originating from the parent Tick Cell Biobank and the University of Minnesota, plus cell lines derived from regionally-relevant tick species. The Outposts will supply tick cell lines to scientists within their geographical area, thereby reducing access costs and logistical difficulties compared to sourcing tick cells from the UK, but subject to the same Material Transfer Agreement conditions. Each outpost will provide locally-tailored training for recipient scientists to facilitate successful transfer and exploitation of tick cells in their laboratories. Additionally, each Outpost will aim to establish novel cell lines from local tick species or geographical strains not already represented in the collection, either by themselves or in collaboration with other project members or local scientists.
Tick Cell Biobank Outpost teams:
TCB Asia is being established at the University of Malaya in Kuala Lumpur, Malaysia, and will be based in the Tropical Infectious Diseases Research and Education Centre (TIDREC) http://www.tidrec.com/. The team comprises Prof. Sazaly AbuBakar (PI), Dr Jing Jing Khoo and Mrs Siti Sarah Nor'e
TCB Africa will be hosted by the Tick Unit at the International Livestock Research Institute (ILRI) in Nairobi, Kenya, and will be run by Dr Naftaly Githaka (PI) and Mr Milton Owido
TCB South America
TCB South America will be established at the Oswaldo Cruz Institute (Fiocruz) https://portal.fiocruz.br/en in Rio de Janeiro, Brazil and run by Dr Flavio Alves Lara (PI) and Mr Diego Oliveira. The third member of the Brazilian team, Prof. Adivaldo Fonseca (Co-PI) of the Federal Rural University of Rio de Janeiro (UFRRJ) is providing advice, expertise and support to the Outpost.
Progress in the first and second years:
The Outposts project commenced in July 2017; we held a start-up meeting (December 2017) and a training workshop in Biobank management for the Outpost teams (April 2018) in Liverpool. Each team was involved in preliminary planning (identifying suitable dedicated laboratory space, obtaining estimates for equipment etc.) in conjunction with Liverpool staff who visited each Outpost during the second year. The next phase of the project involved equipping each Outpost with the required dedicated equipment and consumables and putting in place the necessary administrative platforms, before stocking the Outposts with the selected tick cell lines.
Progress in the third year:
In the third year, each Outpost is holding an initial training workshop for local scientists, assisted by the Liverpool team. The first of these workshops, hosted by the TCB South America team at Fiocruz on 29th-31st July 2018, was attended by twelve young scientists – ten from Brazil and one each from Argentina and Uruguay. Following the workshop, several of the scientists have already requested and received tick cell lines from the Brazil Outpost for their research.