Gram-negative Bacterial Pathogens
Current main projects
Pseudomonas aeruginosa infections in cystic fibrosis (CF) and keratitis:
- Development of diagnostic probes for Liverpool CF epidemic strain (LES) and other epidemic strains;
- Molecular typing of CF isolates from Liverpool and other CF units, and of eye infection isolates from across the UK; Identification of additional virulence factors in LES;
- Genomics and infection models to characterise genes in the LES;
- Biofilm models for screening anti-infective agents and modelling bacterial populations
- Analysis of P. aeruginosa population divergence and evolution in CF; Role of bacteriophages in strain evolution/adaptation
- Population biology of keratitis isolates; Distribution of virulence genes amongst CF and ulcerative keratitis isolates.
- Funding from Wellcome Trust, Dr Hadwen Trust and NIHR Biomedical Research Centre in Microbial Diseases.
Microbiomes / bacterial population interactions
- Next generation sequencing microbiome analysis applied to human respiratory samples
- Bacterial interactions in mixed biofilms
- Funding from NIHR and Leverhulme Trust
Uropathogenic E.coli (UPEC) interactions with the host
- Characterising E. coli interactions with human and rat ureters. Identification of genes implicated in such interactions.
- Genomics of epidemiologically important UPEC.
- Funding from Wellcome Trust and Mersey Kidney Research.
Genetic variation in Campylobacter spp. isolates / Campylobacter in the environment
- Identification of strain variations using subtractive hybridisation, microarrays and genome sequencing.
- Molecular epidemiology of Campylobacter.
- Population biology of Campylobacter spp. in the environment
- Funding from joint research councils (MRC, BBSRC, NERC, ESRC) as part of the Environment & Social Ecology of Human Infectious Diseases initiative.
Characterisation of mucosal isolates of Escherichia coli in relation to Crohn’s disease and colon cancer
- Studies aimed at characterising the role of E. coli in Crohn’s disease / colon cancer; Identification of novel genes; Characterisation of the role of CNF-1.
- Funding from North West Cancer Research Fund, NACC and NIHR.
Genomics of pathogens of veterinary importance, Genome sequencing of 50 pathogens
- Genomics of P. aeruginosa in ovine/bovine mastitis.
- Comparative genomics of C. fetus
- Funding from RCVS Trust.
Genomic analysis of invasive African non-typhoidal salmonellae
- Identification of novel virulence genes using subtractive hybridisation; Genome sequence analysis / comparative genomics.
- Funding from MRC.